{"id":119955,"date":"2024-07-29T10:49:21","date_gmt":"2024-07-29T08:49:21","guid":{"rendered":"https:\/\/ibecbarcelona.eu\/new-bioink-to-mimic-human-skin-constructs-using-3d-bioprinting\/"},"modified":"2024-09-02T15:45:49","modified_gmt":"2024-09-02T13:45:49","slug":"nova-biotinta-dissenyada-per-a-la-construir-pell-humana-amb-enginyeria-de-teixits","status":"publish","type":"post","link":"https:\/\/ibecbarcelona.eu\/ca\/nova-biotinta-per-a-sintetitzar-pell-humana-amb-bioimpresion-3d\/","title":{"rendered":"Nova biotinta per a sintetitzar pell humana mitjan\u00e7ant bioimpresi\u00f3 3D\u00a0"},"content":{"rendered":"\n<h4 class=\"wp-block-heading\">La demanda de teixits de pell sintetitzats en laboratori ha augmentat tant per a aplicacions <em>in vitro<\/em> com <em>in vivo<\/em>, i un dels punts clau en aquest proc\u00e9s \u00e9s l&#8217;elecci\u00f3 del material adequat per a la \u201cbastida\u201d sobre la qual es construir\u00e0 el teixit. En aquest escenari, investigadores de l&#8217;IBEC acaben de publicar un treball que descriu una nova biotinta dissenyada per a la construir pell humana a trav\u00e9s d&#8217;enginyeria de teixits utilitzant hidrogels basats en norborn\u00e9-pululan.&nbsp;<\/h4>\n\n\n\n<figure class=\"wp-block-image size-large\"><img decoding=\"async\" width=\"1024\" height=\"768\" data-src=\"https:\/\/ibecbarcelona.eu\/wp-content\/uploads\/2024\/07\/image-2-1024x768.jpeg\" alt=\"\" class=\"wp-image-119949 lazyload\" data-srcset=\"https:\/\/ibecbarcelona.eu\/wp-content\/uploads\/2024\/07\/image-2-1024x768.jpeg 1024w, https:\/\/ibecbarcelona.eu\/wp-content\/uploads\/2024\/07\/image-2-300x225.jpeg 300w, https:\/\/ibecbarcelona.eu\/wp-content\/uploads\/2024\/07\/image-2-768x576.jpeg 768w, https:\/\/ibecbarcelona.eu\/wp-content\/uploads\/2024\/07\/image-2.jpeg 1143w\" data-sizes=\"(max-width: 1024px) 100vw, 1024px\" src=\"data:image\/svg+xml;base64,PHN2ZyB3aWR0aD0iMSIgaGVpZ2h0PSIxIiB4bWxucz0iaHR0cDovL3d3dy53My5vcmcvMjAwMC9zdmciPjwvc3ZnPg==\" style=\"--smush-placeholder-width: 1024px; --smush-placeholder-aspect-ratio: 1024\/768;\" \/><figcaption class=\"wp-element-caption\">Investigadores del grup de Sistemes Biomim\u00e9ticos per a Enginyeria Cel\u00b7lular de l&#8217;IBEC (d&#8217;esquerra a dreta): Angela Cirulli, N\u00faria Torras i Elena Mart\u00ednez.&nbsp;<\/figcaption><\/figure>\n\n\n\n<p>Els teixits cutanis obtinguts amb bioenginyeria tenen aplicacions vers\u00e0tils en entorns cl\u00ednics, com la substituci\u00f3 de pell i els ap\u00f2sits avan\u00e7ats per a ferides, aix\u00ed com en escenaris precl\u00ednics per a testejar nous f\u00e0rmacs, desenvolupar models de malalties i realitzar assajos amb cosm\u00e8tics. Un teixit de pell sintetitzat per bioenginyeria ha de ser f\u00e0cil de preparar, resistent mec\u00e0nicament i propici per a l&#8217;adhesi\u00f3 i el creixement cel\u00b7lular, tant en el compartiment epid\u00e8rmic com en el d\u00e8rmic. El teixit ha de mantenir un equilibri entre elasticitat i estabilitat mec\u00e0nica, i alhora, proporcionar un microambient cel\u00b7lular adequat per a contenir els diferents compartiments de la pell humana.&nbsp;<\/p>\n\n\n\n<p>Recentment, investigadores liderades per Elena Mart\u00ednez del grup de Sistemes Biomim\u00e8tics per a Enginyeria Cel\u00b7lular de l&#8217;IBEC, han publicat en la revista International Journal of Bioprinting el treball &#8220;<a href=\"https:\/\/accscience.com\/journal\/IJB\/0\/0\/10.36922\/ijb.3395\" target=\"_blank\" rel=\"noreferrer noopener\">Mimicing human skin constructs using norbornene-pullulan-based hydrogels<\/a>&#8220;, que ajudar\u00e0 a abordar i resoldre aquestes q\u00fcestions. En concret, van presentar una nova biotinta entrecreauble mitjan\u00e7ant llum dissenyada per a sintetitzar pell humana, basada en formulacions de tiol-norborn\u00e9-pululan (N-PLN) combinades amb diversos fotopolimeritzadors.\u00a0Aquest treball s&#8217;ha desenvolupat en el marc del projecte europeu B-BRIGHTER\u00a0(No. 101057894).<\/p>\n\n\n\n<p>Aquesta nova biotinta es va utilitzar de manera efectiva en una bioimpresora d\u2019escriptura per l\u00e0ser desenvolupada al laboratori per a generar matrius d\u00e8rmiques epitel\u00b7lialitzades. El compartiment d\u00e8rmic es va formar mitjan\u00e7ant la fotopolimetitzaci\u00f3 d&#8217;una soluci\u00f3 de pre-pol\u00edmer que contenia fibroblasts humans, mentre que el compartiment epid\u00e8rmic es va obtenir sembrant queratin\u00f2cits humans en els hidrogels pr\u00e8viament generats carregats de fibroblasts. Utilitzant llum visible, es van poder imprimir hidrogels de 2,5 mm\u00b3 carregats de c\u00e8l\u00b7lules, en nom\u00e9s 10 segons. La qu\u00edmica de fotopolimetitzaci\u00f3 mitjan\u00e7ant grups thiol emprada en aquest estudi va crear una matriu extracelul\u00b7lar ben definida amb reticulacions ortogonals, mantenint altes taxes de viabilitat cel\u00b7lular per a fibroblasts encapsulats.&nbsp;<\/p>\n\n\n\n<p>Aquest estudi suposa una estrat\u00e8gia prometedora per a desenvolupar un model de pell humana utilitzant un sistema de bioimpresi\u00f3n 3D basat en llum fet a mida, i emprant nous pol\u00edmers N-PLN. Aquests hidrogels, formats despr\u00e9s d&#8217;una breu exposici\u00f3 a la llum visible a baixa dosi, exhibeixen les propietats fisicoqu\u00edmiques necess\u00e0ries per a proporcionar una excel\u00b7lent viabilitat, proliferaci\u00f3 i elongaci\u00f3 cel\u00b7lular, aix\u00ed com la secreci\u00f3 de prote\u00efnes de matriu extracel\u00b7lular, crucials per a la correcta formaci\u00f3 de teixits. A m\u00e9s, els hidrogels carregats de fibroblasts donen suport a l\u2019adhesi\u00f3 i creixement dels queratin\u00f2cits damunt, la qual cosa permet la formaci\u00f3 de matrius d\u00e8rmiques epitel\u00b7lialitzades.&nbsp;<\/p>\n\n\n\n<p>Aquest m\u00e8tode representa un prometedor punt de partida per al desenvolupament de constructes de pell humana basats en hidrogels fotopolimeritzables utilitzant la qu\u00edmica del tiol-norborn\u00e9, obrint cam\u00ed per a la creaci\u00f3 de complexos models <em>in vitro<\/em> de teixits humans. La combinaci\u00f3 de la fotopolimeritzaci\u00f3 amb llum visible amb materials basats en N-PLN, representen una clara alternativa per al&nbsp; desenvolupament de models de pell que siguin f\u00e0cils d&#8217;utilitzar i r\u00e0pids de sintetitzar, reprodu\u00efbles i econ\u00f2micament viables rendibles, cosa fet que podria ser particularment benefici\u00f3s per assajos precl\u00ednics <em>in vitro<\/em> en recerca cosm\u00e8tica i farmac\u00e8utica.&nbsp;<\/p>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n\n\n\n<p><strong>Article de refer\u00e8ncia:<\/strong><\/p>\n\n\n\n<p>Angela Cirulli, Livia Neves Borgheti-Cardoso, N\u00faria Torras, Elena Mart\u00ednez. <strong>Mimicking human skin constructs using norbornene-pullulan-based hydrogels. <\/strong><em>International Journal of Bioprinting<\/em> (2024) DOI: <a href=\"https:\/\/doi.org\/10.36922\/ijb.3395\" target=\"_blank\" rel=\"noreferrer noopener\">https:\/\/doi.org\/10.36922\/ijb.3395<\/a>\u00a0<\/p>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n","protected":false},"excerpt":{"rendered":"<p>La demanda de teixits de pell sintetitzats en laboratori ha augmentat tant per a aplicacions in vitro com in vivo, i un dels punts clau en aquest proc\u00e9s \u00e9s l&#8217;elecci\u00f3 &#8230; <a title=\"Nova biotinta per a sintetitzar pell humana mitjan\u00e7ant bioimpresi\u00f3 3D\u00a0\" class=\"read-more\" href=\"https:\/\/ibecbarcelona.eu\/ca\/nova-biotinta-per-a-sintetitzar-pell-humana-amb-bioimpresion-3d\/\" aria-label=\"More on Nova biotinta per a sintetitzar pell humana mitjan\u00e7ant bioimpresi\u00f3 3D\u00a0\">Read more<\/a><\/p>\n","protected":false},"author":4,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[96],"tags":[1767,1826,820],"post_formats":[],"class_list":["post-119955","post","type-post","status-publish","format-standard","hentry","category-noticies-de-recerca","tag-bioimpressio-3d-ca","tag-biomimetic-systems-for-cell-engineering-ca","tag-elena-martinez-ca"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.4 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Nova biotinta per a sintetitzar pell humana mitjan\u00e7ant bioimpresi\u00f3 3D\u00a0 - Institute for Bioengineering of Catalonia<\/title>\n<meta name=\"description\" content=\"investigadores de l&#039;IBEC han publicat un treball que descriu una nova biotinta dissenyada per a la construir pell humana amb enginyeria de teixits.\" \/>\n<meta name=\"robots\" content=\"index, follow, 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