{"id":23264,"date":"2016-06-03T15:54:08","date_gmt":"2016-06-03T15:54:08","guid":{"rendered":"http:\/\/ibecbarcelona.eu\/?p=23264"},"modified":"2016-06-10T09:57:32","modified_gmt":"2016-06-10T09:57:32","slug":"investigadores-logran-rastrear-celulas-en-tejidos-profundos-3","status":"publish","type":"post","link":"https:\/\/ibecbarcelona.eu\/ca\/investigadores-logran-rastrear-celulas-en-tejidos-profundos-3\/","title":{"rendered":"Investigadors aconsegueixen rastrejar c\u00e8l\u00b7lules en teixits profunds"},"content":{"rendered":"
\"CorazonImatge: Cor de peix zebra adult, en verd la descend\u00e8ncia de les c\u00e8l\u00b7lules marcades (esquerra). Ampliaci\u00f3 de la zona marcada, mostrant les c\u00e8l\u00b7lules card\u00edaques fotoactivades en verd, entremesclades amb c\u00e8l\u00b7lules sense marcar (dreta).<\/h6>\n

La manipulaci\u00f3 de l’expressi\u00f3 de prote\u00efnes per fer un seguiment del comportament cel\u00b7lular \u00e9s una potent eina en el camp de la biologia. Fins ara, l’expressi\u00f3 de prote\u00efnes en c\u00e8l\u00b7lules seleccionades s’activava mitjan\u00e7ant 2 fotons, per\u00f2 el seu \u00fas quedava limitat a c\u00e8l\u00b7lules properes a la superf\u00edcie a causa de l’elevada dispersi\u00f3 dels fotons vermells utilitzats. No obstant aix\u00f2, per investigar els \u00f2rgans interns durant el desenvolupament \u00e9s necessari, no solament mantenir la viabilitat de les c\u00e8l\u00b7lules marcades, sin\u00f3 poder accedir a aquestes quan es troben emmascarades en teixits m\u00e9s complexos o profunds.<\/p>\n

“Aquesta innovadora t\u00e8cnica, basada en la fotoactivaci\u00f3 de tres fotons de longitud d’ona m\u00e9s llarga, suposa una soluci\u00f3 al problema de la dispersi\u00f3 i, a m\u00e9s, \u00e9s capa\u00e7 d’activar les prote\u00efnes que es troben en les c\u00e8l\u00b7lules dels teixits m\u00e9s profunds sense interferir en l’esperan\u00e7a de vida de l’animal”, comenta Dobryna Zalvidea, responsable de l’estudi en l’Institut de Bioinginyeria de Catalunya (IBEC).<\/p>\n

Els investigadors van marcar les c\u00e8l\u00b7lules musculars del cor d’embrions de peix zebra i van descobrir que la prog\u00e8nie d’aquestes c\u00e8l\u00b7lules en peixos adults estava marcada de manera permanent sense causar cap dany remarcable als teixits. Aquesta metodologia, tamb\u00e9 pot estendre’s a altres teixits i models animals, aix\u00ed com ser d’utilitada per activar, amb resoluci\u00f3 espacial i temporal, l’expressi\u00f3 de diferents prote\u00efnes per controlar el comportament a llarg termini de c\u00e8l\u00b7lules individuals o de grups cel\u00b7lulars in vivo<\/em>.<\/p>\n

El sistema proposat en aquest estudi basat en la fotoactivaci\u00f3 utilitza un inductor inactiu encapsulat (Cre-loxP) que penetra a l’interior de tot l’organisme per\u00f2 que solament es torna funcional quan s’activa mitjan\u00e7ant la llum. Una vegada activat, l’inductor \u00e9s capa\u00e7 de modificar llocs determinats de l’ADN de les c\u00e8l\u00b7lules de les quals es vol con\u00e8ixer el seu comportament. D’aquesta manera, es controla l’expressi\u00f3 de les prote\u00efnes i es poden dirigir els canvis a tipus cel\u00b7lulars espec\u00edfics utilitzant \u00fanicament un est\u00edmul extern com a desencadenant. Per\u00f2 com es tracta d’una modificaci\u00f3 gen\u00e8tica aquesta persisteix durant tota la vida de la c\u00e8l\u00b7lula i, a m\u00e9s, es transmet a tota la seva descend\u00e8ncia.<\/p>\n

Aquest estudi ha estat possible gr\u00e0cies al MINECO, al Programa I3, al ISCII\/FEDER, a AGAUR, a la Fundaci\u00f3 la Marat\u00f3 de Tv3 i al Consell Europeu de Recerca (ERC).<\/p>\n

—<\/p>\n

Article de refer\u00e8ncia:<\/em> <\/strong>Isil Tekeli, Isabelle Aujard, Xavier Trepat, Ludovic Jullien, Angel Raya & Dobryna Zalvidea (2016) Long-term in vivo single-cell lineage tracing of Deep structures using three-photon activation<\/a>. Light: Science & applications, 5, 1-7<\/em><\/p>\n

—<\/p>\n

IBEC als mitjans:<\/em><\/strong><\/p>\n

La Vanguaria, “Logran activar c\u00e9lulas profundas del coraz\u00f3n sin causar da\u00f1o al organismo”
\n<\/a>El Punt Avui, “Activen c\u00e8l\u00b7lules de teixits profunds amb tres fotons”
\n<\/a>Agencia SINC, “Logran rastrear c\u00e9lulas en tejidos profundos”<\/a>
\nEl Economista, “Logran activar c\u00e9lulas profundas del coraz\u00f3n sin casusar da\u00f1o al organismo”
\n<\/a>Noticias de la Ciencia, “Investigadores logran rastrear c\u00e9lulas en tejidos profundos”
\n<\/a>Presspeople, “Investigadores logran rastrear c\u00e9lulas en tejidos profundos”<\/a>
\nCatalunya Vanguardista, “Manipulaci\u00f3n gen\u00e9tica no invasiva”<\/a>
\n<\/a>Ciencia Explora, “Logran rastrear c\u00e9lulas en tejidos profundos”<\/a>
\n <\/p>\n","protected":false},"excerpt":{"rendered":"

Investigadors de l’Institut de Bioenginyeria de Catalunya (IBEC) i els seus col\u00b7laboradors del CMR[B] han desenvolupat una nova t\u00e8cnica basada en la fotoactivaci\u00f3 (activaci\u00f3 per llum), que permet la manipulaci\u00f3 gen\u00e8tica no invasiva in vivo per a l’activaci\u00f3 i rastreig de c\u00e8l\u00b7lules en teixits profunds.<\/strong><\/h3>\n

L’estudi, publicat en la revista Light: Science & Applications<\/em>, del grup editorial Nature<\/em>, revela per primera vegada, que la il\u00b7luminaci\u00f3 amb longituds d’ona m\u00e9s llargues implica una menor dispersi\u00f3 dels fotons que viatgen a trav\u00e9s dels teixits.<\/p>\n","protected":false},"author":4,"featured_media":23247,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[96],"tags":[182,183,184],"post_formats":[],"class_list":["post-23264","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-noticies-de-recerca","tag-fotoactivacion-ca","tag-pez-cebra-ca","tag-rastreo-celular-ca"],"yoast_head":"\nInvestigadors aconsegueixen rastrejar c\u00e8l\u00b7lules en teixits profunds - Institute for Bioengineering of Catalonia<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/ibecbarcelona.eu\/ca\/investigadores-logran-rastrear-celulas-en-tejidos-profundos-3\/\" \/>\n<meta property=\"og:locale\" content=\"ca_ES\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Investigadors aconsegueixen rastrejar c\u00e8l\u00b7lules en teixits profunds - Institute for Bioengineering of Catalonia\" \/>\n<meta property=\"og:description\" content=\"Investigadors de l'Institut de Bioenginyeria de Catalunya (IBEC) i els seus col\u00b7laboradors del CMR[B] han desenvolupat una nova t\u00e8cnica basada en la fotoactivaci\u00f3 (activaci\u00f3 per llum), que permet la manipulaci\u00f3 gen\u00e8tica no invasiva in vivo per a l'activaci\u00f3 i rastreig de c\u00e8l\u00b7lules en teixits profunds. 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