{"id":26677,"date":"2016-12-01T17:21:00","date_gmt":"2016-12-01T16:21:00","guid":{"rendered":"http:\/\/ibecbarcelona.eu\/?p=26677"},"modified":"2016-12-15T12:56:50","modified_gmt":"2016-12-15T11:56:50","slug":"investigadors-de-libec-utilitzen-la-microscopia-efm-per-desvetllar-els-secrets-de-la-supervivencia-de-les-endospores-bacterianes","status":"publish","type":"post","link":"https:\/\/ibecbarcelona.eu\/ca\/investigadors-de-libec-utilitzen-la-microscopia-efm-per-desvetllar-els-secrets-de-la-supervivencia-de-les-endospores-bacterianes\/","title":{"rendered":"Investigadors de l’IBEC utilitzen la microscopia EFM per desvetllar els secrets de la superviv\u00e8ncia de les endospores bacterianes"},"content":{"rendered":"
Imatge: Imatges d’EFM d’una endospora i d’una c\u00e8l\u00b7lula bacteriana en condicions de baixa (a dalt) i alta (a baix) humitat relativa. L’augment del contrast en les imatges produ\u00efdes en condicions d’alta humitat relativa es relaciona amb l’absorci\u00f3 d’humitat de l’endospora i la c\u00e8l\u00b7lula bacteriana.<\/h6>\n

Les endospores, considerades la forma de vida m\u00e9s resistent del planeta, es produeixen a partir de certes c\u00e8l\u00b7lules bacterianes en resposta a la falta de nutrients. Estan compostes per una microc\u00e0psula herm\u00e8tica feta de prote\u00efnes i l\u00edpids que cont\u00e9 el material gen\u00e8tic bacteri\u00e0, i el seu disseny els permet resistir condicions ambientals adverses. <\/p>\n

Les endospores poden romandre en estat latent durant d\u00e8cades en molts entorns, incloent l’aire, el s\u00f2l o la mat\u00e8ria org\u00e0nica. No obstant aix\u00f2, una vegada s’exposen a les condicions ambientals apropiades, aquestes poden germinar de tornada a la seva forma cel\u00b7lular vegetativa en q\u00fcesti\u00f3 de minuts, constituint una estrat\u00e8gia de superviv\u00e8ncia clau per als organismes vius.<\/p>\n

Una de les propietats m\u00e9s destacades de les endospores bacterianes \u00e9s la seva resposta als canvis d’humitat relativa de l’entorn (higroscopicitat), ja que absorbeixen la humitat de l’ambient sense que aix\u00f2 comprometi la seva viabilitat, la qual cosa pot estar relacionada amb la manera com distribueixen internament l’aigua.<\/p>\n

El grup del Gabriel Gomila, Nanoscale Bioelectrical Characterization<\/a>, en col\u00b7laboraci\u00f3 amb l’Antonio Ju\u00e1rez, investigador associat de l’IBEC i professor de la UB, han emprat la Microscopia de Forces Electrost\u00e0tiques (EFM), una variaci\u00f3 de la Microscopia de For\u00e7a At\u00f2mica que permet mesurar les propietats internes d’hidrataci\u00f3 de les endospores en condicions d’alta humitat relativa.<\/p>\n

\u2018Hem pogut observar com l’estructura de les endospores \u00e9s capa\u00e7 de preservar el seu nucli, on es troba l’ADN, fins i tot sotmetent-les a condicions de baixa hidrataci\u00f3, independentment de les condicions relatives d’humitat. Aquesta propietat clau s’afegeix a la resta d’habilitats extraordin\u00e0ries de les endospores\u2019 diu el Gabriel. \u2018Aquesta propietat tamb\u00e9 \u00e9s la base de la seva sorprenent resposta a l’aigua, que ha donat lloc a investigar nous dispositius recol\u00b7lectors d’energia basats en endospores que poden generar energia el\u00e8ctrica a trav\u00e9s de l’evaporaci\u00f3 d’una massa d’aigua, aix\u00ed com dispositius electromec\u00e0nics d’espores amb quantum dots<\/em> de graf\u00e8\u2019.<\/p>\n

Els resultats d’aquesta recerca demostren la precisi\u00f3 i potencial de la t\u00e8cnica EFM per mesurar les propietats higrosc\u00f2piques d’objectes a petita escala, la qual cosa pot ser de gran inter\u00e8s per a d’altres aplicacions nano-higrosc\u00f2piques. Alguns exemples s\u00f3n els materials sensibles a l’aigua per a l’emmagatzematge d’energia, els processos biol\u00f2gics dependents de la humitat, com la producci\u00f3 de micotoxines (una de les majors amenaces per a la seguretat aliment\u00e0ria) o els aerosols que emprin nanopart\u00edcules rellevants per a les ci\u00e8ncies atmosf\u00e8riques.
\n—–<\/p>\n

Paper: <\/em><\/strong>Marc Van Der Hofstadt, Rene Fabregas, Ruben Millan-Solsona, Antonio Juarez, Laura Fumagalli & Gabriel Gomila (2016). Internal Hydration Properties of Single Bacterial Endospores Probed by Electrostatic Force Microscopy.<\/a> ACS Nano<\/em>, 10.1021\/acsnano.6b06578<\/p>\n

Partially supported by the FP7-funded Nanomicrowave<\/a> project (FP7\/People-2012-ITN 317116.EU).<\/em><\/p>\n","protected":false},"excerpt":{"rendered":"

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