{"id":8364,"date":"2014-07-25T07:06:52","date_gmt":"2014-07-25T07:06:52","guid":{"rendered":"http:\/\/ibecbarcelona.eu\/new\/aprofundir-en-el-funcionament-intern-de-les-cellules\/"},"modified":"2015-02-05T10:09:10","modified_gmt":"2015-02-05T10:09:10","slug":"aprofundir-en-el-funcionament-intern-de-les-cellules","status":"publish","type":"post","link":"https:\/\/ibecbarcelona.eu\/ca\/aprofundir-en-el-funcionament-intern-de-les-cellules\/","title":{"rendered":"Aprofundir en el funcionament intern de les c\u00e8l\u2022lules"},"content":{"rendered":"<h6 class=\"caption\" style=\"width: 230px; float: left; padding: 0px 10px 10px 0px;\">Imatge: Una neurona hipocampal fotosensibilitzada amb els nous compostos (esquerra), es pot estimular amb un l\u00e0ser d\u2019impulsos de 900nm per produir una r\u00e0fega de potencials d\u2019acci\u00f3 que es pot mesurar amb la t\u00e8cnica de patch clamp (dreta)<\/h6>\n<p>El grup de Nanosondes i Nanocommutadors  de l\u2019IBEC i els seus col\u2022laboradors a Espanya i als EUA han publicat un article cient\u00edfic a la revista JACS que planteja una nova manera d\u2019utilitzar les prote\u00efnes regulades per la llum. Les prote\u00efnes \u2018commutables\u2019, per control remot, poden activar o inhibir les funcions bioqu\u00edmiques espec\u00edfiques en les c\u00e8l\u2022lules vives, el que permet als investigadors configurar i provar una multitud de situacions dins la c\u00e8l\u2022lula \u2013 per activar o desactivar els processos a un nivell molt m\u00e9s profund en el teixit  i amb major resoluci\u00f3 en 3D.<\/p>\n<p>\u201cLes biomol\u00e8cules fotocommutables s\u2019utilitzen per manipular amb precisi\u00f3 els processos bioqu\u00edmics cel\u2022lulars per\u00f2, fins ara, ning\u00fa ha explorat un m\u00e8tode conegut com a microsc\u00f2pia d\u2019excitaci\u00f3 de dos fotons \u2013 que ofereix una penetraci\u00f3 m\u00e9s profunda del teixit, amb un volum d\u2019excitaci\u00f3 m\u00e9s petit, i redueix la fototoxicitat i la fotodestrucci\u00f3 \u2013 en relaci\u00f3 a aquests interruptors\u201d, explica Pau Gorostiza, investigador principal del grup de Nanosondes i Nanocommutadors del IBEC i Professor d\u2019Investigaci\u00f3 ICREA. \u201cAra, els interruptors de prote\u00efnes existents tamb\u00e9 es poden utilitzar amb excitaci\u00f3 multi-fot\u00f3 amb llum infraroja propera, proporcionant resoluci\u00f3 subcel\u2022lular.\u201d<\/p>\n<p>Els investigadors han ajustat la resposta a la llum de prote\u00efnes fotocommutables existents, per  adaptar-les a la tecnologia d\u2019estimulaci\u00f3 de dos fotons. D\u2019aquesta manera han estat capa\u00e7os d\u2019obtenir el control a nivell unicel\u2022lular i subcel\u2022lular sense alterar les propietats funcionals normals de les prote\u00efnes, i amb molta m\u00e9s precisi\u00f3.<\/p>\n<p>Aquest descobriment constitueix un aven\u00e7 important en l\u2019optogen\u00e8tica i l\u2019optofarmacologia per controlar i visualitzar l\u2019activitat de les neurones, amb la finalitat de poder tra\u00e7ar un mapa dels circuits neuronals.<\/p>\n<p>&#8212;<\/p>\n<p><strong>Article de refer\u00e8ncia:<\/strong> Izquierdo-Serra M, Gasc\u00f3n-Moya M, Hirtz JJ, Pittolo S, Poskanzer KE, Ferrer E, Alib\u00e9s R, Busqu\u00e9 F, Yuste R, Hernando J, Gorostiza P. (2014). <a href=\"http:\/\/pubs.acs.org\/doi\/abs\/10.1021\/ja5026326\" target=\"_blank\">Two-Photon Neuronal and Astrocytic Stimulation with Azobenzene-Based Photoswitches<\/a>. <em>J Am Chem Soc<\/em>. 2014 Jun 18;136(24):8693-701<\/p>\n<p>\u00a0<\/p>\n<p>\u00a0<\/p>\n","protected":false},"excerpt":{"rendered":"<h3><img decoding=\"async\" class=\"left size-full wp-image-8360 lazyload\" data-src=\"https:\/\/ibecbarcelona.eu\/wp-content\/uploads\/2014\/07\/neuron_340x210.jpg\" border=\"0\" width=\"340\" height=\"210\" data-srcset=\"https:\/\/ibecbarcelona.eu\/wp-content\/uploads\/2014\/07\/neuron_340x210.jpg 340w, https:\/\/ibecbarcelona.eu\/wp-content\/uploads\/2014\/07\/neuron_340x210-300x185.jpg 300w, https:\/\/ibecbarcelona.eu\/wp-content\/uploads\/2014\/07\/neuron_340x210-242x150.jpg 242w, https:\/\/ibecbarcelona.eu\/wp-content\/uploads\/2014\/07\/neuron_340x210-150x92.jpg 150w\" data-sizes=\"(max-width: 340px) 100vw, 340px\" src=\"data:image\/svg+xml;base64,PHN2ZyB3aWR0aD0iMSIgaGVpZ2h0PSIxIiB4bWxucz0iaHR0cDovL3d3dy53My5vcmcvMjAwMC9zdmciPjwvc3ZnPg==\" style=\"--smush-placeholder-width: 340px; --smush-placeholder-aspect-ratio: 340\/210;\" \/>La recerca duta a terme a l\u2019IBEC ha obert el cam\u00ed cap a les noves aplicacions per controlar l\u2019activaci\u00f3 de les neurones o altres parts funcionals de les c\u00e8l\u2022lules.<\/h3>\n<p>El somni de controlar amb precisi\u00f3 i de forma remota tots els aspectes del funcionament intern cel\u2022lular en el teixit ofereix la promesa de descobrir els mecanismes moleculars dels processos cel\u2022lulars complexes. Cosa que al seu torn pot donar lloc a grans aven\u00e7os en la nostra comprensi\u00f3 del que succeeix quan les coses van malament \u2013 per exemple, com i quan les malalties neurodegeneratives poden desenvolupar-se.<\/p>\n","protected":false},"author":4,"featured_media":8362,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[96],"tags":[],"post_formats":[],"class_list":["post-8364","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-noticies-de-recerca"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.4 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Aprofundir en el funcionament intern de les c\u00e8l\u2022lules - Institute for Bioengineering of Catalonia<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/ibecbarcelona.eu\/ca\/aprofundir-en-el-funcionament-intern-de-les-cellules\/\" \/>\n<meta property=\"og:locale\" content=\"ca_ES\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Aprofundir en el funcionament intern de les c\u00e8l\u2022lules - Institute for Bioengineering of Catalonia\" \/>\n<meta property=\"og:description\" content=\"La recerca duta a terme a l\u2019IBEC ha obert el cam\u00ed cap a les noves aplicacions per controlar l\u2019activaci\u00f3 de les neurones o altres parts funcionals de les c\u00e8l\u2022lules. El somni de controlar amb precisi\u00f3 i de forma remota tots els aspectes del funcionament intern cel\u2022lular en el teixit ofereix la promesa de descobrir els mecanismes moleculars dels processos cel\u2022lulars complexes. 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