{"id":8441,"date":"2014-09-17T07:43:02","date_gmt":"2014-09-17T07:43:02","guid":{"rendered":"http:\/\/ibecbarcelona.eu\/new\/la-collaboracio-interna-al-ibec-te-exit-en-la-mesura-de-la-resposta-de-les-cellules-bacterianes-als-camps-electrics\/"},"modified":"2015-09-28T12:35:39","modified_gmt":"2015-09-28T12:35:39","slug":"la-collaboracio-interna-al-ibec-te-exit-en-la-mesura-de-la-resposta-de-les-cellules-bacterianes-als-camps-electrics","status":"publish","type":"post","link":"https:\/\/ibecbarcelona.eu\/ca\/la-collaboracio-interna-al-ibec-te-exit-en-la-mesura-de-la-resposta-de-les-cellules-bacterianes-als-camps-electrics\/","title":{"rendered":"La col\u2022laboraci\u00f3 interna al IBEC t\u00e9 \u00e8xit en la mesura de la resposta de les c\u00e8l\u2022lules bacterianes als camps el\u00e8ctrics"},"content":{"rendered":"

Ser capa\u00e7 de mesurar la polaritat el\u00e8ctrica \u2013 que indica com es reacciona a un camp el\u00e8ctric extern \u2013 d\u2019una \u00fanica c\u00e8l\u2022lula bacteriana pot donar llom sobre els components bioqu\u00edmics del bacteri, aix\u00ed com sobre la seva estructura interna, de manera que s\u2019obririen noves possibilitats pels estudis anal\u00edtics i noves exploracions per avaluar les seves propietats biol\u00f2giques cr\u00edtiques com l\u2019adher\u00e8ncia, la virul\u00e8ncia o la viabilitat <\/p>\n

Els investigadors han utilitzat al seu estudi el microscopi de for\u00e7a electrost\u00e0tica quantitativa (EFM, de l\u2019angl\u00e8s). Es tracta d\u2019una t\u00e8cnica que ha estat recentment desenvolupada i aplicada amb \u00e8xit per mesurar les propietats el\u00e8ctriques de nano-objectes 3D com les nanopart\u00edcules o els virus. El EFM detecta les propietats el\u00e8ctriques de la c\u00e8l\u2022lula bacteriana, incloent tota la seva regi\u00f3 citoplasm\u00e0tica, i aix\u00ed \u00e9s capa\u00e7 de proporcionar una gran quantitat d\u2019informaci\u00f3 sobre la resposta el\u00e8ctrica d\u2019un bacteri.<\/p>\n

D\u2019aquesta manera, els investigadors han quantificat la resposta de la polaritzaci\u00f3 el\u00e8ctrica de quatre tipus de bacteris \u2013 Lactobacilus sakei<\/em>, Salmonella Typhimurium<\/em>, Escherchia coli<\/em> i Listeria innocua<\/em>, els quals tenen o b\u00e9 rellev\u00e0ncia cl\u00ednica o b\u00e9 industrial. Els resultats han mostrat difer\u00e8ncies importants entre bact\u00e8ries Gram-negatives i Gram-positives.<\/p>\n

Estudis el\u00e8ctrics anteriors \u2013 que fins ara nom\u00e9s s\u2019han pogut dur a terme en poblacions bacterianes que involucren a milions de c\u00e8l\u2022lules bacterianes, i no en les c\u00e8l\u2022lules individuals \u2013 han perm\u00e8s als investigadors poder detectar bacteris en un ambient, contar-los i diferenciar-los, determinar la seva viabilitat, distingir mutants fins i tot entre els genotips molt similars, i separar-los de les altres c\u00e8l\u2022lules. El fet que aquesta nova t\u00e8cnica permeti la mesura de les propietats el\u00e8ctriques de les c\u00e8l\u2022lules individuals sense la necessitat de la separaci\u00f3 permet quantificar amb precisi\u00f3 l\u2019heterogene\u00eftat dins d\u2019una mateixa poblaci\u00f3.<\/p>\n

—<\/p>\n

Article de Refer\u00e8ncia: <\/strong>Daniel Esteban-Ferrer, Martin A. Edwards, Laura Fumagalli, Antonio Ju\u00e1rez, and Gabriel Gomila (2014). Electric Polarization Properties of Single Bacteria Measured with Electrostatic Force Microscopy.<\/a> ACS Nano,<\/em> 8 (10), pp 9843\u20139849<\/p>\n","protected":false},"excerpt":{"rendered":"

Dos grups que treballen junts al IBEC demostren el potencial dels estudis el\u00e8ctrics de les c\u00e8l\u2022lules bacterianes individuals en un article publicat a ACS Nano.<\/h3>\n

El grup de Gabriel Gomila, Caracteritzaci\u00f3 Bioel\u00e8ctrica a la Nanoescala, i el d\u2019Antoni Ju\u00e1rez, Biotecnologia Microbiana i Interacci\u00f3 Hoste-patogen, combinen els seus coneixements en les mesures de microsc\u00f2pia electr\u00f2nica i bacteris per trobar una manera d0estudiar la resposta a camps el\u00e8ctrics externs de nom\u00e9s una \u00fanica c\u00e8l\u2022lula bacteriana.<\/p>\n","protected":false},"author":4,"featured_media":8439,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[96],"tags":[],"post_formats":[],"class_list":["post-8441","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-noticies-de-recerca"],"yoast_head":"\nLa col\u2022laboraci\u00f3 interna al IBEC t\u00e9 \u00e8xit en la mesura de la resposta de les c\u00e8l\u2022lules bacterianes als camps el\u00e8ctrics - Institute for Bioengineering of Catalonia<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/ibecbarcelona.eu\/ca\/la-collaboracio-interna-al-ibec-te-exit-en-la-mesura-de-la-resposta-de-les-cellules-bacterianes-als-camps-electrics\/\" \/>\n<meta property=\"og:locale\" content=\"ca_ES\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"La col\u2022laboraci\u00f3 interna al IBEC t\u00e9 \u00e8xit en la mesura de la resposta de les c\u00e8l\u2022lules bacterianes als camps el\u00e8ctrics - Institute for Bioengineering of Catalonia\" \/>\n<meta property=\"og:description\" content=\"Dos grups que treballen junts al IBEC demostren el potencial dels estudis el\u00e8ctrics de les c\u00e8l\u2022lules bacterianes individuals en un article publicat a ACS Nano. 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