{"id":70281,"date":"2020-01-13T16:57:52","date_gmt":"2020-01-13T15:57:52","guid":{"rendered":"http:\/\/ibecbarcelona.eu\/?post_type=tribe_events&p=70281&lang=ca"},"modified":"2020-01-13T16:57:52","modified_gmt":"2020-01-13T15:57:52","slug":"phd-thesis-defence-aida-garrido","status":"publish","type":"tribe_events","link":"https:\/\/ibecbarcelona.eu\/ca\/event\/phd-thesis-defence-aida-garrido\/","title":{"rendered":"PhD Thesis Defence: Aida Garrido"},"content":{"rendered":"

Control \u00f2ptic de receptors end\u00f2gens i excitabilitat cel\u00b7lular amb llum<\/h2>\n

Aida Garrido, Nanoprobes and nanoswitches group<\/em><\/p>\n

L’estudi electrofisiol\u00f2gic i d\u2019imatge de l\u2019activitat neuronal en temps real requereix una gran resoluci\u00f3 espacial i temporal. Ambd\u00f3s trets es poden aconseguir amb la precisi\u00f3, velocitat i control remot que permet la llum. En aquesta tesi es descriu la caracteritzaci\u00f3 de nous fotocommutadors dirigits a fotosensibilitzar receptors end\u00f2gens de glutamat i estrat\u00e8gies per la millora de les seves caracter\u00edstiques \u00f2ptiques.
\nEls primers fotoconmutadors descrits capa\u00e7os de controlar amb llum l\u2019activitat de canals end\u00f2gens de glutamat son efica\u00e7os en altres aproximacions experimentals com ara en la inducci\u00f3 amb llum de potencials d\u2019acci\u00f3 en neurones en cultiu, en cultius organot\u00edpics d\u2019hipocamp de rata o in vivo en larves de Xenopus electroporades, a nivell d\u2019un grup neuronal, d\u2019una \u00fanica neurona o d\u2019una \u00fanica espina sin\u00e0ptica. Modificant la part reactiva del fotocommutador, som podem controlar espacialment la conjugaci\u00f3 mitjan\u00e7ant patrons d\u2019il\u00b7luminaci\u00f3.
\nCanviant l\u2019estructura qu\u00edmica s\u2019aconsegueix un fotocommutador actiu en el rang visible de llum i alhora extremadament r\u00e0pid. Fet que el fa ideal pel control de sinapsis ultra r\u00e0pides com ara les encarregades de codificar el so en la c\u00f2clea. El disseny racional d\u2019altres modificacions qu\u00edmiques en fotocommutadors de receptors de glutamat (LiGluRs) obre les portes al disseny de mol\u00e8cules sensibles a l\u2019activaci\u00f3 per 2 fotons. L\u2019estimulaci\u00f3 de 2 fotons amb l\u00e0sers polsats de llum infraroja comporta importants avantatges com ara l\u2019augment de la capacitat de penetraci\u00f3 en teixit i una disminuci\u00f3 de possibles danys degut a una sobreexposici\u00f3 a la llum. Caracter\u00edstiques molt \u00fatils per estudis in vivo. Modificacions qu\u00edmiques dels fotocommutadors comporten altres modificacions en les seves caracter\u00edstiques fotodin\u00e0miques. Per aquest motiu, hem desenvolupat una estrat\u00e8gia de conjugaci\u00f3 de fluor\u00f2fors col\u00b7lectors de llum que mitjan\u00e7ant RET transfereixen la seva energia al crom\u00f2for (fotocommutador), modificant-ne les caracter\u00edstiques espectrals.
\nEs demostra que crom\u00f2fors no conjugables permeten controlar l\u2019activitat neuronal. Amb diversos avantatges respecte l\u2019azobenz\u00e8 cl\u00e0ssic: els azobenzens c\u00edclics reverteix l\u2019estabilitat i en conseq\u00fc\u00e8ncia l\u2019activitat de la mol\u00e8cula; i els estilbens permeten l\u2019activaci\u00f3 irreversible per\u00f2 no foto-destructiva de la mol\u00e8cula original, evitant aix\u00ed la creaci\u00f3 de foto-productes.
\nEn conclusi\u00f3, en aquesta tesi es presenten noves mol\u00e8cules i estrat\u00e8gies de disseny per al control de la neurotransmissi\u00f3 sense necessitat de modificacions gen\u00e8tiques.<\/p>\n","protected":false},"excerpt":{"rendered":"

Control \u00f2ptic de receptors end\u00f2gens i excitabilitat cel\u00b7lular amb llum Aida Garrido, Nanoprobes and nanoswitches group L’estudi electrofisiol\u00f2gic i d\u2019imatge de l\u2019activitat neuronal en temps real requereix una gran resoluci\u00f3 … Read more<\/a><\/p>\n","protected":false},"author":4,"featured_media":0,"template":"","meta":{"_tribe_events_status":"","_tribe_events_status_reason":"","footnotes":""},"tags":[],"tribe_events_cat":[367],"class_list":["post-70281","tribe_events","type-tribe_events","status-publish","hentry","tribe_events_cat-phd-thesis-defence-ca","cat_phd-thesis-defence-ca"],"yoast_head":"\nPhD Thesis Defence: Aida Garrido - Institute for Bioengineering of Catalonia<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/ibecbarcelona.eu\/ca\/event\/phd-thesis-defence-aida-garrido\/?lang=esbactinfcalendarcalendarcalendarcalendar\/\" \/>\n<meta property=\"og:locale\" content=\"ca_ES\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"PhD Thesis Defence: Aida Garrido - Institute for Bioengineering of Catalonia\" \/>\n<meta property=\"og:description\" content=\"Control \u00f2ptic de receptors end\u00f2gens i excitabilitat cel\u00b7lular amb llum Aida Garrido, Nanoprobes and nanoswitches group L’estudi electrofisiol\u00f2gic i d\u2019imatge de l\u2019activitat neuronal en temps real requereix una gran resoluci\u00f3 ... 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