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DTSTART;TZID=Europe/Madrid:20150508T100000
DTEND;TZID=Europe/Madrid:20150508T110000
DTSTAMP:20260418T222947
CREATED:20150311T074930Z
LAST-MODIFIED:20150311T074930Z
UID:95839-1431079200-1431082800@ibecbarcelona.eu
SUMMARY:IBEC Seminar: J. Miguel Rubi
DESCRIPTION:Somatic exocytosis of serotonin mediated by molecular motors\n \nJ. Miguel Rubi\, Departament de Fisica Fonamental\, Universitat de Barcelona\nWe quantified somatic exocytosis of serotonin in Retzius neurons and explored the possible contribution of molecular motors and the cytoskeleton on the mobilization of vesicles induced by stimulation with trains of electrical impulses. Secretion was quantified from the increase of fluorescence of FM1-43 in response to sequences of 20 mV trains of 10 impulses at 2 min intervals\, produced by intracellular current injection. Somatic secretion was also evoked by a pulse of 10 mM caffeine applied to the bathing solution. Stimulation of neurons produced a gradual increase in FM1-43 fluorescence for over the next five minutes. The kinetics and latencies of these increases varied from one neuron to another but usually maintaining a sigmoidal shape in one or two steps. Neurons stimulation in the presence of colchicine to uncouple microtubules\, failed to evoke fluorescence increases\, thus suggesting that vesicle mobilization depended on tubulin-based motor. The kinetics of the fluorescence increases of individual neurons was accounted for by using a model based on a diffusion equation in the presence of external forces\, consistent with the contribution of molecular motors to the mobilization of the vesicle clusters towards the membrane in response to electrical activity. Our data show that somatic serotonin secretion in Retzius neurons depends on a motor-based cytoskeletal mobilization of vesicles induced by electrical activity.
URL:https://ibecbarcelona.eu/event/15400-2/
CATEGORIES:IBEC Seminar
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Europe/Madrid:20150508T100000
DTEND;TZID=Europe/Madrid:20150508T110000
DTSTAMP:20260418T222947
CREATED:20150311T074930Z
LAST-MODIFIED:20150505T144616Z
UID:15400-1431079200-1431082800@ibecbarcelona.eu
SUMMARY:IBEC Seminar: J. Miguel Rubi
DESCRIPTION:Somatic exocytosis of serotonin mediated by molecular motors\n \nJ. Miguel Rubi\, Departament de Fisica Fonamental\, Universitat de Barcelona\nWe quantified somatic exocytosis of serotonin in Retzius neurons and explored the possible contribution of molecular motors and the cytoskeleton on the mobilization of vesicles induced by stimulation with trains of electrical impulses. Secretion was quantified from the increase of fluorescence of FM1-43 in response to sequences of 20 mV trains of 10 impulses at 2 min intervals\, produced by intracellular current injection. Somatic secretion was also evoked by a pulse of 10 mM caffeine applied to the bathing solution. Stimulation of neurons produced a gradual increase in FM1-43 fluorescence for over the next five minutes. The kinetics and latencies of these increases varied from one neuron to another but usually maintaining a sigmoidal shape in one or two steps. Neurons stimulation in the presence of colchicine to uncouple microtubules\, failed to evoke fluorescence increases\, thus suggesting that vesicle mobilization depended on tubulin-based motor. The kinetics of the fluorescence increases of individual neurons was accounted for by using a model based on a diffusion equation in the presence of external forces\, consistent with the contribution of molecular motors to the mobilization of the vesicle clusters towards the membrane in response to electrical activity. Our data show that somatic serotonin secretion in Retzius neurons depends on a motor-based cytoskeletal mobilization of vesicles induced by electrical activity.
URL:https://ibecbarcelona.eu/event/15400/
CATEGORIES:IBEC Seminar
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Europe/Madrid:20150522T100000
DTEND;TZID=Europe/Madrid:20150522T110000
DTSTAMP:20260418T222947
CREATED:20150408T132341Z
LAST-MODIFIED:20150408T132405Z
UID:15816-1432288800-1432292400@ibecbarcelona.eu
SUMMARY:IBEC Seminar: Thomas Graf
DESCRIPTION:Mechanisms of transcription factor induced transdifferentiation and reprogramming to pluripotency\n \nThomas Graf\, Gene Regulation\, Stem Cells and Differentiation Programme\, CRG / Pompeu Fabra University\nWork by many laboratories has shown that even fully differentiated cells are remarkably plastic and can be reprogrammed into alternative fates. This discovery has revolutionized our understanding of how cell decide what to become and has major implications for the modeling and therapy of diseases that affect the production of differentiated cells. \nOur earlier work has shown that the myeloid transcription factor C/EBPa induces B cells to transdifferentiate into macrophages at high efficiencies. We have now found that the process forces the intersection of two enhancer pathways that become activated during normal hematopoietic differentiation. \nRecently we reported that the transient expression of C/EBPa in B cells\, followed by expression of the pluripotency factors Oct4\, Sox2\, Klf4 and c-Myc (OSKM)\, poises the cells for very rapid and highly efficient reprogramming into induced pluripotent stem cells. Our findings have removed a major obstacle in studying cell reprogramming and permitted us to investigate how C/EBPa leads to the almost immediate accessibility of pluripotency genes to binding by Oct4. Our new data provide unprecedented insights into the earliest events leading to activation of the pluripotency gene regulatory network\, resulting in somatic cell reprogramming.
URL:https://ibecbarcelona.eu/event/ibec-seminar-thomas-graf/
CATEGORIES:IBEC Seminar
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Europe/Madrid:20150522T100000
DTEND;TZID=Europe/Madrid:20150522T110000
DTSTAMP:20260418T222947
CREATED:20150408T132341Z
LAST-MODIFIED:20150408T132341Z
UID:95842-1432288800-1432292400@ibecbarcelona.eu
SUMMARY:IBEC Seminar: Thomas Graf
DESCRIPTION:Mechanisms of transcription factor induced transdifferentiation and reprogramming to pluripotency\n \nThomas Graf\, Gene Regulation\, Stem Cells and Differentiation Programme\, CRG / Pompeu Fabra University\nWork by many laboratories has shown that even fully differentiated cells are remarkably plastic and can be reprogrammed into alternative fates. This discovery has revolutionized our understanding of how cell decide what to become and has major implications for the modeling and therapy of diseases that affect the production of differentiated cells. \nOur earlier work has shown that the myeloid transcription factor C/EBPa induces B cells to transdifferentiate into macrophages at high efficiencies. We have now found that the process forces the intersection of two enhancer pathways that become activated during normal hematopoietic differentiation. \nRecently we reported that the transient expression of C/EBPa in B cells\, followed by expression of the pluripotency factors Oct4\, Sox2\, Klf4 and c-Myc (OSKM)\, poises the cells for very rapid and highly efficient reprogramming into induced pluripotent stem cells. Our findings have removed a major obstacle in studying cell reprogramming and permitted us to investigate how C/EBPa leads to the almost immediate accessibility of pluripotency genes to binding by Oct4. Our new data provide unprecedented insights into the earliest events leading to activation of the pluripotency gene regulatory network\, resulting in somatic cell reprogramming.
URL:https://ibecbarcelona.eu/event/ibec-seminar-thomas-graf-2/
CATEGORIES:IBEC Seminar
END:VEVENT
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