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X-ORIGINAL-URL:https://ibecbarcelona.eu
X-WR-CALDESC:Events for Institute for Bioengineering of Catalonia
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DTSTART:20150101T000000
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BEGIN:VEVENT
DTSTART;TZID=UTC:20160311T100000
DTEND;TZID=UTC:20160311T110000
DTSTAMP:20260416T225826
CREATED:20160229T091313Z
LAST-MODIFIED:20160229T091313Z
UID:95898-1457690400-1457694000@ibecbarcelona.eu
SUMMARY:IBEC Seminar: Samuel Ojosnegros
DESCRIPTION:Imaging Eph/ephrin cell-cell communication through Enhanced Number and Brightness: a novel method for the study of protein aggregation\nDr. Samuel Ojosnegros\, Centre de Medicina Regenerativa de Barcelona (CMRB)\nProteins constantly interact with each other assembling a variety of molecular species\, including small oligomers\, fibers of dynamic polymerization or high-order clusters\, among others. However\, current methods discriminate poorly the stoichiometry of protein interactions in living cells. To overcome these impediments we developed an enhanced version of Number & Brightness (eN&B)\, a powerful live imaging technique with capability to investigate multiple domains of information.  eN&B determines the distribution of protein aggregates present inside every pixel in an image during time-lapse movies. It can reach unprecedented level of space and time resolution compared to FRET-based or FCS-based methods. When studying EphB2 receptor activation\, the eN&B allowed us to resolve simultaneously the aggregation state of 40 different oligomers in 3 dimensions (x\, y\, t) and pixel depth. We found that Eph oligomerization follows a sophisticated dynamics consistent with a nucleated polymerization process\, where activation and clustering are decoupled processes. This decoupling endows the receptor with high sensitivity for low ligand concentrations while at the same time allows to provide a proportional response to a wide range of ligand concentrations. In summary\, I will present a novel imaging tool with capability to determine the stoichiometry of receptor-ligand interactions and potentially\, many other instances or protein aggregation.
URL:https://ibecbarcelona.eu/event/ibec-seminar-samuel-ojosnegros-2/
CATEGORIES:IBEC Seminar
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=UTC:20160311T100000
DTEND;TZID=UTC:20160311T110000
DTSTAMP:20260416T225826
CREATED:20160229T091313Z
LAST-MODIFIED:20160229T091313Z
UID:21728-1457690400-1457694000@ibecbarcelona.eu
SUMMARY:IBEC Seminar: Samuel Ojosnegros
DESCRIPTION:Imaging Eph/ephrin cell-cell communication through Enhanced Number and Brightness: a novel method for the study of protein aggregation\nDr. Samuel Ojosnegros\, Centre de Medicina Regenerativa de Barcelona (CMRB)\nProteins constantly interact with each other assembling a variety of molecular species\, including small oligomers\, fibers of dynamic polymerization or high-order clusters\, among others. However\, current methods discriminate poorly the stoichiometry of protein interactions in living cells. To overcome these impediments we developed an enhanced version of Number & Brightness (eN&B)\, a powerful live imaging technique with capability to investigate multiple domains of information.  eN&B determines the distribution of protein aggregates present inside every pixel in an image during time-lapse movies. It can reach unprecedented level of space and time resolution compared to FRET-based or FCS-based methods. When studying EphB2 receptor activation\, the eN&B allowed us to resolve simultaneously the aggregation state of 40 different oligomers in 3 dimensions (x\, y\, t) and pixel depth. We found that Eph oligomerization follows a sophisticated dynamics consistent with a nucleated polymerization process\, where activation and clustering are decoupled processes. This decoupling endows the receptor with high sensitivity for low ligand concentrations while at the same time allows to provide a proportional response to a wide range of ligand concentrations. In summary\, I will present a novel imaging tool with capability to determine the stoichiometry of receptor-ligand interactions and potentially\, many other instances or protein aggregation.
URL:https://ibecbarcelona.eu/event/ibec-seminar-samuel-ojosnegros/
CATEGORIES:IBEC Seminar
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=UTC:20160318T100000
DTEND;TZID=UTC:20160318T110000
DTSTAMP:20260416T225826
CREATED:20160229T090903Z
LAST-MODIFIED:20160229T090903Z
UID:95897-1458295200-1458298800@ibecbarcelona.eu
SUMMARY:IBEC Seminar: Mateu Pla
DESCRIPTION:What can we do with our 3D Bioprinter?\nDr. Mateu Pla\, Coordinator of Nanotechnology Platform\nA 3D bioprinter (3D-Discovery from RegenHU ) has recently arrived at IBEC\, and the first training courses have already started. \n3D Bioprinters allow us to generate 3D scaffolds combining biomaterials and cell-laden hydrogels. The applications of this 3D constructs spans from 3D cellular models for drug screening (i.e. dermis)\, scaffolds for regenerative medicine or\, at a more advanced stage\, organ-printing. \nDuring this presentation\, an introduction to bioprinting and the current and future capabilities of IBEC’s 3D bioprinter will be presented.
URL:https://ibecbarcelona.eu/event/ibec-seminar-mateu-pla-2/
CATEGORIES:IBEC Seminar
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=UTC:20160318T100000
DTEND;TZID=UTC:20160318T110000
DTSTAMP:20260416T225826
CREATED:20160229T090903Z
LAST-MODIFIED:20160311T103315Z
UID:21726-1458295200-1458298800@ibecbarcelona.eu
SUMMARY:IBEC Seminar: Mateu Pla
DESCRIPTION:What can we do with our 3D Bioprinter?\nDr. Mateu Pla\, Coordinator of Nanotechnology Platform\nA 3D bioprinter (3D-Discovery from RegenHU ) has recently arrived at IBEC\, and the first training courses have already started. \n3D Bioprinters allow us to generate 3D scaffolds combining biomaterials and cell-laden hydrogels. The applications of this 3D constructs spans from 3D cellular models for drug screening (i.e. dermis)\, scaffolds for regenerative medicine or\, at a more advanced stage\, organ-printing. \nDuring this presentation\, an introduction to bioprinting and the current and future capabilities of IBEC’s 3D bioprinter will be presented.
URL:https://ibecbarcelona.eu/event/ibec-seminar-mateu-pla/
CATEGORIES:IBEC Seminar
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