Institute for Bioengineering of Catalonia

Ibec Seminar. Anne de Poulpiquet
2025-12-12 10:00 - 11:00 (Europe/Madrid) | Baobab room, Floor 11, Tower 1
CHARACTERIZATION OF ENZYMATIC BIOELECTRODES BY IN SITU FLUORESCENCE MICROSCOPY
A. de Poulpiquet,1 A. Guessab, 1 H. M. Man,1 I. Mazurenko,1 L. Bouffier,2 E. Lojou1
1Aix-Marseille Univ., CNRS, Bioenergetics and Protein Engineering, UMR 7281, Marseille
2 Institute of Molecular Sciences, UMR CNRS 5255, Univ. Bordeaux, ENSMAC, Pessac
adepoulpiquet@imm.cnrs.fr
Redox enzymes present remarkable catalytic properties (exceptional selectivity, high kinetic constant, low overvoltage, etc.) which are particularly interesting for bio-electrochemical devices (biosensors, biofuel cells, bioreactors). In the latter, they are immobilized at the surface of an electrode to enable electron transfer. Using three-dimensional (3D) electrodes improves the performance of the devices (sensitivity, current densities). However, enzymatic catalysis is very sensitive to the local environment (pH, temperature, ionic strength, concentration of substrates, products or inhibitors, etc.) whose composition, in the case of interfacial reactions, can differ from the bulk of the solution. These disparities are exacerbated when the enzymes are confined in the pores of 3D electrodes, due to the complexity of the associated mass transport. However, electrochemistry only provides indirect information on the environment of the electrode. Therefore, there is a major interest in coupling electrochemical techniques to other methods for collecting simultaneously spatial information.1-3 Precious information about mass transport and reactivity can be obtained by investigating the concentration profiles of the different species near the electrode surface, or in the volume of a porous electrode. We show that in situ fluorescence confocal laser-scanning microscopy (FCLSM) coupled with electrochemistry enables investigation of redox enzyme reactivity involving the indirect generation of fluorogenic species.4, 5 One of the most interesting features of FCLSM is the possibility to reconstruct 3D concentration profiles. Recording fluorescence in the volume adjacent to the electrode under potential control thus enables rebuilding the diffusion layer.2-5 We show that the method can be implemented to characterize electro-enzymatic catalysis at various planar and structured 3D electrodes.4, 5 For example, enzymatic O2 reduction involves proton transfers, which was evidenced via the fluorescence change of strongly pH-dependent fluorophores. Local pH changes in the electrode plane were measured during O2 reduction catalyzed by an immobilized bilirubin oxidase. Moreover, proton gradients generated during the enzymatic electrode reaction were imaged and their expansion under various experimental conditions were determined. Finally, the method enabled direct imaging of the evolution of confined environments in porous 3D electrodes such as gas-diffusion layers during electro(enzymatic) catalysis.

Ibec Seminar. Albert Folch
2025-12-18 12:30 - 14:00
“MICROFLUIDIC AND ROBOTIC PLATFORMS FOR TME-FRIENDLY CANCER DRUG EVALUATIONS”
Albert Folch, Professor University of Washington, Bioengineering Dept.
There is a lack of confidence in present in vitro disease models and drug efficacy tests, as they do not properly recapitulate the dynamic physiology and pathophysiology of the human organism. This challenge is particularly acute in oncology: present tools to study drug responses fail to faithfully mimic the patient’s tumor microenvironment (TME) and thus have not kept up with tumor biology and drug testing needs. As a measure of this problem, on average less than 4% of oncology drugs in clinical trials end up being FDA-approved, a dismal approval rate that has dire social repercussions such as high cancer drug prices and difficult accessibility. We have developed a suite of microfluidic platforms that address this problem by multiplexing the delivery of drugs to intact-TME human biopsies, altogether bypassing animal testing. We have developed and patented a microdissection methodology that allows for producing large numbers of cuboidal micro-tissues (“cuboids”) from a single tumor biopsy. We have been able to trap cuboids in arrays of microfluidic traps in a 96-well platform and we have developed very high-throughput automated robotic placement of mouse and human cuboids in 384-well plates. With these approaches, it will soon be possible to bypass animal testing and perform direct testing of drugs using only human tumors. Since these new-generation tests preserve the TME intact, we envision that they will minimize FDA failure rates and will contribute to alleviate the cost of cancer drugs. In this talk, I will also cover innovative 3D printing approaches of general applicability to the fabrication of complex biomicrofluidic systems such as organs-on-chips.
Albert Folch’s lab works at the interface between microfluidics and cancer. He received both his BSc (1989) and PhD (1994) in Physics from the University of Barcelona (UB), Spain, in 1989. During his Ph.D. he was a visiting scientist from 1990–91 at the Lawrence Berkeley Lab working on AFM/STM under Dr. Miquel Salmeron. From 1994–1996, he was a postdoc at MIT developing MEMS under Martin Schmidt (EECS) and Mark Wrighton (Chemistry). In 1997, he joined Mehmet Toner’s lab as a postdoc at Harvard-MGH to apply soft lithography to tissue engineering. He has been at Seattle’s UW BioE since June 2000, where he is now a full Professor, accumulating over 14,850 citations (h-index 55; career average 150 citations/paper). In 25 years, he has graduated 20 postdocs (20% of whom have reached faculty rank) and 33 graduate students (11 Ph.D. students, 27% of whom faculty rank, and 22 M.S. students). In 2001 he received an NSF Career Award, and in 2014 he was elected to the AIMBE College of Fellows (Class of 2015). He serves on the Advisory Board of Lab on a Chip since 2010 and on the Editorial Board of Micromachines since 2019. In 2022 he was elected a member of the Institute for Catalan Studies, one of the highest honors bestowed on Catalan scientists, and his book “Hidden in Plain Sight” won winner of the 2022 Outstanding Academic Title Award by Choice. He is the author of 6 books (sole author), including Introduction to BioMEMS (2012, Taylor&Francis), a textbook adopted by >103 departments in 18 countries, Hidden in Plain Sight: The History, Science, and Engineering of Microfluidic Technology (MIT Press, 2022), and the recent How the World Flows: Microfluidics from Raindrops to Covid Tests (Oxford University Press, 2025). Since 2007, the lab runs a celebrated outreach art program called BAIT (Bringing Art Into Technology), which has produced eight exhibits, a popular resource gallery of >2,000 free images related to microfluidics and microfabrication, and a YouTube channel that plays microfluidic videos with music which accumulate >177,000 visits since 2009.

Transversal skills. Begonya Nafria Escalera
2026-01-23 10:00 - 11:00
Involucrar als pacients, no és una moda passatjera
Begonya Nafria Escalera, Patient Engagement in Research Head of Department at Sant Joan de Déu Chidren’s Hospital (Spain)
La recerca i la innovació que respongui a les necessitats dels pacients, requereix involucrar-los en les fases inicials i durant la vida d’un projecte. En aquesta sessió parlarem sobre la nova ciència que és el “patient involvement”, aportant també projectes inscpiradors que demostrin els beneficis d’involucrar als pacients en l’àmbit de les ciències de la salut.

Ibec Seminar. Silke Henkes
2026-01-26 16:00 - 17:00
Mechanochemical feedback in model epithelial tissues
Silke Henkes, Leiden Institute of Physics, Leiden University
In the development of animals, tissues self-organise starting from a single cell into layers, shapes and patterns. This active mechanical process operates beyond the theoretical framework of reaction-diffusion equations such as Turing patterns. At the same time, combining active driving with careful mechanical design of a system is distinct route to pattern formation and artificial functionality.
Here I will show how two types of active driving interacts with mechanics: First, for polar active materials where cells crawl on a substrate, even with only uncorrelated activity, elasticity leads to
the emergence of mesoscopic space-time correlations. I will show how this explains the ‘fingering instability’ at the edge of MDCK epithelial cell sheets as long lived active correlations. If we add alignment to generate a flocking state, and cell division and death, we model corneal epithelial cells well. We are then able to simulate the whole curved corneal epithelium, and show that an inflow of cells from the limbus generates a stable spiral flow pattern with a +1 topological defect at the centre.
Second, mechanochemical stress feedback in cell-cell junctions arises from the catch bond dynamics of the actomyosin cortex. It allows a junction to generate a contractile force that can overcome external pulling and thus allow for an active rearrangement or T1. In vertex and continuum models, for strong enough feedback this gives rise to convergence-extension flows where the flow is opposite the direction of mechanical polarisation, effectively generating a negative viscosity state.

Ibec Seminar. Francisco Lorenzo-Martín
2026-01-30 09:30 - 10:30
Next-generation organoid models for colorectal cancer research
Francisco Lorenzo-Martín, University of Salamanca

Three-dimensional organoid culture technologies have revolutionized cancer research by allowing for more realistic and scalable reproductions of tumor structures. This has enabled better modelling of low-complexity cancer cell behaviors that occur over short periods of time. However, the currently available organoid systems lack the requisite multi-cellular diversity, tissue-level organization, biological durability, and experimental flexibility that are needed to capture complex oncological processes. Consequently, the study of many multifactorial cancer mechanisms is still not possible in vitro and instead requires the extensive use of animal models, which provide limited spatiotemporal resolution of cellular dynamics and come at a substantial ethical cost. To overcome these limitations, we have implemented tissue engineering and microfabrication technologies to develop topobiologically complex ex vivo cancer avatars. Focusing on colorectal cancer, we have generated miniature tissues consisting of long-lived gut-shaped colon epithelia (‘mini-colons’) that are able to undergo tumorigenesis in vitro and stably integrate cancer cells with their native tumor microenvironment. This system provides an unprecedented repertoire of experimental possibilities, which we illustrate through diverse applications. Altogether, these next-generation organoid models push the boundaries of ex vivo cancer research.

Ibec Seminar. Prof. Kara Spiller
2026-04-17 11:00
Engineering Macrophages with Biomaterials for Regenerative Medicine
Kara L. Spiller, PhD, URBN Professor of Biomedical Innovation, School of Biomedical Engineering, Science, and Health Systems, Drexel University, Philadelphia, PA, USA
The inflammatory response, orchestrated primarily by macrophages, plays a major role in the body’s response to injury, disease, or implantation of a biomaterial. When macrophages function normally, they are a powerful force that promote tissue repair and regeneration, but when their behavior goes awry, healing is impaired. Our goal is to understand the mechanisms by which the inflammatory response orchestrates successful tissue regeneration and to develop novel biomaterial strategies that apply these principles to situations in which tissue regeneration is impaired. In particular, we study macrophages in inflammation, phagocytosis, cellular recruitment, vascularization, cell proliferation, tissue deposition, or remodeling, and we design biomaterials that leverage these findings for improved biomaterials design, enhanced macrophage cell therapy, and personalized medicine. Current projects in the lab are directed towards treatment of pulmonary fibrosis and chronic wound healing through biomaterial-enabled macrophage cell therapies.
Dr. Kara Spiller is the URBN Endowed Professor of Biomedical Innovation in Drexel University’s School of Biomedical Engineering, Science, and Health Systems. Her research focuses on the role of immune cells in tissue repair and regeneration and the design of immunomodulatory biomaterials. Her research is funded by the NIH, the NSF, private foundations, and industry. Her awards include a Fulbright fellowship, the NSF CAREER award, and the United States Dept. of State ASPIRE prize. She has published more than 85 publications in biomaterials and immune engineering, and has 18 issued or pending patents. She is the founding director of the Immune Modulation & Engineering Symposium series in Philadelphia, which is the only annually recurring meeting dedicated to convergent research in translational immunology and engineering. She serves on the board of numerous journals and is a frequent NIH study section member.
