BEGIN:VCALENDAR VERSION:2.0 PRODID:-//Institute for Bioengineering of Catalonia - ECPv6.15.20//NONSGML v1.0//EN CALSCALE:GREGORIAN METHOD:PUBLISH X-WR-CALNAME:Institute for Bioengineering of Catalonia X-ORIGINAL-URL:https://ibecbarcelona.eu X-WR-CALDESC:Events for Institute for Bioengineering of Catalonia REFRESH-INTERVAL;VALUE=DURATION:PT1H X-Robots-Tag:noindex X-PUBLISHED-TTL:PT1H BEGIN:VTIMEZONE TZID:Europe/Madrid BEGIN:DAYLIGHT TZOFFSETFROM:+0100 TZOFFSETTO:+0200 TZNAME:CEST DTSTART:20140330T010000 END:DAYLIGHT BEGIN:STANDARD TZOFFSETFROM:+0200 TZOFFSETTO:+0100 TZNAME:CET DTSTART:20141026T010000 END:STANDARD BEGIN:DAYLIGHT TZOFFSETFROM:+0100 TZOFFSETTO:+0200 TZNAME:CEST DTSTART:20150329T010000 END:DAYLIGHT BEGIN:STANDARD TZOFFSETFROM:+0200 TZOFFSETTO:+0100 TZNAME:CET DTSTART:20151025T010000 END:STANDARD BEGIN:DAYLIGHT TZOFFSETFROM:+0100 TZOFFSETTO:+0200 TZNAME:CEST DTSTART:20160327T010000 END:DAYLIGHT BEGIN:STANDARD TZOFFSETFROM:+0200 TZOFFSETTO:+0100 TZNAME:CET DTSTART:20161030T010000 END:STANDARD END:VTIMEZONE BEGIN:VTIMEZONE TZID:UTC BEGIN:STANDARD TZOFFSETFROM:+0000 TZOFFSETTO:+0000 TZNAME:UTC DTSTART:20140101T000000 END:STANDARD END:VTIMEZONE BEGIN:VEVENT DTSTART;TZID=Europe/Madrid:20150320T100000 DTEND;TZID=Europe/Madrid:20150320T110000 DTSTAMP:20260419T072517 CREATED:20150119T103657Z LAST-MODIFIED:20150317T104438Z UID:11715-1426845600-1426849200@ibecbarcelona.eu SUMMARY:IBEC Seminar: Romain Quidant DESCRIPTION:Biomedical applications of nanoplasmonics\n \nRomain Quidant\, ICREA professor at Institut de Ciències Fotòniques (ICFO)\nGold nanostructures\, supporting localized surface plasmon resonances\, can be designed to act upon illumination as efficient point-like sources of either light or heat\, opening plenty of new science and applications in biology and medicine. In this talk we discuss how both their optical and photothermal properties can be exploited to develop alternative\, minimally invasive strategies for the detection and therapy of cancer. \nThe first part of this presentation focuses on the use of the intense and confined optical fields bound to gold nanostructures for biosensing and optical trapping. In the frame of sensing\, we show that gold nanostructures lithographically prepared on glass can be engineered as compact and highly sensitive sensors to detect low concentrations of cancer markers in serum. As for optical trapping\, we demonstrate that plasmonic fields enable creating on-a-chip nano-optical tweezers able to trap a large number of micro- and nano- specimens with a single laser beam of low laser intensity. \nIn the second part we talk about the use of gold nanoparticles as remotely controlled point-like sources of heat for photothermal cancer therapy. We first discuss\, both theoretically and experimentally\, the physics of heat generation at the nanoscale along with the effect of the particle shape and the illumination. We then present the use of conjugated gold nanoparticles for specific cancer cell hyperthermia. URL:https://ibecbarcelona.eu/event/ibec-seminar-romain-quidant/ CATEGORIES:IBEC Seminar END:VEVENT BEGIN:VEVENT DTSTART;TZID=Europe/Madrid:20150320T100000 DTEND;TZID=Europe/Madrid:20150320T110000 DTSTAMP:20260419T072517 CREATED:20150119T103657Z LAST-MODIFIED:20150119T103657Z UID:95834-1426845600-1426849200@ibecbarcelona.eu SUMMARY:IBEC Seminar: Romain Quidant DESCRIPTION:Biomedical applications of nanoplasmonics\n \nRomain Quidant\, ICREA professor at Institut de Ciències Fotòniques (ICFO)\nGold nanostructures\, supporting localized surface plasmon resonances\, can be designed to act upon illumination as efficient point-like sources of either light or heat\, opening plenty of new science and applications in biology and medicine. In this talk we discuss how both their optical and photothermal properties can be exploited to develop alternative\, minimally invasive strategies for the detection and therapy of cancer. \nThe first part of this presentation focuses on the use of the intense and confined optical fields bound to gold nanostructures for biosensing and optical trapping. In the frame of sensing\, we show that gold nanostructures lithographically prepared on glass can be engineered as compact and highly sensitive sensors to detect low concentrations of cancer markers in serum. As for optical trapping\, we demonstrate that plasmonic fields enable creating on-a-chip nano-optical tweezers able to trap a large number of micro- and nano- specimens with a single laser beam of low laser intensity. \nIn the second part we talk about the use of gold nanoparticles as remotely controlled point-like sources of heat for photothermal cancer therapy. We first discuss\, both theoretically and experimentally\, the physics of heat generation at the nanoscale along with the effect of the particle shape and the illumination. We then present the use of conjugated gold nanoparticles for specific cancer cell hyperthermia. URL:https://ibecbarcelona.eu/event/ibec-seminar-romain-quidant-2/ CATEGORIES:IBEC Seminar END:VEVENT BEGIN:VEVENT DTSTART;TZID=Europe/Madrid:20150410T100000 DTEND;TZID=Europe/Madrid:20150410T110000 DTSTAMP:20260419T072517 CREATED:20150119T102356Z LAST-MODIFIED:20150119T102356Z UID:95822-1428660000-1428663600@ibecbarcelona.eu SUMMARY:IBEC Seminar: Núria Montserrat DESCRIPTION:Dreaming of organ regeneration: new hopes for regenerative medicine\n \nNúria Montserrat\, Pluripotent stem cells and activation of endogenous tissue programs for organ regeneration group\, IBEC\nDr. Montserrat became interested in organ regeneration and stem cells during her master in biological research and PhD training. She completed her PhD with Dr. Joaquim Gutiérrez and Isabel Navarro at the Faculty of Biology\, University of Barcelona in 2006. During her master and PhD studies\, she set-up in vitro platforms for the study of skeletal muscle stem cells during homeostasis and under different metabolic conditions. During her PhD studies she did several stages at other European laboratories: Manfred Reinecke’s laboratory at the University of Zurich and at Jean-Charles Gabillard’s laboratory in INRA SCRIBE\, France. In 2006\,Dr. Montserrat got her European PhD degree at the University of Barcelona. \nIn 2006 she got a Postdoctoral fellowship from the Fundaçao para a Ciência e Tecnología (Portugal) and in 2007 she was hired as a post-doctoral researcher at at the Hospital of Santa Creu i Sant Pau in Barcelona. Early in 2008 she joined the Center of Regenerative Medicine of Barcelona (CMRB) with the objective to study the molecular mechanisms driving tissue regeneration\, somatic reprogramming and disease modeling. \nThe research line of Dr.Montserrat has been awarded with an ERC Starting Grant within the call of 2014. Recently\, Dr. Montserrat has joined IBEC as Junior Group leader. Her aim is to develop strategies for tissue and organ engineering making use of pluripotent stem cells from patients and the reactivation of tissue endogenous programs. URL:https://ibecbarcelona.eu/event/ibec-seminar-nuria-montserrat-2/ CATEGORIES:IBEC Seminar END:VEVENT BEGIN:VEVENT DTSTART;TZID=Europe/Madrid:20150410T100000 DTEND;TZID=Europe/Madrid:20150410T110000 DTSTAMP:20260419T072517 CREATED:20150119T102356Z LAST-MODIFIED:20150309T095504Z UID:11713-1428660000-1428663600@ibecbarcelona.eu SUMMARY:IBEC Seminar: Núria Montserrat DESCRIPTION:Dreaming of organ regeneration: new hopes for regenerative medicine\n \nNúria Montserrat\, Pluripotent stem cells and activation of endogenous tissue programs for organ regeneration group\, IBEC\nDr. Montserrat became interested in organ regeneration and stem cells during her master in biological research and PhD training. She completed her PhD with Dr. Joaquim Gutiérrez and Isabel Navarro at the Faculty of Biology\, University of Barcelona in 2006. During her master and PhD studies\, she set-up in vitro platforms for the study of skeletal muscle stem cells during homeostasis and under different metabolic conditions. During her PhD studies she did several stages at other European laboratories: Manfred Reinecke’s laboratory at the University of Zurich and at Jean-Charles Gabillard’s laboratory in INRA SCRIBE\, France. In 2006\,Dr. Montserrat got her European PhD degree at the University of Barcelona. \nIn 2006 she got a Postdoctoral fellowship from the Fundaçao para a Ciência e Tecnología (Portugal) and in 2007 she was hired as a post-doctoral researcher at at the Hospital of Santa Creu i Sant Pau in Barcelona. Early in 2008 she joined the Center of Regenerative Medicine of Barcelona (CMRB) with the objective to study the molecular mechanisms driving tissue regeneration\, somatic reprogramming and disease modeling. \nThe research line of Dr.Montserrat has been awarded with an ERC Starting Grant within the call of 2014. Recently\, Dr. Montserrat has joined IBEC as Junior Group leader. Her aim is to develop strategies for tissue and organ engineering making use of pluripotent stem cells from patients and the reactivation of tissue endogenous programs. URL:https://ibecbarcelona.eu/event/ibec-seminar-nuria-montserrat/ CATEGORIES:IBEC Seminar END:VEVENT BEGIN:VEVENT DTSTART;TZID=Europe/Madrid:20150508T100000 DTEND;TZID=Europe/Madrid:20150508T110000 DTSTAMP:20260419T072517 CREATED:20150311T074930Z LAST-MODIFIED:20150311T074930Z UID:95839-1431079200-1431082800@ibecbarcelona.eu SUMMARY:IBEC Seminar: J. Miguel Rubi DESCRIPTION:Somatic exocytosis of serotonin mediated by molecular motors\n \nJ. Miguel Rubi\, Departament de Fisica Fonamental\, Universitat de Barcelona\nWe quantified somatic exocytosis of serotonin in Retzius neurons and explored the possible contribution of molecular motors and the cytoskeleton on the mobilization of vesicles induced by stimulation with trains of electrical impulses. Secretion was quantified from the increase of fluorescence of FM1-43 in response to sequences of 20 mV trains of 10 impulses at 2 min intervals\, produced by intracellular current injection. Somatic secretion was also evoked by a pulse of 10 mM caffeine applied to the bathing solution. Stimulation of neurons produced a gradual increase in FM1-43 fluorescence for over the next five minutes. The kinetics and latencies of these increases varied from one neuron to another but usually maintaining a sigmoidal shape in one or two steps. Neurons stimulation in the presence of colchicine to uncouple microtubules\, failed to evoke fluorescence increases\, thus suggesting that vesicle mobilization depended on tubulin-based motor. The kinetics of the fluorescence increases of individual neurons was accounted for by using a model based on a diffusion equation in the presence of external forces\, consistent with the contribution of molecular motors to the mobilization of the vesicle clusters towards the membrane in response to electrical activity. Our data show that somatic serotonin secretion in Retzius neurons depends on a motor-based cytoskeletal mobilization of vesicles induced by electrical activity. URL:https://ibecbarcelona.eu/event/15400-2/ CATEGORIES:IBEC Seminar END:VEVENT BEGIN:VEVENT DTSTART;TZID=Europe/Madrid:20150508T100000 DTEND;TZID=Europe/Madrid:20150508T110000 DTSTAMP:20260419T072517 CREATED:20150311T074930Z LAST-MODIFIED:20150505T144616Z UID:15400-1431079200-1431082800@ibecbarcelona.eu SUMMARY:IBEC Seminar: J. Miguel Rubi DESCRIPTION:Somatic exocytosis of serotonin mediated by molecular motors\n \nJ. Miguel Rubi\, Departament de Fisica Fonamental\, Universitat de Barcelona\nWe quantified somatic exocytosis of serotonin in Retzius neurons and explored the possible contribution of molecular motors and the cytoskeleton on the mobilization of vesicles induced by stimulation with trains of electrical impulses. Secretion was quantified from the increase of fluorescence of FM1-43 in response to sequences of 20 mV trains of 10 impulses at 2 min intervals\, produced by intracellular current injection. Somatic secretion was also evoked by a pulse of 10 mM caffeine applied to the bathing solution. Stimulation of neurons produced a gradual increase in FM1-43 fluorescence for over the next five minutes. The kinetics and latencies of these increases varied from one neuron to another but usually maintaining a sigmoidal shape in one or two steps. Neurons stimulation in the presence of colchicine to uncouple microtubules\, failed to evoke fluorescence increases\, thus suggesting that vesicle mobilization depended on tubulin-based motor. The kinetics of the fluorescence increases of individual neurons was accounted for by using a model based on a diffusion equation in the presence of external forces\, consistent with the contribution of molecular motors to the mobilization of the vesicle clusters towards the membrane in response to electrical activity. Our data show that somatic serotonin secretion in Retzius neurons depends on a motor-based cytoskeletal mobilization of vesicles induced by electrical activity. URL:https://ibecbarcelona.eu/event/15400/ CATEGORIES:IBEC Seminar END:VEVENT BEGIN:VEVENT DTSTART;TZID=Europe/Madrid:20150522T100000 DTEND;TZID=Europe/Madrid:20150522T110000 DTSTAMP:20260419T072517 CREATED:20150408T132341Z LAST-MODIFIED:20150408T132405Z UID:15816-1432288800-1432292400@ibecbarcelona.eu SUMMARY:IBEC Seminar: Thomas Graf DESCRIPTION:Mechanisms of transcription factor induced transdifferentiation and reprogramming to pluripotency\n \nThomas Graf\, Gene Regulation\, Stem Cells and Differentiation Programme\, CRG / Pompeu Fabra University\nWork by many laboratories has shown that even fully differentiated cells are remarkably plastic and can be reprogrammed into alternative fates. This discovery has revolutionized our understanding of how cell decide what to become and has major implications for the modeling and therapy of diseases that affect the production of differentiated cells. \nOur earlier work has shown that the myeloid transcription factor C/EBPa induces B cells to transdifferentiate into macrophages at high efficiencies. We have now found that the process forces the intersection of two enhancer pathways that become activated during normal hematopoietic differentiation. \nRecently we reported that the transient expression of C/EBPa in B cells\, followed by expression of the pluripotency factors Oct4\, Sox2\, Klf4 and c-Myc (OSKM)\, poises the cells for very rapid and highly efficient reprogramming into induced pluripotent stem cells. Our findings have removed a major obstacle in studying cell reprogramming and permitted us to investigate how C/EBPa leads to the almost immediate accessibility of pluripotency genes to binding by Oct4. Our new data provide unprecedented insights into the earliest events leading to activation of the pluripotency gene regulatory network\, resulting in somatic cell reprogramming. URL:https://ibecbarcelona.eu/event/ibec-seminar-thomas-graf/ CATEGORIES:IBEC Seminar END:VEVENT BEGIN:VEVENT DTSTART;TZID=Europe/Madrid:20150522T100000 DTEND;TZID=Europe/Madrid:20150522T110000 DTSTAMP:20260419T072517 CREATED:20150408T132341Z LAST-MODIFIED:20150408T132341Z UID:95842-1432288800-1432292400@ibecbarcelona.eu SUMMARY:IBEC Seminar: Thomas Graf DESCRIPTION:Mechanisms of transcription factor induced transdifferentiation and reprogramming to pluripotency\n \nThomas Graf\, Gene Regulation\, Stem Cells and Differentiation Programme\, CRG / Pompeu Fabra University\nWork by many laboratories has shown that even fully differentiated cells are remarkably plastic and can be reprogrammed into alternative fates. This discovery has revolutionized our understanding of how cell decide what to become and has major implications for the modeling and therapy of diseases that affect the production of differentiated cells. \nOur earlier work has shown that the myeloid transcription factor C/EBPa induces B cells to transdifferentiate into macrophages at high efficiencies. We have now found that the process forces the intersection of two enhancer pathways that become activated during normal hematopoietic differentiation. \nRecently we reported that the transient expression of C/EBPa in B cells\, followed by expression of the pluripotency factors Oct4\, Sox2\, Klf4 and c-Myc (OSKM)\, poises the cells for very rapid and highly efficient reprogramming into induced pluripotent stem cells. Our findings have removed a major obstacle in studying cell reprogramming and permitted us to investigate how C/EBPa leads to the almost immediate accessibility of pluripotency genes to binding by Oct4. Our new data provide unprecedented insights into the earliest events leading to activation of the pluripotency gene regulatory network\, resulting in somatic cell reprogramming. URL:https://ibecbarcelona.eu/event/ibec-seminar-thomas-graf-2/ CATEGORIES:IBEC Seminar END:VEVENT BEGIN:VEVENT DTSTART;TZID=Europe/Madrid:20150612T100000 DTEND;TZID=Europe/Madrid:20150612T110000 DTSTAMP:20260419T072517 CREATED:20150311T075156Z LAST-MODIFIED:20150608T143446Z UID:15401-1434103200-1434106800@ibecbarcelona.eu SUMMARY:IBEC Seminar: Alexandra P. Marques DESCRIPTION:Driving skin wound healing: stem cells and extracellular matrix role\n \nAlexandra P. Marques\, 3B’s Research Group\, University of Minho\, Portugal\nWound care products have evolved into skin tissue engineered substitutes\, which despite the longest history of application and the highest record of marketing\, proved to represent replacement strategies instead of promoting tissue regeneration. \nCurrent research indicates that the interactions between resident progenitor cells and their niche dictate the triggering of skin regeneration pathway. In alignment\, mesenchymal stem cells (MSCs)-based therapies\, have been proposed to enhance cutaneous wound healing.  The rationale lies on transplanted cells ability to interact/respond to the wound microenvironment\, which is advantageous when compared to the exogenous administration of healing factors. However\, the involved mechanisms are still elusive and poor outcomes were achieved in terms of attainment of functional skin tissue due to low cell survival rates\, and poor engraftment or cell fusion upon transplantation.  Extracellular matrix (ECM)-mimicking is currently seen as the “Holy Grail” of Tissue Engineering in the sense that by recreating natural tissues microenvironments researchers will be able to increase the residence time and consequently the action of the transplanted cells and thus uncover “therapeutic niches”. \nUnder this context we have been exploring two perspectives; one takes advantage of the tunable ECM-like properties along with the 3D support that hydrogels can provide\, and the second benefits from an intact native ECM offered by cell sheet engineering technology. Hydrogels features such as high hygroscopic nature\, tunable elasticity and facilitated mass transportation\, render such materials attractive for the development of skin ECM-analogues. Although succeeding in improved cell engraftment\, hydrogels fail to provide biological instructive cues as well as cell adhesion sites\, only overcome by cell adhesive peptides bonding to polymers backbone. We developed a new method of processing gellan gum-based materials\, having as start material gellan gum-based hydrogels\, to obtain structures that exhibit features of both sponges and hydrogels depicting intrinsic cell-adhesive properties. By creating constructs comprising adipose tissue cells and artificial and natural ECM we were able to demonstrated that skin healing is dependent on tissue engineered constructs self cell-cell and cell-ECM interactions\, as well as on constructs cell-cell interactions and paracrine signaling with resident cells. In particular\, our results suggest that cell-ECM and cell-cell interactions have a dramatic effect over re-epithelialisation. In opposition\, neo-vascularisation did not seem to be dependent on the nature of the cell-ECM interactions\, but was significantly improved with the incorporation of microvascular endothelial cells. \nUltimately the generation of knowledge on how to direct skin regeneration lead the creation of “off-the-shelf” 3D stem cell-based engineered products inspired by the role of wound healing microenvironments. URL:https://ibecbarcelona.eu/event/ibec-seminar-alexandra-p-marques/ CATEGORIES:IBEC Seminar END:VEVENT BEGIN:VEVENT DTSTART;TZID=Europe/Madrid:20150612T100000 DTEND;TZID=Europe/Madrid:20150612T110000 DTSTAMP:20260419T072517 CREATED:20150311T075156Z LAST-MODIFIED:20150311T075156Z UID:95840-1434103200-1434106800@ibecbarcelona.eu SUMMARY:IBEC Seminar: Alexandra P. Marques DESCRIPTION:Driving skin wound healing: stem cells and extracellular matrix role\n \nAlexandra P. Marques\, 3B’s Research Group\, University of Minho\, Portugal\nWound care products have evolved into skin tissue engineered substitutes\, which despite the longest history of application and the highest record of marketing\, proved to represent replacement strategies instead of promoting tissue regeneration. \nCurrent research indicates that the interactions between resident progenitor cells and their niche dictate the triggering of skin regeneration pathway. In alignment\, mesenchymal stem cells (MSCs)-based therapies\, have been proposed to enhance cutaneous wound healing.  The rationale lies on transplanted cells ability to interact/respond to the wound microenvironment\, which is advantageous when compared to the exogenous administration of healing factors. However\, the involved mechanisms are still elusive and poor outcomes were achieved in terms of attainment of functional skin tissue due to low cell survival rates\, and poor engraftment or cell fusion upon transplantation.  Extracellular matrix (ECM)-mimicking is currently seen as the “Holy Grail” of Tissue Engineering in the sense that by recreating natural tissues microenvironments researchers will be able to increase the residence time and consequently the action of the transplanted cells and thus uncover “therapeutic niches”. \nUnder this context we have been exploring two perspectives; one takes advantage of the tunable ECM-like properties along with the 3D support that hydrogels can provide\, and the second benefits from an intact native ECM offered by cell sheet engineering technology. Hydrogels features such as high hygroscopic nature\, tunable elasticity and facilitated mass transportation\, render such materials attractive for the development of skin ECM-analogues. Although succeeding in improved cell engraftment\, hydrogels fail to provide biological instructive cues as well as cell adhesion sites\, only overcome by cell adhesive peptides bonding to polymers backbone. We developed a new method of processing gellan gum-based materials\, having as start material gellan gum-based hydrogels\, to obtain structures that exhibit features of both sponges and hydrogels depicting intrinsic cell-adhesive properties. By creating constructs comprising adipose tissue cells and artificial and natural ECM we were able to demonstrated that skin healing is dependent on tissue engineered constructs self cell-cell and cell-ECM interactions\, as well as on constructs cell-cell interactions and paracrine signaling with resident cells. In particular\, our results suggest that cell-ECM and cell-cell interactions have a dramatic effect over re-epithelialisation. In opposition\, neo-vascularisation did not seem to be dependent on the nature of the cell-ECM interactions\, but was significantly improved with the incorporation of microvascular endothelial cells. \nUltimately the generation of knowledge on how to direct skin regeneration lead the creation of “off-the-shelf” 3D stem cell-based engineered products inspired by the role of wound healing microenvironments. URL:https://ibecbarcelona.eu/event/ibec-seminar-alexandra-p-marques-2/ CATEGORIES:IBEC Seminar END:VEVENT BEGIN:VEVENT DTSTART;TZID=Europe/Madrid:20150616T160000 DTEND;TZID=Europe/Madrid:20150616T170000 DTSTAMP:20260419T072517 CREATED:20150604T150254Z LAST-MODIFIED:20150604T150254Z UID:95856-1434470400-1434474000@ibecbarcelona.eu SUMMARY:IBEC Seminar: Javier G. Fernández DESCRIPTION:Bioinspired materials\n \nJavier G. Fernández\, Assistant Professor and Founder Academic Member · Singapore University of Technology and Design\nNature is abundant with materials that possess extraordinary mechanical characteristics. New techniques in biochemistry and advances in microelectronic engineering are boosting our knowledge of biological materials\, and providing tools to fabricate at the scale at which these materials are made in nature. This investigation of the structural organization of these materials leads to understanding of the principles of natural materials design that are beginning to be harnessed to fabricate biologically-inspired composites for materials engineering with tunable properties that mimic living materials\, which might provide useful for environmental challenges\, as well as medical applications. URL:https://ibecbarcelona.eu/event/ibec-seminar-javier-g-fernandez-2/ CATEGORIES:IBEC Seminar END:VEVENT BEGIN:VEVENT DTSTART;TZID=Europe/Madrid:20150616T160000 DTEND;TZID=Europe/Madrid:20150616T170000 DTSTAMP:20260419T072517 CREATED:20150604T150254Z LAST-MODIFIED:20150604T150254Z UID:16893-1434470400-1434474000@ibecbarcelona.eu SUMMARY:IBEC Seminar: Javier G. Fernández DESCRIPTION:Bioinspired materials\n \nJavier G. Fernández\, Assistant Professor and Founder Academic Member · Singapore University of Technology and Design\nNature is abundant with materials that possess extraordinary mechanical characteristics. New techniques in biochemistry and advances in microelectronic engineering are boosting our knowledge of biological materials\, and providing tools to fabricate at the scale at which these materials are made in nature. This investigation of the structural organization of these materials leads to understanding of the principles of natural materials design that are beginning to be harnessed to fabricate biologically-inspired composites for materials engineering with tunable properties that mimic living materials\, which might provide useful for environmental challenges\, as well as medical applications. URL:https://ibecbarcelona.eu/event/ibec-seminar-javier-g-fernandez/ CATEGORIES:IBEC Seminar END:VEVENT BEGIN:VEVENT DTSTART;TZID=Europe/Madrid:20150619T100000 DTEND;TZID=Europe/Madrid:20150619T110000 DTSTAMP:20260419T072517 CREATED:20150410T120745Z LAST-MODIFIED:20150410T120745Z UID:95844-1434708000-1434711600@ibecbarcelona.eu SUMMARY:IBEC Seminar: Matteo Palma DESCRIPTION:Bio-inspired self-assembly for single-molecule investigations \n \nMatteo Palma\, Queen Mary University of London\nOne of the ultimate goals in nanotechnology is the ability to produce efficient devices based on individual molecules and nanostructures. Despite the many potential benefits envisioned for single-molecule technology (in electronics and biotechnology) the strategies employed to date suffer from various limitations. Principal among these limitations is the poor control over the molecular assembly of nanostructures and individual molecules with respect to one another\, as well as their position on devices with respect to other material components. \nI will first discuss techniques based on the combined use of lithographic nanopatterning and bio-molecular self-assembly to control the immobilization of biomolecules in arrayed nanodomains. I will show how this allows us to produce highly ordered\, self-assembled arrangements of nano-objects\, ranging from proteins to DNA nanostructures\, and bio-inorganic assemblies\, for a variety of (nanoscale) investigations. \nI will show how by specific design of the biomolecular nanoarrays\, it is possible to simultaneously monitor hundreds of protein/DNA binding events at the single-molecule level. Moreover I will discuss the use of our nanopatterned biomimetic surfaces to probe the importance of transmembrane proteins (integrins) clustering and geometric arrangement of binding sites\, in the formation of cell focal adhesions \nI will then highlight the broader utility of such nanopatterned surfaces for the self-organization (on surfaces) of bio-inorganic assemblies as well as DNA nanostructures and carbon nanotubes. In particular\, I will discuss how the combination of high resolution patterning with end-functional chemistry enables the assembly of 1D functional nanostructures in an orderly fashion. \nFinally\, building on our novel bottom-up assembly strategy for the formation of (chemically and geometrically) versatile carbon nanotube (CNTs) junctions\, I will present a universal approach for the generation of multifunctional nanomaterials that employ molecular building blocks assembled between DNA wrapped CNT electrodes. We will demonstrate single-molecule control in the formation of nanohybrids via the in-solution assembly of classes of molecular materials (organic\, and inorganic which display promising attributes) to DNA wrapped CNTs. We believe this may be a viable avenue towards the integration of these materials in complex and functional nano-architectures. \nOur findings are of general interest for the controlled assembly of a broad range of functional molecules and nanostructures\, towards the fabrication of solution-processable nanoscale devices. Moreover\, we believe that the knowledge developed makes a significant contribution towards the facile fabrication of nanohybrid materials for single-molecule investigations. Future technologies will require devices of this type in a variety of key areas\, including biodiagnostics\, ultra-high speed computation\, bioelectronics\, and for renewable energy applications. URL:https://ibecbarcelona.eu/event/ibec-seminar-matteo-palma-2/ CATEGORIES:IBEC Seminar END:VEVENT BEGIN:VEVENT DTSTART;TZID=Europe/Madrid:20150619T100000 DTEND;TZID=Europe/Madrid:20150619T110000 DTSTAMP:20260419T072517 CREATED:20150410T120745Z LAST-MODIFIED:20150616T113709Z UID:15930-1434708000-1434711600@ibecbarcelona.eu SUMMARY:IBEC Seminar: Matteo Palma DESCRIPTION:Bio-inspired self-assembly for single-molecule investigations \n \nMatteo Palma\, Queen Mary University of London\nOne of the ultimate goals in nanotechnology is the ability to produce efficient devices based on individual molecules and nanostructures. Despite the many potential benefits envisioned for single-molecule technology (in electronics and biotechnology) the strategies employed to date suffer from various limitations. Principal among these limitations is the poor control over the molecular assembly of nanostructures and individual molecules with respect to one another\, as well as their position on devices with respect to other material components. \nI will first discuss techniques based on the combined use of lithographic nanopatterning and bio-molecular self-assembly to control the immobilization of biomolecules in arrayed nanodomains. I will show how this allows us to produce highly ordered\, self-assembled arrangements of nano-objects\, ranging from proteins to DNA nanostructures\, and bio-inorganic assemblies\, for a variety of (nanoscale) investigations. \nI will show how by specific design of the biomolecular nanoarrays\, it is possible to simultaneously monitor hundreds of protein/DNA binding events at the single-molecule level. Moreover I will discuss the use of our nanopatterned biomimetic surfaces to probe the importance of transmembrane proteins (integrins) clustering and geometric arrangement of binding sites\, in the formation of cell focal adhesions \nI will then highlight the broader utility of such nanopatterned surfaces for the self-organization (on surfaces) of bio-inorganic assemblies as well as DNA nanostructures and carbon nanotubes. In particular\, I will discuss how the combination of high resolution patterning with end-functional chemistry enables the assembly of 1D functional nanostructures in an orderly fashion. \nFinally\, building on our novel bottom-up assembly strategy for the formation of (chemically and geometrically) versatile carbon nanotube (CNTs) junctions\, I will present a universal approach for the generation of multifunctional nanomaterials that employ molecular building blocks assembled between DNA wrapped CNT electrodes. We will demonstrate single-molecule control in the formation of nanohybrids via the in-solution assembly of classes of molecular materials (organic\, and inorganic which display promising attributes) to DNA wrapped CNTs. We believe this may be a viable avenue towards the integration of these materials in complex and functional nano-architectures. \nOur findings are of general interest for the controlled assembly of a broad range of functional molecules and nanostructures\, towards the fabrication of solution-processable nanoscale devices. Moreover\, we believe that the knowledge developed makes a significant contribution towards the facile fabrication of nanohybrid materials for single-molecule investigations. Future technologies will require devices of this type in a variety of key areas\, including biodiagnostics\, ultra-high speed computation\, bioelectronics\, and for renewable energy applications. URL:https://ibecbarcelona.eu/event/ibec-seminar-matteo-palma/ CATEGORIES:IBEC Seminar END:VEVENT BEGIN:VEVENT DTSTART;TZID=Europe/Madrid:20150706T150000 DTEND;TZID=Europe/Madrid:20150706T160000 DTSTAMP:20260419T072517 CREATED:20150701T122222Z LAST-MODIFIED:20150701T122253Z UID:17897-1436194800-1436198400@ibecbarcelona.eu SUMMARY:IBEC Seminar: Prof. Saman K. Halgamuge DESCRIPTION:Big Data Analytics in Metagenomics\nSaman K. Halgamuge\, Melbourne School of Engineering\, University of Melbourne\, Australia\nIn collaboration with researchers in Academia Sinica and Metabolomics Australia/Department of Botany at Melbourne\, we have been working in two areas of Bioinformatics: Metabolomics focusing on microbes and Metagenomics focusing on plants. Profiling large sets of data resulted from technological advances in whole genome sequencing and MALDI Imaging type technologies that can reveal vital information about the environment and plants\, which is our major or primary source of food on Earth. Recently we have demonstrated considerable success in using unsupervised clustering techniques to analyse genetic and metabolomic data. This includes analysis of viral quasi species\, metabolomics and microbial metagenomes. \nSome microbes in the environment appear to look very similar and found “living together” in communities in non-separable ways\, making them harder to culture in a lab. To make matters worst\, considering our belief\, if it is correct at all\, that we know only about up to 2% of the microbes around us. When we know only so little about the data labels\, in this case\, about the identity of the species. It is even more challenging to recognise patterns associated with the genomes of the quasispecies (a set of genetically related but non-identical viral mutant types\, which can also be referred to as strains\,) that are able to co-exist within the host. Uncovering information about quasi-species populations of microbes significantly benefits the study of disease progression\, antiviral drug design\, vaccine design and viral pathogenesis. We present a new analysis pipeline called ViQuaS for viral quasispecies spectrum reconstruction using short next-generation sequencing reads. ViQuaS is based on a novel reference-assisted de novo assembly algorithm for constructing local haplotypes. URL:https://ibecbarcelona.eu/event/ibec-seminar-prof-saman-k-halgamuge/ CATEGORIES:IBEC Seminar END:VEVENT BEGIN:VEVENT DTSTART;TZID=Europe/Madrid:20150706T150000 DTEND;TZID=Europe/Madrid:20150706T160000 DTSTAMP:20260419T072517 CREATED:20150701T122222Z LAST-MODIFIED:20150701T122222Z UID:95860-1436194800-1436198400@ibecbarcelona.eu SUMMARY:IBEC Seminar: Prof. Saman K. Halgamuge DESCRIPTION:Big Data Analytics in Metagenomics\nSaman K. Halgamuge\, Melbourne School of Engineering\, University of Melbourne\, Australia\nIn collaboration with researchers in Academia Sinica and Metabolomics Australia/Department of Botany at Melbourne\, we have been working in two areas of Bioinformatics: Metabolomics focusing on microbes and Metagenomics focusing on plants. Profiling large sets of data resulted from technological advances in whole genome sequencing and MALDI Imaging type technologies that can reveal vital information about the environment and plants\, which is our major or primary source of food on Earth. Recently we have demonstrated considerable success in using unsupervised clustering techniques to analyse genetic and metabolomic data. This includes analysis of viral quasi species\, metabolomics and microbial metagenomes. \nSome microbes in the environment appear to look very similar and found “living together” in communities in non-separable ways\, making them harder to culture in a lab. To make matters worst\, considering our belief\, if it is correct at all\, that we know only about up to 2% of the microbes around us. When we know only so little about the data labels\, in this case\, about the identity of the species. It is even more challenging to recognise patterns associated with the genomes of the quasispecies (a set of genetically related but non-identical viral mutant types\, which can also be referred to as strains\,) that are able to co-exist within the host. Uncovering information about quasi-species populations of microbes significantly benefits the study of disease progression\, antiviral drug design\, vaccine design and viral pathogenesis. We present a new analysis pipeline called ViQuaS for viral quasispecies spectrum reconstruction using short next-generation sequencing reads. ViQuaS is based on a novel reference-assisted de novo assembly algorithm for constructing local haplotypes. URL:https://ibecbarcelona.eu/event/ibec-seminar-prof-saman-k-halgamuge-2/ CATEGORIES:IBEC Seminar END:VEVENT BEGIN:VEVENT DTSTART;TZID=Europe/Madrid:20150724T100000 DTEND;TZID=Europe/Madrid:20150724T110000 DTSTAMP:20260419T072517 CREATED:20150720T092247Z LAST-MODIFIED:20150720T092247Z UID:95864-1437732000-1437735600@ibecbarcelona.eu SUMMARY:IBEC Seminar: Dr. François St. Pierre DESCRIPTION:Imaging electrical activity in vivo with ultrafast protein sensors \nDr. François St. Pierre\, Department of Neuroscience\, Baylor College of Medicine / \nDepartment of Electrical and Computer Engineering\, Rice University\nImaging of rapid brain electrical activity has been on the wish list of neurobiologists for many years and has received renewed attention with the launch of the BRAIN initiative by the White House in the U.S.A. In particular\, neuroscientists have long sought voltage sensors based on proteins to reveal brain activity in genetically defined neuronal circuits. I will present novel protein voltage sensors that leverage optimized parts and creative new designs to report neural activity with unprecedented temporal resolution in vitro\, in brain slices and in vivo. Importantly\, these synthetic sensors report neural activity at the millisecond timescale over which key information processing functions are thought to take place. I will show how these new optical tools enable us to follow neural information processing in the fly visual system with subcellular resolution. I will also present our recent success at monitoring spontaneous electrical activity in stem cell-derived cardiomyocytes. URL:https://ibecbarcelona.eu/event/ibec-seminar-dr-francois-st-pierre-2/ CATEGORIES:IBEC Seminar END:VEVENT BEGIN:VEVENT DTSTART;TZID=Europe/Madrid:20150724T100000 DTEND;TZID=Europe/Madrid:20150724T110000 DTSTAMP:20260419T072517 CREATED:20150720T092247Z LAST-MODIFIED:20150720T102616Z UID:18148-1437732000-1437735600@ibecbarcelona.eu SUMMARY:IBEC Seminar: Dr. François St. Pierre DESCRIPTION:Imaging electrical activity in vivo with ultrafast protein sensors \nDr. François St. Pierre\, Department of Neuroscience\, Baylor College of Medicine / \nDepartment of Electrical and Computer Engineering\, Rice University\nImaging of rapid brain electrical activity has been on the wish list of neurobiologists for many years and has received renewed attention with the launch of the BRAIN initiative by the White House in the U.S.A. In particular\, neuroscientists have long sought voltage sensors based on proteins to reveal brain activity in genetically defined neuronal circuits. I will present novel protein voltage sensors that leverage optimized parts and creative new designs to report neural activity with unprecedented temporal resolution in vitro\, in brain slices and in vivo. Importantly\, these synthetic sensors report neural activity at the millisecond timescale over which key information processing functions are thought to take place. I will show how these new optical tools enable us to follow neural information processing in the fly visual system with subcellular resolution. I will also present our recent success at monitoring spontaneous electrical activity in stem cell-derived cardiomyocytes. URL:https://ibecbarcelona.eu/event/ibec-seminar-dr-francois-st-pierre/ CATEGORIES:IBEC Seminar END:VEVENT BEGIN:VEVENT DTSTART;TZID=Europe/Madrid:20150917T100000 DTEND;TZID=Europe/Madrid:20150917T110000 DTSTAMP:20260419T072517 CREATED:20150803T122810Z LAST-MODIFIED:20150803T122810Z UID:95865-1442484000-1442487600@ibecbarcelona.eu SUMMARY:IBEC Seminar: Richard Reilly DESCRIPTION:All a question of Timing: Sensory processing in Dystonia and Parkinson’s Disease\nProfessor Richard Reilly\, Trinity Centre for Bioengineering · Trinity College Dublin\nThere are many challenges in the diagnosis and management of neurological disorders. Neural Engineering can help address some of these by the development of novel engineering\, computational and experimental methods to help understand the pathogenesis of neurological disorders. This talk will detail results from recent neural engineering studies into understanding cervical dystonia and Parkinson’s disease. \nWhile the pathogenesis of cervical dystonia remains unknown\, recent animal and clinical experimental studies have indicated its probable mechanisms. Human movement involves a complex series of coordinated musculoskeletal but also neural processes. A breakdown in any of these processes can result in irregular movement. The temporal discrimination threshold is the shortest time interval at which two sensory stimuli presented sequentially are detected as asynchronous by the observer. Studies in our group and that of others have shown that abnormal temporal discrimination is a mediational endophenotype of cervical dystonia and informs new concepts of disease pathogenesis. Our hypothesis is that both abnormal temporal discrimination and cervical dystonia are due to a disorder of the midbrain network for covert attentional orienting caused by reduced gamma-aminobutyric acid inhibition\, resulting from\, in turn\, from as yet undetermined\, genetic mutations. Such disinhibition is a) subclinically manifested by abnormal temporal discrimination due to prolonged duration firing of the visual sensory neurons in the superficial laminae of the superior colliculus\, b) clinically manifested by cervical dystonia due to disinhibited burst activity of the cephalomotor neurons of the intermediate and deep laminae of the superior colliculus. Abnormal temporal discrimination in unaffected first-degree relatives of patients with cervical dystonia represents a subclinical manifestation of defective gamma-aminobutyric acid activity both within the superior colliculus and from the substantia nigra pars reticulata. This talk will review some our recent experiments addressing this hypothesis. \nSensory and perceptual disturbances are common in Parkinson’s disease. Subtle deficits of the sensory system\, often not detected by routine examination\, occur in people with Parkinson’s disease. From simple anosmia and impaired kinesthetic perception\, to more complex visual hallucinations and spatiotemporal perceptual abnormalities\, altered sensory processing is found across multiple modalities. Of note\, integration of multiple environmental sensory inputs is crucial for a refined but complex goal-directed motor output (e.g. locomotion through a crowded environment). There is increasing evidence that these sensory deficits contribute to the pathophysiology of some of the abnormal motor features of Parkinson’s disease. This talk will review some of our recent experiments to probe multisensory deficits in Parkinson’s disease and introduce one intervention developed around sensory and cognitive processing. URL:https://ibecbarcelona.eu/event/ibec-seminar-richard-reilly-2/ CATEGORIES:IBEC Seminar END:VEVENT BEGIN:VEVENT DTSTART;TZID=Europe/Madrid:20150917T100000 DTEND;TZID=Europe/Madrid:20150917T110000 DTSTAMP:20260419T072517 CREATED:20150803T122810Z LAST-MODIFIED:20150803T122810Z UID:18430-1442484000-1442487600@ibecbarcelona.eu SUMMARY:IBEC Seminar: Richard Reilly DESCRIPTION:All a question of Timing: Sensory processing in Dystonia and Parkinson’s Disease\nProfessor Richard Reilly\, Trinity Centre for Bioengineering · Trinity College Dublin\nThere are many challenges in the diagnosis and management of neurological disorders. Neural Engineering can help address some of these by the development of novel engineering\, computational and experimental methods to help understand the pathogenesis of neurological disorders. This talk will detail results from recent neural engineering studies into understanding cervical dystonia and Parkinson’s disease. \nWhile the pathogenesis of cervical dystonia remains unknown\, recent animal and clinical experimental studies have indicated its probable mechanisms. Human movement involves a complex series of coordinated musculoskeletal but also neural processes. A breakdown in any of these processes can result in irregular movement. The temporal discrimination threshold is the shortest time interval at which two sensory stimuli presented sequentially are detected as asynchronous by the observer. Studies in our group and that of others have shown that abnormal temporal discrimination is a mediational endophenotype of cervical dystonia and informs new concepts of disease pathogenesis. Our hypothesis is that both abnormal temporal discrimination and cervical dystonia are due to a disorder of the midbrain network for covert attentional orienting caused by reduced gamma-aminobutyric acid inhibition\, resulting from\, in turn\, from as yet undetermined\, genetic mutations. Such disinhibition is a) subclinically manifested by abnormal temporal discrimination due to prolonged duration firing of the visual sensory neurons in the superficial laminae of the superior colliculus\, b) clinically manifested by cervical dystonia due to disinhibited burst activity of the cephalomotor neurons of the intermediate and deep laminae of the superior colliculus. Abnormal temporal discrimination in unaffected first-degree relatives of patients with cervical dystonia represents a subclinical manifestation of defective gamma-aminobutyric acid activity both within the superior colliculus and from the substantia nigra pars reticulata. This talk will review some our recent experiments addressing this hypothesis. \nSensory and perceptual disturbances are common in Parkinson’s disease. Subtle deficits of the sensory system\, often not detected by routine examination\, occur in people with Parkinson’s disease. From simple anosmia and impaired kinesthetic perception\, to more complex visual hallucinations and spatiotemporal perceptual abnormalities\, altered sensory processing is found across multiple modalities. Of note\, integration of multiple environmental sensory inputs is crucial for a refined but complex goal-directed motor output (e.g. locomotion through a crowded environment). There is increasing evidence that these sensory deficits contribute to the pathophysiology of some of the abnormal motor features of Parkinson’s disease. This talk will review some of our recent experiments to probe multisensory deficits in Parkinson’s disease and introduce one intervention developed around sensory and cognitive processing. URL:https://ibecbarcelona.eu/event/ibec-seminar-richard-reilly/ CATEGORIES:IBEC Seminar END:VEVENT BEGIN:VEVENT DTSTART;TZID=Europe/Madrid:20150918T100000 DTEND;TZID=Europe/Madrid:20150918T110000 DTSTAMP:20260419T072517 CREATED:20150629T072320Z LAST-MODIFIED:20150629T072320Z UID:95859-1442570400-1442574000@ibecbarcelona.eu SUMMARY:IBEC Seminar: Marc Martí-Renom DESCRIPTION:Structure determination of genomes and genomic domains by satisfaction of spatial restraints\nMarc Martí-Renom\, ICREA Research Professor / CNAG / CRG\nThe genome three-dimensional (3D) organization plays important\, yet poorly understood roles in gene regulation. Chromosomes assume multiple distinct conformations in relation to the expression status of resident genes and undergo dramatic alterations in higher order structure through the cell cycle. Despite advances in microscopy\, a general technique to determine the 3D conformation of chromatin has been lacking. We developed a new method for the determination of the 3D conformation of chromatin domains in the interphase nucleus\, which combines 3C-based experiments with the computational Integrative Modeling Platform (IMP). The general approach of our method\, which has been applied to study the 3D conformation of the alpha-globin domain in the human genome [1]\, the Caulobacter crescentus whole genome [2]\, and the dynamic response of Topologically Associating Domains (TADs) to hormone treatment in breast cancer cell lines [3]\, opens the field for comprehensive studies of the 3D conformation of chromosomal domains and contributes to a more complete characterization of genome regulation.\n[1] D. Baù et al. Nat Struct Mol Biol (2011) 18:107.\n[2] M.A. Umbarger\, et al. Molecular Cell (2011) 44:252\n[3] Le Dily\, et al. Genes & Dev (2014) 28:2151 URL:https://ibecbarcelona.eu/event/ibec-seminar-marc-marti-renom-2/ CATEGORIES:IBEC Seminar END:VEVENT BEGIN:VEVENT DTSTART;TZID=Europe/Madrid:20150918T100000 DTEND;TZID=Europe/Madrid:20150918T110000 DTSTAMP:20260419T072517 CREATED:20150629T072320Z LAST-MODIFIED:20150629T072320Z UID:17667-1442570400-1442574000@ibecbarcelona.eu SUMMARY:IBEC Seminar: Marc Martí-Renom DESCRIPTION:Structure determination of genomes and genomic domains by satisfaction of spatial restraints\nMarc Martí-Renom\, ICREA Research Professor / CNAG / CRG\nThe genome three-dimensional (3D) organization plays important\, yet poorly understood roles in gene regulation. Chromosomes assume multiple distinct conformations in relation to the expression status of resident genes and undergo dramatic alterations in higher order structure through the cell cycle. Despite advances in microscopy\, a general technique to determine the 3D conformation of chromatin has been lacking. We developed a new method for the determination of the 3D conformation of chromatin domains in the interphase nucleus\, which combines 3C-based experiments with the computational Integrative Modeling Platform (IMP). The general approach of our method\, which has been applied to study the 3D conformation of the alpha-globin domain in the human genome [1]\, the Caulobacter crescentus whole genome [2]\, and the dynamic response of Topologically Associating Domains (TADs) to hormone treatment in breast cancer cell lines [3]\, opens the field for comprehensive studies of the 3D conformation of chromosomal domains and contributes to a more complete characterization of genome regulation.\n[1] D. Baù et al. Nat Struct Mol Biol (2011) 18:107.\n[2] M.A. Umbarger\, et al. Molecular Cell (2011) 44:252\n[3] Le Dily\, et al. Genes & Dev (2014) 28:2151 URL:https://ibecbarcelona.eu/event/ibec-seminar-marc-marti-renom/ CATEGORIES:IBEC Seminar END:VEVENT BEGIN:VEVENT DTSTART;TZID=Europe/Madrid:20150922T000000 DTEND;TZID=Europe/Madrid:20150922T130000 DTSTAMP:20260419T072517 CREATED:20150918T102346Z LAST-MODIFIED:20150918T102346Z UID:18953-1442880000-1442926800@ibecbarcelona.eu SUMMARY:IBEC Seminar: Albert Folch DESCRIPTION:Print-and-Play Microfluidics\nAlbert Folch\, Associate Professor\, University of Washington\nBiologists and clinicians typically do not have access to microfluidic technology because they do not have the engineering expertise or equipment required to fabricate and/or operate microfluidic devices. Furthermore\, the present commercialization path for microfluidic devices is usually restricted to high-volume applications in order to recover the large investment needed to develop the plastic molding processes. We are developing microfluidic devices through stereolithography\, a form of 3D printing\, in order to make microfluidic technology readily available via the web to biomedical scientists. Our lab presently focuses on developing 3D-printable microdevices that facilitate the advancement of basic neuroscience and translational cancer applications. The lab’s long-term mission is to make microfluidic devices as easy to use as smartphones and make them easily available to clinicians in order to enable novel cancer diagnostics and therapies. URL:https://ibecbarcelona.eu/event/ibec-seminar-albert-folch/ CATEGORIES:IBEC Seminar END:VEVENT BEGIN:VEVENT DTSTART;TZID=Europe/Madrid:20150922T000000 DTEND;TZID=Europe/Madrid:20150922T130000 DTSTAMP:20260419T072517 CREATED:20150918T102346Z LAST-MODIFIED:20150918T102346Z UID:95868-1442880000-1442926800@ibecbarcelona.eu SUMMARY:IBEC Seminar: Albert Folch DESCRIPTION:Print-and-Play Microfluidics\nAlbert Folch\, Associate Professor\, University of Washington\nBiologists and clinicians typically do not have access to microfluidic technology because they do not have the engineering expertise or equipment required to fabricate and/or operate microfluidic devices. Furthermore\, the present commercialization path for microfluidic devices is usually restricted to high-volume applications in order to recover the large investment needed to develop the plastic molding processes. We are developing microfluidic devices through stereolithography\, a form of 3D printing\, in order to make microfluidic technology readily available via the web to biomedical scientists. Our lab presently focuses on developing 3D-printable microdevices that facilitate the advancement of basic neuroscience and translational cancer applications. The lab’s long-term mission is to make microfluidic devices as easy to use as smartphones and make them easily available to clinicians in order to enable novel cancer diagnostics and therapies. URL:https://ibecbarcelona.eu/event/ibec-seminar-albert-folch-2/ CATEGORIES:IBEC Seminar END:VEVENT BEGIN:VEVENT DTSTART;TZID=Europe/Madrid:20151016T100000 DTEND;TZID=Europe/Madrid:20151016T110000 DTSTAMP:20260419T072517 CREATED:20150713T060412Z LAST-MODIFIED:20150713T060412Z UID:95862-1444989600-1444993200@ibecbarcelona.eu SUMMARY:IBEC Seminar: Lorenzo Albertazzi DESCRIPTION:Nanoscopy for Nanomedicine: looking at nanomaterials in action one molecule at a time\nLorenzo Albertazzi\, IBEC\nThe use of nanomaterials for biomedical purposes such as drug or gene delivery is one of the key applications of nanotechnology. In this framework a variety of materials have been fabricated\, evaluated in cells and in vivo and\, in some cases\, successfully translated into clinical applications. A crucial factor limiting the design of effective nanocarriers is the lack of knowledge about materials-cell interactions that severely limit the rational design of nanosized devices.\nHere I’ll show how advanced optical microscopy techniques can shine a light on nanomaterials behavior in the biological environment\, helping us to understand structure-activity relationships and to design improved and more effective therapeutic devices. In particular I’ll discuss the ability of super resolution microscopy to image with nanometric resolution nanomaterials interactions with target cells. The potential and the applications of the technique will be discussed\, with particular emphasis on the study of self-assembled bio-inspired materials. URL:https://ibecbarcelona.eu/event/ibec-seminar-lorenzo-albertazzi-2/ CATEGORIES:IBEC Seminar END:VEVENT BEGIN:VEVENT DTSTART;TZID=Europe/Madrid:20151016T100000 DTEND;TZID=Europe/Madrid:20151016T110000 DTSTAMP:20260419T072517 CREATED:20150713T060412Z LAST-MODIFIED:20150713T060412Z UID:18096-1444989600-1444993200@ibecbarcelona.eu SUMMARY:IBEC Seminar: Lorenzo Albertazzi DESCRIPTION:Nanoscopy for Nanomedicine: looking at nanomaterials in action one molecule at a time\nLorenzo Albertazzi\, IBEC\nThe use of nanomaterials for biomedical purposes such as drug or gene delivery is one of the key applications of nanotechnology. In this framework a variety of materials have been fabricated\, evaluated in cells and in vivo and\, in some cases\, successfully translated into clinical applications. A crucial factor limiting the design of effective nanocarriers is the lack of knowledge about materials-cell interactions that severely limit the rational design of nanosized devices.\nHere I’ll show how advanced optical microscopy techniques can shine a light on nanomaterials behavior in the biological environment\, helping us to understand structure-activity relationships and to design improved and more effective therapeutic devices. In particular I’ll discuss the ability of super resolution microscopy to image with nanometric resolution nanomaterials interactions with target cells. The potential and the applications of the technique will be discussed\, with particular emphasis on the study of self-assembled bio-inspired materials. URL:https://ibecbarcelona.eu/event/ibec-seminar-lorenzo-albertazzi/ CATEGORIES:IBEC Seminar END:VEVENT BEGIN:VEVENT DTSTART;TZID=UTC:20151019T100000 DTEND;TZID=UTC:20151019T120000 DTSTAMP:20260419T072517 CREATED:20151006T054804Z LAST-MODIFIED:20151006T054804Z UID:95869-1445248800-1445256000@ibecbarcelona.eu SUMMARY:IBEC Seminar: Cell Imaging Day - Molecular Probes DESCRIPTION:Cell Imaging Day – Molecular Probes\nClara Streiff\, Senior Technical Specialist · Molecular Probes – ThermoFisher Scientific\nSeminar:\n• Marcaje de estructuras celulares en células vivas (Bacmam Cellight)\n• Proliferación celular (Click-iT® EdU)\n• Estrés oxidativo (CellROX®)\n• Fagocitosis (PHrodo)\n• Apoptosis (Cellevent Caspase 3 and 7)\n• Mejorando la señal fluorescente en células vivas y fijadas (ReadyProbes reactivos “ready to use”)\n• Ventajas de la citometría por focalización acústica (Attune®NxT acoustic focusing cytometer) \nAl final del seminario podrás pasar tus muestras en el microscopio EVOS® FL AUTO y prueba nuestros reactivos ReadyProbes de Molecular Probes sin compromiso\, reactivos para imaging que permiten marcar tus células sin necesidad de hacer calculos\, diluciones ni pipetear. URL:https://ibecbarcelona.eu/event/ibec-seminar-cell-imaging-day-molecular-probes-2/ CATEGORIES:IBEC Seminar END:VEVENT BEGIN:VEVENT DTSTART;TZID=UTC:20151019T100000 DTEND;TZID=UTC:20151019T120000 DTSTAMP:20260419T072517 CREATED:20151006T054804Z LAST-MODIFIED:20151006T054804Z UID:19129-1445248800-1445256000@ibecbarcelona.eu SUMMARY:IBEC Seminar: Cell Imaging Day - Molecular Probes DESCRIPTION:Cell Imaging Day – Molecular Probes\nClara Streiff\, Senior Technical Specialist · Molecular Probes – ThermoFisher Scientific\nSeminar:\n• Marcaje de estructuras celulares en células vivas (Bacmam Cellight)\n• Proliferación celular (Click-iT® EdU)\n• Estrés oxidativo (CellROX®)\n• Fagocitosis (PHrodo)\n• Apoptosis (Cellevent Caspase 3 and 7)\n• Mejorando la señal fluorescente en células vivas y fijadas (ReadyProbes reactivos “ready to use”)\n• Ventajas de la citometría por focalización acústica (Attune®NxT acoustic focusing cytometer) \nAl final del seminario podrás pasar tus muestras en el microscopio EVOS® FL AUTO y prueba nuestros reactivos ReadyProbes de Molecular Probes sin compromiso\, reactivos para imaging que permiten marcar tus células sin necesidad de hacer calculos\, diluciones ni pipetear. URL:https://ibecbarcelona.eu/event/ibec-seminar-cell-imaging-day-molecular-probes/ CATEGORIES:IBEC Seminar END:VEVENT BEGIN:VEVENT DTSTART;TZID=Europe/Madrid:20151023T100000 DTEND;TZID=Europe/Madrid:20151023T110000 DTSTAMP:20260419T072517 CREATED:20150803T123423Z LAST-MODIFIED:20150803T123423Z UID:18434-1445594400-1445598000@ibecbarcelona.eu SUMMARY:IBEC Seminar: Miquel Bosch Pita DESCRIPTION:The molecular mechanisms of memory persistence: imaging how single synapses learn in real time\nMiquel Bosch Pita\, Nanoprobes and nanoswitches group\, IBEC\nMemories are stored in our brain through the ability of synaptic connections to modify their structure and function in a long-lasting way. However\, nobody has ever observed how these changes occur in a single synapse in real time.\nI will explain how we used a new combination of optical technologies to reveal the molecular remodeling that takes place inside a synapse during the creation of a memory. We used two-photon microscopy to stimulate individual synapses and to visualize protein trafficking in real time. We identified a unique protein that is rapidly and persistently captured in potentiated synapses\, forming a new macromolecule that could serve as a memory tag. We developed a novel photo-marking technique that allowed us to localize the same synapses under both two-photon and electron microscopies. This way we observed how different synaptic structures evolve asynchronously in three temporal phases during synaptic potentiation. URL:https://ibecbarcelona.eu/event/ibec-seminar-miquel-bosch-pita/ CATEGORIES:IBEC Seminar END:VEVENT BEGIN:VEVENT DTSTART;TZID=Europe/Madrid:20151023T100000 DTEND;TZID=Europe/Madrid:20151023T110000 DTSTAMP:20260419T072517 CREATED:20150803T123423Z LAST-MODIFIED:20150803T123423Z UID:95866-1445594400-1445598000@ibecbarcelona.eu SUMMARY:IBEC Seminar: Miquel Bosch Pita DESCRIPTION:The molecular mechanisms of memory persistence: imaging how single synapses learn in real time\nMiquel Bosch Pita\, Nanoprobes and nanoswitches group\, IBEC\nMemories are stored in our brain through the ability of synaptic connections to modify their structure and function in a long-lasting way. However\, nobody has ever observed how these changes occur in a single synapse in real time.\nI will explain how we used a new combination of optical technologies to reveal the molecular remodeling that takes place inside a synapse during the creation of a memory. We used two-photon microscopy to stimulate individual synapses and to visualize protein trafficking in real time. We identified a unique protein that is rapidly and persistently captured in potentiated synapses\, forming a new macromolecule that could serve as a memory tag. We developed a novel photo-marking technique that allowed us to localize the same synapses under both two-photon and electron microscopies. This way we observed how different synaptic structures evolve asynchronously in three temporal phases during synaptic potentiation. URL:https://ibecbarcelona.eu/event/ibec-seminar-miquel-bosch-pita-2/ CATEGORIES:IBEC Seminar END:VEVENT END:VCALENDAR