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BEGIN:VEVENT
DTSTART;TZID=Europe/Madrid:20170714T100000
DTEND;TZID=Europe/Madrid:20170714T110000
DTSTAMP:20260405T213701
CREATED:20170630T073735Z
LAST-MODIFIED:20170630T073817Z
UID:30091-1500026400-1500030000@ibecbarcelona.eu
SUMMARY:IBEC Seminar: Maria Vinaixa
DESCRIPTION:Mass spectrometry and metabolomics data analysis for synthetic biology\n Maria Vinaixa\, Synthetic Biology for Fine and Speciality Chemicals (SYNBIOCHEM)\, Manchester Institute of Biotechnology\nSynthetic biology builds upon the creation of new biologically inspired standardized parts that can be put together using design or simulations tools to build circuits that will create de-novo biological functions or modify existing ones. Using synthetic biology\, microbial cell factories can be engineered to provide new sustainable bio-routes for the production of fuels\, biopharmaceuticals\, fragrances\, and food flavors among others. In this regard\, the SYNBIOCHEM Centre (www.synbiochem.co.uk) has set-up an automated Design/Build/Test/Learn pipeline designed to provide access to target fine chemicals through iterative\, rapid and predictable engineering of production pathways and microbial strains. This pipeline moves from Design of new parts (e.g. enzymes\, regulatory circuits\, metabolic pathways)\, through to combinatorial high-throughput Build approaches (directed evolution\, components\, pathways and strain assembly) and high-throughput analytics in Test (product extraction\, instrumental analysis\, data analysis and sharing) feeding back to improved designs via an active Learning stage at each cycle iteration. This pipeline allows unprecedented possibilities for retro biosynthesis of non-natural products and for the expansion of natural products chemical diversity. Screening for the small-molecule structure diversity emanating from such pipeline is an analytically daunting challenge. In this regard\, mass spectrometry (MS) is a key analytical technology offering the high throughput screening capabilities as well as the versatility needed to cope with such chemical diversity. However\, curating MS data and merging it with all other types of data generated through iterative D/B/T/L cycle so that it can be used to learn and redesign remains a challenge. Despite Metabolomics has powered computational solutions for MS data analysis; such solutions do only partially cover the needs within a synthetic biology context. Thus\, we are building the next generation computational toolbox for MS data analysis and storage so it can be harvested across the entire pipeline. In this seminar\, main capabilities and functionalities on such toolbox are going to be discussed.
URL:https://ibecbarcelona.eu/event/ibec-seminar-maria-vinaixa/
LOCATION:IBEC\, floor 11\, Tower I\, Baldiri Reixac 4-8\, 08028 Barcelona\, Spain
CATEGORIES:IBEC Seminar
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Europe/Madrid:20170714T100000
DTEND;TZID=Europe/Madrid:20170714T110000
DTSTAMP:20260405T213701
CREATED:20170630T073735Z
LAST-MODIFIED:20170630T073735Z
UID:96075-1500026400-1500030000@ibecbarcelona.eu
SUMMARY:IBEC Seminar: Maria Vinaixa
DESCRIPTION:Mass spectrometry and metabolomics data analysis for synthetic biology\n Maria Vinaixa\, Synthetic Biology for Fine and Speciality Chemicals (SYNBIOCHEM)\, Manchester Institute of Biotechnology\nSynthetic biology builds upon the creation of new biologically inspired standardized parts that can be put together using design or simulations tools to build circuits that will create de-novo biological functions or modify existing ones. Using synthetic biology\, microbial cell factories can be engineered to provide new sustainable bio-routes for the production of fuels\, biopharmaceuticals\, fragrances\, and food flavors among others. In this regard\, the SYNBIOCHEM Centre (www.synbiochem.co.uk) has set-up an automated Design/Build/Test/Learn pipeline designed to provide access to target fine chemicals through iterative\, rapid and predictable engineering of production pathways and microbial strains. This pipeline moves from Design of new parts (e.g. enzymes\, regulatory circuits\, metabolic pathways)\, through to combinatorial high-throughput Build approaches (directed evolution\, components\, pathways and strain assembly) and high-throughput analytics in Test (product extraction\, instrumental analysis\, data analysis and sharing) feeding back to improved designs via an active Learning stage at each cycle iteration. This pipeline allows unprecedented possibilities for retro biosynthesis of non-natural products and for the expansion of natural products chemical diversity. Screening for the small-molecule structure diversity emanating from such pipeline is an analytically daunting challenge. In this regard\, mass spectrometry (MS) is a key analytical technology offering the high throughput screening capabilities as well as the versatility needed to cope with such chemical diversity. However\, curating MS data and merging it with all other types of data generated through iterative D/B/T/L cycle so that it can be used to learn and redesign remains a challenge. Despite Metabolomics has powered computational solutions for MS data analysis; such solutions do only partially cover the needs within a synthetic biology context. Thus\, we are building the next generation computational toolbox for MS data analysis and storage so it can be harvested across the entire pipeline. In this seminar\, main capabilities and functionalities on such toolbox are going to be discussed.
URL:https://ibecbarcelona.eu/event/ibec-seminar-maria-vinaixa-2/
LOCATION:IBEC\, floor 11\, Tower I\, Baldiri Reixac 4-8\, 08028 Barcelona\, Spain
CATEGORIES:IBEC Seminar
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Europe/Madrid:20170725T100000
DTEND;TZID=Europe/Madrid:20170725T110000
DTSTAMP:20260405T213701
CREATED:20170717T120502Z
LAST-MODIFIED:20170717T120502Z
UID:96085-1500976800-1500980400@ibecbarcelona.eu
SUMMARY:IBEC Seminar: Ronen Zaidel-Bar
DESCRIPTION:Regulation of actomyosin contractility in C. elegans\nRonen Zaidel-Bar\, Mechanobiology Institute Singapore and Tel-Aviv University Medical School\nThe actomyosin cortex is responsible for cell shape and for dynamic processes such as cell polarization and cytokinesis. We are studying the regulation of cortical contractility in the C. elegans zygote\, using genetic loss of function and live-imaging. In my talk\, I will discuss recent findings regarding two proteins: the actin cross-linking protein plastin (PLST-1) and the transmembrane receptor E-cadherin (HMR-1). Consistent with previous in-vitro reconstitution studies\, we show that an optimal level of cross-linking by plastin is required for the generation of coordinated long-range contractions in the cortex; without the connectivity afforded by plastin\, zygote polarization and cytokinesis are severely perturbed. E-cadherin is well known for its role as a cell-cell adhesion receptor. \nSurprisingly\, we discovered a role for non-junctional E-cadherin clusters in regulating cortical contractility. E-cadherin clusters inhibit RhoA and non-muscle myosin II activity at the cortex and form a physical barrier that slows actin flows. In the absence of non-junctional E-cadherin cytokinesis proceeds faster\, but the cortex is also at a risk of detaching from the plasma membrane. Thus\, our studies in the C. elegans zygote are shedding light on structural and regulatory mechanisms underlying cortex function.
URL:https://ibecbarcelona.eu/event/ibec-seminar-ronen-zaidel-bar-2/
LOCATION:IBEC\, floor 11\, Tower I\, Baldiri Reixac 4-8\, 08028 Barcelona\, Spain
CATEGORIES:IBEC Seminar
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Europe/Madrid:20170725T100000
DTEND;TZID=Europe/Madrid:20170725T110000
DTSTAMP:20260405T213701
CREATED:20170717T120502Z
LAST-MODIFIED:20170717T120502Z
UID:30583-1500976800-1500980400@ibecbarcelona.eu
SUMMARY:IBEC Seminar: Ronen Zaidel-Bar
DESCRIPTION:Regulation of actomyosin contractility in C. elegans\nRonen Zaidel-Bar\, Mechanobiology Institute Singapore and Tel-Aviv University Medical School\nThe actomyosin cortex is responsible for cell shape and for dynamic processes such as cell polarization and cytokinesis. We are studying the regulation of cortical contractility in the C. elegans zygote\, using genetic loss of function and live-imaging. In my talk\, I will discuss recent findings regarding two proteins: the actin cross-linking protein plastin (PLST-1) and the transmembrane receptor E-cadherin (HMR-1). Consistent with previous in-vitro reconstitution studies\, we show that an optimal level of cross-linking by plastin is required for the generation of coordinated long-range contractions in the cortex; without the connectivity afforded by plastin\, zygote polarization and cytokinesis are severely perturbed. E-cadherin is well known for its role as a cell-cell adhesion receptor. \nSurprisingly\, we discovered a role for non-junctional E-cadherin clusters in regulating cortical contractility. E-cadherin clusters inhibit RhoA and non-muscle myosin II activity at the cortex and form a physical barrier that slows actin flows. In the absence of non-junctional E-cadherin cytokinesis proceeds faster\, but the cortex is also at a risk of detaching from the plasma membrane. Thus\, our studies in the C. elegans zygote are shedding light on structural and regulatory mechanisms underlying cortex function.
URL:https://ibecbarcelona.eu/event/ibec-seminar-ronen-zaidel-bar/
LOCATION:IBEC\, floor 11\, Tower I\, Baldiri Reixac 4-8\, 08028 Barcelona\, Spain
CATEGORIES:IBEC Seminar
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Europe/Madrid:20170928T143000
DTEND;TZID=Europe/Madrid:20170928T153000
DTSTAMP:20260405T213701
CREATED:20170922T075539Z
LAST-MODIFIED:20170922T075539Z
UID:96099-1506609000-1506612600@ibecbarcelona.eu
SUMMARY:IBEC Seminar: Silvain Muller\, RegenHu
DESCRIPTION:Bioprinting Software for the RegenHU 3DBioprinting System\nSilvain Muller\, RegenHu\nBioprinting is a dynamic and exciting stage of evolution where\, recently\, considerable progress has been accomplished globally in the field of tissue engineering and bioprinting. These developments have in turn led to ground-breaking advancements in Bioprinting leading from fundamental research to Applied research and Clinical testing. Key components in the successful transition of this evolution are the software tools specifically designed to enable interaction between the hardware\, the biology and the users. \nDuring this seminar Silvain Muller from RegenHu will describe the capabilities of our 3D Bioprinter (3D Discovery) and will perform a presentation of the software package associated that that enables the design of complex structures and scaffolds and the transformation of DICOM images to 3D-bioprinted constructs.
URL:https://ibecbarcelona.eu/event/ibec-seminar-silvain-muller-regenhu-2/
LOCATION:IBEC\, floor 11\, Tower I\, Baldiri Reixac 4-8\, 08028 Barcelona\, Spain
CATEGORIES:IBEC Seminar
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Europe/Madrid:20170928T143000
DTEND;TZID=Europe/Madrid:20170928T153000
DTSTAMP:20260405T213701
CREATED:20170922T075539Z
LAST-MODIFIED:20170922T075807Z
UID:31465-1506609000-1506612600@ibecbarcelona.eu
SUMMARY:IBEC Seminar: Silvain Muller\, RegenHu
DESCRIPTION:Bioprinting Software for the RegenHU 3DBioprinting System\nSilvain Muller\, RegenHu\nBioprinting is a dynamic and exciting stage of evolution where\, recently\, considerable progress has been accomplished globally in the field of tissue engineering and bioprinting. These developments have in turn led to ground-breaking advancements in Bioprinting leading from fundamental research to Applied research and Clinical testing. Key components in the successful transition of this evolution are the software tools specifically designed to enable interaction between the hardware\, the biology and the users. \nDuring this seminar Silvain Muller from RegenHu will describe the capabilities of our 3D Bioprinter (3D Discovery) and will perform a presentation of the software package associated that that enables the design of complex structures and scaffolds and the transformation of DICOM images to 3D-bioprinted constructs.
URL:https://ibecbarcelona.eu/event/ibec-seminar-silvain-muller-regenhu/
LOCATION:IBEC\, floor 11\, Tower I\, Baldiri Reixac 4-8\, 08028 Barcelona\, Spain
CATEGORIES:IBEC Seminar
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Europe/Madrid:20171006T100000
DTEND;TZID=Europe/Madrid:20171006T110000
DTSTAMP:20260405T213701
CREATED:20170926T091948Z
LAST-MODIFIED:20170926T091948Z
UID:96102-1507284000-1507287600@ibecbarcelona.eu
SUMMARY:IBEC Seminar: Fabio Variola
DESCRIPTION:At the intersection of biomaterials\, surface science and medicine\nFabio Variola\, Associate Professor\, Biomedical Engineering\, Cellular and Molecular Medicine\, University of Ottawa\nIn the quest for the next generation of functional biomaterials and new solutions in health-related research\, investigators have sought inspiration from nature by developing better performing bio-derived materials (e.g. collagen\, chitosan)\, reproducing naturally occurring micro and nanostructures (e.g. nanoporosity of collagen-apatite interfaces in bone\, ECM nanotopography) and devising strategies that mimic naturally occurring phenomena (e.g. mussel attachment). In this context\, our team has employed bio-derived materials and bio-inspired structures towards the creation of platforms and interfaces to investigate and control cellular events. In particular\, we successfully reproduced a bioactive nanoporosity on titanium\, the gold standard in medicine\, by simple chemical and electrochemical methods\, capable of positively affecting cell activity providing antibacterial properties. Anodization permitted not only to create semiordered nanotubular surfaces which can be tuned in terms of diameter and spacing\, but also a nanometric 3-dimensional hierarchical surface that mimics that of biologically successful life forms such as diatoms. Moreover\, we are currently working on understanding the effects on cells of poly(dopamine)\, an adhesive polymer derived from mussels\, as a multifunctional layer for direct cueing to cells. In parallel\, our team has also contributed to the development of collagen- and chitosan-based materials for applications ranging from cardiac and neuronal tissue engineering to synthetic blood vessels and drug delivery platforms.
URL:https://ibecbarcelona.eu/event/ibec-seminar-fabio-variola-2/
LOCATION:IBEC\, floor 11\, Tower I\, Baldiri Reixac 4-8\, 08028 Barcelona\, Spain
CATEGORIES:IBEC Seminar
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Europe/Madrid:20171006T100000
DTEND;TZID=Europe/Madrid:20171006T110000
DTSTAMP:20260405T213701
CREATED:20170926T091948Z
LAST-MODIFIED:20170926T091948Z
UID:31487-1507284000-1507287600@ibecbarcelona.eu
SUMMARY:IBEC Seminar: Fabio Variola
DESCRIPTION:At the intersection of biomaterials\, surface science and medicine\nFabio Variola\, Associate Professor\, Biomedical Engineering\, Cellular and Molecular Medicine\, University of Ottawa\nIn the quest for the next generation of functional biomaterials and new solutions in health-related research\, investigators have sought inspiration from nature by developing better performing bio-derived materials (e.g. collagen\, chitosan)\, reproducing naturally occurring micro and nanostructures (e.g. nanoporosity of collagen-apatite interfaces in bone\, ECM nanotopography) and devising strategies that mimic naturally occurring phenomena (e.g. mussel attachment). In this context\, our team has employed bio-derived materials and bio-inspired structures towards the creation of platforms and interfaces to investigate and control cellular events. In particular\, we successfully reproduced a bioactive nanoporosity on titanium\, the gold standard in medicine\, by simple chemical and electrochemical methods\, capable of positively affecting cell activity providing antibacterial properties. Anodization permitted not only to create semiordered nanotubular surfaces which can be tuned in terms of diameter and spacing\, but also a nanometric 3-dimensional hierarchical surface that mimics that of biologically successful life forms such as diatoms. Moreover\, we are currently working on understanding the effects on cells of poly(dopamine)\, an adhesive polymer derived from mussels\, as a multifunctional layer for direct cueing to cells. In parallel\, our team has also contributed to the development of collagen- and chitosan-based materials for applications ranging from cardiac and neuronal tissue engineering to synthetic blood vessels and drug delivery platforms.
URL:https://ibecbarcelona.eu/event/ibec-seminar-fabio-variola/
LOCATION:IBEC\, floor 11\, Tower I\, Baldiri Reixac 4-8\, 08028 Barcelona\, Spain
CATEGORIES:IBEC Seminar
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Europe/Madrid:20171011T090000
DTEND;TZID=Europe/Madrid:20171011T130000
DTSTAMP:20260405T213701
CREATED:20171006T095729Z
LAST-MODIFIED:20171006T095729Z
UID:96110-1507712400-1507726800@ibecbarcelona.eu
SUMMARY:Extra IBEC Seminar: Multimode Reader User Day
DESCRIPTION:Multimode Reader User Day\nStephan Haberstock\, Detection Specialist\, Tecan\nCore Facilities would like to invite you to a user day for multimode microplate readers. \nThe programme will offer practical insights on the following topics:\n• Introduction to the basic detection modes (Absorbance\, Fluorescence\, Luminescence) and advanced assays like FRET\, TRF\, and TR-FRET\n• Infinite 200 Pro\, a multimode reader highly configurable.\n• Spark®\, the new multimode readers from Tecan & application based configurations for: \no cell assays\no fluorescence assays\no luminescence assays\no nucleic acid analysis \nAfter the presentations we are happy to answer your specific questions & help you optimizing your personal methods. And\, obviously\, you’ll be able to test our readers on site!
URL:https://ibecbarcelona.eu/event/extra-ibec-seminar-multimode-reader-user-day-2/
LOCATION:IBEC\, floor 11\, Tower I\, Baldiri Reixac 4-8\, 08028 Barcelona\, Spain
CATEGORIES:IBEC Seminar
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Europe/Madrid:20171011T090000
DTEND;TZID=Europe/Madrid:20171011T130000
DTSTAMP:20260405T213701
CREATED:20171006T095729Z
LAST-MODIFIED:20171006T095729Z
UID:38142-1507712400-1507726800@ibecbarcelona.eu
SUMMARY:Extra IBEC Seminar: Multimode Reader User Day
DESCRIPTION:Multimode Reader User Day\nStephan Haberstock\, Detection Specialist\, Tecan\nCore Facilities would like to invite you to a user day for multimode microplate readers. \nThe programme will offer practical insights on the following topics:\n• Introduction to the basic detection modes (Absorbance\, Fluorescence\, Luminescence) and advanced assays like FRET\, TRF\, and TR-FRET\n• Infinite 200 Pro\, a multimode reader highly configurable.\n• Spark®\, the new multimode readers from Tecan & application based configurations for: \no cell assays\no fluorescence assays\no luminescence assays\no nucleic acid analysis \nAfter the presentations we are happy to answer your specific questions & help you optimizing your personal methods. And\, obviously\, you’ll be able to test our readers on site!
URL:https://ibecbarcelona.eu/event/extra-ibec-seminar-multimode-reader-user-day/
LOCATION:IBEC\, floor 11\, Tower I\, Baldiri Reixac 4-8\, 08028 Barcelona\, Spain
CATEGORIES:IBEC Seminar
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Europe/Madrid:20171018T100000
DTEND;TZID=Europe/Madrid:20171018T110000
DTSTAMP:20260405T213701
CREATED:20170926T130337Z
LAST-MODIFIED:20170926T130337Z
UID:96106-1508320800-1508324400@ibecbarcelona.eu
SUMMARY:IBEC Seminar: Arpita Upadhyaya
DESCRIPTION:Push\, pull and sense: Forces and mechanosensing in immune cells\nArpita Upadhyaya\, Associate Professor\, Department of Physics\, IPST\, University of Maryland\nThe activation of lymphocytes is an essential step in the adaptive immune response. Lymphocyte activation involves the binding of specialized receptors (TCR in T cells and BCR in B cells) with antigen on the surface of antigen presenting cells. This leads to changes in cell morphology and the movement and assembly of receptors\, scaffold proteins and enzymes into signaling microclusters\, which are essential for immune cell activation. During this process\, cells of the immune system interact with structures that possess a diverse range of physical properties. I will summarize our recent studies from a biophysical perspective that examine how T cells and B cells respond to physical cues such as stiffness\, topography and ligand mobility. Specifically\, I will highlight the distinct roles of the actin and microtubule cytoskeleton in the exertion of mechanical stresses that support signaling activation\, microcluster assembly and receptor movement in T and B lymphocytes.
URL:https://ibecbarcelona.eu/event/ibec-seminar-arpita-upadhyaya-2/
LOCATION:IBEC\, floor 11\, Tower I\, Baldiri Reixac 4-8\, 08028 Barcelona\, Spain
CATEGORIES:IBEC Seminar
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Europe/Madrid:20171018T100000
DTEND;TZID=Europe/Madrid:20171018T110000
DTSTAMP:20260405T213701
CREATED:20170926T130337Z
LAST-MODIFIED:20170926T130337Z
UID:31495-1508320800-1508324400@ibecbarcelona.eu
SUMMARY:IBEC Seminar: Arpita Upadhyaya
DESCRIPTION:Push\, pull and sense: Forces and mechanosensing in immune cells\nArpita Upadhyaya\, Associate Professor\, Department of Physics\, IPST\, University of Maryland\nThe activation of lymphocytes is an essential step in the adaptive immune response. Lymphocyte activation involves the binding of specialized receptors (TCR in T cells and BCR in B cells) with antigen on the surface of antigen presenting cells. This leads to changes in cell morphology and the movement and assembly of receptors\, scaffold proteins and enzymes into signaling microclusters\, which are essential for immune cell activation. During this process\, cells of the immune system interact with structures that possess a diverse range of physical properties. I will summarize our recent studies from a biophysical perspective that examine how T cells and B cells respond to physical cues such as stiffness\, topography and ligand mobility. Specifically\, I will highlight the distinct roles of the actin and microtubule cytoskeleton in the exertion of mechanical stresses that support signaling activation\, microcluster assembly and receptor movement in T and B lymphocytes.
URL:https://ibecbarcelona.eu/event/ibec-seminar-arpita-upadhyaya/
LOCATION:IBEC\, floor 11\, Tower I\, Baldiri Reixac 4-8\, 08028 Barcelona\, Spain
CATEGORIES:IBEC Seminar
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Europe/Madrid:20171218T120000
DTEND;TZID=Europe/Madrid:20171218T130000
DTSTAMP:20260405T213701
CREATED:20171123T104034Z
LAST-MODIFIED:20171123T104034Z
UID:96138-1513598400-1513602000@ibecbarcelona.eu
SUMMARY:IBEC Seminar: Jonel Trebicka
DESCRIPTION:Liver fibrosis and Portal hypertension\nProf. Jonel Trebicka\, Laboratory for Liver Fibrosis and Portal Hypertension\, Dept of Internal Medicine\, University of Bonn\nIn patients with chronic liver disease\, portal hypertension and progressive fibrosis are concomitant pathological processes interacting with each-other and leading to severe complications. The mechanics of matrix and the distinct response of different hepatic cell types contribute in the development of liver injury and cancer. Moreover\, cellular mechanics play a crucial role on the remodeling of matrix in chronic liver disease. Interruption of the liver injury either by treating the initial liver injury and addressing the perpetuating risk factors will improve both fibrosis and prevent or ameliorate portal hypertension. Currently\, after the successful cure of viral hepatitis\, lifestyle-related liver damage due to chronic alcoholism or morbid obesity will remain the main factor leading to liver fibrosis and portal hypertension. Even though\, the basic pathogenetic mechanisms of development of fibrosis and portal hypertension are similar. Especially RhoA/Rho-kinase pathway is crucially involved in the pathogenetic processes inside and outside the liver. RhoA/Rho-kinase is crucial in mechanics of cells\, and the modulation of these targets has been evaluated in different animal models. Also\, some well-established drugs\, which are used in humans for other indications (for example\, statins)\, are promising if applied early and concomitantly to standard therapy. In the future\, more cell-specific targeting and personalized strategies must be considered to avoid progression of disease and complications.
URL:https://ibecbarcelona.eu/event/ibec-seminar-jonel-trebicka-4/
LOCATION:IBEC\, floor 11\, Tower I\, Baldiri Reixac 4-8\, 08028 Barcelona\, Spain
CATEGORIES:IBEC Seminar
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Europe/Madrid:20171218T120000
DTEND;TZID=Europe/Madrid:20171218T130000
DTSTAMP:20260405T213701
CREATED:20171123T104034Z
LAST-MODIFIED:20171127T103555Z
UID:56469-1513598400-1513602000@ibecbarcelona.eu
SUMMARY:IBEC Seminar: Jonel Trebicka
DESCRIPTION:Liver fibrosis and Portal hypertension\nProf. Jonel Trebicka\, Laboratory for Liver Fibrosis and Portal Hypertension\, Dept of Internal Medicine\, University of Bonn\nIn patients with chronic liver disease\, portal hypertension and progressive fibrosis are concomitant pathological processes interacting with each-other and leading to severe complications. The mechanics of matrix and the distinct response of different hepatic cell types contribute in the development of liver injury and cancer. Moreover\, cellular mechanics play a crucial role on the remodeling of matrix in chronic liver disease. Interruption of the liver injury either by treating the initial liver injury and addressing the perpetuating risk factors will improve both fibrosis and prevent or ameliorate portal hypertension. Currently\, after the successful cure of viral hepatitis\, lifestyle-related liver damage due to chronic alcoholism or morbid obesity will remain the main factor leading to liver fibrosis and portal hypertension. Even though\, the basic pathogenetic mechanisms of development of fibrosis and portal hypertension are similar. Especially RhoA/Rho-kinase pathway is crucially involved in the pathogenetic processes inside and outside the liver. RhoA/Rho-kinase is crucial in mechanics of cells\, and the modulation of these targets has been evaluated in different animal models. Also\, some well-established drugs\, which are used in humans for other indications (for example\, statins)\, are promising if applied early and concomitantly to standard therapy. In the future\, more cell-specific targeting and personalized strategies must be considered to avoid progression of disease and complications.
URL:https://ibecbarcelona.eu/event/ibec-seminar-jonel-trebicka/
LOCATION:IBEC\, floor 11\, Tower I\, Baldiri Reixac 4-8\, 08028 Barcelona\, Spain
CATEGORIES:IBEC Seminar
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Europe/Madrid:20180112T100000
DTEND;TZID=Europe/Madrid:20180112T110000
DTSTAMP:20260405T213701
CREATED:20171228T101943Z
LAST-MODIFIED:20171228T101943Z
UID:96148-1515751200-1515754800@ibecbarcelona.eu
SUMMARY:IBEC Seminar: Dong-Pyo Kim
DESCRIPTION:Advances in Microfluidic Technology Driven by Materials\nDong-Pyo Kim\, POSTECH (Pohang University of Science & Technology)\, Korea\nAdvanced microreaction technologies have been achieved the best by chemistry and engineering together\, rather than either alone. This talk shows typical cases of innovative microreactor systems and process intensification by adopting multifunctional phenomena of materials and the specialty as well as by embracing newly emerging fabrication methods. \nDong-Pyo Kim is a professor of POSTECH and director of Center for Intelligent Microprocess of Pharmaceutical Synthesis. He obtained PhD in Chemistry at Temple University in 1991\, then post-doctoral research in University of Illinois at Urbana-Champaign (Materials S.E.)\, and came to Korea Research Institute of Chemical Technology as a senior researcher. Prior to POSTECH at 2012\, he had worked in Applied Chemistry at Chungnam National University for 17 years. His research has been based on materials chemistry of silicon-based resin. Since 2004\, he has devoted to a microreaction field\, currently covered the reactor design\, fabrication as well as continuous-flow syntheses in organics\, polymers and nanomaterials. He has published > 250 peer-reviewed papers and 30 patents. He received Academic Excellence Award by Korean Chemical Society (2017)\, POSTECHian Scientist of the Year (2016)\, The Great Scientist Award (2016) and Best 100 Scientific Achievement (2014\, 2007) by National Research Foundation.
URL:https://ibecbarcelona.eu/event/ibec-seminar-dong-pyo-kim-2/
LOCATION:IBEC\, floor 11\, Tower I\, Baldiri Reixac 4-8\, 08028 Barcelona\, Spain
CATEGORIES:IBEC Seminar
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Europe/Madrid:20180112T100000
DTEND;TZID=Europe/Madrid:20180112T110000
DTSTAMP:20260405T213701
CREATED:20171228T101943Z
LAST-MODIFIED:20171228T101943Z
UID:57061-1515751200-1515754800@ibecbarcelona.eu
SUMMARY:IBEC Seminar: Dong-Pyo Kim
DESCRIPTION:Advances in Microfluidic Technology Driven by Materials\nDong-Pyo Kim\, POSTECH (Pohang University of Science & Technology)\, Korea\nAdvanced microreaction technologies have been achieved the best by chemistry and engineering together\, rather than either alone. This talk shows typical cases of innovative microreactor systems and process intensification by adopting multifunctional phenomena of materials and the specialty as well as by embracing newly emerging fabrication methods. \nDong-Pyo Kim is a professor of POSTECH and director of Center for Intelligent Microprocess of Pharmaceutical Synthesis. He obtained PhD in Chemistry at Temple University in 1991\, then post-doctoral research in University of Illinois at Urbana-Champaign (Materials S.E.)\, and came to Korea Research Institute of Chemical Technology as a senior researcher. Prior to POSTECH at 2012\, he had worked in Applied Chemistry at Chungnam National University for 17 years. His research has been based on materials chemistry of silicon-based resin. Since 2004\, he has devoted to a microreaction field\, currently covered the reactor design\, fabrication as well as continuous-flow syntheses in organics\, polymers and nanomaterials. He has published > 250 peer-reviewed papers and 30 patents. He received Academic Excellence Award by Korean Chemical Society (2017)\, POSTECHian Scientist of the Year (2016)\, The Great Scientist Award (2016) and Best 100 Scientific Achievement (2014\, 2007) by National Research Foundation.
URL:https://ibecbarcelona.eu/event/ibec-seminar-dong-pyo-kim/
LOCATION:IBEC\, floor 11\, Tower I\, Baldiri Reixac 4-8\, 08028 Barcelona\, Spain
CATEGORIES:IBEC Seminar
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Europe/Madrid:20180118T150000
DTEND;TZID=Europe/Madrid:20180118T160000
DTSTAMP:20260405T213701
CREATED:20180115T090716Z
LAST-MODIFIED:20180115T090716Z
UID:96154-1516287600-1516291200@ibecbarcelona.eu
SUMMARY:IBEC Seminar: Gopi Shah
DESCRIPTION:Prospects of light sheet microscopy in developmental biology and cancer research\nGopi Shah (CRUK Cambridge Institute\, University of Cambridge)\nLight sheet microscopy is one of the fastest fluorescence imaging technologies available today. In the last decade\, it has emerged as an ideal technique for visualizing biological processes occurring at various time and length scales: rapid three-dimensional processes such as the beating zebrafish heart can be captured at >400 frames/sec\, large samples such as the developing zebrafish embryo (~0.7-1mm) can be imaged in toto at high resolution through multi-view imaging and delicate samples such as in vitro cultured 3D organoids can be monitored over days owing to its non-invasive nature. \nNonetheless\, most biological studies demand a higher imaging throughput in terms of sample size\, which has been a challenge for light sheet microscopy both in terms of microscope design and the volume of data generated. To address this\, we have developed customised light sheet microscopes with real-time image-processing engine that projects the 3D image volume onto a 2D map\, drastically reducing the amount of data generated as well as providing a panoramic view of the sample for ease of downstream analyses. We also designed a fluidic sample delivery system to pump embryos through the microscope\, enabling time-lapse imaging and screening of several samples simultaneously. Together\, these tools harness the high-speed imaging capability of a light sheet system to obtain multi-dimensional data from many samples\, essential for systematic population analysis. In my talk\, I will discuss how this work (a) has enabled integration of whole-sample live imaging\, genetic information and analysis of an ensemble of specimen to understand large-scale tissue movements during zebrafish embryogenesis and (b) facilitates my vision of establishing high-throughput imaging of organoids for understanding tumor cell dynamics and developing organoids as a model for image-based screening and therapeutics.
URL:https://ibecbarcelona.eu/event/ibec-seminar-gopi-shah-2/
LOCATION:IBEC\, floor 11\, Tower I\, Baldiri Reixac 4-8\, 08028 Barcelona\, Spain
CATEGORIES:IBEC Seminar
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Europe/Madrid:20180118T150000
DTEND;TZID=Europe/Madrid:20180118T160000
DTSTAMP:20260405T213701
CREATED:20180115T090716Z
LAST-MODIFIED:20180115T090716Z
UID:57187-1516287600-1516291200@ibecbarcelona.eu
SUMMARY:IBEC Seminar: Gopi Shah
DESCRIPTION:Prospects of light sheet microscopy in developmental biology and cancer research\nGopi Shah (CRUK Cambridge Institute\, University of Cambridge)\nLight sheet microscopy is one of the fastest fluorescence imaging technologies available today. In the last decade\, it has emerged as an ideal technique for visualizing biological processes occurring at various time and length scales: rapid three-dimensional processes such as the beating zebrafish heart can be captured at >400 frames/sec\, large samples such as the developing zebrafish embryo (~0.7-1mm) can be imaged in toto at high resolution through multi-view imaging and delicate samples such as in vitro cultured 3D organoids can be monitored over days owing to its non-invasive nature. \nNonetheless\, most biological studies demand a higher imaging throughput in terms of sample size\, which has been a challenge for light sheet microscopy both in terms of microscope design and the volume of data generated. To address this\, we have developed customised light sheet microscopes with real-time image-processing engine that projects the 3D image volume onto a 2D map\, drastically reducing the amount of data generated as well as providing a panoramic view of the sample for ease of downstream analyses. We also designed a fluidic sample delivery system to pump embryos through the microscope\, enabling time-lapse imaging and screening of several samples simultaneously. Together\, these tools harness the high-speed imaging capability of a light sheet system to obtain multi-dimensional data from many samples\, essential for systematic population analysis. In my talk\, I will discuss how this work (a) has enabled integration of whole-sample live imaging\, genetic information and analysis of an ensemble of specimen to understand large-scale tissue movements during zebrafish embryogenesis and (b) facilitates my vision of establishing high-throughput imaging of organoids for understanding tumor cell dynamics and developing organoids as a model for image-based screening and therapeutics.
URL:https://ibecbarcelona.eu/event/ibec-seminar-gopi-shah/
LOCATION:IBEC\, floor 11\, Tower I\, Baldiri Reixac 4-8\, 08028 Barcelona\, Spain
CATEGORIES:IBEC Seminar
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Europe/Madrid:20180131T153000
DTEND;TZID=Europe/Madrid:20180131T163000
DTSTAMP:20260405T213701
CREATED:20180125T121920Z
LAST-MODIFIED:20180129T101234Z
UID:57400-1517412600-1517416200@ibecbarcelona.eu
SUMMARY:IBEC Seminar: Gregory Lanza
DESCRIPTION:IBEC Seminar: Gregory Lanza\nGregory Lanza (Professor of Medicine\, Biomedical Engineering and Biology & Biomedical Sciences; Washington University)\nDr. Lanza is a full Professor of Medicine in the Cardiovascular Division of the School of Medicine\, with affiliations in the Department of Biomedical Engineering\, as well as Biology & Biomedical Sciences\, in Washington University.  He received a B.A. from Colby College\, both an M.S. and a Ph.D. from the Department of Poultry Science in University of Georgia Athens\, an M.D. from Northwest University\, and conducted his medical residency and cardiology specialization in Washington University Medical Center.  Dr. Lanza has been the recipient for a Searle Career Development Award\, as well as NCI Unconventional Innovation Program Awards in 2000\, 2002\, and 2003\, among several other recognitions.  He is also a Fellow of the American College of Cardiology.  Dr. Lanza is the Co-founder and Chief Scientific Officer for Kereos\, Inc.  He serves in the editorial board of Nanomedicine: Nanotechnology\, Biology\, & Medicine\, the International Journal of Green Nanotechnology: Biomedicine\, WIREs: Nanomedicine\, and Theranostics.
URL:https://ibecbarcelona.eu/event/ibec-seminar-gregory-lanza/
LOCATION:IBEC\, floor 11\, Tower I\, Baldiri Reixac 4-8\, 08028 Barcelona\, Spain
CATEGORIES:IBEC Seminar
ORGANIZER;CN="IBEC":MAILTO:www.ibecbarcelona.eu
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Europe/Madrid:20180131T153000
DTEND;TZID=Europe/Madrid:20180131T163000
DTSTAMP:20260405T213701
CREATED:20180125T121920Z
LAST-MODIFIED:20180125T121920Z
UID:96160-1517412600-1517416200@ibecbarcelona.eu
SUMMARY:IBEC Seminar: Gregory Lanza
DESCRIPTION:IBEC Seminar: Gregory Lanza\nGregory Lanza (Professor of Medicine\, Biomedical Engineering and Biology & Biomedical Sciences; Washington University)\nDr. Lanza is a full Professor of Medicine in the Cardiovascular Division of the School of Medicine\, with affiliations in the Department of Biomedical Engineering\, as well as Biology & Biomedical Sciences\, in Washington University.  He received a B.A. from Colby College\, both an M.S. and a Ph.D. from the Department of Poultry Science in University of Georgia Athens\, an M.D. from Northwest University\, and conducted his medical residency and cardiology specialization in Washington University Medical Center.  Dr. Lanza has been the recipient for a Searle Career Development Award\, as well as NCI Unconventional Innovation Program Awards in 2000\, 2002\, and 2003\, among several other recognitions.  He is also a Fellow of the American College of Cardiology.  Dr. Lanza is the Co-founder and Chief Scientific Officer for Kereos\, Inc.  He serves in the editorial board of Nanomedicine: Nanotechnology\, Biology\, & Medicine\, the International Journal of Green Nanotechnology: Biomedicine\, WIREs: Nanomedicine\, and Theranostics.
URL:https://ibecbarcelona.eu/event/ibec-seminar-gregory-lanza-2/
LOCATION:IBEC\, floor 11\, Tower I\, Baldiri Reixac 4-8\, 08028 Barcelona\, Spain
CATEGORIES:IBEC Seminar
ORGANIZER;CN="IBEC":MAILTO:www.ibecbarcelona.eu
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Europe/Madrid:20180228T100000
DTEND;TZID=Europe/Madrid:20180228T110000
DTSTAMP:20260405T213701
CREATED:20180201T164138Z
LAST-MODIFIED:20180201T164138Z
UID:96163-1519812000-1519815600@ibecbarcelona.eu
SUMMARY:IBEC Seminar: Johanna Ivaska
DESCRIPTION:Mechanosensitive regulation of cancer and pluripotency\nJohanna Ivaska\, Turku Centre for Biotechnology\, University of Turku\, Finland\nTissue homeostasis is dependent on the spatially controlled localization of specific cell types and the correct composition of the extracellular stroma. Integrin mediated adhesions\, in conjunction with the actin cytoskeleton\, allow cells to sense the stiffness of the surrounding extra-cellular matrix (ECM). Conversely\, cells exert acto-myosin and integrin dependent forces to remodel and organize the surrounding ECM. In cancer\, stiffening of the tumor stroma is considered as an instrumental contributor to tumor progression. However\, the mechanisms how stromal ECM regulates cancer progression is not fully understood. I will describe our recent findings on the interrelationship between cancer cell mediated ECM remodelling and ECM induced mechanochemical signals regulating transcription of growth promoting pathways in cancer cells. Reprogramming and survival of human pluripotent stem cells is heavily influenced by their adhesion to the underlying ECM. We have recently investigated the link between ECM-adhesion\, the actin cytoskeleton and cell contractility in maintenance of pluripotency. I will describe our recent efforts to define the stem-cell adhesion structure in nanoscale and how it contributes to maintenance of pluripotency.
URL:https://ibecbarcelona.eu/event/ibec-seminar-johanna-ivaska-2/
LOCATION:IBEC\, floor 11\, Tower I\, Baldiri Reixac 4-8\, 08028 Barcelona\, Spain
CATEGORIES:IBEC Seminar
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Europe/Madrid:20180228T100000
DTEND;TZID=Europe/Madrid:20180228T110000
DTSTAMP:20260405T213701
CREATED:20180201T164138Z
LAST-MODIFIED:20180201T164148Z
UID:57497-1519812000-1519815600@ibecbarcelona.eu
SUMMARY:IBEC Seminar: Johanna Ivaska
DESCRIPTION:Mechanosensitive regulation of cancer and pluripotency\nJohanna Ivaska\, Turku Centre for Biotechnology\, University of Turku\, Finland\nTissue homeostasis is dependent on the spatially controlled localization of specific cell types and the correct composition of the extracellular stroma. Integrin mediated adhesions\, in conjunction with the actin cytoskeleton\, allow cells to sense the stiffness of the surrounding extra-cellular matrix (ECM). Conversely\, cells exert acto-myosin and integrin dependent forces to remodel and organize the surrounding ECM. In cancer\, stiffening of the tumor stroma is considered as an instrumental contributor to tumor progression. However\, the mechanisms how stromal ECM regulates cancer progression is not fully understood. I will describe our recent findings on the interrelationship between cancer cell mediated ECM remodelling and ECM induced mechanochemical signals regulating transcription of growth promoting pathways in cancer cells. Reprogramming and survival of human pluripotent stem cells is heavily influenced by their adhesion to the underlying ECM. We have recently investigated the link between ECM-adhesion\, the actin cytoskeleton and cell contractility in maintenance of pluripotency. I will describe our recent efforts to define the stem-cell adhesion structure in nanoscale and how it contributes to maintenance of pluripotency.
URL:https://ibecbarcelona.eu/event/ibec-seminar-johanna-ivaska/
LOCATION:IBEC\, floor 11\, Tower I\, Baldiri Reixac 4-8\, 08028 Barcelona\, Spain
CATEGORIES:IBEC Seminar
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Europe/Madrid:20180320T160000
DTEND;TZID=Europe/Madrid:20180320T170000
DTSTAMP:20260405T213701
CREATED:20180312T101441Z
LAST-MODIFIED:20180312T101441Z
UID:96186-1521561600-1521565200@ibecbarcelona.eu
SUMMARY:IBEC Seminar: Carlo A. Bortolotti
DESCRIPTION:Monitoring biorecognition with organic bioelectronic transistors\nCarlo A. Bortolotti\, Dipartimento di Scienze della Vita\, Universita di Modena ed Regio Emilia\, Italy\nElectrolyte-gated OFETs (EGOFETs) and Organic Electrochemical transistors (OECTs) are emerging as an important class of chemo- and biosensors to meet the main requirements of healthcare diagnostics: portability\, manufacturing with low cost\, miniaturization\, low-temperature processing. These devices can be operated either in accumulation (EGOFETs) or in depletion mode (OECTs). Devices that allow transduction of protein/protein interactions can be used not only for analytical purposes\, but also for real time monitoring of surface adsorption and recognition events\, and may therefore provide insights into both the kinetics and thermodynamics of biomolecular interactions. These devices provide a real-time\, label-free response and the ultra-low sensitivity arising from the capacitive coupling between the electrolyte solution and the channel. We are currently investigating a wide range of biorecognition events\, differing in terms of size of the surface bound biomolecule and of the chemical nature and lateral dimensions of the biological partner in solution\, ranging from receptor/ligand interactions to antibody/antigene (protein) and antibody/virus couples. I will present a few examples of the EGOFET-based and OECT-based detection of detection of biorecognition events. Different surface functionalization strategies\, aiming at reducing non-specific binding\, increasing sensitivity and ensuring re-usability of the electrodes with immobilized sensing units will be described. I will also present our latest achievements in the development of a multigate lab-on-a-chip device\, aiming at the multiplexed detection of different analytes in a biological fluid\, also including an internal reference electrode.
URL:https://ibecbarcelona.eu/event/ibec-seminar-carlo-a-bortolotti-2/
LOCATION:IBEC\, floor 11\, Tower I\, Baldiri Reixac 4-8\, 08028 Barcelona\, Spain
CATEGORIES:IBEC Seminar
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Europe/Madrid:20180320T160000
DTEND;TZID=Europe/Madrid:20180320T170000
DTSTAMP:20260405T213701
CREATED:20180312T101441Z
LAST-MODIFIED:20180312T101441Z
UID:57987-1521561600-1521565200@ibecbarcelona.eu
SUMMARY:IBEC Seminar: Carlo A. Bortolotti
DESCRIPTION:Monitoring biorecognition with organic bioelectronic transistors\nCarlo A. Bortolotti\, Dipartimento di Scienze della Vita\, Universita di Modena ed Regio Emilia\, Italy\nElectrolyte-gated OFETs (EGOFETs) and Organic Electrochemical transistors (OECTs) are emerging as an important class of chemo- and biosensors to meet the main requirements of healthcare diagnostics: portability\, manufacturing with low cost\, miniaturization\, low-temperature processing. These devices can be operated either in accumulation (EGOFETs) or in depletion mode (OECTs). Devices that allow transduction of protein/protein interactions can be used not only for analytical purposes\, but also for real time monitoring of surface adsorption and recognition events\, and may therefore provide insights into both the kinetics and thermodynamics of biomolecular interactions. These devices provide a real-time\, label-free response and the ultra-low sensitivity arising from the capacitive coupling between the electrolyte solution and the channel. We are currently investigating a wide range of biorecognition events\, differing in terms of size of the surface bound biomolecule and of the chemical nature and lateral dimensions of the biological partner in solution\, ranging from receptor/ligand interactions to antibody/antigene (protein) and antibody/virus couples. I will present a few examples of the EGOFET-based and OECT-based detection of detection of biorecognition events. Different surface functionalization strategies\, aiming at reducing non-specific binding\, increasing sensitivity and ensuring re-usability of the electrodes with immobilized sensing units will be described. I will also present our latest achievements in the development of a multigate lab-on-a-chip device\, aiming at the multiplexed detection of different analytes in a biological fluid\, also including an internal reference electrode.
URL:https://ibecbarcelona.eu/event/ibec-seminar-carlo-a-bortolotti/
LOCATION:IBEC\, floor 11\, Tower I\, Baldiri Reixac 4-8\, 08028 Barcelona\, Spain
CATEGORIES:IBEC Seminar
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Europe/Madrid:20180328T100000
DTEND;TZID=Europe/Madrid:20180328T110000
DTSTAMP:20260405T213701
CREATED:20180322T115517Z
LAST-MODIFIED:20180322T115517Z
UID:96199-1522231200-1522234800@ibecbarcelona.eu
SUMMARY:IBEC Seminar: Aurélien Bancaud
DESCRIPTION:µLAS technology for DNA processing: setting up elementary functions (concentration\, separation\, purification\, identification) and application in oncology and targeted sequencing\nAurélien Bancaud\, LAAS-CNRS\, Toulouse\, France\nWe recently developed the µLAS technology for nucleic acids processing. Its operating principle relies on the monitoring of DNA transport in a viscoelastic liquid under the combined action of hydrodynamic and electrophoretic forces (1). DNA molecules are dragged toward the walls of microchannels by a transverse force proportional to their contour length. Because the hydrodynamic decreases near the wall\, DNA molecules are sorted according to their molecular weight. Furthermore\, by tailoring the geometry of a channel with a constriction\, we can tune the amplitude of transverse forces and stop molecules to design a concentrator that achieves enrichment rates of 100 to 1000 fold per minute. \nWe will derive a quantitative model of DNA transport in µLAS that relies on 1 fitting parameter and perform rational optimizations of the technology. We will then exploit µLAS for sizing cell-free circulating DNA (cfDNA) in the blood (2)\, and demonstrate that cfDNA profiling is a promissing biomarker for the follow-up of cancer patients. Finally\, we will present the principle of a DNA size-selective valve for the purification and sequencing of target genomic regions by combining µLAS with Cas9 endonuclease. \n(1) Ranchon et al.\, Lab Chip (2016)\n (2) Andriamanampisoa et al.\, Anal Chem (2018)
URL:https://ibecbarcelona.eu/event/ibec-seminar-aurelien-bancaud-2/
LOCATION:IBEC\, floor 11\, Tower I\, Baldiri Reixac 4-8\, 08028 Barcelona\, Spain
CATEGORIES:IBEC Seminar
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Europe/Madrid:20180328T100000
DTEND;TZID=Europe/Madrid:20180328T110000
DTSTAMP:20260405T213701
CREATED:20180322T115517Z
LAST-MODIFIED:20180322T115517Z
UID:58142-1522231200-1522234800@ibecbarcelona.eu
SUMMARY:IBEC Seminar: Aurélien Bancaud
DESCRIPTION:µLAS technology for DNA processing: setting up elementary functions (concentration\, separation\, purification\, identification) and application in oncology and targeted sequencing\nAurélien Bancaud\, LAAS-CNRS\, Toulouse\, France\nWe recently developed the µLAS technology for nucleic acids processing. Its operating principle relies on the monitoring of DNA transport in a viscoelastic liquid under the combined action of hydrodynamic and electrophoretic forces (1). DNA molecules are dragged toward the walls of microchannels by a transverse force proportional to their contour length. Because the hydrodynamic decreases near the wall\, DNA molecules are sorted according to their molecular weight. Furthermore\, by tailoring the geometry of a channel with a constriction\, we can tune the amplitude of transverse forces and stop molecules to design a concentrator that achieves enrichment rates of 100 to 1000 fold per minute. \nWe will derive a quantitative model of DNA transport in µLAS that relies on 1 fitting parameter and perform rational optimizations of the technology. We will then exploit µLAS for sizing cell-free circulating DNA (cfDNA) in the blood (2)\, and demonstrate that cfDNA profiling is a promissing biomarker for the follow-up of cancer patients. Finally\, we will present the principle of a DNA size-selective valve for the purification and sequencing of target genomic regions by combining µLAS with Cas9 endonuclease. \n(1) Ranchon et al.\, Lab Chip (2016)\n (2) Andriamanampisoa et al.\, Anal Chem (2018)
URL:https://ibecbarcelona.eu/event/ibec-seminar-aurelien-bancaud/
LOCATION:IBEC\, floor 11\, Tower I\, Baldiri Reixac 4-8\, 08028 Barcelona\, Spain
CATEGORIES:IBEC Seminar
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Europe/Madrid:20180404T120000
DTEND;TZID=Europe/Madrid:20180404T130000
DTSTAMP:20260405T213701
CREATED:20180222T110811Z
LAST-MODIFIED:20180222T110811Z
UID:96178-1522843200-1522846800@ibecbarcelona.eu
SUMMARY:IBEC Seminar: Fernando López-Gallego
DESCRIPTION:Immobilization of multi-enzyme systems; an avenue to fabricate self-sufficient heterogeneous biocatalysts\nFernando López-Gallego\, Heterogeneous Biocatalysis Lab\, University of Zaragoza (iQSCH-CSIC) / ARAID\, Science Foundation of Aragón\nIn the last decade\, the chemists have been delighted by the catalytic orchestration found in vivo\, and have isolated multi-enzyme system to work ex-vivo in both natural and non-natural tandem reactions creating a new concept: systems biocatalysis. These systems are the pioneers of the cell-free synthetic biology; an emerging discipline that seeks the simplest biology to make the most complex chemistry. We have paid our attention to the heterogenization of multi-enzyme system to catalyze tandem reactions. Co-immobilization of multi-enzyme systems improve: 1) the kinetics of the chemical cascades due to the spatial localization of the different biocatalytic modules that avoids intermediate accumulation and increases cofactor recycling efficiency\, 2) the stability of the biocatalysts due to both structural rigidification and in situ elimination of toxic by-products\, 3) the biocatalyst recycle and 4) the biocatalyst adaptation to continuous processes. Nevertheless\, the co-immobilization of several enzymes to carry out synthetic cascades is challenging because there is no a universal immobilization chemistry that optimally attaches all the enzymes to the same surface. We have recently developed different immobilized multi-enzyme systems formed by a 3-enzyme cascade for oxidizing phenol derivatives with in situ H2O2 supply\, a 3-enzyme cascade for synthesizing 1\,3-dihydroxyacetone with both in situ cofactor recycling and H2O2 elimination\, a 4-enzyme cascade for quantitatively synthesizing pro-chiral ketones starting from racemic esters and a 2-enzyme cascades to synthesize optically pure secondary alcohols integrating the cofactor recycling in the solid-phase. The optimal design of the immobilization protocols enables co-immobilizing several enzymes and cofactors on the porous carrier to optimize their spatial localization across the carrier microstructure and preserve both global activity and stability of the multi-enzyme systems.
URL:https://ibecbarcelona.eu/event/ibec-seminar-fernando-lopez-gallego-2/
LOCATION:IBEC\, floor 11\, Tower I\, Baldiri Reixac 4-8\, 08028 Barcelona\, Spain
CATEGORIES:IBEC Seminar
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Europe/Madrid:20180404T120000
DTEND;TZID=Europe/Madrid:20180404T130000
DTSTAMP:20260405T213701
CREATED:20180222T110811Z
LAST-MODIFIED:20180222T110811Z
UID:57824-1522843200-1522846800@ibecbarcelona.eu
SUMMARY:IBEC Seminar: Fernando López-Gallego
DESCRIPTION:Immobilization of multi-enzyme systems; an avenue to fabricate self-sufficient heterogeneous biocatalysts\nFernando López-Gallego\, Heterogeneous Biocatalysis Lab\, University of Zaragoza (iQSCH-CSIC) / ARAID\, Science Foundation of Aragón\nIn the last decade\, the chemists have been delighted by the catalytic orchestration found in vivo\, and have isolated multi-enzyme system to work ex-vivo in both natural and non-natural tandem reactions creating a new concept: systems biocatalysis. These systems are the pioneers of the cell-free synthetic biology; an emerging discipline that seeks the simplest biology to make the most complex chemistry. We have paid our attention to the heterogenization of multi-enzyme system to catalyze tandem reactions. Co-immobilization of multi-enzyme systems improve: 1) the kinetics of the chemical cascades due to the spatial localization of the different biocatalytic modules that avoids intermediate accumulation and increases cofactor recycling efficiency\, 2) the stability of the biocatalysts due to both structural rigidification and in situ elimination of toxic by-products\, 3) the biocatalyst recycle and 4) the biocatalyst adaptation to continuous processes. Nevertheless\, the co-immobilization of several enzymes to carry out synthetic cascades is challenging because there is no a universal immobilization chemistry that optimally attaches all the enzymes to the same surface. We have recently developed different immobilized multi-enzyme systems formed by a 3-enzyme cascade for oxidizing phenol derivatives with in situ H2O2 supply\, a 3-enzyme cascade for synthesizing 1\,3-dihydroxyacetone with both in situ cofactor recycling and H2O2 elimination\, a 4-enzyme cascade for quantitatively synthesizing pro-chiral ketones starting from racemic esters and a 2-enzyme cascades to synthesize optically pure secondary alcohols integrating the cofactor recycling in the solid-phase. The optimal design of the immobilization protocols enables co-immobilizing several enzymes and cofactors on the porous carrier to optimize their spatial localization across the carrier microstructure and preserve both global activity and stability of the multi-enzyme systems.
URL:https://ibecbarcelona.eu/event/ibec-seminar-fernando-lopez-gallego/
LOCATION:IBEC\, floor 11\, Tower I\, Baldiri Reixac 4-8\, 08028 Barcelona\, Spain
CATEGORIES:IBEC Seminar
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Europe/Madrid:20180413T100000
DTEND;TZID=Europe/Madrid:20180413T110000
DTSTAMP:20260405T213701
CREATED:20180226T153414Z
LAST-MODIFIED:20180226T153414Z
UID:96181-1523613600-1523617200@ibecbarcelona.eu
SUMMARY:IBEC Seminar: Joan Seoane
DESCRIPTION:Intratumor heterogeneity in brain cancer\nJoan Seoane\, Group Leader / Director of Translational Research at Vall d’Hebron Institute of Oncology (VHIO)\nJoan Seoane is a Group Leader and Director of the Translational Research program at the Vall d’Hebron Institute of Oncology (VHIO) within the Vall d’Hebron University Hospital since 2011. \nIn 1998\, Joan obtained his PhD in Biochemistry and Molecular Biology from the University of Barcelona. Previously\, in 1993\, he obtained his BSc degree in Chemistry. Joan joined the Memorial Sloan-Kettering Cancer Center (MSKCC) in New York as a post-doctoral fellow in 1998. \nFrom 1998 to 2001\, he worked as a Research Fellow and subsequently\, from 2001 to 2003\, as a Research Associate. He was appointed ICREA Research Professor in 2004 and joined VHIO. \nIn 2007\, he became a member of the EMBO Young Investigator program and the recipient of a European Research Council (ERC) grant in 2008. Later\, he obtained two ERC Proof of Concept grants (2011\, 2013). In 2008\, he became Board member of the European Association of Cancer Research (EACR) and Professor of the Autonomous University of Barcelona. \nIn 2012\, founded Mosaic Biomedicals as a spin-off company from his lab and\, in 2013\, he was the recipient of the Dr. Josef Steiner Award. In 2016\, he became Secretary General of the EACR.
URL:https://ibecbarcelona.eu/event/ibec-seminar-joan-seoane-2/
LOCATION:IBEC\, floor 11\, Tower I\, Baldiri Reixac 4-8\, 08028 Barcelona\, Spain
CATEGORIES:IBEC Seminar
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Europe/Madrid:20180413T100000
DTEND;TZID=Europe/Madrid:20180413T110000
DTSTAMP:20260405T213701
CREATED:20180226T153414Z
LAST-MODIFIED:20180409T075629Z
UID:57830-1523613600-1523617200@ibecbarcelona.eu
SUMMARY:IBEC Seminar: Joan Seoane
DESCRIPTION:Intratumor heterogeneity in brain cancer\nJoan Seoane\, Group Leader / Director of Translational Research at Vall d’Hebron Institute of Oncology (VHIO)\nJoan Seoane is a Group Leader and Director of the Translational Research program at the Vall d’Hebron Institute of Oncology (VHIO) within the Vall d’Hebron University Hospital since 2011. \nIn 1998\, Joan obtained his PhD in Biochemistry and Molecular Biology from the University of Barcelona. Previously\, in 1993\, he obtained his BSc degree in Chemistry. Joan joined the Memorial Sloan-Kettering Cancer Center (MSKCC) in New York as a post-doctoral fellow in 1998. \nFrom 1998 to 2001\, he worked as a Research Fellow and subsequently\, from 2001 to 2003\, as a Research Associate. He was appointed ICREA Research Professor in 2004 and joined VHIO. \nIn 2007\, he became a member of the EMBO Young Investigator program and the recipient of a European Research Council (ERC) grant in 2008. Later\, he obtained two ERC Proof of Concept grants (2011\, 2013). In 2008\, he became Board member of the European Association of Cancer Research (EACR) and Professor of the Autonomous University of Barcelona. \nIn 2012\, founded Mosaic Biomedicals as a spin-off company from his lab and\, in 2013\, he was the recipient of the Dr. Josef Steiner Award. In 2016\, he became Secretary General of the EACR.
URL:https://ibecbarcelona.eu/event/ibec-seminar-joan-seoane/
LOCATION:IBEC\, floor 11\, Tower I\, Baldiri Reixac 4-8\, 08028 Barcelona\, Spain
CATEGORIES:IBEC Seminar
END:VEVENT
END:VCALENDAR