BEGIN:VCALENDAR
VERSION:2.0
PRODID:-//Institute for Bioengineering of Catalonia - ECPv6.15.20//NONSGML v1.0//EN
CALSCALE:GREGORIAN
METHOD:PUBLISH
X-ORIGINAL-URL:https://ibecbarcelona.eu
X-WR-CALDESC:Events for Institute for Bioengineering of Catalonia
REFRESH-INTERVAL;VALUE=DURATION:PT1H
X-Robots-Tag:noindex
X-PUBLISHED-TTL:PT1H
BEGIN:VTIMEZONE
TZID:Europe/Madrid
BEGIN:DAYLIGHT
TZOFFSETFROM:+0100
TZOFFSETTO:+0200
TZNAME:CEST
DTSTART:20200329T010000
END:DAYLIGHT
BEGIN:STANDARD
TZOFFSETFROM:+0200
TZOFFSETTO:+0100
TZNAME:CET
DTSTART:20201025T010000
END:STANDARD
BEGIN:DAYLIGHT
TZOFFSETFROM:+0100
TZOFFSETTO:+0200
TZNAME:CEST
DTSTART:20210328T010000
END:DAYLIGHT
BEGIN:STANDARD
TZOFFSETFROM:+0200
TZOFFSETTO:+0100
TZNAME:CET
DTSTART:20211031T010000
END:STANDARD
BEGIN:DAYLIGHT
TZOFFSETFROM:+0100
TZOFFSETTO:+0200
TZNAME:CEST
DTSTART:20220327T010000
END:DAYLIGHT
BEGIN:STANDARD
TZOFFSETFROM:+0200
TZOFFSETTO:+0100
TZNAME:CET
DTSTART:20221030T010000
END:STANDARD
END:VTIMEZONE
BEGIN:VEVENT
DTSTART;TZID=Europe/Madrid:20211126T100000
DTEND;TZID=Europe/Madrid:20211126T120000
DTSTAMP:20260425T010238
CREATED:20210909T094150Z
LAST-MODIFIED:20211117T150528Z
UID:87087-1637920800-1637928000@ibecbarcelona.eu
SUMMARY:PhD Discussions: Yolanda Castillo and Marc Molina
DESCRIPTION:Assessment of trunk function in patients with spinal cord injury using electromyography and smartphone accelerometry\nYolanda Castillo\, Biomedical Signal Processing and Interpretation \nSpinal cord injury (SCI) causes motor and sensory impairment below the level of the injury\, but also many other health problems. A common consequence of SCI is the lack of control over trunk muscles\, leading to deficits in postural control and balance while sitting. Since trunk stability is essential to maintain upright posture and support functional movements\, impaired trunk function constitutes a major cause of motor disability in SCI patients\, limiting their independence and quality of life. However\, trunk stability is rarely examined in studies of mobility after SCI\, and one of the reasons is the lack of quantitative measures for assessing trunk function. Here we propose to record and analyze electromyographic (EMG) and smartphone accelerometric data to extract quantitative measures for the evaluation of trunk function. Our aims were: 1) to characterize muscle activity and movement patterns of trunk flexion during a reaching task in healthy subjects and patients with SCI\, 2) to compare the impact of cervical and thoracic injuries in trunk function\, and 3) to investigate the potentially destabilizing effects of a startling acoustic stimulus in this task. For these purposes\, during a reaching movement requiring trunk flexion\, we recorded the EMG activity of 8 trunk\, neck\, and shoulder muscles and smartphone accelerometer data from individuals with cervical SCI\, thoracic SCI\, and healthy control subjects. We analyzed these signals and extracted different features\, including the response time until pressing a target button\, EMG onset latencies and amplitudes\, and trunk tilt\, lateral deviation\, and other movement features from accelerometry. The proposed outcome measures revealed deficits in postural control and compensatory strategies employed by SCI patients\, including delayed responses and higher lateral deviations\, which might have important consequences for rehabilitation. The combination of EMG and smartphone accelerometer data can help to develop more suitable methods for the assessment of trunk function in individuals with SCI\, thus improving the follow-up and management of these patients. \nDevelopment of a model of “macro” substrates for the analysis of 3D chromatin structure and transcriptional profiling\nMarc Molina\, Cellular and Molecular Mechanobiology \nMechanically-induced changes in the genome are increasingly recognized as major drivers of cell and tissue function. However\, current and past studies on this topic in vitro have often been limited by the sample size required for these genomic analyses. Here we describe the development of a polyacrylamide gel (pAAg) substrate with larger area than gels previously generated in labs worldwide. These substrates display the same tunable stiffness as their smaller counterparts and are particularly suitable to accommodate large numbers of cells. We tested the substrates to assess the effect that changes in rigidity have in the cell’s genome\, both at the level of chromatin organization and transcriptional regulation. For this\, two different rigidities were used (soft vs stiff) with three conditions which included: i) a control group\, ii) a transiently expressing mutant of RanQ69L that prevents all nucleocytoplasmic transport and iii) a transiently expressing mutant of NES1-KASH that prevents force transmission to the nucleus. Our preliminary results suggest that cells seeded on pAAg substrates of different rigidities display differences in chromatin structure and gene expression\, but more data will be necessary to further support these results. Ultimately\, the aim of our project is to understand down the road how changes in rigidity affect: i) the 3D structure and interaction map of chromatin and ii) the activated or repressed transcriptional programs in soft and stiff substrates. Overall\, this new model will help us to characterize how the genome is affected by rigidity both at the structural and transcriptional levels. More broadly\, we expect the potential findings of this work to help the community detailing the effect of changes in tissue stiffness in the genome spatial organization. \nThis PhD Discussion will be hybrid. Yolanda Castillo will be doing her presentation online and Marc from the Baobab room\, located in tower I floor 11. To follow the session online\, find here the link\, we will be using the GoToMeeting app. If you wish to attend in person\, the free spots will be assigned on a first come first served basis\, the capacity of the room is for 30 people.
URL:https://ibecbarcelona.eu/event/phd-discussions-yolanda-castillo-and-marc-molina/
CATEGORIES:PhD Discussions Session
END:VEVENT
END:VCALENDAR