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X-WR-CALNAME:Institute for Bioengineering of Catalonia
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X-WR-CALDESC:Events for Institute for Bioengineering of Catalonia
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DTSTART;TZID=Europe/Madrid:20150710T100000
DTEND;TZID=Europe/Madrid:20150710T110000
DTSTAMP:20260517T010650
CREATED:20150527T073448Z
LAST-MODIFIED:20150527T073448Z
UID:95855-1436522400-1436526000@ibecbarcelona.eu
SUMMARY:PhD Discussions Session: Verónica Hortigüela & Anna Crespo
DESCRIPTION:Developing a platform for receptor clustering studies\nVerónica Hortigüela\, Biomimetic systems for cell engineering group\nReceptors are signaling units that usually require interactions and associations with other molecules in complexes to trigger a signaling pathway. This process is known as receptor clustering and comes typically along with a simultaneous ligand clustering underneath the cell membrane. We have developed a strategy to precisely control the ligand distribution on a substrate at the nanoscale to study in detail receptor clustering processes. Herein we present a tunable platform based on self-assembled di-block copolymers that tend to segregate into nanostructures. Di-block copolymers are confined to a thin film providing a template for ligand patterning.  \n  \nRibonucleotide Reductase anaerobic enzymes are essential for biofilm formation of Pseudomonas aeruginosa\nAnna Crespo\, Bacterial infections: antimicrobial therapies group\nMost chronic infections in humans are caused by communities of microorganisms living in organized structures\, known as biofilms. Biofilm-related infections\, such as pneumonia (in patients suffering for cystic fibrosis or chronic obstructive pulmonary disease –COPD-) and catheter-associated infections\, affect millions of people in the developed world. \nCell clusters in biofilms are characterized by presenting\, in its extracellular polymeric matrix\, gradients of oxygen\, nutrients and metabolic waste products. The so-formed chemical heterogeneity (e.g.\, the presence of anoxic areas) leads to the appearance of different metabolic activities. \nPseudomonas aeruginosa has been used as a model bacterium for biofilm research; it causes biofilm-related chronic infections and presents high metabolic versatility\, together with an extreme antibiotic resistance. \nIn this work we have studied P. aeruginosa\, focusing in an essential enzyme for its growth\, Ribonucleotide Reductase (RNR). Ribonucleotide Reductases catalyse the reduction of ribonucleotides (NTPs) to deoxyribonucleotides (dNTPs)\, thereby providing the building blocks for DNA synthesis. There are three different RNR classes\, named class I\, class II and class III\, which are\, respectively\, oxygen-dependent\, oxygen-independent and oxygen-sensitive. The last two ones\, essential for anaerobic growth in Pseudomonas aeruginosa\, have been proved to be necessary for biofilm formation\, and therefore putative targets for new therapies against P. aeruginosa chronic infections.
URL:https://ibecbarcelona.eu/event/phd-discussions-session-veronica-hortiguela-anna-crespo-2/
CATEGORIES:PhD Discussions Session
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Europe/Madrid:20150529T100000
DTEND;TZID=Europe/Madrid:20150529T110000
DTSTAMP:20260517T010650
CREATED:20150527T072015Z
LAST-MODIFIED:20150527T072015Z
UID:16865-1432893600-1432897200@ibecbarcelona.eu
SUMMARY:PhD Discussions Sessions: Lorena de Oñate & Anita Kosmalska
DESCRIPTION:Research on cardiac differentiation from pluripotent stem cells: how to get beating cells in a dish\nLorena de Oñate\, Pluripotent stem cells and activation of endogenous tissue programs for organ regeneration group\nProbably\, the gain in organ complexity and cell function has led to a decrease in healing capacities in the adult mammalian heart. In an effort to generate new venues for the generation of functional cardiac cells we have explored the possibility to manipulate cell fate and plasticity making use of different cellular systems. First\, taking advantage of pluripotent stem cells we have defined chemically based protocols in order to generate cardiac cells from human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSC). Second\, by a cell conversion approach\, we have been able to produce cardiomyocyte-like cells from human fibroblasts by overexpression of specific lineage transcription factors. In parallel\, to overcome several drawbacks related to both processes (i.e: purity of final cell populations)\, we have efficiently developed a reporter cell line for the cardiac gene alpha Myosin Heavy Chain (MYH6) by both TALEN and CRISPR/CAS9 genome editing approaches that will help us to define accurate protocols for cardiac differentiation\, and more importantly\, to underscore the molecular and cellular events driving human cardiomyocyte differentiation. \n  \nPhysical principles of membrane remodelling during cell mechanoadaptation\nAnita Kosmalska\, Cellular and respiratory biomechanic group\nBiological processes in any physiological environment involve changes in cell shape\, which must be accommodated by their physical envelope – the bilayer membrane. However\, the fundamental biophysical principles by which the cell membrane allows for and responds to shape changes remain unclear. Here we show that the 3D remodelling of the membrane in response to a broad diversity of physiological perturbations can be explained by a purely mechanical process. This process is passive\, local\, almost instantaneous\, prior to any active remodelling\, and generates different types of membrane invaginations that can repeatedly store and release large fractions of the cell membrane. We further demonstrate that the shape of those invaginations is determined by the minimum elastic and adhesive energy required to store both membrane area and liquid volume at the cell-substrate interface. Once formed\, cells reabsorb the invaginations through an active process with duration of the order of minutes.
URL:https://ibecbarcelona.eu/event/phd-discussions-sessions-lorena-de-onate-anita-kosmalska/
CATEGORIES:PhD Discussions Session
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Europe/Madrid:20150529T100000
DTEND;TZID=Europe/Madrid:20150529T110000
DTSTAMP:20260517T010650
CREATED:20150527T072015Z
LAST-MODIFIED:20150527T072015Z
UID:95853-1432893600-1432897200@ibecbarcelona.eu
SUMMARY:PhD Discussions Sessions: Lorena de Oñate & Anita Kosmalska
DESCRIPTION:Research on cardiac differentiation from pluripotent stem cells: how to get beating cells in a dish\nLorena de Oñate\, Pluripotent stem cells and activation of endogenous tissue programs for organ regeneration group\nProbably\, the gain in organ complexity and cell function has led to a decrease in healing capacities in the adult mammalian heart. In an effort to generate new venues for the generation of functional cardiac cells we have explored the possibility to manipulate cell fate and plasticity making use of different cellular systems. First\, taking advantage of pluripotent stem cells we have defined chemically based protocols in order to generate cardiac cells from human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSC). Second\, by a cell conversion approach\, we have been able to produce cardiomyocyte-like cells from human fibroblasts by overexpression of specific lineage transcription factors. In parallel\, to overcome several drawbacks related to both processes (i.e: purity of final cell populations)\, we have efficiently developed a reporter cell line for the cardiac gene alpha Myosin Heavy Chain (MYH6) by both TALEN and CRISPR/CAS9 genome editing approaches that will help us to define accurate protocols for cardiac differentiation\, and more importantly\, to underscore the molecular and cellular events driving human cardiomyocyte differentiation. \n  \nPhysical principles of membrane remodelling during cell mechanoadaptation\nAnita Kosmalska\, Cellular and respiratory biomechanic group\nBiological processes in any physiological environment involve changes in cell shape\, which must be accommodated by their physical envelope – the bilayer membrane. However\, the fundamental biophysical principles by which the cell membrane allows for and responds to shape changes remain unclear. Here we show that the 3D remodelling of the membrane in response to a broad diversity of physiological perturbations can be explained by a purely mechanical process. This process is passive\, local\, almost instantaneous\, prior to any active remodelling\, and generates different types of membrane invaginations that can repeatedly store and release large fractions of the cell membrane. We further demonstrate that the shape of those invaginations is determined by the minimum elastic and adhesive energy required to store both membrane area and liquid volume at the cell-substrate interface. Once formed\, cells reabsorb the invaginations through an active process with duration of the order of minutes.
URL:https://ibecbarcelona.eu/event/phd-discussions-sessions-lorena-de-onate-anita-kosmalska-2/
CATEGORIES:PhD Discussions Session
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Europe/Madrid:20150327T100000
DTEND;TZID=Europe/Madrid:20150327T110000
DTSTAMP:20260517T010650
CREATED:20150119T113556Z
LAST-MODIFIED:20150319T143821Z
UID:11717-1427450400-1427454000@ibecbarcelona.eu
SUMMARY:PhD Discussion Sessions: Claudia di Guglielmo and Oiane Urra
DESCRIPTION:Biotechnological approaches to cardiac differentiation of human induced pluripotent stem cells\nClaudia di Guglielmo\, Control of stem cell potency group\nHeart diseases are a major cause of death and disability in developed countries. Human induced pluripotent stem cells can be differentiated into cardiomyocytes\, representing a valid tool to be used as a platform for research and clinical applications in regenerative medicine. However\, current differentiation protocols have disadvantages related to insufficient purity and lack of scalability. We applied biotechnological strategies such as the use of transgenic cell lines and tissue engineering devices to overcome those drawbacks. This approach offers the possibility to dissect the mechanisms underlying cardiac differentiation\, as well as providing valuable in vitro systems for drug screening of patient-specific heart muscle cells. \n  \nMuscle Synergy analysis as a tool to improve stroke rehabilitation\nOiane Urra\, Biomedical signal processing and interpretation group\nStroke is the first cause of adult disability with upper-limb hemiparesis being the most frequent dysfunction. After rehabilitation\, one third of the patients result permanently disabled. Consequently\, intensive research aiming to develop novel therapies promoting neuroplasticity are being carried out to allow rehabilitation of these patients. In this thesis\, we propose to combine novel neurorehabilitation and biofeedback strategies and to evaluate its therapeutic effect from a physiological (changes at muscular and neuroplastic level)\, kinematic and functional perspective with the objective of guiding the design of more successful and cost-effective therapies.
URL:https://ibecbarcelona.eu/event/phd-discussion-sessions-claudia-di-guglielmo-and-oiane-urra/
CATEGORIES:PhD Discussions Session
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Europe/Madrid:20150327T100000
DTEND;TZID=Europe/Madrid:20150327T110000
DTSTAMP:20260517T010650
CREATED:20150119T113556Z
LAST-MODIFIED:20150119T113556Z
UID:95836-1427450400-1427454000@ibecbarcelona.eu
SUMMARY:PhD Discussion Sessions: Claudia di Guglielmo and Oiane Urra
DESCRIPTION:Biotechnological approaches to cardiac differentiation of human induced pluripotent stem cells\nClaudia di Guglielmo\, Control of stem cell potency group\nHeart diseases are a major cause of death and disability in developed countries. Human induced pluripotent stem cells can be differentiated into cardiomyocytes\, representing a valid tool to be used as a platform for research and clinical applications in regenerative medicine. However\, current differentiation protocols have disadvantages related to insufficient purity and lack of scalability. We applied biotechnological strategies such as the use of transgenic cell lines and tissue engineering devices to overcome those drawbacks. This approach offers the possibility to dissect the mechanisms underlying cardiac differentiation\, as well as providing valuable in vitro systems for drug screening of patient-specific heart muscle cells. \n  \nMuscle Synergy analysis as a tool to improve stroke rehabilitation\nOiane Urra\, Biomedical signal processing and interpretation group\nStroke is the first cause of adult disability with upper-limb hemiparesis being the most frequent dysfunction. After rehabilitation\, one third of the patients result permanently disabled. Consequently\, intensive research aiming to develop novel therapies promoting neuroplasticity are being carried out to allow rehabilitation of these patients. In this thesis\, we propose to combine novel neurorehabilitation and biofeedback strategies and to evaluate its therapeutic effect from a physiological (changes at muscular and neuroplastic level)\, kinematic and functional perspective with the objective of guiding the design of more successful and cost-effective therapies.
URL:https://ibecbarcelona.eu/event/phd-discussion-sessions-claudia-di-guglielmo-and-oiane-urra-2/
CATEGORIES:PhD Discussions Session
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Europe/Madrid:20150227T100000
DTEND;TZID=Europe/Madrid:20150227T110000
DTSTAMP:20260517T010650
CREATED:20150119T112620Z
LAST-MODIFIED:20150218T142126Z
UID:11716-1425031200-1425034800@ibecbarcelona.eu
SUMMARY:PhD Discussion Sessions: Silvia Pittolo and Noelia Campillo
DESCRIPTION:Alloswitch: an allosteric modulator for the control of a G protein-coupled receptor with light\nSilvia Pittolo\, Nanoprobes and nanoswitches group\nControlling drug activity with light offers the possibility of enhancing pharmacological selectivity with spatial and temporal regulation\, thus enabling highly localized therapeutic effects and precise dosing patterns. We have developed and characterized what is to our knowledge the first photoswitchable allosteric modulator of a G protein–coupled receptor. Alloswitch-1 is selective for the metabotropic glutamate receptor mGlu5 and enables the optical control of endogenous mGlu5 receptors. \n  \nEffect of cyclic hypoxia mimicking obstructive sleep apnea on the expression of hypoxia-inducible factor\nNoelia Campillo\, Cellular and respiratory biomechanics group\nIntermittent hypoxia (IH)\, the process whereby tissues are recurrently subjected to episodes of low oxygen tension\, is commonly associated with several pathological conditions\, such as obstructive sleep apnea (OSA) or cancer. Increasing evidence suggests that the cyclic frequency of IH is directly related to the emergence of OSA-associated morbidities. Although HIF-1 has been identified as the critical factor regulating cell responses to hypoxia\, the molecular mechanisms supporting the pathological effects of IH remain poorly understood. This is partly due to the lack of experimental systems to study the in vitro consequences of IH patterns realistically mimicking OSA. Here we present a simple polydimethylsiloxane (PDMS) chip aimed to apply high-frequency IH patterns and cyclic stretch simulating OSA at the cell level. The device was used to study the effect of different IH frequencies on HIF-1 expression.
URL:https://ibecbarcelona.eu/event/phd-discussion-sessions-silvia-pittolo-and-noelia-campillo/
CATEGORIES:PhD Discussions Session
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Europe/Madrid:20150227T100000
DTEND;TZID=Europe/Madrid:20150227T110000
DTSTAMP:20260517T010650
CREATED:20150119T112620Z
LAST-MODIFIED:20150119T112620Z
UID:95835-1425031200-1425034800@ibecbarcelona.eu
SUMMARY:PhD Discussion Sessions: Silvia Pittolo and Noelia Campillo
DESCRIPTION:Alloswitch: an allosteric modulator for the control of a G protein-coupled receptor with light\nSilvia Pittolo\, Nanoprobes and nanoswitches group\nControlling drug activity with light offers the possibility of enhancing pharmacological selectivity with spatial and temporal regulation\, thus enabling highly localized therapeutic effects and precise dosing patterns. We have developed and characterized what is to our knowledge the first photoswitchable allosteric modulator of a G protein–coupled receptor. Alloswitch-1 is selective for the metabotropic glutamate receptor mGlu5 and enables the optical control of endogenous mGlu5 receptors. \n  \nEffect of cyclic hypoxia mimicking obstructive sleep apnea on the expression of hypoxia-inducible factor\nNoelia Campillo\, Cellular and respiratory biomechanics group\nIntermittent hypoxia (IH)\, the process whereby tissues are recurrently subjected to episodes of low oxygen tension\, is commonly associated with several pathological conditions\, such as obstructive sleep apnea (OSA) or cancer. Increasing evidence suggests that the cyclic frequency of IH is directly related to the emergence of OSA-associated morbidities. Although HIF-1 has been identified as the critical factor regulating cell responses to hypoxia\, the molecular mechanisms supporting the pathological effects of IH remain poorly understood. This is partly due to the lack of experimental systems to study the in vitro consequences of IH patterns realistically mimicking OSA. Here we present a simple polydimethylsiloxane (PDMS) chip aimed to apply high-frequency IH patterns and cyclic stretch simulating OSA at the cell level. The device was used to study the effect of different IH frequencies on HIF-1 expression.
URL:https://ibecbarcelona.eu/event/phd-discussion-sessions-silvia-pittolo-and-noelia-campillo-2/
CATEGORIES:PhD Discussions Session
END:VEVENT
END:VCALENDAR