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DTSTART;TZID=Europe/Madrid:20191126T100000
DTEND;TZID=Europe/Madrid:20191126T120000
DTSTAMP:20260403T224630
CREATED:20191118T093609Z
LAST-MODIFIED:20191118T093609Z
UID:96557-1574762400-1574769600@ibecbarcelona.eu
SUMMARY:IBEC Seminar: Emilio Parisini
DESCRIPTION:Drugging the undruggable: towards the development of selective modulators of cadherin-mediated cell-cell adhesion\nEmilio Parisi\, Italian Institute of Technology (Milano) and Latvian Institute of Organic Synthesis (Riga) \nCadherins are transmembrane calcium-dependent cell adhesion proteins that mediate cellular adherens junction formation and tissue morphogenesis. Loss of cadherin-mediated adhesion has been implicated in many different steps of tumor progression such as invasion and migration\, and is strongly related to cell–cell detachment and metastasis. Altered expression profiles of epithelial E-cadherin (CDH1) and neuronal N-cadherin (CDH2) have often been observed in cancer cells\, most notably in the context of the epithelial-to-mesenchymal transition (EMT) process that occurs during cancer progression. Interestingly\, while in the majority of carcinomas E-cadherin is down-regulated\, in some epithelial ovarian cancer (EOC) cells are characterized by high expression levels of E-cadherin\, which facilitates EOC cell proliferation. So far\, structural and mutational studies have provided a rather detailed picture of the highly dynamic cadherin homo-dimerization mechanism. However\, because of this intrinsic dynamic behavior\, the rational design of small ligands targeting cadherin homophilic interactions has proved difficult. We determined the crystal structure of an E-cadherin extracellular fragment in complex with a peptidomimetic compound that partially inhibits cadherin homophilic adhesion. The structure\, which is the first and to date the only crystal structure of a cadherin extracellular portion in complex with a small molecule inhibitor\, reveals an unexpected binding mode and allows the identification of a druggable cadherin interface. Effective cell−cell adhesion modulators may represent potential anti-angiogenic drugs or pharmaceutical excipients to improve drug delivery across biological barriers. \nEmilio Parisini is a Group Leader at the Center for Nano Science and Technology of the Istituto Italiano di Tecnologia (IIT) in Milano. After completing a PhD in Chemistry at the University of Bologna (Italy)\, he worked at the Universities of Göttingen (Germany)\, Cambridge (UK) and Harvard (USA). In his laboratory\, he studies structure-function relationship in several chemical and biological systems\, focusing primarily on the study of the interactions between proteins and their ligands\, substrates or inhibitors. His current research interests include the functional and structural characterization of different members of the Cadherin family of cell adhesion proteins and of several different classes of enzymes for diagnostic (biosensors)\, therapeutic (structure-based drug design) and technological (protein and enzyme engineering) applications. \nHe has been invited to IBEC by Pau Gorostiza.
URL:https://ibecbarcelona.eu/event/ibec-seminar-emilio-parisi-4/
LOCATION:IBEC\, floor 11\, Tower I\, Baldiri Reixac 4-8\, 08028 Barcelona\, Spain
CATEGORIES:IBEC Seminar
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Europe/Madrid:20191126T100000
DTEND;TZID=Europe/Madrid:20191126T120000
DTSTAMP:20260403T224630
CREATED:20191118T093609Z
LAST-MODIFIED:20191119T141506Z
UID:69274-1574762400-1574769600@ibecbarcelona.eu
SUMMARY:IBEC Seminar: Emilio Parisini
DESCRIPTION:Drugging the undruggable: towards the development of selective modulators of cadherin-mediated cell-cell adhesion\nEmilio Parisi\, Italian Institute of Technology (Milano) and Latvian Institute of Organic Synthesis (Riga) \nCadherins are transmembrane calcium-dependent cell adhesion proteins that mediate cellular adherens junction formation and tissue morphogenesis. Loss of cadherin-mediated adhesion has been implicated in many different steps of tumor progression such as invasion and migration\, and is strongly related to cell–cell detachment and metastasis. Altered expression profiles of epithelial E-cadherin (CDH1) and neuronal N-cadherin (CDH2) have often been observed in cancer cells\, most notably in the context of the epithelial-to-mesenchymal transition (EMT) process that occurs during cancer progression. Interestingly\, while in the majority of carcinomas E-cadherin is down-regulated\, in some epithelial ovarian cancer (EOC) cells are characterized by high expression levels of E-cadherin\, which facilitates EOC cell proliferation. So far\, structural and mutational studies have provided a rather detailed picture of the highly dynamic cadherin homo-dimerization mechanism. However\, because of this intrinsic dynamic behavior\, the rational design of small ligands targeting cadherin homophilic interactions has proved difficult. We determined the crystal structure of an E-cadherin extracellular fragment in complex with a peptidomimetic compound that partially inhibits cadherin homophilic adhesion. The structure\, which is the first and to date the only crystal structure of a cadherin extracellular portion in complex with a small molecule inhibitor\, reveals an unexpected binding mode and allows the identification of a druggable cadherin interface. Effective cell−cell adhesion modulators may represent potential anti-angiogenic drugs or pharmaceutical excipients to improve drug delivery across biological barriers. \nEmilio Parisini is a Group Leader at the Center for Nano Science and Technology of the Istituto Italiano di Tecnologia (IIT) in Milano. After completing a PhD in Chemistry at the University of Bologna (Italy)\, he worked at the Universities of Göttingen (Germany)\, Cambridge (UK) and Harvard (USA). In his laboratory\, he studies structure-function relationship in several chemical and biological systems\, focusing primarily on the study of the interactions between proteins and their ligands\, substrates or inhibitors. His current research interests include the functional and structural characterization of different members of the Cadherin family of cell adhesion proteins and of several different classes of enzymes for diagnostic (biosensors)\, therapeutic (structure-based drug design) and technological (protein and enzyme engineering) applications. \nHe has been invited to IBEC by Pau Gorostiza.
URL:https://ibecbarcelona.eu/event/ibec-seminar-emilio-parisi/
LOCATION:IBEC\, floor 11\, Tower I\, Baldiri Reixac 4-8\, 08028 Barcelona\, Spain
CATEGORIES:IBEC Seminar
ORGANIZER;CN="IBEC":MAILTO:www.ibecbarcelona.eu
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Europe/Madrid:20191126T100000
DTEND;TZID=Europe/Madrid:20191126T120000
DTSTAMP:20260403T224630
CREATED:20191118T093609Z
LAST-MODIFIED:20191118T093609Z
UID:96552-1574762400-1574769600@ibecbarcelona.eu
SUMMARY:IBEC Seminar: Emilio Parisini
DESCRIPTION:Drugging the undruggable: towards the development of selective modulators of cadherin-mediated cell-cell adhesion\nEmilio Parisi\, Italian Institute of Technology (Milano) and Latvian Institute of Organic Synthesis (Riga) \nCadherins are transmembrane calcium-dependent cell adhesion proteins that mediate cellular adherens junction formation and tissue morphogenesis. Loss of cadherin-mediated adhesion has been implicated in many different steps of tumor progression such as invasion and migration\, and is strongly related to cell–cell detachment and metastasis. Altered expression profiles of epithelial E-cadherin (CDH1) and neuronal N-cadherin (CDH2) have often been observed in cancer cells\, most notably in the context of the epithelial-to-mesenchymal transition (EMT) process that occurs during cancer progression. Interestingly\, while in the majority of carcinomas E-cadherin is down-regulated\, in some epithelial ovarian cancer (EOC) cells are characterized by high expression levels of E-cadherin\, which facilitates EOC cell proliferation. So far\, structural and mutational studies have provided a rather detailed picture of the highly dynamic cadherin homo-dimerization mechanism. However\, because of this intrinsic dynamic behavior\, the rational design of small ligands targeting cadherin homophilic interactions has proved difficult. We determined the crystal structure of an E-cadherin extracellular fragment in complex with a peptidomimetic compound that partially inhibits cadherin homophilic adhesion. The structure\, which is the first and to date the only crystal structure of a cadherin extracellular portion in complex with a small molecule inhibitor\, reveals an unexpected binding mode and allows the identification of a druggable cadherin interface. Effective cell−cell adhesion modulators may represent potential anti-angiogenic drugs or pharmaceutical excipients to improve drug delivery across biological barriers. \nEmilio Parisini is a Group Leader at the Center for Nano Science and Technology of the Istituto Italiano di Tecnologia (IIT) in Milano. After completing a PhD in Chemistry at the University of Bologna (Italy)\, he worked at the Universities of Göttingen (Germany)\, Cambridge (UK) and Harvard (USA). In his laboratory\, he studies structure-function relationship in several chemical and biological systems\, focusing primarily on the study of the interactions between proteins and their ligands\, substrates or inhibitors. His current research interests include the functional and structural characterization of different members of the Cadherin family of cell adhesion proteins and of several different classes of enzymes for diagnostic (biosensors)\, therapeutic (structure-based drug design) and technological (protein and enzyme engineering) applications. \nHe has been invited to IBEC by Pau Gorostiza.
URL:https://ibecbarcelona.eu/event/ibec-seminar-emilio-parisi-2/
LOCATION:IBEC\, floor 11\, Tower I\, Baldiri Reixac 4-8\, 08028 Barcelona\, Spain
CATEGORIES:IBEC Seminar
ORGANIZER;CN="IBEC":MAILTO:www.ibecbarcelona.eu
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Europe/Madrid:20191126T100000
DTEND;TZID=Europe/Madrid:20191126T120000
DTSTAMP:20260403T224630
CREATED:20191118T093609Z
LAST-MODIFIED:20191118T093609Z
UID:96554-1574762400-1574769600@ibecbarcelona.eu
SUMMARY:IBEC Seminar: Emilio Parisini
DESCRIPTION:Drugging the undruggable: towards the development of selective modulators of cadherin-mediated cell-cell adhesion\nEmilio Parisi\, Italian Institute of Technology (Milano) and Latvian Institute of Organic Synthesis (Riga) \nCadherins are transmembrane calcium-dependent cell adhesion proteins that mediate cellular adherens junction formation and tissue morphogenesis. Loss of cadherin-mediated adhesion has been implicated in many different steps of tumor progression such as invasion and migration\, and is strongly related to cell–cell detachment and metastasis. Altered expression profiles of epithelial E-cadherin (CDH1) and neuronal N-cadherin (CDH2) have often been observed in cancer cells\, most notably in the context of the epithelial-to-mesenchymal transition (EMT) process that occurs during cancer progression. Interestingly\, while in the majority of carcinomas E-cadherin is down-regulated\, in some epithelial ovarian cancer (EOC) cells are characterized by high expression levels of E-cadherin\, which facilitates EOC cell proliferation. So far\, structural and mutational studies have provided a rather detailed picture of the highly dynamic cadherin homo-dimerization mechanism. However\, because of this intrinsic dynamic behavior\, the rational design of small ligands targeting cadherin homophilic interactions has proved difficult. We determined the crystal structure of an E-cadherin extracellular fragment in complex with a peptidomimetic compound that partially inhibits cadherin homophilic adhesion. The structure\, which is the first and to date the only crystal structure of a cadherin extracellular portion in complex with a small molecule inhibitor\, reveals an unexpected binding mode and allows the identification of a druggable cadherin interface. Effective cell−cell adhesion modulators may represent potential anti-angiogenic drugs or pharmaceutical excipients to improve drug delivery across biological barriers. \nEmilio Parisini is a Group Leader at the Center for Nano Science and Technology of the Istituto Italiano di Tecnologia (IIT) in Milano. After completing a PhD in Chemistry at the University of Bologna (Italy)\, he worked at the Universities of Göttingen (Germany)\, Cambridge (UK) and Harvard (USA). In his laboratory\, he studies structure-function relationship in several chemical and biological systems\, focusing primarily on the study of the interactions between proteins and their ligands\, substrates or inhibitors. His current research interests include the functional and structural characterization of different members of the Cadherin family of cell adhesion proteins and of several different classes of enzymes for diagnostic (biosensors)\, therapeutic (structure-based drug design) and technological (protein and enzyme engineering) applications. \nHe has been invited to IBEC by Pau Gorostiza.
URL:https://ibecbarcelona.eu/event/ibec-seminar-emilio-parisi-3/
LOCATION:IBEC\, floor 11\, Tower I\, Baldiri Reixac 4-8\, 08028 Barcelona\, Spain
CATEGORIES:IBEC Seminar
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Europe/Madrid:20191129T100000
DTEND;TZID=Europe/Madrid:20191129T120000
DTSTAMP:20260403T224630
CREATED:20191125T105531Z
LAST-MODIFIED:20191125T105531Z
UID:96561-1575021600-1575028800@ibecbarcelona.eu
SUMMARY:PhD Discussions Sessions: Ferran Velasco and Fabio Riefolo
DESCRIPTION:Cellulose-based cryogels for long-term culture of pancreatic islets and skeletal muscle tissue\nFerran Velasco\, Biosensors for Bioengineering\nIslet encapsulation inside traditional hydrogels is one of the most common techniques to study insulin secretion for Diabetes Mellitus studies. However\, it’s proved that cells encapsulated in a depth of more than 100 microns die due the lack of nutrient diffusion. As pancreatic islets are spherical aggregations of around 100 microns in diameter\, this problem increases exponentially. To solve this problem\, in this project we propose the use of new Carboxymethyl cellulose – gelatin biocomposite in combination with cryogelation technique to engineer a new in vitro model to mimic the insulin-mediated skeletal muscle glucose metabolism.\nCarboxymethyl cellulose (CMC) is biocompatible\, but not mammalian cell-degradable and shows extraordinary elasticity features. Gelatin is able to provide the 3D microenvironment for the proliferation of different cell types and cell-interactive biological activity\, very desirable properties for muscle and pancreas tissue scaffold. Cryogelation technique consists in freezing a prepolymer solution at sub-zero temperatures\, so water-ice crystals are formed while the material crosslinks. When it’s defrosted\, these water-ice crystals lead to “empty” cavities that forms a macroporous and very interconnected scaffold that fits with our needs of morphology and nutrient diffusion. \nWe first optimize the protocol to achieve the desired morphology; for the pancreatic tissue we achieved a random porosity with high interconnected pores and for the skeletal muscle we fabricate it with an anisotropic structure. We characterize it by stiffness\, pore distribution\, SEM images and swelling to know its mechanical properties. Then we seed cells in the specific cryogel to characterize its biological behavior depending the cryogel approach used. \nOur results are promising for seeding both cell types\, as the morphology and pore distributions fits with our needs. These scaffolds show higher nutrient diffusion\, good material properties and a better manipulation compared to traditional hydrogels for these tissues. \nPhotocontrol of Muscarinic Receptors and Applications In Vivo\nFabio Riefolo\, Nanoprobes and Nanoswitches\nRemote control of physiological functions with light offers the promise of unveiling their complex spatiotemporal dynamics in vivo\, and enabling highly focalized therapeutic interventions with reduced systemic toxicity. Optogenetic methods have been implemented in the heart\, but the need of genetic manipulation jeopardizes clinical applicability. We present a method to modulate cardiac function with light through a photoswitchable compound and without genetic manipulation. A new light-regulated drug\, named PAI\, was designed and synthesized to be active on M2 muscarinic acetylcholine receptor (mAChR). PAI can be reversibly photoisomerized between cis and trans conformations under UV and visible light and is able to photocontrol the activation M2 mAChRs in vitro. \nWe show that PAI has different light-dependent cardiac effects in a mammalian animal model. Finally\, we demonstrate the reversible\, real-time photocontrol of cardiac function in translucent wildtype tadpoles: PAI induced bradycardia and this effect could be reversibly switched using UV and visible illumination. PAI can also effectively activate M2 receptors using two-photon excitation with near-infrared light\, which overcomes the scattering and low penetration of short-wavelength illumination. Such a new approach may enable enhanced spatial and temporal selectivity for cardiovascular drugs.
URL:https://ibecbarcelona.eu/event/phd-discussions-sessions-ferran-velasco-and-fabio-riefolo-3/
LOCATION:IBEC\, floor 11\, Tower I\, Baldiri Reixac 4-8\, 08028 Barcelona\, Spain
CATEGORIES:PhD Discussions Session
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Europe/Madrid:20191129T100000
DTEND;TZID=Europe/Madrid:20191129T120000
DTSTAMP:20260403T224630
CREATED:20191125T105531Z
LAST-MODIFIED:20191125T105531Z
UID:69646-1575021600-1575028800@ibecbarcelona.eu
SUMMARY:PhD Discussions Sessions: Ferran Velasco and Fabio Riefolo
DESCRIPTION:Cellulose-based cryogels for long-term culture of pancreatic islets and skeletal muscle tissue\nFerran Velasco\, Biosensors for Bioengineering\nIslet encapsulation inside traditional hydrogels is one of the most common techniques to study insulin secretion for Diabetes Mellitus studies. However\, it’s proved that cells encapsulated in a depth of more than 100 microns die due the lack of nutrient diffusion. As pancreatic islets are spherical aggregations of around 100 microns in diameter\, this problem increases exponentially. To solve this problem\, in this project we propose the use of new Carboxymethyl cellulose – gelatin biocomposite in combination with cryogelation technique to engineer a new in vitro model to mimic the insulin-mediated skeletal muscle glucose metabolism.\nCarboxymethyl cellulose (CMC) is biocompatible\, but not mammalian cell-degradable and shows extraordinary elasticity features. Gelatin is able to provide the 3D microenvironment for the proliferation of different cell types and cell-interactive biological activity\, very desirable properties for muscle and pancreas tissue scaffold. Cryogelation technique consists in freezing a prepolymer solution at sub-zero temperatures\, so water-ice crystals are formed while the material crosslinks. When it’s defrosted\, these water-ice crystals lead to “empty” cavities that forms a macroporous and very interconnected scaffold that fits with our needs of morphology and nutrient diffusion. \nWe first optimize the protocol to achieve the desired morphology; for the pancreatic tissue we achieved a random porosity with high interconnected pores and for the skeletal muscle we fabricate it with an anisotropic structure. We characterize it by stiffness\, pore distribution\, SEM images and swelling to know its mechanical properties. Then we seed cells in the specific cryogel to characterize its biological behavior depending the cryogel approach used. \nOur results are promising for seeding both cell types\, as the morphology and pore distributions fits with our needs. These scaffolds show higher nutrient diffusion\, good material properties and a better manipulation compared to traditional hydrogels for these tissues. \nPhotocontrol of Muscarinic Receptors and Applications In Vivo\nFabio Riefolo\, Nanoprobes and Nanoswitches\nRemote control of physiological functions with light offers the promise of unveiling their complex spatiotemporal dynamics in vivo\, and enabling highly focalized therapeutic interventions with reduced systemic toxicity. Optogenetic methods have been implemented in the heart\, but the need of genetic manipulation jeopardizes clinical applicability. We present a method to modulate cardiac function with light through a photoswitchable compound and without genetic manipulation. A new light-regulated drug\, named PAI\, was designed and synthesized to be active on M2 muscarinic acetylcholine receptor (mAChR). PAI can be reversibly photoisomerized between cis and trans conformations under UV and visible light and is able to photocontrol the activation M2 mAChRs in vitro. \nWe show that PAI has different light-dependent cardiac effects in a mammalian animal model. Finally\, we demonstrate the reversible\, real-time photocontrol of cardiac function in translucent wildtype tadpoles: PAI induced bradycardia and this effect could be reversibly switched using UV and visible illumination. PAI can also effectively activate M2 receptors using two-photon excitation with near-infrared light\, which overcomes the scattering and low penetration of short-wavelength illumination. Such a new approach may enable enhanced spatial and temporal selectivity for cardiovascular drugs.
URL:https://ibecbarcelona.eu/event/phd-discussions-sessions-ferran-velasco-and-fabio-riefolo/
LOCATION:IBEC\, floor 11\, Tower I\, Baldiri Reixac 4-8\, 08028 Barcelona\, Spain
CATEGORIES:PhD Discussions Session
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Europe/Madrid:20191129T100000
DTEND;TZID=Europe/Madrid:20191129T120000
DTSTAMP:20260403T224630
CREATED:20191125T105531Z
LAST-MODIFIED:20191125T105531Z
UID:96558-1575021600-1575028800@ibecbarcelona.eu
SUMMARY:PhD Discussions Sessions: Ferran Velasco and Fabio Riefolo
DESCRIPTION:Cellulose-based cryogels for long-term culture of pancreatic islets and skeletal muscle tissue\nFerran Velasco\, Biosensors for Bioengineering\nIslet encapsulation inside traditional hydrogels is one of the most common techniques to study insulin secretion for Diabetes Mellitus studies. However\, it’s proved that cells encapsulated in a depth of more than 100 microns die due the lack of nutrient diffusion. As pancreatic islets are spherical aggregations of around 100 microns in diameter\, this problem increases exponentially. To solve this problem\, in this project we propose the use of new Carboxymethyl cellulose – gelatin biocomposite in combination with cryogelation technique to engineer a new in vitro model to mimic the insulin-mediated skeletal muscle glucose metabolism.\nCarboxymethyl cellulose (CMC) is biocompatible\, but not mammalian cell-degradable and shows extraordinary elasticity features. Gelatin is able to provide the 3D microenvironment for the proliferation of different cell types and cell-interactive biological activity\, very desirable properties for muscle and pancreas tissue scaffold. Cryogelation technique consists in freezing a prepolymer solution at sub-zero temperatures\, so water-ice crystals are formed while the material crosslinks. When it’s defrosted\, these water-ice crystals lead to “empty” cavities that forms a macroporous and very interconnected scaffold that fits with our needs of morphology and nutrient diffusion. \nWe first optimize the protocol to achieve the desired morphology; for the pancreatic tissue we achieved a random porosity with high interconnected pores and for the skeletal muscle we fabricate it with an anisotropic structure. We characterize it by stiffness\, pore distribution\, SEM images and swelling to know its mechanical properties. Then we seed cells in the specific cryogel to characterize its biological behavior depending the cryogel approach used. \nOur results are promising for seeding both cell types\, as the morphology and pore distributions fits with our needs. These scaffolds show higher nutrient diffusion\, good material properties and a better manipulation compared to traditional hydrogels for these tissues. \nPhotocontrol of Muscarinic Receptors and Applications In Vivo\nFabio Riefolo\, Nanoprobes and Nanoswitches\nRemote control of physiological functions with light offers the promise of unveiling their complex spatiotemporal dynamics in vivo\, and enabling highly focalized therapeutic interventions with reduced systemic toxicity. Optogenetic methods have been implemented in the heart\, but the need of genetic manipulation jeopardizes clinical applicability. We present a method to modulate cardiac function with light through a photoswitchable compound and without genetic manipulation. A new light-regulated drug\, named PAI\, was designed and synthesized to be active on M2 muscarinic acetylcholine receptor (mAChR). PAI can be reversibly photoisomerized between cis and trans conformations under UV and visible light and is able to photocontrol the activation M2 mAChRs in vitro. \nWe show that PAI has different light-dependent cardiac effects in a mammalian animal model. Finally\, we demonstrate the reversible\, real-time photocontrol of cardiac function in translucent wildtype tadpoles: PAI induced bradycardia and this effect could be reversibly switched using UV and visible illumination. PAI can also effectively activate M2 receptors using two-photon excitation with near-infrared light\, which overcomes the scattering and low penetration of short-wavelength illumination. Such a new approach may enable enhanced spatial and temporal selectivity for cardiovascular drugs.
URL:https://ibecbarcelona.eu/event/phd-discussions-sessions-ferran-velasco-and-fabio-riefolo-2/
LOCATION:IBEC\, floor 11\, Tower I\, Baldiri Reixac 4-8\, 08028 Barcelona\, Spain
CATEGORIES:PhD Discussions Session
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Europe/Madrid:20191129T100000
DTEND;TZID=Europe/Madrid:20191129T120000
DTSTAMP:20260403T224630
CREATED:20191125T105531Z
LAST-MODIFIED:20191125T105531Z
UID:96559-1575021600-1575028800@ibecbarcelona.eu
SUMMARY:PhD Discussions Sessions: Ferran Velasco and Fabio Riefolo
DESCRIPTION:Cellulose-based cryogels for long-term culture of pancreatic islets and skeletal muscle tissue\nFerran Velasco\, Biosensors for Bioengineering\nIslet encapsulation inside traditional hydrogels is one of the most common techniques to study insulin secretion for Diabetes Mellitus studies. However\, it’s proved that cells encapsulated in a depth of more than 100 microns die due the lack of nutrient diffusion. As pancreatic islets are spherical aggregations of around 100 microns in diameter\, this problem increases exponentially. To solve this problem\, in this project we propose the use of new Carboxymethyl cellulose – gelatin biocomposite in combination with cryogelation technique to engineer a new in vitro model to mimic the insulin-mediated skeletal muscle glucose metabolism.\nCarboxymethyl cellulose (CMC) is biocompatible\, but not mammalian cell-degradable and shows extraordinary elasticity features. Gelatin is able to provide the 3D microenvironment for the proliferation of different cell types and cell-interactive biological activity\, very desirable properties for muscle and pancreas tissue scaffold. Cryogelation technique consists in freezing a prepolymer solution at sub-zero temperatures\, so water-ice crystals are formed while the material crosslinks. When it’s defrosted\, these water-ice crystals lead to “empty” cavities that forms a macroporous and very interconnected scaffold that fits with our needs of morphology and nutrient diffusion. \nWe first optimize the protocol to achieve the desired morphology; for the pancreatic tissue we achieved a random porosity with high interconnected pores and for the skeletal muscle we fabricate it with an anisotropic structure. We characterize it by stiffness\, pore distribution\, SEM images and swelling to know its mechanical properties. Then we seed cells in the specific cryogel to characterize its biological behavior depending the cryogel approach used. \nOur results are promising for seeding both cell types\, as the morphology and pore distributions fits with our needs. These scaffolds show higher nutrient diffusion\, good material properties and a better manipulation compared to traditional hydrogels for these tissues. \nPhotocontrol of Muscarinic Receptors and Applications In Vivo\nFabio Riefolo\, Nanoprobes and Nanoswitches\nRemote control of physiological functions with light offers the promise of unveiling their complex spatiotemporal dynamics in vivo\, and enabling highly focalized therapeutic interventions with reduced systemic toxicity. Optogenetic methods have been implemented in the heart\, but the need of genetic manipulation jeopardizes clinical applicability. We present a method to modulate cardiac function with light through a photoswitchable compound and without genetic manipulation. A new light-regulated drug\, named PAI\, was designed and synthesized to be active on M2 muscarinic acetylcholine receptor (mAChR). PAI can be reversibly photoisomerized between cis and trans conformations under UV and visible light and is able to photocontrol the activation M2 mAChRs in vitro. \nWe show that PAI has different light-dependent cardiac effects in a mammalian animal model. Finally\, we demonstrate the reversible\, real-time photocontrol of cardiac function in translucent wildtype tadpoles: PAI induced bradycardia and this effect could be reversibly switched using UV and visible illumination. PAI can also effectively activate M2 receptors using two-photon excitation with near-infrared light\, which overcomes the scattering and low penetration of short-wavelength illumination. Such a new approach may enable enhanced spatial and temporal selectivity for cardiovascular drugs.
URL:https://ibecbarcelona.eu/event/phd-discussions-sessions-ferran-velasco-and-fabio-riefolo-2/
LOCATION:IBEC\, floor 11\, Tower I\, Baldiri Reixac 4-8\, 08028 Barcelona\, Spain
CATEGORIES:PhD Discussions Session
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Europe/Madrid:20191209T110000
DTEND;TZID=Europe/Madrid:20191209T130000
DTSTAMP:20260403T224630
CREATED:20191128T121521Z
LAST-MODIFIED:20191128T121521Z
UID:96565-1575889200-1575896400@ibecbarcelona.eu
SUMMARY:PhD Thesis Defence: Roberto Paoli
DESCRIPTION:Cell Culture interfaces for different organ-on-chip applications: from photolithography to rapid-prototyping techniques with sensor embedding\nRoberto Paoli\, Nanobioengineering group \n 
URL:https://ibecbarcelona.eu/event/phd-thesis-defence-roberto-paoli-3/
LOCATION:Facultat de Física – Sala de Graus Antiga
CATEGORIES:PhD Thesis Defence
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Europe/Madrid:20191209T110000
DTEND;TZID=Europe/Madrid:20191209T130000
DTSTAMP:20260403T224630
CREATED:20191128T121521Z
LAST-MODIFIED:20191128T121600Z
UID:69696-1575889200-1575896400@ibecbarcelona.eu
SUMMARY:PhD Thesis Defence: Roberto Paoli
DESCRIPTION:Cell Culture interfaces for different organ-on-chip applications: from photolithography to rapid-prototyping techniques with sensor embedding\nRoberto Paoli\, Nanobioengineering group \n 
URL:https://ibecbarcelona.eu/event/phd-thesis-defence-roberto-paoli/
LOCATION:Facultat de Física – Sala de Graus Antiga
CATEGORIES:PhD Thesis Defence
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Europe/Madrid:20191209T110000
DTEND;TZID=Europe/Madrid:20191209T130000
DTSTAMP:20260403T224630
CREATED:20191128T121521Z
LAST-MODIFIED:20191128T121521Z
UID:96564-1575889200-1575896400@ibecbarcelona.eu
SUMMARY:PhD Thesis Defence: Roberto Paoli
DESCRIPTION:Cell Culture interfaces for different organ-on-chip applications: from photolithography to rapid-prototyping techniques with sensor embedding\nRoberto Paoli\, Nanobioengineering group \n 
URL:https://ibecbarcelona.eu/event/phd-thesis-defence-roberto-paoli-3/
LOCATION:Facultat de Física – Sala de Graus Antiga
CATEGORIES:PhD Thesis Defence
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Europe/Madrid:20191209T110000
DTEND;TZID=Europe/Madrid:20191209T130000
DTSTAMP:20260403T224630
CREATED:20191128T121521Z
LAST-MODIFIED:20191128T121521Z
UID:96563-1575889200-1575896400@ibecbarcelona.eu
SUMMARY:PhD Thesis Defence: Roberto Paoli
DESCRIPTION:Cell Culture interfaces for different organ-on-chip applications: from photolithography to rapid-prototyping techniques with sensor embedding\nRoberto Paoli\, Nanobioengineering group \n 
URL:https://ibecbarcelona.eu/event/phd-thesis-defence-roberto-paoli-2/
LOCATION:Facultat de Física – Sala de Graus Antiga
CATEGORIES:PhD Thesis Defence
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Europe/Madrid:20191213T003000
DTEND;TZID=Europe/Madrid:20191213T140000
DTSTAMP:20260403T224630
CREATED:20191203T100159Z
LAST-MODIFIED:20191203T100159Z
UID:96567-1576197000-1576245600@ibecbarcelona.eu
SUMMARY:PhD Discussions Sessions: Maria Arista
DESCRIPTION:Super-resolution microscopy for understanding the formation and inhibition of influenza virus structures\nMaria Arista\, Nanoscopy for Nanomedicine\nSuper-resolution microscopy is a mighty tool that has the ability to study fluorescence samples beyond the diffraction limit\, achieving a spatial resolution around 20 nm. The study of viruses can greatly benefit from super-resolution imaging\, mainly due to their small size\, between 50 and 200 nm. Here we show that\, thanks to this technique\, we are able to visualize and study two relevant viral structures: filaments of influenza virus using stochastic optical construction microscopy (STORM) and virus-like particles formed from influenza using DNAPAINT (Points accumulation for imaging in nanoscale topography) .\nInfluenza A virus is highly pleomorphic\, and virions can have either spherical or filamentous morphology. Influenza A virus strain A/Udorn/72 (H3N2) produces copious amounts of long and thin filaments on the surface of infected cells\, led mainly by the matrix protein M1 and the membrane protein M2. These filaments are strongly related to the infectivity of influenza and cell-to-cell communication\, however\, due to the small size of these filaments (200 nm of width)\, they are hard to characterize in detail using immunofluorescence microscopy. \nHere\, we show with super-resolution microscopy that filament formation was inhibited by the treatment of cells with specific IgG2a and IgG1 antibodies but was not inhibited with the isotype control antibodies. Our results demonstrate that M2e-specific IgGs reduces the level influenza A virus replication in vitro and suggest that the inhibition of virus replication lead by M2especific antibodies is due to the fragmentation of filamentous virions and the loss of filament formation from the surfaces of infected cells.\nMoreover\, we study virus-like particles produced from influenza proteins transfected on mammalian cells. These structures mimic viruses but they lack viral genetic material\, for this reason they are great models to study influenza particles without risks. Influenza expresses 3 different proteins on the surface of the particle and the distribution and homogeneity between particles is not well understood. To study this distribution\, we are analyzing with DNA-PAINT the differential expression and distribution of these 3 proteins on the surface of the particles. Overall we show how super-resolution is suitable to study nanoscale viral structures and can provide new insights into anti-viral therapies. \n 
URL:https://ibecbarcelona.eu/event/phd-discussions-sessions-maria-arista-and-xarxa-quiroga-2/
LOCATION:IBEC\, floor 11\, Tower I\, Baldiri Reixac 4-8\, 08028 Barcelona\, Spain
CATEGORIES:PhD Discussions Session
ORGANIZER;CN="IBEC":MAILTO:www.ibecbarcelona.eu
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Europe/Madrid:20191213T003000
DTEND;TZID=Europe/Madrid:20191213T140000
DTSTAMP:20260403T224630
CREATED:20191203T100159Z
LAST-MODIFIED:20191203T100159Z
UID:96569-1576197000-1576245600@ibecbarcelona.eu
SUMMARY:PhD Discussions Sessions: Maria Arista
DESCRIPTION:Super-resolution microscopy as a powerful tool for understanding the formation and inhibition of influenza virus structures in mammalian cells\nMaria Arista\, Nanoscopy for Nanomedicine\nSuper-resolution microscopy is a mighty tool that has the ability to study fluorescence samples beyond the diffraction limit\, achieving a spatial resolution around 20 nm. The study of viruses can greatly benefit from super-resolution imaging\, mainly due to their small size\, between 50 and 200 nm. Here we show that\, thanks to this technique\, we are able to visualize and study two relevant viral structures: filaments of influenza virus using stochastic optical construction microscopy (STORM) and virus-like particles formed from influenza using DNAPAINT (Points accumulation for imaging in nanoscale topography) .\nInfluenza A virus is highly pleomorphic\, and virions can have either spherical or filamentous morphology. Influenza A virus strain A/Udorn/72 (H3N2) produces copious amounts of long and thin filaments on the surface of infected cells\, led mainly by the matrix protein M1 and the membrane protein M2. These filaments are strongly related to the infectivity of influenza and cell-to-cell communication\, however\, due to the small size of these filaments (200 nm of width)\, they are hard to characterize in detail using immunofluorescence microscopy. \nHere\, we show with super-resolution microscopy that filament formation was inhibited by the treatment of cells with specific IgG2a and IgG1 antibodies but was not inhibited with the isotype control antibodies. Our results demonstrate that M2e-specific IgGs reduces the level influenza A virus replication in vitro and suggest that the inhibition of virus replication lead by M2especific antibodies is due to the fragmentation of filamentous virions and the loss of filament formation from the surfaces of infected cells.\nMoreover\, we study virus-like particles produced from influenza proteins transfected on mammalian cells. These structures mimic viruses but they lack viral genetic material\, for this reason they are great models to study influenza particles without risks. Influenza expresses 3 different proteins on the surface of the particle and the distribution and homogeneity between particles is not well understood. To study this distribution\, we are analyzing with DNA-PAINT the differential expression and distribution of these 3 proteins on the surface of the particles. Overall we show how super-resolution is suitable to study nanoscale viral structures and can provide new insights into anti-viral therapies. \n 
URL:https://ibecbarcelona.eu/event/phd-discussions-sessions-maria-arista-and-xarxa-quiroga-3/
LOCATION:IBEC\, floor 11\, Tower I\, Baldiri Reixac 4-8\, 08028 Barcelona\, Spain
CATEGORIES:PhD Discussions Session
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Europe/Madrid:20191213T003000
DTEND;TZID=Europe/Madrid:20191213T140000
DTSTAMP:20260403T224630
CREATED:20191203T100159Z
LAST-MODIFIED:20191203T100159Z
UID:96571-1576197000-1576245600@ibecbarcelona.eu
SUMMARY:PhD Discussions Sessions: Maria Arista
DESCRIPTION:Super-resolution microscopy as a powerful tool for understanding the formation and inhibition of influenza virus structures in mammalian cells\nMaria Arista\, Nanoscopy for Nanomedicine\nSuper-resolution microscopy is a mighty tool that has the ability to study fluorescence samples beyond the diffraction limit\, achieving a spatial resolution around 20 nm. The study of viruses can greatly benefit from super-resolution imaging\, mainly due to their small size\, between 50 and 200 nm. Here we show that\, thanks to this technique\, we are able to visualize and study two relevant viral structures: filaments of influenza virus using stochastic optical construction microscopy (STORM) and virus-like particles formed from influenza using DNAPAINT (Points accumulation for imaging in nanoscale topography) .\nInfluenza A virus is highly pleomorphic\, and virions can have either spherical or filamentous morphology. Influenza A virus strain A/Udorn/72 (H3N2) produces copious amounts of long and thin filaments on the surface of infected cells\, led mainly by the matrix protein M1 and the membrane protein M2. These filaments are strongly related to the infectivity of influenza and cell-to-cell communication\, however\, due to the small size of these filaments (200 nm of width)\, they are hard to characterize in detail using immunofluorescence microscopy. \nHere\, we show with super-resolution microscopy that filament formation was inhibited by the treatment of cells with specific IgG2a and IgG1 antibodies but was not inhibited with the isotype control antibodies. Our results demonstrate that M2e-specific IgGs reduces the level influenza A virus replication in vitro and suggest that the inhibition of virus replication lead by M2especific antibodies is due to the fragmentation of filamentous virions and the loss of filament formation from the surfaces of infected cells.\nMoreover\, we study virus-like particles produced from influenza proteins transfected on mammalian cells. These structures mimic viruses but they lack viral genetic material\, for this reason they are great models to study influenza particles without risks. Influenza expresses 3 different proteins on the surface of the particle and the distribution and homogeneity between particles is not well understood. To study this distribution\, we are analyzing with DNA-PAINT the differential expression and distribution of these 3 proteins on the surface of the particles. Overall we show how super-resolution is suitable to study nanoscale viral structures and can provide new insights into anti-viral therapies. \n 
URL:https://ibecbarcelona.eu/event/phd-discussions-sessions-maria-arista-and-xarxa-quiroga-4/
LOCATION:IBEC\, floor 11\, Tower I\, Baldiri Reixac 4-8\, 08028 Barcelona\, Spain
CATEGORIES:PhD Discussions Session
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Europe/Madrid:20191213T003000
DTEND;TZID=Europe/Madrid:20191213T140000
DTSTAMP:20260403T224630
CREATED:20191203T100159Z
LAST-MODIFIED:20191210T093127Z
UID:69736-1576197000-1576245600@ibecbarcelona.eu
SUMMARY:PhD Discussions Sessions: Maria Arista
DESCRIPTION:Super-resolution microscopy for understanding the formation and inhibition of influenza virus structures\nMaria Arista\, Nanoscopy for Nanomedicine\nSuper-resolution microscopy is a mighty tool that has the ability to study fluorescence samples beyond the diffraction limit\, achieving a spatial resolution around 20 nm. The study of viruses can greatly benefit from super-resolution imaging\, mainly due to their small size\, between 50 and 200 nm. Here we show that\, thanks to this technique\, we are able to visualize and study two relevant viral structures: filaments of influenza virus using stochastic optical construction microscopy (STORM) and virus-like particles formed from influenza using DNAPAINT (Points accumulation for imaging in nanoscale topography) .\nInfluenza A virus is highly pleomorphic\, and virions can have either spherical or filamentous morphology. Influenza A virus strain A/Udorn/72 (H3N2) produces copious amounts of long and thin filaments on the surface of infected cells\, led mainly by the matrix protein M1 and the membrane protein M2. These filaments are strongly related to the infectivity of influenza and cell-to-cell communication\, however\, due to the small size of these filaments (200 nm of width)\, they are hard to characterize in detail using immunofluorescence microscopy. \nHere\, we show with super-resolution microscopy that filament formation was inhibited by the treatment of cells with specific IgG2a and IgG1 antibodies but was not inhibited with the isotype control antibodies. Our results demonstrate that M2e-specific IgGs reduces the level influenza A virus replication in vitro and suggest that the inhibition of virus replication lead by M2especific antibodies is due to the fragmentation of filamentous virions and the loss of filament formation from the surfaces of infected cells.\nMoreover\, we study virus-like particles produced from influenza proteins transfected on mammalian cells. These structures mimic viruses but they lack viral genetic material\, for this reason they are great models to study influenza particles without risks. Influenza expresses 3 different proteins on the surface of the particle and the distribution and homogeneity between particles is not well understood. To study this distribution\, we are analyzing with DNA-PAINT the differential expression and distribution of these 3 proteins on the surface of the particles. Overall we show how super-resolution is suitable to study nanoscale viral structures and can provide new insights into anti-viral therapies. \n 
URL:https://ibecbarcelona.eu/event/phd-discussions-sessions-maria-arista-and-xarxa-quiroga/
LOCATION:IBEC\, floor 11\, Tower I\, Baldiri Reixac 4-8\, 08028 Barcelona\, Spain
CATEGORIES:PhD Discussions Session
ORGANIZER;CN="IBEC":MAILTO:www.ibecbarcelona.eu
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Europe/Madrid:20191219T180000
DTEND;TZID=Europe/Madrid:20191219T180000
DTSTAMP:20260403T224630
CREATED:20191212T160639Z
LAST-MODIFIED:20191212T161322Z
UID:69861-1576778400-1576778400@ibecbarcelona.eu
SUMMARY:IBEC Christmas Celebration 2019
DESCRIPTION:We’re delighted to invite you once again to the best IBEC Christmas Party Ever!\n\nThursday 19th December · Fifteen Restaurant\, PCB\nAs usual\, there’ll be a chance to take part in a charity event to raise money for some very good causes. The more 1€ raffle tickets you buy during dinner\, the more likely you are to win one of the fabulous prizes provided by our sponsors. \nWith all this\, plus food\, drink\, music and some fun surprises\, the IBEC Christmas Party promises once again to be a fabulous way to kick-start the festive season. We’re counting on you to make the party go with a swing! \n18:00 – 20:00 Team building activity\n20:00 – 21:30 Sale of raffle tickets\n20:00 – 22:00 Buffet dinner\n22:00 Raffle and party\n00:30 End\n\nPlease register before 16th December here
URL:https://ibecbarcelona.eu/event/ibec-christmas-celebration-2019/
CATEGORIES:Other
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Europe/Madrid:20191219T180000
DTEND;TZID=Europe/Madrid:20191219T180000
DTSTAMP:20260403T224630
CREATED:20191212T160639Z
LAST-MODIFIED:20191212T160639Z
UID:96573-1576778400-1576778400@ibecbarcelona.eu
SUMMARY:IBEC Christmas Celebration 2019
DESCRIPTION:We’re delighted to invite you once again to the best IBEC Christmas Party Ever!\n\nThursday 19th December · Fifteen Restaurant\, PCB\nAs usual\, there’ll be a chance to take part in a charity event to raise money for some very good causes. The more 1€ raffle tickets you buy during dinner\, the more likely you are to win one of the fabulous prizes provided by our sponsors. \nWith all this\, plus food\, drink\, music and some fun surprises\, the IBEC Christmas Party promises once again to be a fabulous way to kick-start the festive season. We’re counting on you to make the party go with a swing! \n18:00 – 20:00 Team building activity\n20:00 – 21:30 Sale of raffle tickets\n20:00 – 22:00 Buffet dinner\n22:00 Raffle and party\n00:30 End\n\nPlease register before 16th December here
URL:https://ibecbarcelona.eu/event/ibec-christmas-celebration-2019-2/
CATEGORIES:Other
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Europe/Madrid:20191219T180000
DTEND;TZID=Europe/Madrid:20191219T180000
DTSTAMP:20260403T224630
CREATED:20191212T160639Z
LAST-MODIFIED:20191212T160639Z
UID:96575-1576778400-1576778400@ibecbarcelona.eu
SUMMARY:IBEC Christmas Celebration 2019
DESCRIPTION:We’re delighted to invite you once again to the best IBEC Christmas Party Ever!\n\nThursday 19th December · Fifteen Restaurant\, PCB\nAs usual\, there’ll be a chance to take part in a charity event to raise money for some very good causes. The more 1€ raffle tickets you buy during dinner\, the more likely you are to win one of the fabulous prizes provided by our sponsors. \nWith all this\, plus food\, drink\, music and some fun surprises\, the IBEC Christmas Party promises once again to be a fabulous way to kick-start the festive season. We’re counting on you to make the party go with a swing! \n18:00 – 20:00 Team building activity\n20:00 – 21:30 Sale of raffle tickets\n20:00 – 22:00 Buffet dinner\n22:00 Raffle and party\n00:30 End\nPlease register before 16th December here
URL:https://ibecbarcelona.eu/event/ibec-christmas-celebration-2019-3/
CATEGORIES:Other
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Europe/Madrid:20191219T180000
DTEND;TZID=Europe/Madrid:20191219T180000
DTSTAMP:20260403T224630
CREATED:20191212T160639Z
LAST-MODIFIED:20191212T160639Z
UID:96578-1576778400-1576778400@ibecbarcelona.eu
SUMMARY:IBEC Christmas Celebration 2019
DESCRIPTION:We’re delighted to invite you once again to the best IBEC Christmas Party Ever!\n\nThursday 19th December · Fifteen Restaurant\, PCB\nAs usual\, there’ll be a chance to take part in a charity event to raise money for some very good causes. The more 1€ raffle tickets you buy during dinner\, the more likely you are to win one of the fabulous prizes provided by our sponsors. \nWith all this\, plus food\, drink\, music and some fun surprises\, the IBEC Christmas Party promises once again to be a fabulous way to kick-start the festive season. We’re counting on you to make the party go with a swing! \n18:00 – 20:00 Team building activity\n20:00 – 21:30 Sale of raffle tickets\n20:00 – 22:00 Buffet dinner\n22:00 Raffle and party\n00:30 End\n\nPlease register before 16th December here
URL:https://ibecbarcelona.eu/event/ibec-christmas-celebration-2019-3/
CATEGORIES:Other
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Europe/Madrid:20200115T113000
DTEND;TZID=Europe/Madrid:20200115T130000
DTSTAMP:20260403T224630
CREATED:20200113T155752Z
LAST-MODIFIED:20200113T155752Z
UID:96590-1579087800-1579093200@ibecbarcelona.eu
SUMMARY:PhD Thesis Defence: Aida Garrido
DESCRIPTION:Optical control of endogenous receptors and cellular excitability with light\nAida Garrido\, Nanoprobes and nanoswitches group \nLight-controlled manipulation of neuronal activity has transformed the field of neurobiology. Light provides temporal and spatial resolution over activation or inhibition of targeted populations of neurons\, one single neuron or single synapses. Such precision could be achieved with optogenetics\, which is based on the over-expression of light-sensitive proteins\, but it requires gene therapy and often alters cellular physiology. An alternative to optogenetics is offered by photopharmacology (the development of light-regulated drugs or photoswitches)\, which can operate on endogenous receptors without genetic manipulation. Several new photoswitches are described in this thesis to improve their pharmacological and optical properties.\nIn photopharmacology\, azobenzene is the most commonly used light switch. Photoswitchable tethered ligands (PTLs) are tri-modular molecules able to anchor to target receptors and alter their function by switching the azobenzene group. In the first chapter we demonstrate the ability to target endogenous receptors of hippocampal neurons and olfactory bulb neurons from Xenopus larvae. The chemical strategy used was to introduce a highly reactive anchoring group to a PTL with similar structure to the reported MAG compound used in the light regulated glutamate receptor\, LiGluR. These new kind of PTLs were called “Targeted Covalent Photoswitches” (TCPs).\nThe promiscuity of the reactive group of TCP limits the possibility to spatially confine the drug conjugation. Thus\, we designed a photolabile TCP that can be conjugated to its target with a specific wavelength\, and then be photoswitched at another wavelength.\nDespite the advantages of all molecules described above\, they share a common limitation: the activation wavelength of azobenzene is in the UV-violet range. Increasing the push-pull effect at the azobenzenic core by adding different substituents\, we achieved a TCP derivative activatable at the visible range and ultrafast relaxing. In this way a single wavelength of stimulation can be used. Both features make them great candidates to control ultrafast neurotransmission processes such as the ones implicated in sound coding in the cochlea. We demonstrated in a gerbil animal model the capacity of this blue-activated TCP to photosensitize native receptors of adult gerbil cochlea. This first proof of concept opens new possibilities to develop optical cochlear implants for the treatment of hearing loss avoiding gene therapy.\nChemical substitutions can also be rationally designed to enhance two-photon (2P) absorptivity without modifying the dynamics of photoswitches. In the third chapter we described two new 2P enhanced MAG derivatives to photocontrol LiGluR. We validated their properties with an all-optical approach\, by recording calcium induced neuronal responses in organotypic hippocampal slices\, and in vivo in Caenorhabditis elegans. The combination of slow relaxation lifetime and enhanced 2P absorptivity is translated into an accumulation of the active isomer of the photoswitch that successfully enhances its functional effect even at low illumination power.\nAnother way to spectrally modify the characteristics of a chromophore while avoiding mutation screening and new synthetic processes is by using light-harvesting fluorophores. Spectral overlapping of fluorophore emission and chromophore absorption broadens its activation spectrum. By means of chemical protection and deprotection\, we orthogonally control the conjugation of fluorophores and photoswitches. This is the first demonstration of light-harvesting strategy for optogenetics and photopharmacology.\nFinally\, we describe the use of novel PCLs containing chromophores other than linear azobenzene for the light-controlled activation of hippocampal neurons. Cyclic azobenzenes reverse isomer thermal stability and consequently also the activity of the photoswitch. On the other hand\, stilbenes allow the irreversible but non-photo-destructive activation of the original molecule\, thus avoiding the creation of photo-products.\nIn conclusion\, this thesis puts forward several advances in the photochromism and pharmacology of photoswitches for the control of neurotransmission without need of genetic manipulation.
URL:https://ibecbarcelona.eu/event/phd-thesis-defence-aida-garrido-3/
LOCATION:Sala de Graus – Campus de Bellvitge
CATEGORIES:PhD Thesis Defence
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Europe/Madrid:20200115T113000
DTEND;TZID=Europe/Madrid:20200115T130000
DTSTAMP:20260403T224630
CREATED:20200113T155752Z
LAST-MODIFIED:20200113T160144Z
UID:70283-1579087800-1579093200@ibecbarcelona.eu
SUMMARY:PhD Thesis Defence: Aida Garrido
DESCRIPTION:Optical control of endogenous receptors and cellular excitability with light\nAida Garrido\, Nanoprobes and nanoswitches group \nLight-controlled manipulation of neuronal activity has transformed the field of neurobiology. Light provides temporal and spatial resolution over activation or inhibition of targeted populations of neurons\, one single neuron or single synapses. Such precision could be achieved with optogenetics\, which is based on the over-expression of light-sensitive proteins\, but it requires gene therapy and often alters cellular physiology. An alternative to optogenetics is offered by photopharmacology (the development of light-regulated drugs or photoswitches)\, which can operate on endogenous receptors without genetic manipulation. Several new photoswitches are described in this thesis to improve their pharmacological and optical properties.\nIn photopharmacology\, azobenzene is the most commonly used light switch. Photoswitchable tethered ligands (PTLs) are tri-modular molecules able to anchor to target receptors and alter their function by switching the azobenzene group. In the first chapter we demonstrate the ability to target endogenous receptors of hippocampal neurons and olfactory bulb neurons from Xenopus larvae. The chemical strategy used was to introduce a highly reactive anchoring group to a PTL with similar structure to the reported MAG compound used in the light regulated glutamate receptor\, LiGluR. These new kind of PTLs were called “Targeted Covalent Photoswitches” (TCPs).\nThe promiscuity of the reactive group of TCP limits the possibility to spatially confine the drug conjugation. Thus\, we designed a photolabile TCP that can be conjugated to its target with a specific wavelength\, and then be photoswitched at another wavelength.\nDespite the advantages of all molecules described above\, they share a common limitation: the activation wavelength of azobenzene is in the UV-violet range. Increasing the push-pull effect at the azobenzenic core by adding different substituents\, we achieved a TCP derivative activatable at the visible range and ultrafast relaxing. In this way a single wavelength of stimulation can be used. Both features make them great candidates to control ultrafast neurotransmission processes such as the ones implicated in sound coding in the cochlea. We demonstrated in a gerbil animal model the capacity of this blue-activated TCP to photosensitize native receptors of adult gerbil cochlea. This first proof of concept opens new possibilities to develop optical cochlear implants for the treatment of hearing loss avoiding gene therapy.\nChemical substitutions can also be rationally designed to enhance two-photon (2P) absorptivity without modifying the dynamics of photoswitches. In the third chapter we described two new 2P enhanced MAG derivatives to photocontrol LiGluR. We validated their properties with an all-optical approach\, by recording calcium induced neuronal responses in organotypic hippocampal slices\, and in vivo in Caenorhabditis elegans. The combination of slow relaxation lifetime and enhanced 2P absorptivity is translated into an accumulation of the active isomer of the photoswitch that successfully enhances its functional effect even at low illumination power.\nAnother way to spectrally modify the characteristics of a chromophore while avoiding mutation screening and new synthetic processes is by using light-harvesting fluorophores. Spectral overlapping of fluorophore emission and chromophore absorption broadens its activation spectrum. By means of chemical protection and deprotection\, we orthogonally control the conjugation of fluorophores and photoswitches. This is the first demonstration of light-harvesting strategy for optogenetics and photopharmacology.\nFinally\, we describe the use of novel PCLs containing chromophores other than linear azobenzene for the light-controlled activation of hippocampal neurons. Cyclic azobenzenes reverse isomer thermal stability and consequently also the activity of the photoswitch. On the other hand\, stilbenes allow the irreversible but non-photo-destructive activation of the original molecule\, thus avoiding the creation of photo-products.\nIn conclusion\, this thesis puts forward several advances in the photochromism and pharmacology of photoswitches for the control of neurotransmission without need of genetic manipulation.
URL:https://ibecbarcelona.eu/event/phd-thesis-defence-aida-garrido/
LOCATION:Sala de Graus – Campus de Bellvitge
CATEGORIES:PhD Thesis Defence
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Europe/Madrid:20200115T113000
DTEND;TZID=Europe/Madrid:20200115T130000
DTSTAMP:20260403T224630
CREATED:20200113T155752Z
LAST-MODIFIED:20200113T155752Z
UID:96586-1579087800-1579093200@ibecbarcelona.eu
SUMMARY:PhD Thesis Defence: Aida Garrido
DESCRIPTION:Control òptic de receptors endògens i excitabilitat cel·lular amb llum\nAida Garrido\, Nanoprobes and nanoswitches group \nL’estudi electrofisiològic i d’imatge de l’activitat neuronal en temps real requereix una gran resolució espacial i temporal. Ambdós trets es poden aconseguir amb la precisió\, velocitat i control remot que permet la llum. En aquesta tesi es descriu la caracterització de nous fotocommutadors dirigits a fotosensibilitzar receptors endògens de glutamat i estratègies per la millora de les seves característiques òptiques.\nEls primers fotoconmutadors descrits capaços de controlar amb llum l’activitat de canals endògens de glutamat son eficaços en altres aproximacions experimentals com ara en la inducció amb llum de potencials d’acció en neurones en cultiu\, en cultius organotípics d’hipocamp de rata o in vivo en larves de Xenopus electroporades\, a nivell d’un grup neuronal\, d’una única neurona o d’una única espina sinàptica. Modificant la part reactiva del fotocommutador\, som podem controlar espacialment la conjugació mitjançant patrons d’il·luminació.\nCanviant l’estructura química s’aconsegueix un fotocommutador actiu en el rang visible de llum i alhora extremadament ràpid. Fet que el fa ideal pel control de sinapsis ultra ràpides com ara les encarregades de codificar el so en la còclea. El disseny racional d’altres modificacions químiques en fotocommutadors de receptors de glutamat (LiGluRs) obre les portes al disseny de molècules sensibles a l’activació per 2 fotons. L’estimulació de 2 fotons amb làsers polsats de llum infraroja comporta importants avantatges com ara l’augment de la capacitat de penetració en teixit i una disminució de possibles danys degut a una sobreexposició a la llum. Característiques molt útils per estudis in vivo. Modificacions químiques dels fotocommutadors comporten altres modificacions en les seves característiques fotodinàmiques. Per aquest motiu\, hem desenvolupat una estratègia de conjugació de fluoròfors col·lectors de llum que mitjançant RET transfereixen la seva energia al cromòfor (fotocommutador)\, modificant-ne les característiques espectrals.\nEs demostra que cromòfors no conjugables permeten controlar l’activitat neuronal. Amb diversos avantatges respecte l’azobenzè clàssic: els azobenzens cíclics reverteix l’estabilitat i en conseqüència l’activitat de la molècula; i els estilbens permeten l’activació irreversible però no foto-destructiva de la molècula original\, evitant així la creació de foto-productes.\nEn conclusió\, en aquesta tesi es presenten noves molècules i estratègies de disseny per al control de la neurotransmissió sense necessitat de modificacions genètiques.
URL:https://ibecbarcelona.eu/event/phd-thesis-defence-aida-garrido-2/
LOCATION:Sala de Graus – Campus de Bellvitge
CATEGORIES:PhD Thesis Defence
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Europe/Madrid:20200115T113000
DTEND;TZID=Europe/Madrid:20200115T130000
DTSTAMP:20260403T224630
CREATED:20200113T155752Z
LAST-MODIFIED:20200113T155752Z
UID:96587-1579087800-1579093200@ibecbarcelona.eu
SUMMARY:PhD Thesis Defence: Aida Garrido
DESCRIPTION:Optical control of endogenous receptors and cellular excitability with light\nAida Garrido\, Nanoprobes and nanoswitches group \nLight-controlled manipulation of neuronal activity has transformed the field of neurobiology. Light provides temporal and spatial resolution over activation or inhibition of targeted populations of neurons\, one single neuron or single synapses. Such precision could be achieved with optogenetics\, which is based on the over-expression of light-sensitive proteins\, but it requires gene therapy and often alters cellular physiology. An alternative to optogenetics is offered by photopharmacology (the development of light-regulated drugs or photoswitches)\, which can operate on endogenous receptors without genetic manipulation. Several new photoswitches are described in this thesis to improve their pharmacological and optical properties.\nIn photopharmacology\, azobenzene is the most commonly used light switch. Photoswitchable tethered ligands (PTLs) are tri-modular molecules able to anchor to target receptors and alter their function by switching the azobenzene group. In the first chapter we demonstrate the ability to target endogenous receptors of hippocampal neurons and olfactory bulb neurons from Xenopus larvae. The chemical strategy used was to introduce a highly reactive anchoring group to a PTL with similar structure to the reported MAG compound used in the light regulated glutamate receptor\, LiGluR. These new kind of PTLs were called “Targeted Covalent Photoswitches” (TCPs).\nThe promiscuity of the reactive group of TCP limits the possibility to spatially confine the drug conjugation. Thus\, we designed a photolabile TCP that can be conjugated to its target with a specific wavelength\, and then be photoswitched at another wavelength.\nDespite the advantages of all molecules described above\, they share a common limitation: the activation wavelength of azobenzene is in the UV-violet range. Increasing the push-pull effect at the azobenzenic core by adding different substituents\, we achieved a TCP derivative activatable at the visible range and ultrafast relaxing. In this way a single wavelength of stimulation can be used. Both features make them great candidates to control ultrafast neurotransmission processes such as the ones implicated in sound coding in the cochlea. We demonstrated in a gerbil animal model the capacity of this blue-activated TCP to photosensitize native receptors of adult gerbil cochlea. This first proof of concept opens new possibilities to develop optical cochlear implants for the treatment of hearing loss avoiding gene therapy.\nChemical substitutions can also be rationally designed to enhance two-photon (2P) absorptivity without modifying the dynamics of photoswitches. In the third chapter we described two new 2P enhanced MAG derivatives to photocontrol LiGluR. We validated their properties with an all-optical approach\, by recording calcium induced neuronal responses in organotypic hippocampal slices\, and in vivo in Caenorhabditis elegans. The combination of slow relaxation lifetime and enhanced 2P absorptivity is translated into an accumulation of the active isomer of the photoswitch that successfully enhances its functional effect even at low illumination power.\nAnother way to spectrally modify the characteristics of a chromophore while avoiding mutation screening and new synthetic processes is by using light-harvesting fluorophores. Spectral overlapping of fluorophore emission and chromophore absorption broadens its activation spectrum. By means of chemical protection and deprotection\, we orthogonally control the conjugation of fluorophores and photoswitches. This is the first demonstration of light-harvesting strategy for optogenetics and photopharmacology.\nFinally\, we describe the use of novel PCLs containing chromophores other than linear azobenzene for the light-controlled activation of hippocampal neurons. Cyclic azobenzenes reverse isomer thermal stability and consequently also the activity of the photoswitch. On the other hand\, stilbenes allow the irreversible but non-photo-destructive activation of the original molecule\, thus avoiding the creation of photo-products.\nIn conclusion\, this thesis puts forward several advances in the photochromism and pharmacology of photoswitches for the control of neurotransmission without need of genetic manipulation.
URL:https://ibecbarcelona.eu/event/phd-thesis-defence-aida-garrido-2/
LOCATION:Sala de Graus – Campus de Bellvitge
CATEGORIES:PhD Thesis Defence
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Europe/Madrid:20200116T120000
DTEND;TZID=Europe/Madrid:20200116T140000
DTSTAMP:20260403T224630
CREATED:20200110T113019Z
LAST-MODIFIED:20200110T113019Z
UID:96583-1579176000-1579183200@ibecbarcelona.eu
SUMMARY:IBEC Seminar: Anotine Huet
DESCRIPTION:Cochlear optical stimulation of the auditory pathway\nAntoine Huet \, Institut für Auditorische Neurowissenschaften\, Universitätsmedizin Göttingen \nOptical stimulation of spiral ganglion neurons (SGNs) in the ear for coding sound information provides an interesting alternative to electrical stimulation that is used in current cochlear implants for hearing restoration. As light can be conveniently confined in space\, optical stimulation promises not only to increase the number of independent stimulation channels\, but also potentially to restore temporal response of SGNs which are phase-locked to the periodicity of the sound. Sensitizing SGNs to light can be achieved either by optogenetic manipulation for expressing a light-sensitive protein\, or by photopharmacological manipulation of receptors native to the cochlea. \nPioneering studies have established proof of principle of optical cochlear stimulation to restore hearing in rodent models of deafness (Hernandez et al\, 2014; Wrobel\, Dieter et al\, 2018). More recent work from the last 2 years have demonstrated i) improvement of frequency selectivity in response to light-over electrical stimuli (Dieter et al\, 2019)\, and ii) activation of the auditory pathway by faster opsins (i.e. shorter closing kinetics) which are more suitable for fast spiking SGNs (Mager\, Lopez de la Morena et al\, 2018\, Keppeler et al\, 2018). Finally\, ongoing work\, in collaboration with the team of Pau Gorostiza\, is showing than a photopharmacological approach targeting AMPA receptors could be used to optically activate the auditory pathway with performance similar to current optogenetic tools. \nDr. Antoine Huet is a postdoctoral fellow in the Institute for Auditory Neuroscience (University Medical Center Göttingen) under the supervision of Prof. Dr. Tobias Moser. Dr Huet received his BS degree in Audiology from the Institut libre Marie-Haps (Brussel\, Belgium) and MS degree in Audiology and auditory neuroscience from the University of Montpellier 1 (Montpellier\, France). He obtained his PhD degree in Chemical and biological science for Health from the University of Montpellier 1 (Montpellier\, France). He worked as a Postdoctoral fellow under the supervision of Pr. J-L. Puel and Jerome Bourien\, PhD in the Institut des neurosciences de Montpellier (INSERM U1051\, France) till May 2018. After\, he joined the laboratory of Pr. Dr. Tobias Moser in the Institute for Auditory Neuroscience from the University Medical Center Göttingen (Germany). His current research interest include the development of viral transfer of suitable ChR variants into spiral ganglion neurons and of multichannel optical stimulation using cochlear implants with arrays of microscale light emitting diodes and waveguides\, characterizing neuronal responses along the auditory pathway using physiological and behavioral methods. Thus\, to finally compare optogenetic to acoustic and electric stimulation. \nHe has been invited to IBEC by Pau Gorostiza
URL:https://ibecbarcelona.eu/event/ibec-seminar-anotine-huet-3/
LOCATION:Torre I Sala 3 Planta -1\, Carrer Baldiri Reixac 4-8\, Barcelona
CATEGORIES:IBEC Seminar
ORGANIZER;CN="IBEC":MAILTO:www.ibecbarcelona.eu
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Europe/Madrid:20200116T120000
DTEND;TZID=Europe/Madrid:20200116T140000
DTSTAMP:20260403T224630
CREATED:20200110T113019Z
LAST-MODIFIED:20200110T113019Z
UID:96580-1579176000-1579183200@ibecbarcelona.eu
SUMMARY:IBEC Seminar: Anotine Huet
DESCRIPTION:Cochlear optical stimulation of the auditory pathway\nAntoine Huet \, Institut für Auditorische Neurowissenschaften\, Universitätsmedizin Göttingen \nOptical stimulation of spiral ganglion neurons (SGNs) in the ear for coding sound information provides an interesting alternative to electrical stimulation that is used in current cochlear implants for hearing restoration. As light can be conveniently confined in space\, optical stimulation promises not only to increase the number of independent stimulation channels\, but also potentially to restore temporal response of SGNs which are phase-locked to the periodicity of the sound. Sensitizing SGNs to light can be achieved either by optogenetic manipulation for expressing a light-sensitive protein\, or by photopharmacological manipulation of receptors native to the cochlea. \nPioneering studies have established proof of principle of optical cochlear stimulation to restore hearing in rodent models of deafness (Hernandez et al\, 2014; Wrobel\, Dieter et al\, 2018). More recent work from the last 2 years have demonstrated i) improvement of frequency selectivity in response to light-over electrical stimuli (Dieter et al\, 2019)\, and ii) activation of the auditory pathway by faster opsins (i.e. shorter closing kinetics) which are more suitable for fast spiking SGNs (Mager\, Lopez de la Morena et al\, 2018\, Keppeler et al\, 2018). Finally\, ongoing work\, in collaboration with the team of Pau Gorostiza\, is showing than a photopharmacological approach targeting AMPA receptors could be used to optically activate the auditory pathway with performance similar to current optogenetic tools. \nDr. Antoine Huet is a postdoctoral fellow in the Institute for Auditory Neuroscience (University Medical Center Göttingen) under the supervision of Prof. Dr. Tobias Moser. Dr Huet received his BS degree in Audiology from the Institut libre Marie-Haps (Brussel\, Belgium) and MS degree in Audiology and auditory neuroscience from the University of Montpellier 1 (Montpellier\, France). He obtained his PhD degree in Chemical and biological science for Health from the University of Montpellier 1 (Montpellier\, France). He worked as a Postdoctoral fellow under the supervision of Pr. J-L. Puel and Jerome Bourien\, PhD in the Institut des neurosciences de Montpellier (INSERM U1051\, France) till May 2018. After\, he joined the laboratory of Pr. Dr. Tobias Moser in the Institute for Auditory Neuroscience from the University Medical Center Göttingen (Germany). His current research interest include the development of viral transfer of suitable ChR variants into spiral ganglion neurons and of multichannel optical stimulation using cochlear implants with arrays of microscale light emitting diodes and waveguides\, characterizing neuronal responses along the auditory pathway using physiological and behavioral methods. Thus\, to finally compare optogenetic to acoustic and electric stimulation. \nHe has been invited to IBEC by Pau Gorostiza
URL:https://ibecbarcelona.eu/event/ibec-seminar-anotine-huet-2/
LOCATION:Torre I Sala 3 Planta -1\, Carrer Baldiri Reixac 4-8\, Barcelona
CATEGORIES:IBEC Seminar
ORGANIZER;CN="IBEC":MAILTO:www.ibecbarcelona.eu
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Europe/Madrid:20200116T120000
DTEND;TZID=Europe/Madrid:20200116T140000
DTSTAMP:20260403T224630
CREATED:20200110T113019Z
LAST-MODIFIED:20200110T113032Z
UID:70211-1579176000-1579183200@ibecbarcelona.eu
SUMMARY:IBEC Seminar: Anotine Huet
DESCRIPTION:Cochlear optical stimulation of the auditory pathway\nAntoine Huet \, Institut für Auditorische Neurowissenschaften\, Universitätsmedizin Göttingen \nOptical stimulation of spiral ganglion neurons (SGNs) in the ear for coding sound information provides an interesting alternative to electrical stimulation that is used in current cochlear implants for hearing restoration. As light can be conveniently confined in space\, optical stimulation promises not only to increase the number of independent stimulation channels\, but also potentially to restore temporal response of SGNs which are phase-locked to the periodicity of the sound. Sensitizing SGNs to light can be achieved either by optogenetic manipulation for expressing a light-sensitive protein\, or by photopharmacological manipulation of receptors native to the cochlea. \nPioneering studies have established proof of principle of optical cochlear stimulation to restore hearing in rodent models of deafness (Hernandez et al\, 2014; Wrobel\, Dieter et al\, 2018). More recent work from the last 2 years have demonstrated i) improvement of frequency selectivity in response to light-over electrical stimuli (Dieter et al\, 2019)\, and ii) activation of the auditory pathway by faster opsins (i.e. shorter closing kinetics) which are more suitable for fast spiking SGNs (Mager\, Lopez de la Morena et al\, 2018\, Keppeler et al\, 2018). Finally\, ongoing work\, in collaboration with the team of Pau Gorostiza\, is showing than a photopharmacological approach targeting AMPA receptors could be used to optically activate the auditory pathway with performance similar to current optogenetic tools. \nDr. Antoine Huet is a postdoctoral fellow in the Institute for Auditory Neuroscience (University Medical Center Göttingen) under the supervision of Prof. Dr. Tobias Moser. Dr Huet received his BS degree in Audiology from the Institut libre Marie-Haps (Brussel\, Belgium) and MS degree in Audiology and auditory neuroscience from the University of Montpellier 1 (Montpellier\, France). He obtained his PhD degree in Chemical and biological science for Health from the University of Montpellier 1 (Montpellier\, France). He worked as a Postdoctoral fellow under the supervision of Pr. J-L. Puel and Jerome Bourien\, PhD in the Institut des neurosciences de Montpellier (INSERM U1051\, France) till May 2018. After\, he joined the laboratory of Pr. Dr. Tobias Moser in the Institute for Auditory Neuroscience from the University Medical Center Göttingen (Germany). His current research interest include the development of viral transfer of suitable ChR variants into spiral ganglion neurons and of multichannel optical stimulation using cochlear implants with arrays of microscale light emitting diodes and waveguides\, characterizing neuronal responses along the auditory pathway using physiological and behavioral methods. Thus\, to finally compare optogenetic to acoustic and electric stimulation. \nHe has been invited to IBEC by Pau Gorostiza
URL:https://ibecbarcelona.eu/event/ibec-seminar-anotine-huet/
LOCATION:Torre I Sala 3 Planta -1\, Carrer Baldiri Reixac 4-8\, Barcelona
CATEGORIES:IBEC Seminar
ORGANIZER;CN="IBEC":MAILTO:www.ibecbarcelona.eu
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Europe/Madrid:20200116T120000
DTEND;TZID=Europe/Madrid:20200116T140000
DTSTAMP:20260403T224630
CREATED:20200110T113019Z
LAST-MODIFIED:20200110T113019Z
UID:96581-1579176000-1579183200@ibecbarcelona.eu
SUMMARY:IBEC Seminar: Anotine Huet
DESCRIPTION:Cochlear optical stimulation of the auditory pathway\nAntoine Huet \, Institut für Auditorische Neurowissenschaften\, Universitätsmedizin Göttingen \nOptical stimulation of spiral ganglion neurons (SGNs) in the ear for coding sound information provides an interesting alternative to electrical stimulation that is used in current cochlear implants for hearing restoration. As light can be conveniently confined in space\, optical stimulation promises not only to increase the number of independent stimulation channels\, but also potentially to restore temporal response of SGNs which are phase-locked to the periodicity of the sound. Sensitizing SGNs to light can be achieved either by optogenetic manipulation for expressing a light-sensitive protein\, or by photopharmacological manipulation of receptors native to the cochlea. \nPioneering studies have established proof of principle of optical cochlear stimulation to restore hearing in rodent models of deafness (Hernandez et al\, 2014; Wrobel\, Dieter et al\, 2018). More recent work from the last 2 years have demonstrated i) improvement of frequency selectivity in response to light-over electrical stimuli (Dieter et al\, 2019)\, and ii) activation of the auditory pathway by faster opsins (i.e. shorter closing kinetics) which are more suitable for fast spiking SGNs (Mager\, Lopez de la Morena et al\, 2018\, Keppeler et al\, 2018). Finally\, ongoing work\, in collaboration with the team of Pau Gorostiza\, is showing than a photopharmacological approach targeting AMPA receptors could be used to optically activate the auditory pathway with performance similar to current optogenetic tools. \nDr. Antoine Huet is a postdoctoral fellow in the Institute for Auditory Neuroscience (University Medical Center Göttingen) under the supervision of Prof. Dr. Tobias Moser. Dr Huet received his BS degree in Audiology from the Institut libre Marie-Haps (Brussel\, Belgium) and MS degree in Audiology and auditory neuroscience from the University of Montpellier 1 (Montpellier\, France). He obtained his PhD degree in Chemical and biological science for Health from the University of Montpellier 1 (Montpellier\, France). He worked as a Postdoctoral fellow under the supervision of Pr. J-L. Puel and Jerome Bourien\, PhD in the Institut des neurosciences de Montpellier (INSERM U1051\, France) till May 2018. After\, he joined the laboratory of Pr. Dr. Tobias Moser in the Institute for Auditory Neuroscience from the University Medical Center Göttingen (Germany). His current research interest include the development of viral transfer of suitable ChR variants into spiral ganglion neurons and of multichannel optical stimulation using cochlear implants with arrays of microscale light emitting diodes and waveguides\, characterizing neuronal responses along the auditory pathway using physiological and behavioral methods. Thus\, to finally compare optogenetic to acoustic and electric stimulation. \nHe has been invited to IBEC by Pau Gorostiza
URL:https://ibecbarcelona.eu/event/ibec-seminar-anotine-huet-3/
LOCATION:Torre I Sala 3 Planta -1\, Carrer Baldiri Reixac 4-8\, Barcelona
CATEGORIES:IBEC Seminar
ORGANIZER;CN="IBEC":MAILTO:www.ibecbarcelona.eu
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Europe/Madrid:20200131T100000
DTEND;TZID=Europe/Madrid:20200131T120000
DTSTAMP:20260403T224630
CREATED:20200127T075750Z
LAST-MODIFIED:20200127T075810Z
UID:70739-1580464800-1580472000@ibecbarcelona.eu
SUMMARY:IBEC PhD Discussions Complementary Skills Session: Some philosophical & sociological issues about present science: from the privatization of science to the production of ignorance
DESCRIPTION:Some philosophical & sociological issues about present science: from the privatization of science to the production of ignorance\nEduard Aibar – Professor of Science and Technology Studies at the Arts & Humanities Department\, Universitat Oberta de Catalunya (UOC) \nThis seminar will present some basic points of the recent view of science produced by fields like the philosophy of science\, the sociology of science and the history of science. We will highlight the strong contrast between this view and the traditional view of science – still dominant in many areas of society – particularly around matters like the scientific method\, the evolution of science and the role of controversies. We will also discuss a few important phenomena of contemporary science: the productivist view of research evaluation\, the privatization of scientific knowledge and\, finally\, agnotology – the systematic production of ignorance. \nEduard Aibar is professor of Science and Technology Studies at the Arts & Humanities Department\, Universitat Oberta de Catalunya (UOC). He has been the director of the Internet Interdisciplinary Institute and former vice-president for research at UOC. He is currently leading a research group on Open Science and Innovation. His research has focused on the interaction between scientific and technological development and organizational and social change in areas such as eGovernment\, town planning and the Internet. He has recently led two research projects on Wikipedia and the public communication of science. \nFor more info see: https://www.uoc.edu/webs/eaibar
URL:https://ibecbarcelona.eu/event/ibec-phd-discussions-complementary-skills-session-some-philosophical-sociological-issues-about-present-science-from-the-privatization-of-science-to-the-production-of-ignorance/
LOCATION:IBEC\, floor 11\, Tower I\, Baldiri Reixac 4-8\, 08028 Barcelona\, Spain
CATEGORIES:PhD Discussions Complementary Skills Session
ORGANIZER;CN="IBEC":MAILTO:www.ibecbarcelona.eu
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Europe/Madrid:20200131T100000
DTEND;TZID=Europe/Madrid:20200131T120000
DTSTAMP:20260403T224630
CREATED:20200127T075750Z
LAST-MODIFIED:20200127T075750Z
UID:96596-1580464800-1580472000@ibecbarcelona.eu
SUMMARY:IBEC PhD Discussions Complementary Skills Session: Some philosophical & sociological issues about present science: from the privatization of science to the production of ignorance
DESCRIPTION:Some philosophical & sociological issues about present science: from the privatization of science to the production of ignorance\nEduard Aibar – Professor of Science and Technology Studies at the Arts & Humanities Department\, Universitat Oberta de Catalunya (UOC) \nThis seminar will present some basic points of the recent view of science produced by fields like the philosophy of science\, the sociology of science and the history of science. We will highlight the strong contrast between this view and the traditional view of science – still dominant in many areas of society – particularly around matters like the scientific method\, the evolution of science and the role of controversies. We will also discuss a few important phenomena of contemporary science: the productivist view of research evaluation\, the privatization of scientific knowledge and\, finally\, agnotology – the systematic production of ignorance. \nEduard Aibar is professor of Science and Technology Studies at the Arts & Humanities Department\, Universitat Oberta de Catalunya (UOC). He has been the director of the Internet Interdisciplinary Institute and former vice-president for research at UOC. He is currently leading a research group on Open Science and Innovation. His research has focused on the interaction between scientific and technological development and organizational and social change in areas such as eGovernment\, town planning and the Internet. He has recently led two research projects on Wikipedia and the public communication of science. \nFor more info see: https://www.uoc.edu/webs/eaibar
URL:https://ibecbarcelona.eu/event/ibec-phd-discussions-complementary-skills-session-some-philosophical-sociological-issues-about-present-science-from-the-privatization-of-science-to-the-production-of-ignorance-2/
LOCATION:IBEC\, floor 11\, Tower I\, Baldiri Reixac 4-8\, 08028 Barcelona\, Spain
CATEGORIES:PhD Discussions Complementary Skills Session
ORGANIZER;CN="IBEC":MAILTO:www.ibecbarcelona.eu
END:VEVENT
END:VCALENDAR