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DTSTART;TZID=Europe/Madrid:20251212T100000
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UID:129019-1765533600-1765537200@ibecbarcelona.eu
SUMMARY:Ibec Seminar. Anne de Poulpiquet
DESCRIPTION:CHARACTERIZATION OF ENZYMATIC BIOELECTRODES BY IN SITU FLUORESCENCE MICROSCOPY\nA. de Poulpiquet\,1 A. Guessab\, 1 H. M. Man\,1 I. Mazurenko\,1 L. Bouffier\,2 E. Lojou1 \n1Aix-Marseille Univ.\, CNRS\, Bioenergetics and Protein Engineering\, UMR 7281\, Marseille \n2 Institute of Molecular Sciences\, UMR CNRS 5255\, Univ. Bordeaux\, ENSMAC\, Pessac \nadepoulpiquet@imm.cnrs.fr \nRedox enzymes present remarkable catalytic properties (exceptional selectivity\, high kinetic constant\, low overvoltage\, etc.) which are particularly interesting for bio-electrochemical devices (biosensors\, biofuel cells\, bioreactors). In the latter\, they are immobilized at the surface of an electrode to enable electron transfer. Using three-dimensional (3D) electrodes improves the performance of the devices (sensitivity\, current densities). However\, enzymatic catalysis is very sensitive to the local environment (pH\, temperature\, ionic strength\, concentration of substrates\, products or inhibitors\, etc.) whose composition\, in the case of interfacial reactions\, can differ from the bulk of the solution. These disparities are exacerbated when the enzymes are confined in the pores of 3D electrodes\, due to the complexity of the associated mass transport. However\, electrochemistry only provides indirect information on the environment of the electrode. Therefore\, there is a major interest in coupling electrochemical techniques to other methods for collecting simultaneously spatial information.1-3 Precious information about mass transport and reactivity can be obtained by investigating the concentration profiles of the different species near the electrode surface\, or in the volume of a porous electrode. We show that in situ fluorescence confocal laser-scanning microscopy (FCLSM) coupled with electrochemistry enables investigation of redox enzyme reactivity involving the indirect generation of fluorogenic species.4\, 5 One of the most interesting features of FCLSM is the possibility to reconstruct 3D concentration profiles. Recording fluorescence in the volume adjacent to the electrode under potential control thus enables rebuilding the diffusion layer.2-5 We show that the method can be implemented to characterize electro-enzymatic catalysis at various planar and structured 3D electrodes.4\, 5 For example\, enzymatic O2 reduction involves proton transfers\, which was evidenced via the fluorescence change of strongly pH-dependent fluorophores. Local pH changes in the electrode plane were measured during O2 reduction catalyzed by an immobilized bilirubin oxidase. Moreover\, proton gradients generated during the enzymatic electrode reaction were imaged and their expansion under various experimental conditions were determined. Finally\, the method enabled direct imaging of the evolution of confined environments in porous 3D electrodes such as gas-diffusion layers during electro(enzymatic) catalysis.
URL:https://ibecbarcelona.eu/event/ibec-seminar-anne-de-poulpiquet/
LOCATION:Baobab room\, Floor 11\, Tower 1
CATEGORIES:IBEC Seminar
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DTSTART;TZID=Europe/Madrid:20251218T123000
DTEND;TZID=Europe/Madrid:20251218T140000
DTSTAMP:20260422T163004
CREATED:20251120T105724Z
LAST-MODIFIED:20251120T105724Z
UID:129847-1766061000-1766066400@ibecbarcelona.eu
SUMMARY:Ibec Seminar. Albert Folch
DESCRIPTION:“MICROFLUIDIC AND ROBOTIC PLATFORMS FOR TME-FRIENDLY CANCER DRUG EVALUATIONS”\nAlbert Folch\, Professor University of Washington\, Bioengineering Dept. \nThere is a lack of confidence in present in vitro disease models and drug efficacy tests\, as they do not properly recapitulate the dynamic physiology and pathophysiology of the human organism. This challenge is particularly acute in oncology: present tools to study drug responses fail to faithfully mimic the patient’s tumor microenvironment (TME) and thus have not kept up with tumor biology and drug testing needs. As a measure of this problem\, on average less than 4% of oncology drugs in clinical trials end up being FDA-approved\, a dismal approval rate that has dire social repercussions such as high cancer drug prices and difficult accessibility. We have developed a suite of microfluidic platforms that address this problem by multiplexing the delivery of drugs to intact-TME human biopsies\, altogether bypassing animal testing. We have developed and patented a microdissection methodology that allows for producing large numbers of cuboidal micro-tissues (“cuboids”) from a single tumor biopsy. We have been able to trap cuboids in arrays of microfluidic traps in a 96-well platform and we have developed very high-throughput automated robotic placement of mouse and human cuboids in 384-well plates. With these approaches\, it will soon be possible to bypass animal testing and perform direct testing of drugs using only human tumors. Since these new-generation tests preserve the TME intact\, we envision that they will minimize FDA failure rates and will contribute to alleviate the cost of cancer drugs. In this talk\, I will also cover innovative 3D printing approaches of general applicability to the fabrication of complex biomicrofluidic systems such as organs-on-chips. \nAlbert Folch’s lab works at the interface between microfluidics and cancer. He received both his BSc (1989) and PhD (1994) in Physics from the University of Barcelona (UB)\, Spain\, in 1989. During his Ph.D. he was a visiting scientist from 1990–91 at the Lawrence Berkeley Lab working on AFM/STM under Dr. Miquel Salmeron. From 1994–1996\, he was a postdoc at MIT developing MEMS under Martin Schmidt (EECS) and Mark Wrighton (Chemistry). In 1997\, he joined Mehmet Toner’s lab as a postdoc at Harvard-MGH to apply soft lithography to tissue engineering. He has been at Seattle’s UW BioE since June 2000\, where he is now a full Professor\, accumulating over 14\,850 citations (h-index 55; career average 150 citations/paper). In 25 years\, he has graduated 20 postdocs (20% of whom have reached faculty rank) and 33 graduate students (11 Ph.D. students\, 27% of whom faculty rank\, and 22 M.S. students). In 2001 he received an NSF Career Award\, and in 2014 he was elected to the AIMBE College of Fellows (Class of 2015). He serves on the Advisory Board of Lab on a Chip since 2010 and on the Editorial Board of Micromachines since 2019. In 2022 he was elected a member of the Institute for Catalan Studies\, one of the highest honors bestowed on Catalan scientists\, and his book “Hidden in Plain Sight” won winner of the 2022 Outstanding Academic Title Award by Choice. He is the author of 6 books (sole author)\, including Introduction to BioMEMS (2012\, Taylor&Francis)\, a textbook adopted by >103 departments in 18 countries\, Hidden in Plain Sight: The History\, Science\, and Engineering of Microfluidic Technology (MIT Press\, 2022)\, and the recent How the World Flows: Microfluidics from Raindrops to Covid Tests (Oxford University Press\, 2025). Since 2007\, the lab runs a celebrated outreach art program called BAIT (Bringing Art Into Technology)\, which has produced eight exhibits\, a popular resource gallery of >2\,000 free images related to microfluidics and microfabrication\, and a YouTube channel that plays microfluidic videos with music which accumulate >177\,000 visits since 2009.
URL:https://ibecbarcelona.eu/event/ibec-seminar-albert-folch-3/
LOCATION:Baobab room\, Floor 11\, Tower 1
CATEGORIES:IBEC Seminar
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