We recently developed the \u00b5LAS technology for nucleic acids processing. Its operating principle relies on the monitoring of DNA transport in a viscoelastic liquid under the combined action of hydrodynamic and electrophoretic forces (1). DNA molecules are dragged toward the walls of microchannels by a transverse force proportional to their contour length. Because the hydrodynamic decreases near the wall, DNA molecules are sorted according to their molecular weight. Furthermore, by tailoring the geometry of a channel with a constriction, we can tune the amplitude of transverse forces and stop molecules to design a concentrator that achieves enrichment rates of 100 to 1000 fold per minute.<\/p>\n
We will derive a quantitative model of DNA transport in \u00b5LAS that relies on 1 fitting parameter and perform rational optimizations of the technology. We will then exploit \u00b5LAS for sizing cell-free circulating DNA (cfDNA) in the blood (2), and demonstrate that cfDNA profiling is a promissing biomarker for the follow-up of cancer patients. Finally, we will present the principle of a DNA size-selective valve for the purification and sequencing of target genomic regions by combining \u00b5LAS with Cas9 endonuclease.<\/p>\n
(1) Ranchon et al., Lab Chip (2016)<\/em> \u00b5LAS technology for DNA processing: setting up elementary functions (concentration, separation, purification, identification) and application in oncology and targeted sequencing<\/p>","slug":"ibec-seminar-aurelien-bancaud-2","image":false,"all_day":false,"start_date":"2018-03-28 10:00:00","start_date_details":{"year":"2018","month":"03","day":"28","hour":"10","minutes":"00","seconds":"00"},"end_date":"2018-03-28 11:00:00","end_date_details":{"year":"2018","month":"03","day":"28","hour":"11","minutes":"00","seconds":"00"},"utc_start_date":"2018-03-28 08:00:00","utc_start_date_details":{"year":"2018","month":"03","day":"28","hour":"08","minutes":"00","seconds":"00"},"utc_end_date":"2018-03-28 09:00:00","utc_end_date_details":{"year":"2018","month":"03","day":"28","hour":"09","minutes":"00","seconds":"00"},"timezone":"Europe\/Madrid","timezone_abbr":"CEST","cost":"","cost_details":{"currency_symbol":"","currency_code":"","currency_position":"prefix","values":[]},"website":"","show_map":false,"show_map_link":false,"hide_from_listings":false,"sticky":false,"featured":false,"categories":[{"name":"IBEC Seminar","slug":"ibec-seminar","term_group":0,"term_taxonomy_id":108,"taxonomy":"tribe_events_cat","description":"","parent":0,"count":341,"filter":"raw","term_order":"0","id":105,"urls":{"self":"https:\/\/ibecbarcelona.eu\/es\/wp-json\/tribe\/events\/v1\/categories\/105","collection":"https:\/\/ibecbarcelona.eu\/es\/wp-json\/tribe\/events\/v1\/categories"}}],"tags":[],"venue":{"id":27435,"author":"4","status":"publish","date":"2017-02-03 11:34:17","date_utc":"2017-02-03 10:34:17","modified":"2017-02-03 11:34:17","modified_utc":"2017-02-03 10:34:17","url":"https:\/\/ibecbarcelona.eu\/recinto\/ibec-floor-11-tower-i-11\/","venue":"IBEC, floor 11, Tower I","slug":"ibec-floor-11-tower-i-11","address":"Baldiri Reixac 4-8","city":"08028 Barcelona","country":"Spain","json_ld":{"@type":"Place","name":"IBEC, floor 11, Tower I","description":"","url":"","address":{"@type":"PostalAddress","streetAddress":"Baldiri Reixac 4-8","addressLocality":"08028 Barcelona","addressCountry":"Spain"},"telephone":"","sameAs":""},"show_map":true,"show_map_link":true,"global_id":"ibecbarcelona.eu\/es\/?id=27435","global_id_lineage":["ibecbarcelona.eu\/es\/?id=27435"]},"organizer":[],"custom_fields":[],"json_ld":{"@context":"http:\/\/schema.org","@type":"Event","name":"IBEC Seminar: Aur\u00e9lien Bancaud","description":"<p>\u00b5LAS technology for DNA processing: setting up elementary functions (concentration, separation, purification, identification) and application in oncology and targeted sequencing<\/p>\\n","url":"https:\/\/ibecbarcelona.eu\/es\/event\/ibec-seminar-aurelien-bancaud-2\/","eventAttendanceMode":"https:\/\/schema.org\/OfflineEventAttendanceMode","eventStatus":"https:\/\/schema.org\/EventScheduled","startDate":"2018-03-28T10:00:00+02:00","endDate":"2018-03-28T11:00:00+02:00","location":{"@type":"Place","name":"IBEC, floor 11, Tower I","description":"","url":"","address":{"@type":"PostalAddress","streetAddress":"Baldiri Reixac 4-8","addressLocality":"08028 Barcelona","addressCountry":"Spain"},"telephone":"","sameAs":""},"performer":"Organization"}}
\n (2) Andriamanampisoa et al., Anal Chem (2018)<\/em><\/p>","excerpt":"