{"id":119956,"date":"2024-07-29T10:49:21","date_gmt":"2024-07-29T08:49:21","guid":{"rendered":"https:\/\/ibecbarcelona.eu\/new-bioink-to-mimic-human-skin-constructs-using-3d-bioprinting\/"},"modified":"2024-09-02T15:44:09","modified_gmt":"2024-09-02T13:44:09","slug":"nueva-biotinta-para-sintetizar-piel-humana-mediante-bioimpresion-3d","status":"publish","type":"post","link":"https:\/\/ibecbarcelona.eu\/es\/nueva-biotinta-para-sintetizar-piel-humana-mediante-bioimpresion-3d\/","title":{"rendered":"Nueva biotinta para sintetizar piel humana mediante bioimpresi\u00f3n 3D\u00a0\u00a0"},"content":{"rendered":"\n<h4 class=\"wp-block-heading\">La demanda de tejidos de piel sintetizados en laboratorio ha aumentado tanto para aplicaciones<em> in vitro<\/em> como <em>in vivo<\/em>, siendo uno de los puntos clave del proceso la correcta elecci\u00f3n del material del \u201candamio\u201d sobre el que se construir\u00e1 el tejido. En este escenario, investigadoras del IBEC acaban de publicar un trabajo que describe una nueva biotinta dise\u00f1ada para la construir piel humana a trav\u00e9s de ingenier\u00eda de tejidos utilizando hidrogeles basados en norborneno-pululano.&nbsp;<\/h4>\n\n\n\n<figure class=\"wp-block-image size-large\"><img decoding=\"async\" width=\"1024\" height=\"768\" data-src=\"https:\/\/ibecbarcelona.eu\/wp-content\/uploads\/2024\/07\/image-2-1024x768.jpeg\" alt=\"\" class=\"wp-image-119949 lazyload\" data-srcset=\"https:\/\/ibecbarcelona.eu\/wp-content\/uploads\/2024\/07\/image-2-1024x768.jpeg 1024w, https:\/\/ibecbarcelona.eu\/wp-content\/uploads\/2024\/07\/image-2-300x225.jpeg 300w, https:\/\/ibecbarcelona.eu\/wp-content\/uploads\/2024\/07\/image-2-768x576.jpeg 768w, https:\/\/ibecbarcelona.eu\/wp-content\/uploads\/2024\/07\/image-2.jpeg 1143w\" data-sizes=\"(max-width: 1024px) 100vw, 1024px\" src=\"data:image\/svg+xml;base64,PHN2ZyB3aWR0aD0iMSIgaGVpZ2h0PSIxIiB4bWxucz0iaHR0cDovL3d3dy53My5vcmcvMjAwMC9zdmciPjwvc3ZnPg==\" style=\"--smush-placeholder-width: 1024px; --smush-placeholder-aspect-ratio: 1024\/768;\" \/><figcaption class=\"wp-element-caption\">Investigadoras del grupo de Sistemas Biomim\u00e9ticos para Ingenier\u00eda Celular del IBEC (de izquierda a derecha): Angela Cirulli, N\u00faria Torras y Elena Mart\u00ednez.&nbsp;<\/figcaption><\/figure>\n\n\n\n<p>Los tejidos cut\u00e1neos obtenidos por bioingenier\u00eda tienen aplicaciones vers\u00e1tiles en entornos cl\u00ednicos, como la sustituci\u00f3n de piel y los ap\u00f3sitos avanzados para heridas, as\u00ed como en escenarios precl\u00ednicos para el testeo de nuevos f\u00e1rmacos, el desarrollo de modelos de enfermedades y ensayos con cosm\u00e9ticos. Un tejido de piel sintetizado mediante bioingenier\u00eda debe ser f\u00e1cil de preparar, resistente mec\u00e1nicamente y propicio para la adhesi\u00f3n y el crecimiento celular, tanto en el compartimento epid\u00e9rmico como en el d\u00e9rmico. Debe lograr un equilibrio entre la elasticidad y la estabilidad mec\u00e1nica, y a la vez, proporcionar un microambiente celular adecuado para contener los diferentes compartimentos de la piel humana.&nbsp;&nbsp;<\/p>\n\n\n\n<p>Recientemente, investigadoras lideradas por Elena Mart\u00ednez del grupo de Sistemas Biomim\u00e9ticos para Ingenier\u00eda Celular del IBEC, han publicado en la revista International Journal of Bioprinting el trabajo \u00ab<a href=\"https:\/\/accscience.com\/journal\/IJB\/0\/0\/10.36922\/ijb.3395\" target=\"_blank\" rel=\"noreferrer noopener\">Mimicing human skin constructs using norbornene-pullulan-based hydrogels<\/a>\u00ab, que ayudar\u00e1 a abordar y resolver estas cuestiones. En concreto, presentaron una nueva biotinta entrecruzable mediante luz, dise\u00f1ada para sintetizar piel humana, basada en formulaciones de tiol-norborneno-pululano (N-PLN) combinadas con varios fotopolimerizadores.\u00a0Este trabajo se ha desarrollado en el marco de el proyecto europeo B-BRIGHTER (No. 101057894).<\/p>\n\n\n\n<p>Esta nueva biotinta se utiliz\u00f3 de manera efectiva en una bioimpresora de escritura por l\u00e1ser desarrollada en el laboratorio, para generar constructos d\u00e9rmicos epitelializados. El compartimento d\u00e9rmico se form\u00f3 mediante la fotopolimerizaci\u00f3n de una soluci\u00f3n de pre-pol\u00edmero que conten\u00eda fibroblastos humanos, mientras que el compartimento epid\u00e9rmico se desarroll\u00f3 sembrando queratinocitos humanos en los hidrogeles previamente generados con fibroblastos embebidos. Utilizando luz visible, se pudieron imprimir hidrogeles de 2,5 mm\u00b3 con c\u00e9lulas en su interior, en solo 10 segundos. La qu\u00edmica de fotopolimerizaci\u00f3n mediante grupos thiol empleada en este estudio cre\u00f3 una matriz extracelular bien definida con reticulaciones ortogonales, manteniendo altas tasas de viabilidad celular para fibroblastos encapsulados.&nbsp;&nbsp;<\/p>\n\n\n\n<p>Este estudio supone una estrategia prometedora para desarrollar un modelo de piel humana utilizando un sistema de bioimpresi\u00f3n 3D basado en luz hecho a medida y usando nuevos pol\u00edmeros N-PLN. Estos hidrogeles, formados despu\u00e9s de una breve exposici\u00f3n a la luz visible a baja dosis, exhiben las propiedades fisicoqu\u00edmicas necesarias para proporcionar una excelente viabilidad, proliferaci\u00f3n y elongaci\u00f3n celular, as\u00ed como la secreci\u00f3n de prote\u00ednas de matriz extracelular, cruciales para la correcta formaci\u00f3n de los tejidos. Adem\u00e1s, los hidrogeles con fibroblastos embebidos apoyan la adhesi\u00f3n y crecimiento de los queratinocitos encima, lo que permite la formaci\u00f3n de constructos d\u00e9rmicos epitelializados.&nbsp;<\/p>\n\n\n\n<p>Este m\u00e9todo representa un prometedor punto de partida para el desarrollo de modelos de piel humana basadas en hidrogeles fotopolimerizables utilizando la qu\u00edmica de tiol-norborneno, abriendo camino a la creaci\u00f3n de complejos modelos <em>in<\/em> <em>vitro<\/em> de tejidos humanos. La combinaci\u00f3n de la fotopolimerizaci\u00f3n mediante luz visible con materiales basados en N-PLN, representa una clara alternativa para el desarrollo modelos de piel que sean f\u00e1ciles de usar y r\u00e1pidos de sintetizar, reproducibles y econ\u00f3micamente rentables, lo que podr\u00eda ser particularmente beneficioso para los ensayos precl\u00ednicos <em>in vitro<\/em> en investigaci\u00f3n cosm\u00e9tica y farmac\u00e9utica.&nbsp;<\/p>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n\n\n\n<p><strong>Art\u00edculo de referencia:<\/strong><\/p>\n\n\n\n<p>Angela Cirulli, Livia Neves Borgheti-Cardoso, N\u00faria Torras, Elena Mart\u00ednez. <strong>Mimicking human skin constructs using norbornene-pullulan-based hydrogels. <\/strong><em>International Journal of Bioprinting<\/em> (2024). DOI: <a href=\"https:\/\/doi.org\/10.36922\/ijb.3395\" target=\"_blank\" rel=\"noreferrer noopener\">https:\/\/doi.org\/10.36922\/ijb.3395<\/a>\u00a0<\/p>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n","protected":false},"excerpt":{"rendered":"<p>La demanda de tejidos de piel sintetizados en laboratorio ha aumentado tanto para aplicaciones in vitro como in vivo, siendo uno de los puntos clave del proceso la correcta elecci\u00f3n &#8230; <a title=\"Nueva biotinta para sintetizar piel humana mediante bioimpresi\u00f3n 3D\u00a0\u00a0\" class=\"read-more\" href=\"https:\/\/ibecbarcelona.eu\/es\/nueva-biotinta-para-sintetizar-piel-humana-mediante-bioimpresion-3d\/\" aria-label=\"More on Nueva biotinta para sintetizar piel humana mediante bioimpresi\u00f3n 3D\u00a0\u00a0\">Read more<\/a><\/p>\n","protected":false},"author":4,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[97],"tags":[1770,1827,825],"post_formats":[],"class_list":["post-119956","post","type-post","status-publish","format-standard","hentry","category-noticias-de-investigacion","tag-bioimpresion-3d-es","tag-biomimetic-systems-for-cell-engineering-es","tag-elena-martinez-es"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.4 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Nueva biotinta para sintetizar piel humana mediante bioimpresi\u00f3n 3D\u00a0\u00a0 - Institute for Bioengineering of Catalonia<\/title>\n<meta name=\"description\" content=\"investigadoras del IBEC acaban de publicar un trabajo que describe una nueva biotinta dise\u00f1ada para la construir piel humana a trav\u00e9s de ingenier\u00eda de tejidos utilizando hidrogeles basados en 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