{"id":60938,"date":"2018-08-21T13:54:09","date_gmt":"2018-08-21T11:54:09","guid":{"rendered":"http:\/\/ibecbarcelona.eu\/?p=60938"},"modified":"2018-09-03T15:33:44","modified_gmt":"2018-09-03T13:33:44","slug":"mejores-modelos-in-vitro-para-estudiar-el-intestino-humano","status":"publish","type":"post","link":"https:\/\/ibecbarcelona.eu\/es\/mejores-modelos-in-vitro-para-estudiar-el-intestino-humano\/","title":{"rendered":"Mejores modelos in vitro para estudiar el intestino humano"},"content":{"rendered":"
Imagen: Representaci\u00f3n esquem\u00e1tica de la diferenciaci\u00f3n in vitro del tejido intestinal de las c\u00e9lulas madre pluripotentes.<\/h6>\n

Estos se han utilizado para estudios de absorci\u00f3n y metabolismo de f\u00e1rmacos desde la d\u00e9cada de 1980. <\/p>\n

Sin embargo, los modelos actuales, que usan c\u00e9lulas Caco-2 derivadas de tumores intestinales humanos, no son completamente representativos del intestino delgado humano.<\/p>\n

El art\u00edculo sugiere usar iPSCs (del ingl\u00e9s induced Pluripotent Stem Cells) y diferenciarlas para que se conviertan en c\u00e9lulas similares a las intestinales, con el fin de desarrollar modelos que proporcionen mejores predicciones del comportamiento de los nuevos f\u00e1rmacos. \u00abLas c\u00e9lulas similares a los enterocitos obtenidas de iPSCs tienen m\u00e1s similitudes con los enterocitos primarios que Caco-2, que es el ‘est\u00e1ndar de oro’ actual\u00bb, dice la primera autora Helena Macedo, estudiante de doctorado de Porto, co-supervisada por Elena. \u00abUsar estas c\u00e9lulas en modelos intestinales puede permitir una correlaci\u00f3n m\u00e1s fiable con la situaci\u00f3n que se da in vivo<\/em>\u00ab.<\/p>\n

Sin embargo, a\u00fan quedan varios obst\u00e1culos que salvar. Si bien es posible obtener c\u00e9lulas de tipo enterocito a partir de iPSCs, el porcentaje de c\u00e9lulas inmaduras sigue siendo alto, lo que indica que los protocolos de diferenciaci\u00f3n actuales no producen una poblaci\u00f3n con el nivel requerido de homogeneidad. Adem\u00e1s, mientras que las c\u00e9lulas de tipo enterocito derivadas de iPSC muestran una mayor expresi\u00f3n de la mayor\u00eda de los transportadores y enzimas que las c\u00e9lulas Caco-2, todav\u00eda est\u00e1n lejos de los niveles habituales in vivo<\/em>. Los costes y el tiempo asociados con la diferenciaci\u00f3n de iPSCs tambi\u00e9n suponen desventajas.<\/p>\n

\u00abPara que las iPSCs sean vistas como una herramienta prometedora para los modelos intestinales en el futuro, los protocolos de diferenciaci\u00f3n tendr\u00edan que mejorar\u00bb, dice Elena Mart\u00ednez, investigadora principal del grupo. \u00abEso aumentar\u00eda a\u00fan m\u00e1s las similitudes entre estas c\u00e9lulas y los enterocitos, haci\u00e9ndolas a\u00fan m\u00e1s interesantes para su uso en modelos intestinales\u00bb.<\/p>\n

—<\/p>\n

Art\u00edculo de referencia:<\/em><\/strong> Maria Helena Macedo, Francisca Ara\u00fajo, \u00a0Elena Mart\u00ednez, Cristina Barrias, and Bruno Sarmento\u00a0(2018). iPSC-Derived Enterocyte-like Cells for Drug Absorption and Metabolism Studies<\/a>. Trends in Molecular Medicine<\/em>, 24, 8, 696-708<\/p>\n","protected":false},"excerpt":{"rendered":"

\"\"<\/a>El grupo del IBEC Biomimetic Systems for Cell Engineering<\/a> ha publicado un art\u00edculo de revisi\u00f3n sobre nuevas estrategias para estudiar la absorci\u00f3n de f\u00e1rmacos en el intestino en la revista de alto impacto Trends in Molecular Medicine<\/em>. <\/h3>\n

Junto con sus colaboradores en la Universidade do Porto, el grupo de Elena Mart\u00ednez examina el estado del arte de los modelos intestinales basados en c\u00e9lulas.<\/p>\n","protected":false},"author":4,"featured_media":60895,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[97],"tags":[825],"post_formats":[],"class_list":["post-60938","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-noticias-de-investigacion","tag-elena-martinez-es"],"yoast_head":"\nMejores modelos in vitro para estudiar el intestino humano - Institute for Bioengineering of Catalonia<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/ibecbarcelona.eu\/es\/mejores-modelos-in-vitro-para-estudiar-el-intestino-humano\/\" \/>\n<meta property=\"og:locale\" content=\"es_ES\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Mejores modelos in vitro para estudiar el intestino humano - Institute for Bioengineering of Catalonia\" \/>\n<meta property=\"og:description\" content=\"El grupo del IBEC Biomimetic Systems for Cell Engineering ha publicado un art\u00edculo de revisi\u00f3n sobre nuevas estrategias para estudiar la absorci\u00f3n de f\u00e1rmacos en el intestino en la revista de alto impacto Trends in Molecular Medicine. 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