{"id":8365,"date":"2014-07-25T07:06:52","date_gmt":"2014-07-25T07:06:52","guid":{"rendered":"http:\/\/ibecbarcelona.eu\/new\/profundizar-en-el-funcionamiento-interno-de-las-celulas\/"},"modified":"2015-02-05T10:05:15","modified_gmt":"2015-02-05T10:05:15","slug":"profundizar-en-el-funcionamiento-interno-de-las-celulas","status":"publish","type":"post","link":"https:\/\/ibecbarcelona.eu\/es\/profundizar-en-el-funcionamiento-interno-de-las-celulas\/","title":{"rendered":"Profundizar en el funcionamiento interno de las c\u00e9lulas"},"content":{"rendered":"<h6 class=\"caption\" style=\"width: 230px; float: left; padding: 0px 10px 10px 0px;\">Imagen: Una neurona hipocampal que se ha fotosensibilizado con los nuevos compuestos (izquierda), se puede estimular con un l\u00e1ser de impulsos de 900nm para producir una r\u00e1faga de potenciales de acci\u00f3n que se puede medir con la t\u00e9cnica de patch clamp (derecha)<\/h6>\n<p>El grupo de Nanosondas y Nanoconmutadores del IBEC y sus colaboradores en Espa\u00f1a y en EEUU han publicado un art\u00edculo cient\u00edfico en la revista JACS que plantea una nueva manera de utilizar las prote\u00ednas reguladas por la luz. Las prote\u00ednas &#8216;conmutables&#8217;, por control remoto, pueden activar o inhibir las funciones bioqu\u00edmicas espec\u00edficas en las c\u00e9lulas vivas, lo que permite a los investigadores configurar y probar una multitud de situaciones dentro de la c\u00e9lula &#8211; para activar o desactivar los procesos a un nivel mucho m\u00e1s profundo en el tejido y con mayor resoluci\u00f3n en 3D.<\/p>\n<p>\u00abLas biomol\u00e9culas fotoconmutables se utilizan para manipular con precisi\u00f3n los procesos bioqu\u00edmicos celulares pero, hasta ahora, nadie ha explorado un m\u00e9todo conocido como microscop\u00eda de excitaci\u00f3n de dos fotones &#8211; que ofrece una penetraci\u00f3n m\u00e1s profunda del tejido, con un volumen de excitaci\u00f3n m\u00e1s peque\u00f1o, y reduce la fototoxicidad y la fotodestrucci\u00f3n &#8211; en relaci\u00f3n con estos interruptores \u00ab, explica Pau Gorostiza, investigador principal del grupo de Nanosondas y Nanoconmutadores del IBEC y Profesor de Investigaci\u00f3n ICREA. \u00abAhora, los interruptores de prote\u00ednas existentes tambi\u00e9n se pueden utilizar con excitaci\u00f3n multi-fot\u00f3n con luz infrarroja cercana, proporcionando resoluci\u00f3n subcelular.\u00bb<\/p>\n<p>Los investigadores han ajustado la respuesta a la luz de prote\u00ednas fotoconmutables existentes, para adaptarlas a la tecnolog\u00eda de estimulaci\u00f3n de dos fotones.  De este modo han sido capaces de obtener el control a nivel unicelular y subcelular, sin alterar las propiedades funcionales normales de las prote\u00ednas, y con mayor precisi\u00f3n.<\/p>\n<p>Este descubrimiento constituye un avance importante en la optogen\u00e9tica y optofarmacolog\u00eda para controlar y visualizar la actividad de las neuronas con el fin de trazar un mapa de los circuitos neuronales.<\/p>\n<p>&#8212;<\/p>\n<p><strong>Art\u00edculo de referencia:<\/strong> Izquierdo-Serra M, Gasc\u00f3n-Moya M, Hirtz JJ, Pittolo S, Poskanzer KE, Ferrer E, Alib\u00e9s R, Busqu\u00e9 F, Yuste R, Hernando J, Gorostiza P. (2014). <a href=\"http:\/\/pubs.acs.org\/doi\/abs\/10.1021\/ja5026326\" target=\"_blank\">Two-Photon Neuronal and Astrocytic Stimulation with Azobenzene-Based Photoswitches<\/a>. <em>J Am Chem Soc<\/em>. 2014 Jun 18;136(24):8693-701<\/p>\n<p>\u00a0<\/p>\n<p>\u00a0<\/p>\n","protected":false},"excerpt":{"rendered":"<h3><img decoding=\"async\" class=\"left size-full wp-image-8360 lazyload\" data-src=\"https:\/\/ibecbarcelona.eu\/wp-content\/uploads\/2014\/07\/neuron_340x210.jpg\" border=\"0\" width=\"340\" height=\"210\" data-srcset=\"https:\/\/ibecbarcelona.eu\/wp-content\/uploads\/2014\/07\/neuron_340x210.jpg 340w, https:\/\/ibecbarcelona.eu\/wp-content\/uploads\/2014\/07\/neuron_340x210-300x185.jpg 300w, https:\/\/ibecbarcelona.eu\/wp-content\/uploads\/2014\/07\/neuron_340x210-242x150.jpg 242w, https:\/\/ibecbarcelona.eu\/wp-content\/uploads\/2014\/07\/neuron_340x210-150x92.jpg 150w\" data-sizes=\"(max-width: 340px) 100vw, 340px\" src=\"data:image\/svg+xml;base64,PHN2ZyB3aWR0aD0iMSIgaGVpZ2h0PSIxIiB4bWxucz0iaHR0cDovL3d3dy53My5vcmcvMjAwMC9zdmciPjwvc3ZnPg==\" style=\"--smush-placeholder-width: 340px; --smush-placeholder-aspect-ratio: 340\/210;\" \/>La investigaci\u00f3n llevada a cabo en el IBEC ha abierto camino hacia nuevas aplicaciones para controlar la activaci\u00f3n de las neuronas u otras partes funcionales de las c\u00e9lulas.<\/h3>\n<p>El sue\u00f1o de controlar con precisi\u00f3n y de forma remota todos los aspectos del funcionamiento interno celular en el tejido ofrece la promesa de descubrir los mecanismos moleculares de los procesos celulares complejos. Cosa que a su vez puede dar lugar a grandes avances en nuestra comprensi\u00f3n de lo que sucede cuando las cosas van mal &#8211; por ejemplo, c\u00f3mo y cu\u00e1ndo las enfermedades neurodegenerativas pueden desarrollarse.<\/p>\n","protected":false},"author":4,"featured_media":8361,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[97],"tags":[],"post_formats":[],"class_list":["post-8365","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-noticias-de-investigacion"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.4 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Profundizar en el funcionamiento interno de las c\u00e9lulas - Institute for Bioengineering of Catalonia<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/ibecbarcelona.eu\/es\/profundizar-en-el-funcionamiento-interno-de-las-celulas\/\" \/>\n<meta property=\"og:locale\" content=\"es_ES\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Profundizar en el funcionamiento interno de las c\u00e9lulas - Institute for Bioengineering of Catalonia\" \/>\n<meta property=\"og:description\" content=\"La investigaci\u00f3n llevada a cabo en el IBEC ha abierto camino hacia nuevas aplicaciones para controlar la activaci\u00f3n de las neuronas u otras partes funcionales de las c\u00e9lulas. El sue\u00f1o de controlar con precisi\u00f3n y de forma remota todos los aspectos del funcionamiento interno celular en el tejido ofrece la promesa de descubrir los mecanismos moleculares de los procesos celulares complejos. Cosa que a su vez puede dar lugar a grandes avances en nuestra comprensi\u00f3n de lo que sucede cuando las cosas van mal - por ejemplo, c\u00f3mo y cu\u00e1ndo las enfermedades neurodegenerativas pueden desarrollarse.\" \/>\n<meta property=\"og:url\" content=\"https:\/\/ibecbarcelona.eu\/es\/profundizar-en-el-funcionamiento-interno-de-las-celulas\/\" \/>\n<meta property=\"og:site_name\" content=\"Institute for Bioengineering of Catalonia\" \/>\n<meta property=\"article:published_time\" content=\"2014-07-25T07:06:52+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2015-02-05T10:05:15+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/ibecbarcelona.eu\/wp-content\/uploads\/2014\/07\/neuron_340x210.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"340\" \/>\n\t<meta property=\"og:image:height\" content=\"210\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"author\" content=\"\u00c0ngels L\u00f3pez\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Escrito por\" \/>\n\t<meta name=\"twitter:data1\" content=\"\u00c0ngels L\u00f3pez\" \/>\n\t<meta name=\"twitter:label2\" content=\"Tiempo de lectura\" \/>\n\t<meta name=\"twitter:data2\" content=\"2 minutos\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"Article\",\"@id\":\"https:\\\/\\\/ibecbarcelona.eu\\\/es\\\/profundizar-en-el-funcionamiento-interno-de-las-celulas\\\/#article\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/ibecbarcelona.eu\\\/es\\\/profundizar-en-el-funcionamiento-interno-de-las-celulas\\\/\"},\"author\":{\"name\":\"\u00c0ngels L\u00f3pez\",\"@id\":\"https:\\\/\\\/ibecbarcelona.eu\\\/#\\\/schema\\\/person\\\/c212c6b589546130be67986b9290e2b8\"},\"headline\":\"Profundizar en el funcionamiento interno de las c\u00e9lulas\",\"datePublished\":\"2014-07-25T07:06:52+00:00\",\"dateModified\":\"2015-02-05T10:05:15+00:00\",\"mainEntityOfPage\":{\"@id\":\"https:\\\/\\\/ibecbarcelona.eu\\\/es\\\/profundizar-en-el-funcionamiento-interno-de-las-celulas\\\/\"},\"wordCount\":408,\"publisher\":{\"@id\":\"https:\\\/\\\/ibecbarcelona.eu\\\/#organization\"},\"image\":{\"@id\":\"https:\\\/\\\/ibecbarcelona.eu\\\/es\\\/profundizar-en-el-funcionamiento-interno-de-las-celulas\\\/#primaryimage\"},\"thumbnailUrl\":\"https:\\\/\\\/ibecbarcelona.eu\\\/wp-content\\\/uploads\\\/2014\\\/07\\\/neuron_340x210.jpg\",\"articleSection\":[\"Noticias de investigaci\u00f3n\"],\"inLanguage\":\"es\"},{\"@type\":\"WebPage\",\"@id\":\"https:\\\/\\\/ibecbarcelona.eu\\\/es\\\/profundizar-en-el-funcionamiento-interno-de-las-celulas\\\/\",\"url\":\"https:\\\/\\\/ibecbarcelona.eu\\\/es\\\/profundizar-en-el-funcionamiento-interno-de-las-celulas\\\/\",\"name\":\"Profundizar en el funcionamiento interno de las c\u00e9lulas - 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