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Publications

by Keyword: Nmr j-spectroscopy

Eills, James, Picazo-Frutos, Roman, Bondar, Oksana, Cavallari, Eleonora, Carrera, Carla, Barker, Sylwia J, Utz, Marcel, Herrero-Gomez, Alba, Marco-Rius, Irene, Tayler, Michael C D, Aime, Silvio, Reineri, Francesca, Budker, Dmitry, Blanchard, John W, (2023). Enzymatic Reactions Observed with Zero- and Low-Field Nuclear Magnetic Resonance Analytical Chemistry 95, 17997-18005

We demonstrate that enzyme-catalyzed reactions can be observed in zero- and low-field NMR experiments by combining recent advances in parahydrogen-based hyperpolarization methods with state-of-the-art magnetometry. Specifically, we investigated two model biological processes: the conversion of fumarate into malate, which is used in vivo as a marker of cell necrosis, and the conversion of pyruvate into lactate, which is the most widely studied metabolic process in hyperpolarization-enhanced imaging. In addition to this, we constructed a microfluidic zero-field NMR setup to perform experiments on microliter-scale samples of [1-C-13]-fumarate in a lab-on-a-chip device. Zero- to ultralow-field (ZULF) NMR has two key advantages over high-field NMR: the signals can pass through conductive materials (e.g., metals), and line broadening from sample heterogeneity is negligible. To date, the use of ZULF NMR for process monitoring has been limited to studying hydrogenation reactions. In this work, we demonstrate this emerging analytical technique for more general reaction monitoring and compare zero- vs low-field detection.

JTD Keywords: Nmr j-spectroscopy