PhD Thesis Defence: Nimesh Ramesh Chahare

Biography:

Nimesh Ramesh Chahare earned his bachelor’s degree in Mechanical Engineering from the National Institute of Technology, Nagpur in 2014. He further pursued a master’s degree at the Indian Institute of Science, Bengaluru, completing it in 2016. In 2017, he began his doctoral work in Barcelona, under the guidance of Prof. Marino Arroyo at Universitat Politècnica de Catalunya, and Prof. Xavier Trepat at the Institute of Bioengineering of Catalonia. Currently, he is a PhD candidate in applied mathematics, specializing in the study of the mechanics of epithelial tissue under controlled pressure.

Abstract:

Epithelial sheets form specialized 3D structures suited to their physiological roles, such as branched alveoli in the lungs, tubes in the kidney, and villi in the intestine. To generate and maintain these structures, epithelia must undergo complex 3D deformations across length and time scales. How epithelial shape arises from active stresses, viscoelasticity, and luminal pressure remains poorly understood. To address this question, we developed a microfluidic chip and a computational framework to engineer 3D epithelial tissues with controlled shape and pressure. In the setup, an epithelial monolayer is grown on a porous surface with circular low adhesion zones. On applying hydrostatic pressure, the monolayer delaminates into a spherical cap from the circular zone. This simple shape allows us to calculate epithelial tension using Laplace’s law. Through this approach, we subject the monolayer to a range of lumen pressures at different rates and hence probe the relation between strain and tension in different regimes while computationally tracking actin dynamics and their mechanical effect at the tissue scale. Slow pressure changes relative to the actin dynamics allow the tissue to accommodate large strain variations. However, under sudden pressure reductions, the tissue develops buckling patterns and folds with different degrees of symmetry-breaking to store excess tissue area. These insights allow us to pattern epithelial folds through rationally directed buckling. Our study establishes a new approach for engineering epithelial morphogenetic events.

Reading date: 20/06/2023
Reading time: 15:00

Reading place: Facultat de Matemàtiques i Estadística-Sala d’Actes FME, Edifici U, Campus Sud
Thesis director: ARROYO BALAGUER, MARINO and TREPAT GUIXER, XAVIER
Committee:
PRESIDENT: TRIVEDI, VIKAS
SECRETARI: MUÑOZ ROMERO, JOSE JAVIER
VOCAL: SUZANNE, MAGALI