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by Keyword: Antibody delivery
Intrarakasem, N, Kaewkarn, S, Proykhunthod, P, Songjaeng, A, Avirutnun, P, Prommool, T, Puttikhunt, C, Makeudom, A, Morchang, A, Tian, XH, Battaglia, G, Patikarnmonthon, N, Kraivong, R, (2026). Intracellular delivery of anti-dengue envelope monoclonal antibodies via PEG-PLGA nanoparticles suppresses viral replication and attenuates hepatocyte apoptosis JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY 118, 108086 
Dengue virus (DENV) infection remains a major global health threat, with no specific antiviral treatment currently approved. Monoclonal antibody (mAb) therapy represents a promising strategy for viral inhibition; however, conventional antibodies are largely restricted to extracellular compartments and lack access to intracellular viral replication sites. In this study, we encapsulated a cross-reactive mAb targeting the DENV envelope protein (m513) into poly(ethylene glycol)-block-poly(lactide-co-glycolide) (PEG-PLGA) nanoparticles to facilitate intracellular delivery. When applied to immortalized hepatocyte-like cells (imHCs), the formulation demonstrated efficient cellular uptake, low cytotoxicity, and significantly reduced intracellular viral RNA and protein levels. The resulting formulation consisted of mAb-loaded PEG-PLGA nanoparticles (similar to 100 nm in diameter) with spherical morphology and an encapsulation efficiency of approximately 50%. Furthermore, nanoparticle treatment significantly reduced hepatocyte apoptosis in infected cells. Collectively, these findings demonstrate that nanoparticle-mediated intracellular antibody delivery can overcome a key limitation of conventional antibody therapy and represents a mechanistically distinct antiviral strategy for DENV and other intracellular viral infections.
JTD Keywords: Antibody delivery, Dengue virus, Drug-delivery, Endocytosis, In-vitro, Monoclonal antibody, Peg-plga nanoparticle, Protein
