IBEC Seminar: Aurélien Bancaud

µLAS technology for DNA processing: setting up elementary functions (concentration, separation, purification, identification) and application in oncology and targeted sequencing

We recently developed the µLAS technology for nucleic acids processing. Its operating principle relies on the monitoring of DNA transport in a viscoelastic liquid under the combined action of hydrodynamic and electrophoretic forces (1). DNA molecules are dragged toward the walls of microchannels by a transverse force proportional to their contour length. Because the hydrodynamic decreases near the wall, DNA molecules are sorted according to their molecular weight. Furthermore, by tailoring the geometry of a channel with a constriction, we can tune the amplitude of transverse forces and stop molecules to design a concentrator that achieves enrichment rates of 100 to 1000 fold per minute.

We will derive a quantitative model of DNA transport in µLAS that relies on 1 fitting parameter and perform rational optimizations of the technology. We will then exploit µLAS for sizing cell-free circulating DNA (cfDNA) in the blood (2), and demonstrate that cfDNA profiling is a promissing biomarker for the follow-up of cancer patients. Finally, we will present the principle of a DNA size-selective valve for the purification and sequencing of target genomic regions by combining µLAS with Cas9 endonuclease.

(1) Ranchon et al., Lab Chip (2016)
(2) Andriamanampisoa et al., Anal Chem (2018)

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