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Pluripotency for organ regeneration

About

The generation of induced pluripotent stem cells (iPSCs), especially the generation of patient-derived pluripotent stem cells suitable for disease modelling in vitro, opens the door for the potential translation of stem-cell related studies into the clinic. 

Successful replacement, or augmentation, of the function of damaged cells by patient derived differentiated stem cells would provide a novel cell-based therapy for diseases. Since iPSCs resemble human embryonic stem cells (hESCs) in their ability to generate cells of three germ layers, patient-specific iPSCs offer definitive solutions for the ethical and histo-incompatibility issues related to hESCs. Indeed human iPSC (hiPSC)-based autologous transplantation is heralded as the future of regenerative medicine.

One of our aims is to generate and correct disease-specific hiPSCs for disease modelling and drug screening. The combination of gene-editing based methodologies together with the development of novel protocols for cell differentiation into relevant tissues/organs, provides a unique scenario for modelling disease progression, and the identification of molecular and cellular mechanisms leading to organ regeneration (Figure 1). In this regard we are particularly interested in generation of transgene-free and disease free patient derived hiPSCs for disease modelling and the discovery of novel therapeutic targets.  

We believe that the recovery of tissue function should not be restricted to the development of cell replacement therapies. In this regard, in our laboratory we take advantage of organisms that possess the ability to regenerate such as zebrafish, in order to understand which molecular and cellular pathways lead to organ regeneration.

Surprisingly, studies in neonatal mice have demonstrated that soon after birth this organism posses the capability to regenerate its heart. Taking advantage of such preliminary observations we are translating such analysis in order to understand if the mammalian neonatal kidney still posses the capability to regenerate, and more importantly, if we are able to dissect the epigenetic and cellular mechanisms leading to those responses. 

Lastly, and in an effort to fully develop in vitro and ex vivo platforms for organ regeneration, in our lab we are focused in the development of reporter cell lines for different transcription factors essential for tissue-specific commitment and differentiation (i.e: renal and cardiac lineages). The possibility to combine pluripotent stem cell lines together with decellularized matrices, functionalized biomaterials and ex vivo organoids offers and unprecedented opportunity for the immediate generation of patient-specific in vitro and ex vivo platforms for disease modelling and organ regeneration (Figure 2). 

Staff

Projects

NATIONAL PROJECTSFINANCERPI
Bioengeniería para mejorar la salud mediante de organoides y bioimpresión 3D (2021-2023)MINECO – Plataformas ISCIII de apoyo a la I+D+I en Biomedicina y Ciencias de la SaludNúria Montserrat
CARDIOPRINT Biofabricación avanzada multifunción en 3D para la generación de tejido cardiaco terapéutico a escala humana diseñado por ordenador (2021-2024)MICIU, Proyectos de I+D+i en líneas estratégicasNúria Montserrat
CAKUTORG Desarrollando nuevas estrategias para entender y tratar las anomalías congénitas del riñón y del tracto urinario mediante organoides (2021-2024)MICIU, Retos investigación: Proyectos I+D Núria Montserrat
Identifying SARS-CoV-2- host cell interactions exploiting CRISPR/Cas9 engineered human organoids: through the development of specific therapies against COVID19 (2020-2022)FBBVA Núria Montserrat
CHONDREG · Identification of the epigenetic mechanisms preventing chondrocyte de-differentiation: generation of novel therapeutic strategies for the treatment of cartilage chronic osteochondral lesionsCIBERNuria Montserrat
Infarto de miocardio en jóvenes. Factores epigeneticos y nuevos marcadores de riesgo cardiovascular. Efecto de la modulación de la expresión de microRNAs y long-non coding RNAsISCIII(Collaborator)
INTERNATIONAL FUNDED PROJECTSFINANCERPI
ENGIORG Engineering kidney organoids to study the interplay between Tissue Mechanics and Metabolism: from development to disease (2021-2026)European Commission Núria Montserrat
ECaBox ECaBox «Eyes in a Care Box»: Regenerating human retina from resuscitated cadaveric eyes (2021-2025)European Commission, FET OPENNúria Montserrat
Engineering functional human kidneys and urinary tracts (2021-2024)Wellcome Leap Solicitation for Humans Organs, Physiology and Engineering (HOPE) Núria Montserrat
BRAV3. Computational biomechanics and bioengineering 3D printing to develop a personalized regenerative biological ventricular assist device to provide lasting functional support to damaged hearts (2020-2024)European CommissionNúria Montserrat
MAD-CoV 2 · Modern approaches for developing antivirals against SARS-CoV 2 (2020-2024)European CommissionNúria Montserrat
R2U-Tox-Assay · Ready-to-use Toxicity Screening Assay based on iPS-Technologies (2020-2022)EIT HealthNúria Montserrat
PRIVATELY FUNDED PROJECTSFINANCERPI
SYSTORG Exploiting organoid model systems to explore systemic conditions worsening COVID19: merging cellular and genetic engineering (2021-2024)Fundació La Marató de TV3, TV3-Projectes de recerca La Marató TV3 Núria Montserrat
Identificació de noves dianes terapèutiques i biomarcadors de progressió del càncer de ronyó a través de models organoides i xenoempelts genèticament dissenyats per CRISPR (2020-2023)Fundació La Marató de TV3, TV3-Projectes de recerca La Marató TV3 Núria Montserrat
REPIRE · Regenerating photoreceptors in human retinal organoids to establish a treatment for Retinitis Pigmentosa (2018-2021)Fundación Bancaria «La Caixa»Núria Montserrat
FUNDRAISING PROJECTSFINANCERPI
Programa Faster Future 2020: COVID-19 (2021)FundraisingNúria Montserrat
FINISHED PROJECTSFINANCERPI
Identification of Kidney Cancer progression targets and biomarkers through CRISPR-engineered organoids and xenograft mouse models (2019-2020)Fundació La Marató de TV3Núria Montserrat
Generation of Isogenic Models of Clear Cell Renal Cell Carcinoma (ccRCC) using CRISPR-engineered Kidney Organoids, for the identification of diagnostic biomarkers (2017-2021)Fundación AECCNúria Montserrat
EPIORG · Cómo modelar la Nefropatía Diabética: restableciendo el epigenoma en organoides renales diabéticos inducidos (2018-2020)MINECO, Retos investigación: Proyectos I+DNúria Montserrat
MECHANORG · Como integrar señales mecánicas y metabólicas en organoides renales para el modelado de patologías humanas (2019-2020)MINECO, Acciones Dinamización Europa InvestigaciónNúria Montserrat
Modelling Diabetic Nephropathy targeting DNA methylation: engineering the epigenome in kidney (2019-2020)EFSD European Foundation for the Study of DiabetesNúria Montserrat
REGMAMKID · How to regenerate the mammalian kidney (2015-2021)European Commission, ERC-StGNúria Montserrat
REPROMICRO · Reprogramacion y regeneracion tisular a partir de microvesiculas derivadas de celulas madre de pluripotencia inducida (2017-2019)Ministerio de Economía y Competitividad, Explora Ciencia Nuria Montserrat
Desarrollo de nuevas estrategias para el tratamiento de la enfermedad renal (2015-2017)MINECONuria Montserrat
TRATENFREN · Desarrollo de nuevas estrategias para el tratamiento de la enfermedad renal (2015-2017)MINECO, Retos investigación: Proyectos I+DNuria Montserrat
Regenerative medicine for Fanconi anemia: generation of disease-free patient-specific iPS (2013-2016)Fundació La Marató de TV3Nuria Montserrat
ACE2-ORG · Development of a human cellular plaform unveilling Angiotensin-converting enzyme 2 (ACE2) – sars-CoV-2 interactions (2020-2021)ISCIIINúria Montserrat
Red TERCEL · Red de Terapia Celular (2017-2021)MINECO, ISCIII(Collaborator)
EPIORGABOLISM Diabetic nephropathy modelling in hESC-derived 3D (2019-2021)European Commission, MARIE CURIE – IFCarmen Hurtado

Publications



Equipment

  • Real Time QuantStudio 5
  • SimpliAmp thermocycler
  • Eppendorf 5415D centrifuge
  • Allegra X-15 R centrifuge
  • Gyrozen 1248 centrifuge
  • BioUltra 6 Telstar culture Hood 2x
  • AH-100 Telstar primary culture Hood
  • Binder CB 60 incubators 2x
  • Controltecnica ASTEC SCA 165 incubator
  • Controltecnica ZC 180 incubator
  • Bioruptor Pico sonicator
  • Thermomixer C thermal block
  • Leica DMS1000 and DMIL Led microscopes
  • Leica DMi1 microscope
  • Leica MZ 10F magnifying glass
  • Safe Imager 2.0 transilluminator

Collaborations

  • Juan Carlos Izpisua Belmonte
    Salk Institute for Biological Studies
  • Dr. Josep Maria Campistol Plana
    Experimental Laboratory of Nephrology and Transplantation, Hospital Clínic, Barcelona
  • Peter Hohestein
    The Roslin Institute, University of Edinburgh
  • Dr. Pere Gascón Vilaplana
    Head of Oncology Service/Molecular and Translational Oncology Laboratory, IDIBAPS
  • Gloria Calderon
    Embryotools SL
  • Pura Muñoz Cánovas
    Departament de Ciències Experimentals i de la Salut, Universitat Pompeu Fabra
  • Dr. Pedro Guillén
    Director Clínica Cemtro, Madrid
  • Dr. Francisco Fernández Avilés
    Head of Cardiology Service, Hospital General Universitario Gregorio Marañón, Madrid
  • Dr María Eugenia Fernández
    Unit of Cell Production, Hospital Gregorio Marañón, Madrid
  • Joaquin Gutiérrez Fruitós
    University of Barcelona
  • Dr. Pere Roca-Cusachs
    IBEC
  • Dr. Elena Martínez
    IBEC
  • Dr. Cristina Eguizabal  Argaiz
    Centro Vasco de Transfusion y Tejidos Humanos (CVTTH), Bizkaia
  • Dr. Antonio Alcaraz
    Head of Urology, Hospital Clínic, Barcelona
  • Dr. Oriol Casanovas
    Head of Tumour Angiogenesis Group, IDIBELL

News

Núria Montserrat, profesora de investigación ICREA e investigadora principal en el Instituto de Bioingeniería de Cataluña (IBEC), ha sido galardonada con el Premio Constantes y Vitales al Joven Talento en … Read more

Núria Montserrat gana el premio Constantes y Vitales por su investigación pionera en el campo de los organoides 

Núria Montserrat, profesora de investigación ICREA e investigadora principal en el Instituto de Bioingeniería de Cataluña (IBEC), ha sido galardonada con el Premio Constantes y Vitales al Joven Talento en … Read more

Yesterday, the report «Lessons and challenges after two years of pandemic» was presented at the Carlos III Health Institute in Madrid, the first in a series of studies on trends in Spanish research that the Spanish Foundation for Science and Technology (FECYT) will carry out each year and which will analyze current scientific issues.

“Lecciones y retos tras dos años de pandemia” un informe de la FECYT con colaboración del IBEC

Yesterday, the report «Lessons and challenges after two years of pandemic» was presented at the Carlos III Health Institute in Madrid, the first in a series of studies on trends in Spanish research that the Spanish Foundation for Science and Technology (FECYT) will carry out each year and which will analyze current scientific issues.

En una colaboración internacional, investigadores liderados por Nuria Montserrat, Profesora de Investigación ICREA en el IBEC, han generado minirriñones humanos que simulan el riñón de una persona con diabetes en las etapas iniciales de la enfermedad. Estos minirriñones diabéticos abren las puertas a estudiar, entre otros, la relación entre la diabetes y la COVID-19.

Minirriñones generados en el laboratorio ayudan a entender la relación entre la enfermedad de la diabetes y la COVID-19

En una colaboración internacional, investigadores liderados por Nuria Montserrat, Profesora de Investigación ICREA en el IBEC, han generado minirriñones humanos que simulan el riñón de una persona con diabetes en las etapas iniciales de la enfermedad. Estos minirriñones diabéticos abren las puertas a estudiar, entre otros, la relación entre la diabetes y la COVID-19.

Núria Montserrat, investigadora principal del Instituto de Bioingeniería de Cataluña (IBEC), aparece en el País semanal por su trabajo en el desarrollo de organoides a través de células madre pluripotentes que, en un futuro, podrían utilizarse para los transplantes.

Núria Montserrat y ciencia en el País semanal

Núria Montserrat, investigadora principal del Instituto de Bioingeniería de Cataluña (IBEC), aparece en el País semanal por su trabajo en el desarrollo de organoides a través de células madre pluripotentes que, en un futuro, podrían utilizarse para los transplantes.

Los medios han publicado el descubrimiento realizado por científicos del Centro de Regulación Genómica (CRG) y financiado principalmente por un Proyecto de CaixaResearch de Investigación en Salud de la Fundació «la Caixa» otorgado a Pia Cosma (CRG), Nuria Montserrat (IBEC) y Justin Christopher D’Antin (Barraquer).

Avance hacia la regeneración de la retina humana

Los medios han publicado el descubrimiento realizado por científicos del Centro de Regulación Genómica (CRG) y financiado principalmente por un Proyecto de CaixaResearch de Investigación en Salud de la Fundació «la Caixa» otorgado a Pia Cosma (CRG), Nuria Montserrat (IBEC) y Justin Christopher D’Antin (Barraquer).

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Jobs

Introduction to the vacant position: The Pluripotency for organ regeneration Group is looking for a Postdoc to develop his/her project on the fabrication of microfluidic devices for tissue engineered organ-on-a-chip … Read more

Senior Laboratory technician at the Pluripotency for organ regeneration Research Group (Mad-CoV2)

Introduction to the vacant position: The Pluripotency for organ regeneration Group is looking for a Postdoc to develop his/her project on the fabrication of microfluidic devices for tissue engineered organ-on-a-chip … Read more

Introduction to the vacant position: The Pluripotency for organ regeneration Group at the Institute for Bioengineering of Catalonia (IBEC) led by Prof. Nuria Montserrat is looking for a laboratory technician … Read more

Laboratory technician at the Pluripotency for organ regeneration Research Group

Introduction to the vacant position: The Pluripotency for organ regeneration Group at the Institute for Bioengineering of Catalonia (IBEC) led by Prof. Nuria Montserrat is looking for a laboratory technician … Read more

Introduction to the vacant position: The Pluripotency for organ regeneration Group is looking for a Postdoc to develop his/her project on the fabrication of microfluidic devices for tissue engineered organ-on-a-chip … Read more

Postdoctoral at the Pluripotency for organ regeneration Research Group (TERAV Project)

Introduction to the vacant position: The Pluripotency for organ regeneration Group is looking for a Postdoc to develop his/her project on the fabrication of microfluidic devices for tissue engineered organ-on-a-chip … Read more

Application Deadline: 23/09/2022Ref: PD-NM The Pluripotency for organ regeneration group at the Institute for Bioengineering of Catalonia (IBEC) is looking for a Postdoctoral researcher to develop his/her project on the fabrication of microfluidic devices for tissue engineered organ-on-a-chip cultures during health and upon SARS-CoV-2 infection. The contract will be within the framework of “Modern approaches for developing antivirals against SARS-CoV 2” from the Innovative Medicines Initiative 2 Joint Undertaking (JU) under grant agreement no. 101005026 .

Postdoctoral Researcher at the Pluripotency for organ regeneration Research Group

Application Deadline: 23/09/2022Ref: PD-NM The Pluripotency for organ regeneration group at the Institute for Bioengineering of Catalonia (IBEC) is looking for a Postdoctoral researcher to develop his/her project on the fabrication of microfluidic devices for tissue engineered organ-on-a-chip cultures during health and upon SARS-CoV-2 infection. The contract will be within the framework of “Modern approaches for developing antivirals against SARS-CoV 2” from the Innovative Medicines Initiative 2 Joint Undertaking (JU) under grant agreement no. 101005026 .