Here, we present a flexible and low-cost inkjet printed electrochemical sensor for enzyme-free glucose analysis. Versatility, short fabrication time and low cost make inkjet printing a valuable alternative to traditional sensor manufacturing techniques. We fabricated electro-chemical glucose sensors by inkjet printing electrodes on a flexible polyethylene terephthalate substrate. CuO microparticles were used to modify our electrodes, leading to a sensitive, stable and cost-effective platform for non-enzymatic detection of glucose. Selectivity, reproducibility, and life-time provided by the CuO functionalization demonstrated that these sensors are reliable tools for personalized diagnostics and self-assessment of an individual's health. The detection of glucose at concentrations matching that of tear fluid allows us to envisage applications in ocular diagnostics, where painless and non-invasive monitoring of diabetes can be achieved by analyzing glucose contained in tears.