Staff member


Jessica Sierra Agudelo

PhD Student
Nanobioengineering
jsierra@ibecbarcelona.eu
+34 37185
Staff member publications

Sierra, J., Marrugo-Ramírez, J., Rodriguez-Trujillo, R., Mir, M., Samitier, J., (2020). Sensor-integrated microfluidic approaches for liquid biopsies applications in early detection of cancer Sensors 20, (5), 1317

Cancer represents one of the conditions with the most causes of death worldwide. Common methods for its diagnosis are based on tissue biopsies—the extraction of tissue from the primary tumor, which is used for its histological analysis. However, this technique represents a risk for the patient, along with being expensive and time-consuming and so it cannot be frequently used to follow the progress of the disease. Liquid biopsy is a new cancer diagnostic alternative, which allows the analysis of the molecular information of the solid tumors via a body fluid draw. This fluid-based diagnostic method displays relevant advantages, including its minimal invasiveness, lower risk, use as often as required, it can be analyzed with the use of microfluidic-based platforms with low consumption of reagent, and it does not require specialized personnel and expensive equipment for the diagnosis. In recent years, the integration of sensors in microfluidics lab-on-a-chip devices was performed for liquid biopsies applications, granting significant advantages in the separation and detection of circulating tumor nucleic acids (ctNAs), circulating tumor cells (CTCs) and exosomes. The improvements in isolation and detection technologies offer increasingly sensitive and selective equipment’s, and the integration in microfluidic devices provides a better characterization and analysis of these biomarkers. These fully integrated systems will facilitate the generation of fully automatized platforms at low-cost for compact cancer diagnosis systems at an early stage and for the prediction and prognosis of cancer treatment through the biomarkers for personalized tumor analysis.

Keywords: Cancer, Circulant tumor cells (CTC), Circulant tumor DNA (ctDNA), Exosomes, Liquid biopsy, Microfluidic, Sensors


Basas, J., Rojo, E., Gomis, X., Sierra, J.M., Torrents, E., Almirante, B., Gavaldà, J., (2015). Actividad de anidulafungia vs. anfotericina b liposomal frente a C. Parapsilopsis creciendo en biopelículas en distintos materiales Enfermedades Infecciosas y Microbiología Clínica XIX Congreso de la Sociedad Española de Enfermedades Infecciosas y Microbiología Clínica (SEIMC) , Elsevier (Sevilla, Spain) 33, 37

La infección del catéter venoso central está estrechamente relacionada con la capacidad de los microorganismos para producir biopelículas. Existen metodologías distintas para el estudio in vitro de la sensibilidad antibiótica de microorganismos creciendo en biopelículas; con placas de microtitulación (poliestireno) y con diferentes materiales (discos silicona, placas titanio...). Se ha descrito que las especies de Candida crecen con morfologías diferentes dependiendo del sustrato donde se implantan. Por tanto, la elección del material donde crecen las biopelículas podría tener su importancia en los estudios de sensibilidad in vitro. Previamente, en un modelo experimental de infección de catéter por C. parapsilopsis observamos que el sellado con anidulafungina (And) era más eficaz que con anfotericina B liposomal (LAmB). Teniendo en cuenta estas consideraciones el mejor sustrato para valorar la eficacia in vitro sería en discos de silicona (utilizado para la fabricación de catéteres) y no en placas de microtitulación (técnica estándar).