by Keyword: Microbiología

Garreta E, Moya-Rull D, Stanifer ML, Monteil V, Prado P, Marco A, Tarantino C, Gallo M, Jonsson G, Hagelkruys A, Mirazimi A, Boulant S, Penninger JM, Montserrat N., (2022). Protocol for SARS-CoV-2 infection of kidney organoids derived from human pluripotent stem cells Star Protocols 3, 101872

This protocol presents the use of SARS-CoV-2 isolates to infect human kidney organoids, enabling exploration of the impact of SARS-CoV-2 infection in a human multicellular in vitro system. We detail steps to generate kidney organoids from human pluripotent stem cells (hPSCs) and emulate a diabetic milieu via organoids exposure to diabetogenic-like cell culture conditions. We further describe preparation and titration steps of SARS-CoV-2 virus stocks, their subsequent use to infect the kidney organoids, and assessment of the infection via immunofluorescence.

JTD Keywords: cell culture, cell differentiation, microbiology, microscopy, organoids, Cell culture, Microbiología, Microscopy, Stem cells