by Keyword: Microbiología
Garreta, E, Moya-Rull, D, Stanifer, ML, Monteil, V, Prado, P, Marco, A, Tarantino, C, Gallo, M, Jonsson, G, Hagelkruys, A, Mirazimi, A, Boulant, S, Penninger, JM, Montserrat, N, (2022). Protocol for SARS-CoV-2 infection of kidney organoids derived from human pluripotent stem cells Star Protocols 3, 101872 
This protocol presents the use of SARS-CoV-2 isolates to infect human kidney organoids, enabling exploration of the impact of SARS-CoV-2 infection in a human multicellular in vitro system. We detail steps to generate kidney organoids from human pluripotent stem cells (hPSCs) and emulate a diabetic milieu via organoids exposure to diabetogenic-like cell culture conditions. We further describe preparation and titration steps of SARS-CoV-2 virus stocks, their subsequent use to infect the kidney organoids, and assessment of the infection via immunofluorescence.
JTD Keywords: cell culture, cell differentiation, microbiology, microscopy, organoids, Cell culture, Cell differentiation, Covid-19, Humans, Kidney, Microbiología, Microbiology, Microscopy, Organoids, Pluripotent stem cells, Sars-cov-2, Stem cells
