by Keyword: Tyrosine phosphorylation
Lagunas, Anna, Gomila, Alexandre M J, Gomila, Alexandre M J, Nin Hill, Alba, Guerra-Castellano, Alejandra, Perez-Mejias, Gonzalo, Samitier, Josep, Rovira, Carme, De la Rosa, Miguel A, De la Rosa, Miguel A, Diaz-Moreno, Irene, Gorostiza, Pau, (2025). Long-Distance Charge Transport between Cytochrome c and Complex III is Mediated by Protons and Reactive Oxygen Species Small 21, e01286 
Electron transfer (ET) between redox proteins is an essential process in the respiratory and photosynthetic transport chains. While intra-protein ET is well characterized, the experimental methods to investigate inter-protein ET are limited by the presence of the solvent and by the transient nature of the protein-protein interaction and ET event, which are averaged in protein ensembles. Wiring precisely oriented redox protein partners to the nanoscale electrodes of an electrochemical scanning tunneling microscope allows recording the time- and distance-dependence of the current flowing between them. These methods have revealed that the current flowing between individual protein pairs extends beyond tunneling distances and that it is electrochemically gated. However, the corresponding mechanism and the identity of the charge carriers in aqueous solution remain to be elucidated. To determine the species involved in long-distance charge transport between the redox partner proteins Cc and Cc 1 of the respiratory chain, recordings are performed as a function of pH, in heavy water solutions, and in degassed solutions. It is observed that the spatial span and electrochemical gating of long-distance currents are reduced at high pH, in heavy water, and at low oxygen concentration, showing that the currents are assisted by superoxide anions and by protons.
JTD Keywords: Catalysis, Coupled electron-transfer, Dynamics, Electrochemical stm, Gouy-chapman conduit, Grotthuss (grothuss) proton hopping conduction, Interface, Kinetic isotope effect kie, Mechanism, Mitochondria, Pathways, Ph, Proteins, Proton coupled electron transfer pcet, Reactive oxygen species ros, Reductase, Superoxide radical anion sox, Tyrosine phosphorylation
Gomila, AMJ, Pérez-Mejías, G, Nin-Hill, A, Guerra-Castellano, A, Casas-Ferrer, L, Ortiz-Tescari, S, Díaz-Quintana, A, Samitier, J, Rovira, C, De la Rosa, MA, Díaz-Moreno, I, Gorostiza, P, Giannotti, M, Lagunas, A, (2022). Phosphorylation disrupts long-distance electron transport in cytochrome c Nature Communications 13, 7100
It has been recently shown that electron transfer between mitochondrial cytochrome c and the cytochrome c1 subunit of the cytochrome bc1 can proceed at long-distance through the aqueous solution. Cytochrome c is thought to adjust its activity by changing the affinity for its partners via Tyr48 phosphorylation, but it is unknown how it impacts the nanoscopic environment, interaction forces, and long-range electron transfer. Here, we constrain the orientation and separation between cytochrome c1 and cytochrome c or the phosphomimetic Y48pCMF cytochrome c, and deploy an array of single-molecule, bulk, and computational methods to investigate the molecular mechanism of electron transfer regulation by cytochrome c phosphorylation. We demonstrate that phosphorylation impairs long-range electron transfer, shortens the long-distance charge conduit between the partners, strengthens their interaction, and departs it from equilibrium. These results unveil a nanoscopic view of the interaction between redox protein partners in electron transport chains and its mechanisms of regulation.© 2022. The Author(s).
JTD Keywords: apoptosis, binding, cardiolipin, complex, dynamics, force, respiration, structural basis, tyrosine phosphorylation, Histone chaperone activity
