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Publications

by Keyword: gtpase

Phuyal, Santosh, Djaerff, Elena, Le Roux, Anabel‐Lise, Baker, Martin J, Fankhauser, Daniela, Mahdizadeh, Sayyed Jalil, Reiterer, Veronika, Parizadeh, Amirabbas, Felder, Edward, Kahlhofer, Jennifer C, Teis, David, Kazanietz, Marcelo G, Geley, Stephan, Eriksson, Leif, Roca‐Cusachs, Pere, Farhan, Hesso, (2022). Mechanical strain stimulates COPII-dependent secretory trafficking via Rac1 Embo Journal 41, e110596

Leite, DM, Seifi, M, Ruiz-Perez, L, Nguemo, F, Plomann, M, Swinny, JD, Battaglia, G, (2022). Syndapin-2 mediated transcytosis of amyloid-beta across the blood brain barrier Brain Commun 4, fcac039

A deficient transport of amyloid-beta across the blood-brain barrier, and its diminished clearance from the brain, contribute to neurodegenerative and vascular pathologies, such as Alzheimer's disease and cerebral amyloid angiopathy, respectively. At the blood-brain barrier, amyloid-beta efflux transport is associated with the low-density lipoprotein receptor-related protein 1. However, the precise mechanisms governing amyloid-beta transport across the blood-brain barrier, in health and disease, remain to be fully understood. Recent evidence indicates that the low-density lipoprotein receptor-related protein 1 transcytosis occurs through a tuhulation-mediated mechanism stabilized by syndapin-2. Here, we show that syndapin-2 is associated with amyloid-beta clearance via low-density lipoprotein receptor-related protein 1 across the blood-brain barrier. We further demonstrate that risk factors for Alzheimer's disease, amyloid-beta expression and ageing, are associated with a decline in the native expression of syndapin-2 within the brain endothelium. Our data reveals that syndapin-2-mediated pathway, and its balance with the endosomal sorting, are important for amyloid-beta clearance proposing a measure to evaluate Alzheimer's disease and ageing, as well as a target for counteracting amyloid-beta build-up. Moreover, we provide evidence for the impact of the avidity of amyloid-beta assemblies in their trafficking across the brain endothelium and in low-density lipoprotein receptor-related protein 1 expression levels, which may affect the overall clearance of amyloid-beta across the blood-brain barrier.

JTD Keywords: Alzheimer's disease, Alzheimers-disease, Amyloid-beta, Apolipoprotein-j, Blood-brain barrier, Clearance, Expression, Membrane invagination, Peptide, Protein, Rab gtpases, Receptor, Syndapin-2, Transport, Tubular transcytosis


De Matteis V, Rizzello L, Ingrosso C, Rinaldi R, (2021). Purification of olive mill wastewater through noble metal nanoparticle synthesis: waste safe disposal and nanomaterial impact on healthy hepatic cell mitochondria Environmental Science And Pollution Research 28, 26154-26171

The exponential increase of waste derived from different human activities points out the importance of their reuse in order to create materials with specific properties that can be used for different applications. In this work, it was showed how the typical Mediterranean organic liquid waste, namely olive mill wastewater (OMWW), obtained during olive oil production, can be turned into an efficient reactive agent for the production of noble metals gold (Au) and silver nanoparticles (Ag NPs) with very well-defined physico-chemical properties. More than that, it was demonstrated that this synthetic procedure also leads to a drastic decrease of the organic pollution load of the OMWW, making it safer for environmental disposal and plants irrigation. Then, using healthy hepatic cell line mitochondria, the biological effects induced by these green metal NPs surrounded by a polyphenols shell, with the same NPs synthetized through a standard chemical colloidal reduction process, were compared, finding out that the green NPs are much safer.

JTD Keywords: antioxidants perturbation, green synthesis, gtpase dynamin-related protein 1 expression, mitochondria assessment, physico-chemical properties, Antioxidants perturbation, Green synthesis, Gtpase dynamin-related protein 1 expression, Mitochondria assessment, Physico-chemical properties, Reusability of waste