by Keyword: Cell responses
Najibi, Alexander J, Lane, Ryan S, Sobral, Miguel C, Bovone, Giovanni, Kang, Shawn, Freedman, Benjamin R, Estupinan, Joel Gutierrez, Elosegui-Artola, Alberto, Tringides, Christina M, Dellacherie, Maxence O, Williams, Katherine, Ijaz, Hamza, Muller, Soren, Turley, Shannon J, Mooney, David J, (2024). Durable lymph-node expansion is associated with the efficacy of therapeutic vaccination Nature Biomedical Engineering , 
Following immunization, lymph nodes dynamically expand and contract. The mechanical and cellular changes enabling the early-stage expansion of lymph nodes have been characterized, yet the durability of such responses and their implications for adaptive immunity and vaccine efficacy are unknown. Here, by leveraging high-frequency ultrasound imaging of the lymph nodes of mice, we report more potent and persistent lymph-node expansion for animals immunized with a mesoporous silica vaccine incorporating a model antigen than for animals given bolus immunization or standard vaccine formulations such as alum, and that durable and robust lymph-node expansion was associated with vaccine efficacy and adaptive immunity for 100 days post-vaccination in a mouse model of melanoma. Immunization altered the mechanical and extracellular-matrix properties of the lymph nodes, drove antigen-dependent proliferation of immune and stromal cells, and altered the transcriptional features of dendritic cells and inflammatory monocytes. Strategies that robustly maintain lymph-node expansion may result in enhanced vaccination outcomes. Durable and robust lymph-node expansion is associated with the efficacy of therapeutic vaccination, as shown in mice immunized via a biomaterial-based vaccine.
JTD Keywords: Antigen, Ccr2, Cell responses, Initiation, Vivo
