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Publications

by Keyword: Cell responses

Najibi, AJ, Lane, RS, Sobral, MC, Bovone, G, Kang, SW, Freedman, BR, Estupinan, JG, Elosegui-Artola, A, Tringides, CM, Dellacherie, MO, Williams, K, Ijaz, H, Müller, S, Turley, SJ, Mooney, DJ, (2024). Durable lymph-node expansion is associated with the efficacy of therapeutic vaccination Nature Biomedical Engineering 8,

Following immunization, lymph nodes dynamically expand and contract. The mechanical and cellular changes enabling the early-stage expansion of lymph nodes have been characterized, yet the durability of such responses and their implications for adaptive immunity and vaccine efficacy are unknown. Here, by leveraging high-frequency ultrasound imaging of the lymph nodes of mice, we report more potent and persistent lymph-node expansion for animals immunized with a mesoporous silica vaccine incorporating a model antigen than for animals given bolus immunization or standard vaccine formulations such as alum, and that durable and robust lymph-node expansion was associated with vaccine efficacy and adaptive immunity for 100 days post-vaccination in a mouse model of melanoma. Immunization altered the mechanical and extracellular-matrix properties of the lymph nodes, drove antigen-dependent proliferation of immune and stromal cells, and altered the transcriptional features of dendritic cells and inflammatory monocytes. Strategies that robustly maintain lymph-node expansion may result in enhanced vaccination outcomes. Durable and robust lymph-node expansion is associated with the efficacy of therapeutic vaccination, as shown in mice immunized via a biomaterial-based vaccine.

JTD Keywords: Antigen, Ccr2, Cell responses, Initiation, Vivo