by Keyword: Hepatocyte
Herrero-Gomez, A, Azagra, M, Marco-Rius, I, (2022). A cryopreservation method for bioengineered 3D cell culture models Biomedical Materials 17, 045023 
Technologies to cryogenically preserve (a.k.a. cryopreserve) living tissue, cell lines and primary cells have matured greatly for both clinicians and researchers since their first demonstration in the 1950s and are widely used in storage and transport applications. Currently, however, there remains an absence of viable cryopreservation and thawing methods for bioengineered, three-dimensional (3D) cell models, including patients' samples. As a first step towards addressing this gap, we demonstrate a viable protocol for spheroid cryopreservation and survival based on a 3D carboxymethyl cellulose scaffold and precise conditions for freezing and thawing. The protocol is tested using hepatocytes, for which the scaffold provides both the 3D structure for cells to self-arrange into spheroids and to support cells during freezing for optimal post-thaw viability. Cell viability after thawing is improved compared to conventional pellet models where cells settle under gravity to form a pseudo-tissue before freezing. The technique may advance cryobiology and other applications that demand high-integrity transport of pre-assembled 3D models (from cell lines and in future cells from patients) between facilities, for example between medical practice, research and testing facilities.
JTD Keywords: 3d cell culture, biofabrication, biomaterials, carboxymethyl cellulose, cryopreservation, hepatocytes, 3d cell culture, Biofabrication, Biomaterials, Carboxymethyl cellulose, Cell culture techniques, three dimensional, Cell survival, Cryopreservation, Freezing, Hepatocytes, Humans, Prevention, Scaffolds, Spheroids
De Matteis, V, Rizzello, L, Ingrosso, C, Rinaldi, R, (2021). Purification of olive mill wastewater through noble metal nanoparticle synthesis: waste safe disposal and nanomaterial impact on healthy hepatic cell mitochondria Environmental Science And Pollution Research 28, 26154-26171
The exponential increase of waste derived from different human activities points out the importance of their reuse in order to create materials with specific properties that can be used for different applications. In this work, it was showed how the typical Mediterranean organic liquid waste, namely olive mill wastewater (OMWW), obtained during olive oil production, can be turned into an efficient reactive agent for the production of noble metals gold (Au) and silver nanoparticles (Ag NPs) with very well-defined physico-chemical properties. More than that, it was demonstrated that this synthetic procedure also leads to a drastic decrease of the organic pollution load of the OMWW, making it safer for environmental disposal and plants irrigation. Then, using healthy hepatic cell line mitochondria, the biological effects induced by these green metal NPs surrounded by a polyphenols shell, with the same NPs synthetized through a standard chemical colloidal reduction process, were compared, finding out that the green NPs are much safer.
JTD Keywords: antioxidants perturbation, green synthesis, gtpase dynamin-related protein 1 expression, mitochondria assessment, physico-chemical properties, Antioxidants perturbation, Green synthesis, Gtpase dynamin-related protein 1 expression, Hepatocytes, Humans, Industrial waste, Metal nanoparticles, Mitochondria, Mitochondria assessment, Olea, Olive oil, Physico-chemical properties, Reusability of waste, Silver, Waste disposal, fluid, Waste water, Wastewater
Guixé-Muntet, Sergi, Ortega-Ribera, Martí, Wang, Cong, Selicean, Sonia, Andreu, Ion, Kechagia, Jenny Z., Fondevila, Constantino, Roca-Cusachs, Pere, Dufour, Jean-François, Bosch, Jaime, Berzigotti, Annalisa, Gracia-Sancho, Jordi, (2020). Nuclear deformation mediates liver cell mechanosensing in cirrhosis JHEP Reports 2, (5), 100145
Background & AimsLiver stiffness is increased in advanced chronic liver disease (ACLD) and accurately predicts prognosis in this population. Recent data suggest that extracellular matrix stiffness per se may modulate the phenotype of liver cells. We aimed at investigating the effect of matrix stiffness on the phenotype of liver cells of rats with cirrhosis, assessing its influence on their response to antifibrotic strategies and evaluating associated molecular mechanisms.
JTD Keywords: Chronic liver disease, Hepatocyte, HSC, LSEC, Stiffness
