by Keyword: cachexia
García-Lizarribar, A, Villasante, A, Lopez-Martin, JA, Flandez, M, Soler-Vázquez, MC, Serra, D, Herrero, L, Sagrera, A, Efeyan, A, Samitier, J, (2023). 3D bioprinted functional skeletal muscle models have potential applications for studies of muscle wasting in cancer cachexia Biomaterials Advances 150, 213426 
Acquired muscle diseases such as cancer cachexia are responsible for the poor prognosis of many patients suffering from cancer. In vitro models are needed to study the underlying mechanisms of those pathologies. Extrusion bioprinting is an emerging tool to emulate the aligned architecture of fibers while implementing additive manufacturing techniques in tissue engineering. However, designing bioinks that reconcile the rheological needs of bioprinting and the biological requirements of muscle tissue is a challenging matter. Here we formulate a biomaterial with dual crosslinking to modulate the physical properties of bioprinted models. We design 3D bioprinted muscle models that resemble the mechanical properties of native tissue and show improved proliferation and high maturation of differentiated myotubes suggesting that the GelMA-AlgMA-Fibrin biomaterial possesses myogenic properties. The electrical stimulation of the 3D model confirmed the contractile capability of the tissue and enhanced the formation of sarcomeres. Regarding the functionality of the models, they served as platforms to recapitulate skeletal muscle diseases such as muscle wasting produced by cancer cachexia. The genetic expression of 3D models demonstrated a better resemblance to the muscular biopsies of cachectic mouse models. Altogether, this biomaterial is aimed to fabricate manipulable skeletal muscle in vitro models in a non-costly, fast and feasible manner.Copyright © 2023 The Authors. Published by Elsevier B.V. All rights reserved.
JTD Keywords: cachexia, constructs, skeletal muscle, tissue-engineering, Bioprinting, Cachexia, Hydrogels, Skeletal muscle, Tissue-engineering
