by Keyword: Biased reptation

Castillo-Fernandez, Oscar, Salieb-Beugelaar, Georgette B., van Nieuwkasteele, Jan W., Bomer, Johan G., Arundell, Martin, Samitier, Josep, van den Berg, Albert, Eijkel, Jan C. T., (2011). Electrokinetic DNA transport in 20 nm-high nanoslits: Evidence for movement through a wall-adsorbed polymer nanogel Electrophoresis , 32, (18), 2402-2409

The electrokinetic transport behavior of lambda-DNA (48 kbp) in 20 nm-high fused-silica nanoslits in the presence of short-chain PVP is investigated. Mobility and video data show a number of phenomena that are typical of DNA transport through gels or polymer solutions, thus indicative of rigid migration obstacles in the DNA pathway. Calculations show that a several nanometer thin layer of wall-adsorbed PVP ('nano-gel') can provide such a rigid obstacle matrix to the DNA. Such ultrathin wall-adsorbed polymer layers represent a new type of matrix for electrokinetic DNA separation.

JTD Keywords: λ-DNA, Biased reptation, Caterpillar movement, Nanoslits, Pathways, PVP