by Keyword: High-throughput screening
Drainas AP, McIlwain DR, Dallas A, Chu T, Delgado-González A, Baron M, Angulo-Ibáñez M, Trejo A, Bai Y, Hickey JW, Lu G, Lu S, Pineda-Ramirez J, Anglin K, Richardson ET, Prostko JC, Frias E, Servellita V, Brazer N, Chiu CY, Peluso MJ, Martin JN, Wirz OF, Pham TD, Boyd SD, Kelly JD, Sage J, Nolan GP, Rovira-Clavé X, (2025). High-throughput multiplexed serology via the mass-spectrometric analysis of isotopically barcoded beads Nature Biomedical Engineering 9, 1117-1128 
In serology, each sample is typically tested individually, one antigen at a time. This is costly and time consuming. Serology techniques should ideally allow recurrent measurements in parallel in small sample volumes and be inexpensive and fast. Here we show that mass cytometry can be used to scale up multiplexed serology testing by leveraging polystyrene beads uniformly loaded with combinations of stable isotopes. We generated 18,480 unique isotopically barcoded beads to simultaneously detect, in a single tube with 924 serum samples, the levels of immunoglobulins G and M against 19 proteins from SARS-CoV-2 (a total of 36,960 tests in 400 nl of sample volume and 30 mu l of reaction volume). As a rapid, high-throughput and cost-effective technique, serology by mass cytometry may contribute to the effective management of public health emergencies originating from infectious diseases.
JTD Keywords: Antibodies, viral, Biolog, Covid-19, Covid-19 serological testing, Cytometer, High-throughput screening assays, Humans, Immunoglobulin g, Immunoglobulin m, Mass spectrometry, Microspheres, Polystyrenes, Sars-cov-2, Transmission
Pla-Roca, M., Altay, G., Giralt, X., Casals, A., Samitier, J., (2016). Design and development of a microarray processing station (MPS) for automated miniaturized immunoassays 
Biomedical Microdevices , 18, (4)
Here we describe the design and evaluation of a fluidic device for the automatic processing of microarrays, called microarray processing station or MPS. The microarray processing station once installed on a commercial microarrayer allows automating the washing, and drying steps, which are often performed manually. The substrate where the assay occurs remains on place during the microarray printing, incubation and processing steps, therefore the addressing of nL volumes of the distinct immunoassay reagents such as capture and detection antibodies and samples can be performed on the same coordinate of the substrate with a perfect alignment without requiring any additional mechanical or optical re-alignment methods. This allows the performance of independent immunoassays in a single microarray spot.
JTD Keywords: Automation, Customization, High-throughput screening, Immunoassays, Microarrays
