by Keyword: Neurociencia

Karkali, K., Jorba, I., Navajas, D., Martin-Blanco, E., (2022). Measuring ventral nerve cord stiffness in live flat-dissected Drosophila embryos by atomic force microscopy Star Protocols 3, 101901

Drosophila is an amenable system for addressing the mechanics of morphogenesis. We describe a workflow for characterizing the mechanical properties of its ventral nerve cord (VNC), at different developmental stages, in live, flat-dissected embryos employing atomic force microscopy (AFM). AFM is performed with spherical probes, and stiffness (Young’s modulus) is calculated by fitting force curves with Hertz’s contact model.

JTD Keywords: atomic force microscopy (afm), developmental biology, model organisms, Atomic force microscopy, Biology, Neurociencia, Neuroscience