Nanoscopy for nanomedicine


Lorenzo Albertazzi | Junior Group Leader
Pietro Delcanale | Postdoctoral Researcher
Silvia Pujals Riatós | Postdoctoral Researcher
Maria Arista Romero | PhD Student
Natàlia Feiner Gracia | PhD Student
Lisanne Van Oppen | PhD Student
Edgar Fuentes Fuentes | Masters Student
Bernat Miret Ontiveros | Masters Student
Roger Riera Brillas | Masters Student
Alis Olea Rodica | Masters Student
Christian Vila Parrondo | Masters Student
Rachel Ruth Aizen | Visiting Researcher

About

The main goal of our group is to use Super Resolution Microscopy (nanoscopy) to visualize and track in living cells and tissues self-assembled nanomaterials with therapeutic potential (nanomedicine).

TEM image of novel self-assembled nanofibers synthesized in the group.

The understanding of materials-cell interactions is the key towards the development of novel nanotechnology-based therapies for treatment of cancer and infectious diseases.
Our group aims to use a multidisciplinary approach, at the interface of chemistry, physics and biology, to develop novel nanomaterials for the treatment of cancer and infectious diseases.

We aim at the development of novel nanocarriers for drug delivery based on self-assembly, i.e. able to build themselves. Molecular self-organization is ubiquitous in the biological world and represents for us a source of inspiration for the design of nanostructures with biomedical potential. In particular we focus on the development of self-assembled nanoparticles and nanofibers able to selectively target diseased cells and deliver locally therapeutic moieties such as drugs and genetic material (e.g. DNA, siRNA, mRNA).

Right: Nanoparticles interactions with blood components imaged with conventional optical microscopy (left) and super resolution STORM microscopy (right).

A key point towards the development of novel nanotechnology-based therapies is the understanding of the behavior of nanomaterials in the complex biological environment. Here we use super resolution microscopy to track nanomaterials during their voyage in the biological environment and to visualize the interactions with blood components, immune system and target cells. We make use of a variety of super resolution techniques based on single molecule detection such a stochastic optical reconstruction microscopy (STORM), photoactivated localization microscopy (PALM), point accumulation for imaging in nanoscale topography (PAINT), and single particle tracking (SPT). These methods allow to achieve a resolution down to few nanometers and are therefore ideal to visualize nanosized synthetic objects in the biological environment. Super resolution microscopy provides a molecular picture of structure-activity relations and represent a guide towards the design of innovative materials for nanomedicine.

News/Jobs

IBEC project among 44 funded by AXA
20/04/2016

The AXA Research Fund, the international scientific philanthropy initiative of global insurer AXA, officially announced last week that it will devote €15.6m in 2016 to 44 new research projects with leading academic institutions in 16 countries.


Playing with molecular Lego to build the next generation of drug delivery vectors
11/02/2016

New IBEC junior group leader Lorenzo Albertazzi and his former colleagues at the Eindhoven University of Technology, working together with industry partner Novartis, have made a leap in drug delivery vectors by developing a new type of carrier with some groundbreaking improvements.


“Wrapping Up Pandemics in New Nanomaterials”
01/02/2016

Lorenzo Albertazzi’s research project funded by AXA, Novel approaches for Pandemic Virus Targeting Using Adaptive Polymers, is featured on the Granted Projects section of their website.


‘Submarinos’ en la sangre para llevar el medicamento a las células dañadas
06/10/2015

New IBEC junior group leader Lorenzo Albertazzi is profiled in El Mundo’s “Personajes Únicos” section this week.


Expert in novel drug delivery systems joins IBEC
08/09/2015

Dr Lorenzo Albertazzi, a nanoscientist whose research focuses on creating smart self-assembling materials for therapeutic applications, is joining IBEC this September.


Projects

International grants
Novel approaches for Pandemic Virus Targeting Using Adaptive Polymers AXA Research Fund Lorenzo Albertazzi
National grants
TARGETSTORM Nanomateriales para terapias dirigidas contra el cáncer visualizados con microscopia de súper resolución STORM (2016-2019) MINECO Retos investigación: Proyectos I+D Lorenzo Albertazzi

Publications

(See full publication list in ORCID)

Labernadie, A., Kato, T., Brugués, A., Serra-Picamal, X., Derzsi, S., Arwert, E., Weston, A., González-Tarragó, V., Elosegui-Artola, A., Albertazzi, L., Alcaraz, J., Roca-Cusachs, P., Sahai, E., Trepat, X., (2017). A mechanically active heterotypic E-cadherin/N-cadherin adhesion enables fibroblasts to drive cancer cell invasion Nature Cell Biology 19, 3, 224-237

Van Onzen, A. H. A. M., Albertazzi, L., Schenning, A. P. H. J., Milroy, L. G., Brunsveld, L., (2017). Hydrophobicity determines the fate of self-assembled fluorescent nanoparticles in cells Chemical Communications 53, 10, 1626-1629

Bakker, Maarten H., Lee, Cameron C., Meijer, E. W., Dankers, Patricia Y. W., Albertazzi, Lorenzo, (2016). Multicomponent supramolecular polymers as a modular platform for intracellular delivery ACS Nano 10, 2, 1845-1852

Beun, L. H., Albertazzi, L., Van Der Zwaag, D., De Vries, R., Cohen Stuart, M. A., (2016). Unidirectional living growth of self-assembled protein nanofibrils revealed by super-resolution microscopy ACS Nano 10, 5, 4973-4980

Garzoni, M., Baker, M. B., Leenders, C. M. A., Voets, I. K., Albertazzi, L., Palmans, A. R. A., Meijer, E. W., Pavan, G. M., (2016). Effect of H-bonding on order amplification in the growth of a supramolecular polymer in water Journal of the American Chemical Society 138, 42, 13985-13995

Aloi, Antonio, Vargas Jentzsch, Andreas, Vilanova, Neus, Albertazzi, Lorenzo, Meijer, E. W., Voets, Ilja K., (2016). Imaging nanostructures by single-molecule localization microscopy in organic solvents Journal of the American Chemical Society 138, 9, 2953-2956

da Silva, Ricardo M. P., van der Zwaag, Daan, Albertazzi, Lorenzo, Lee, Sungsoo S., Meijer, E. W., Stupp, Samuel I., (2016). Super-resolution microscopy reveals structural diversity in molecular exchange among peptide amphiphile nanofibres Nature Communications 7, 11561

De Koker, Stefaan, Cui, Jiwei, Vanparijs, Nane, Albertazzi, Lorenzo, Grooten, Johan, Caruso, Frank, De Geest, Bruno G., (2016). Engineering polymer hydrogel nanoparticles for lymph node-targeted delivery Angewandte Chemie - International Edition 55, 4, 1334-1339

Li, Hui, Fierens, Kaat, Zhang, Zhiyue, Vanparijs, Nane, Schuijs, Martijn J., Van Steendam, Katleen, Feiner Gracia, Natàlia, De Rycke, Riet, De Beer, Thomas, De Beuckelaer, Ans, De Koker, Stefaan, Deforce, Dieter, Albertazzi, Lorenzo, Grooten, Johan, Lambrecht, Bart N., De Geest, Bruno G., (2016). Spontaneous protein adsorption on graphene oxide nanosheets allowing efficient intracellular vaccine protein delivery ACS Applied Materials & Interfaces 8, 2, 1147-1155

van der Zwaag, Daan, Vanparijs, Nane, Wijnands, Sjors, De Rycke, Riet, De Geest, Bruno G., Albertazzi, Lorenzo, (2016). Super resolution imaging of nanoparticles cellular uptake and trafficking ACS Applied Materials & Interfaces 8, 10, 6391-6399

Beuwer, Michael A., Knopper, M. F., Albertazzi, Lorenzo, van der Zwaag, Daan, Ellenbroek, Wouter G., Meijer, E. W., Prins, Menno W. J., Zijlstra, Peter, (2016). Mechanical properties of single supramolecular polymers from correlative AFM and fluorescence microscopy Polymer Chemistry 7, 47, 7260-7268

Equipment

  • Nikon NSTORM Super Resolution Microscope
  • Super Resolution microscopy
  • Single particles tracking
  • TIRF fluorescence imaging

Collaborations

  • Roey Amir
    Tel Aviv University, Israel
  • Mika Linden
    Ulm University, Germany
  • Ilja Voets
    Eindhoven University of Technology, The Netherlands
  • Giovanni Pavan
    SUPSI, Switzerland
  • Bruno De Geest
    University of Ghent, Belgium
  • Salvador Borros
    IQS, Barcelona

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