Publications

Silica-based nanoparticles have been extensively investigated as advanced bioimaging probes and smart nanocarriers for the development of nanomedicine because of their unique properties. The deep understanding of nanoparticle-biological system (nanobiology) interactions is an important determinant for the success of nanomedicine. In this Review, we focus on the assessment of significant nanobio interactions along with the intracellular fates of silica-based nanoparticles by advanced microscopy and link this information to their recent biomedical applications. Initially, the fundamental concepts of the dynamic interactions between nanoparticles and proteins followed by cells and key factors that influence these nanobio interactions are briefly introduced. Furthermore, the basic principles of advanced imaging modalities for the analysis of nanobio interactions in this study are described. Next, the utilization of advanced imaging for the characterization of protein coronas and monitoring the cellular internalization and intracellular trafficking/fate of individual nanoparticles are comprehensively summarized. Finally, recent biomedical applications of nanoparticles in bioimaging and drug delivery are discussed.

JTD Keywords: Adsorption, Cellular uptake, Desig, Drug-delivery, Mesoporous organosilica nanoparticles, Nanocapsules, Protein corona, Size, Superresolution microscopy, Surface-properties

The use of nanocarriers in medicine, so-called nanomedicine, is one of the most innovative strategies for targeting drugs at the action site and increasing their activity index and effectiveness. Phytomedicine is the oldest traditional method used to treat human diseases and solve health problems. The recent literature on the treatment of malaria infections using nanodelivery systems and phytodrugs or supplements has been analyzed. For the first time, in the present review, a careful look at the considerable potential of nanomedicine in promoting phytotherapeutic efficacy was done, and its key role in addressing a translation through a significant reduction of the current burden of malaria in many parts of the world has been underlined.

JTD Keywords: antiplasmodial activity, bioavailability, chloroquine, combination therapy, discovery, drug-delivery, drug-delivery systems, nanocapsules, nanomedicine, natural molecules, pharmacokinetics, phytomedicine, plasmodium-falciparum, Artemisinin-based combination therapy, Drug-delivery systems, Nanomedicine, Natural molecules, Phytomedicine, Solid lipid nanoparticles

Biodegradable periodic mesoporous organosilica nanoparticles (nanoPMOs) are widely used as responsive drug delivery platforms for targeted chemotherapy of cancer. However, the evaluation of their properties such as surface functionality and biodegradability is still challenging, which has a significant impact on the efficiency of chemotherapy. In this study, we have applied direct stochastic optical reconstruction microscopy (dSTORM), a single-molecule super-resolution microscopy technique, to quantify the degradation of nanoPMOs triggered by glutathione and the multivalency of antibody-conjugated nanoPMOs. Subsequently, the effect of these properties on cancer cell targeting, drug loading and release capability, and anticancer activity is also studied. Due to the higher spatial resolution at the nanoscale, dSTORM imaging is able to reveal the structural properties (i.e., size and shape) of fluorescent and biodegradable nanoPMOs. The quantification of nanoPMOs' biodegradation using dSTORM imaging demonstrates their excellent structure-dependent degradation behavior at a higher glutathione concentration. The surface functionality of anti-M6PR antibody-conjugated nanoPMOs as quantified by dSTORM imaging plays a key role in prostate cancer cell labeling: oriented antibody is more effective than random ones, while high multivalency is also effective. The higher biodegradability and cancer cell-targeting properties of nanorods conjugated with oriented antibody (EAB4H) effectively deliver the anticancer drug doxorubicin to cancer cells, exhibiting potent anticancer effects.

JTD Keywords: drug-delivery, nanocapsules, nanomaterials, nanomedicine, release, Silica nanoparticles