Cellular and respiratory biomechanics


Daniel Navajas Navarro | Group Leader
Jordi Alcaraz Casademunt | Postdoctoral Researcher
Jordi Otero Díaz | Postdoctoral Researcher
Noelia Campillo Agulló | PhD Student
Ignasi Jorba Masdéu | PhD Student
Maeba Polo Tortola | Laboratory Technician

About

The goal of our research is to gain a deeper understanding of cellular and respiratory biomechanics to improve the diagnosis and treatment of respiratory diseases.

The work is organized in two interrelated areas, focused on respiratory mechanics at both the systemic and the cellular level. We use basic and translational approaches in a multidisciplinary framework involving close cooperation with clinical groups.

Mechanical mapping and imaging of the extracellular matrix of a slice of decellularized mouse lung obtained by the combination of bright field (A), immunofluorescence microscopy (B), and atomic force microscopy (C – F).

Our current research interest is focused on the study of cell-matrix mechanical cross-talk for tissue engineering and regenerative medicine. Cells sense and actively respond to the biophysical features of their microenvironment. Mechanical properties of the extracellular matrix regulate critical cell processes such as contraction, migration, proliferation, gene expression and differentiation. We use atomic force microscopy and other cutting-edge biophysical techniques to study the mechanical properties of the extracellular matrix and their impact in cell behavior. We have implemented protocols to decellularize different soft tissues. This innovative approach allowed us to reveal the local mechanical properties of the lung and heart extracellular matrix. By seeding cells in these scaffolds we study the impact of the mechanical features of the microenvironment on stem cell engraftment and differentiation onto lung and heart phenotypes. We produce lab-on-chip devices mimicking the native cell microenvironment to investigate mechanical signaling driving stem cell differentiation under precisely controlled conditions. Using 3D bioprinters we integrate stem cells into synthetic and extracellular matrix hydrogels to fabricate tissue patches as an innovative approach to regenerate ventricular scars resulting from heart infarct. Organ biofabrication reengineered from decellularized tissue scaffolds offers a promising alternative for transplantation. We develop improved bioreactors mimicking breathing and blood perfusion to biofabricate lungs by seeding stem cells into acellular lung scaffolds.

News/Jobs

Researchers reveal a potential cancer repressor function in a key protein
01/12/15

In a paper published in Nature Cell Biology, IBEC junior group leader Pere Roca Cusachs and his collaborators at Columbia University and Singapore’s Mechanobiology Institute reveal the potential of a protein found in cell cytoskeletons as a repressor of cancer.


How cells cope with stress and strain
15/06/15

A study by the Institute for Bioengineering of Catalonia (IBEC) reveals how cells withstand breakage during the constant changes in shape and volume experienced in most biological processes.


IBEC hosts CellMech2015 this week
13/05/15

The 6th European CellMech Meeting, which is being hosted by IBEC, started this morning at the University of Barcelona’s Faculty of Medicine.


A new mechanism in cell communication that promotes cancer metastasis
01/04/15

At a press conference at the Obra Social “la Caixa”’s Palau Macaya earlier today, Xavier Trepat, group leader at the Institute for Bioengineering of Catalonia (IBEC), Enric Banda, director of the department of Science and Environment of the Obra Social ”la Caixa”, and Josep Samitier, director of IBEC, described a study published in Nature Cell Biology which sheds new light on how to control metastasis.


IBEC research on Nature Materials cover
22/05/14

The cover of Nature Materials featuring the Cellular and Respiratory Biomechanics group’s recent paper on how mammary cells detect tissue stiffening is out now.


“Descubren un mecanismo celular clave en el desarrollo del cáncer de mama”
05/05/14

Alberto Elosegui and Pere Roca-Cusachs’ Nature Materials paper gets lots of press coverage in newspapers, TV and radio today. In the study, they describe for the first time how mammary cells detect tissue stiffening, which is key to the development of breast cancer.


Researchers uncover a fundamental mechanism in breast cancer
05/05/14

A study by IBEC researchers reveals in Nature Materials how mammary cells detect tissue stiffening, which is key to the development of breast cancer.


Marató money for IBEC project
12/11/2013

Junior group leader Pere Roca-Cusachs has received funding from 2012’s La Marató de TV3 for his project “Stromal stiffness in Tumor Progression”.


IBEC research on a journal cover again
27/09/2013

The October cover of Current Opinion in Cell Biology (the reference opinion journal in cell biology, with impact factor 12) features research by IBEC’s Pere Roca and Xavier Trepat, who together with Raimon Sunyer from the UB have published a review paper in the issue, “Mechanical guidance of cell migration: lessons from chemotaxis”.


Stepping our understanding of cellular function up a gear
21/03/2013

An IBEC researcher and his collaborators uncover the crucial role of two molecules in enabling cells to communicate with their environment.


The mattress test: cells do it too
22/03/2012

Just as people are picky about the type of mattress they want to sleep on – not too hard, but not so soft either – so are cells. In fact, the rigidity of the cellular environment is so important that it can be the determining factor of whether a stem cell will differentiate into bone or fat, for example – or whether a cell behaves normally or turns cancerous.


Discovery of the Functional Differences Between 2 Integrins in the Adhesion of the Cell
22/09/2009

The adhesion of cells to the various components of their environment is mediated mainly by molecules known as integrins. Mediate adhesion with fibronectin, two integrins, α5β1 and αvβ3, which is present in the extracellular matrix. But why are 2 molecules involved in the adhesion to a single extracellular component? An article published on-line in Proceedings of the National Academy of Sciences (PNAS), whose main author is Pere Roca-Cusachs, an IBEC researcher, offers the first experimental answer to this question.


Feeling and pressing the cytoplasm
10/07/2009

IBEC researcher in collaboration with a Harvard-led team in PNAS


Researcher at IBEC publishes in Nature Physics
06/05/2009

An article by Dr. Xavier Trepat, senior researcher of IBEC´s Cellular and respiratory biomechanics group and the Department of Physiology Sciences of the University of Barcelona, contributes for the first time an experimental answer to the question of how cells move during biological processes as diverse as the development, metastasis, or regeneration of tissues.

Projects

National projects
Señalización mécanica en la diferenciación de células madre en el pulmón. Modelo pulmón-en-un-chip FIS-Proyectos de investigación en salud Daniel Navajas
Precondicionamento biofísico de células madre mesenquimales para el tratamiento de la lesión pulmonar aguda provocada por sobreventilación en modelo animal (2015-2017) FIS-MINECO (PI14/00280) Daniel Navajas

Publications

Hernández-Vega, Amayra, Marsal, María, Pouille, Philippe-Alexandre, Tosi, Sébastien, Colombelli, Julien, Luque, Tomás, Navajas, Daniel, Pagonabarraga, Ignacio, Martín-Blanco, Enrique, (2017). Polarized cortical tension drives zebrafish epiboly movements EMBO Journal 36, 1, 25-41

Campillo, N., Torres, M., Vilaseca, A., Nonaka, P. N., Gozal, D., Roca-Ferrer, J., Picado, C., Montserrat, J. M., Farré, R., Navajas, D., Almendros, I., (2017). Role of cyclooxygenase-2 on intermittent hypoxia-induced lung tumor malignancy in a mouse model of sleep apnea Scientific Reports 7, 44693 [Open Access]

Jorba, I., Uriarte, J. J., Campillo, N., Farré, R., Navajas, D., (2017). Probing micromechanical properties of the extracellular matrix of soft tissues by atomic force microscopy Journal of Cellular Physiology 232, 1, 19-26

Jorba, I., Menal, M. J., Torres, M., Gozal, D., Piñol-Ripoll, G., Colell, A., Montserrat, J. M., Navajas, D., Farré, R., Almendros, I., (2017). Ageing and chronic intermittent hypoxia mimicking sleep apnea do not modify local brain tissue stiffness in healthy mice Journal of the Mechanical Behavior of Biomedical Materials 71, 106-113

Isetta, V., Torres, M., González, K., Ruiz, C., Dalmases, M., Embid, C., Navajas, D., Farré, R., Montserrat, J. M., (2017). A New mHealth application to support treatment of sleep apnoea patients Journal of Telemedicine and Telecare 23, 1, 14-18

Giménez, A., Uriarte, J. J., Vieyra, J., Navajas, D., Alcaraz, J., (2017). Elastic properties of hydrogels and decellularized tissue sections used in mechanobiology studies probed by atomic force microscopy Microscopy Research and Technique 80, 1, 85-96

Farré, R., Navajas, D., Montserrat, J. M., (2016). Technology for noninvasive mechanical ventilation: Looking into the black box ERS Monograph 2, 1, 00004

Wolfenson, Haguy, Meacci, Giovanni, Liu, Shuaimin, Stachowiak, Matthew R., Iskratsch, Thomas, Ghassemi, Saba, Roca-Cusachs, Pere, Oshaughnessy, Ben, Hone, James, Sheetz, Michael P., (2016). Tropomyosin controls sarcomere-like contractions for rigidity sensing and suppressing growth on soft matrices Nature Cell Biology 18, 33-42

Campillo, N., Jorba, I., Schaedel, L., Casals, B., Gozal, D., Farré, R., Almendros, I., Navajas, D., (2016). A novel chip for cyclic stretch and intermittent hypoxia cell exposures mimicking obstructive sleep apnea Frontiers in Physiology 7, Article 319

Nonaka, Paula N., Uriarte, Juan J., Campillo, Noelia, Oliveira, Vinicius R., Navajas, Daniel, Farré, Ramon, (2016). Lung bioengineering: physical stimuli and stem/progenitor cell biology interplay towards biofabricating a functional organ Respiratory Research 17, 1, 161 [Open Access]

Farré, R., Navajas, D., (2016). Forced oscillation: A poorly exploited tool for simply assessing respiratory function in children Respirology 21, 6, 982-983

Uriarte, J. J., Meirelles, T., Del Blanco, D. G., Nonaka, P. N., Campillo, N., Sarri, E., Navajas, D., Egea, G., Farré, R., (2016). Early impairment of lung mechanics in a murine model of marfan syndrome PLoS ONE 11, 3, e0152124 [Open Access]

Isetta, V., Montserrat, J. M., Santano, R., Wimms, A. J., Ramanan, D., Woehrle, H., Navajas, D., Farré, R., (2016). Novel approach to simulate sleep apnea patients for evaluating positive pressure therapy devices PLoS ONE 11, 3, e0151530 [Open Access]

Valero, C., Navarro, B., Navajas, D., García-Aznar, J. M., (2016). Finite element simulation for the mechanical characterization of soft biological materials by atomic force microscopy Journal of the Mechanical Behavior of Biomedical Materials 62, 222-235

da Palma, R. K., Nonaka, P. N., Campillo, N., Uriarte, J. J., Urbano, J. J., Navajas, D., Farré, R., Oliveira, L. V. F., (2016). Behavior of vascular resistance undergoing various pressure insufflation and perfusion on decellularized lungs Journal of Biomechanics 49, 7, 1230-1232

Bazellières, Elsa, Conte, Vito, Elosegui, Alberto, Serra-Picamal, Xavier, Bintanel-Morcillo, María, Roca-Cusachs, Pere, Muñoz, José J., Sales-Pardo, Marta, Guimerà, Roger, Trepat, Xavier, (2015). Control of cell-cell forces and collective cell dynamics by the intercellular adhesome Nature Cell Biology 17, 4, 409-420

Kosmalska, A. J., Casares, L., Elosegui, A., Thottacherry, J. J., Moreno-Vicente, R., González-Tarragó, V., Del Pozo, M. Á, Mayor, S., Arroyo, M., Navajas, D., Trepat, X., Gauthier, N. C., Roca-Cusachs, P., (2015). Physical principles of membrane remodelling during cell mechanoadaptation Nature Communications 6, 7292

Stanisavljevic, J., Loubat-Casanovas, J., Herrera, M., Luque, T., Peña, R., Lluch, A., Albanell, J., Bonilla, F., Rovira, A., Peña, C., Navajas, D., Rojo, F., García De Herreros, A., Baulida, J., (2015). Snail1-expressing fibroblasts in the tumor microenvironment display mechanical properties that support metastasis Cancer Research 75, 2, 284-295

Crosas-Molist, E., Meirelles, T., López-Luque, J., Serra-Peinado, C., Selva, J., Caja, L., Gorbenko Del Blanco, D., Uriarte, J. J., Bertran, E., Mendizábal, Y., Hernández, V., García-Calero, C., Busnadiego, O., Condom, E., Toral, D., Castellà, M., Forteza, A., Navajas, D., Sarri, E., Rodríguez-Pascual, F., Dietz, H. C., Fabregat, I., Egea, G., (2015). Vascular smooth muscle cell phenotypic changes in patients with marfan syndrome Arteriosclerosis, Thrombosis, and Vascular Biology 35, 4, 960-972

Perea-Gil, I., Uriarte, J. J., Prat-Vidal, C., Gálvez-Montón, C., Roura, S., Llucià-Valldeperas, A., Soler-Botija, C., Farré, R., Navajas, D., Bayes-Genis, A., (2015). In vitro comparative study of two decellularization protocols in search of an optimal myocardial scaffold for recellularization American Journal of Translational Research 7, 3, 558-573

Torres, M., Rojas, M., Campillo, N., Cardenes, N., Montserrat, J. M., Navajas, D., Farré, R., (2015). Parabiotic model for differentiating local and systemic effects of continuous and intermittent hypoxia Journal of Applied Physiology 118, 1, 42-47

da Palma, R. K., Campillo, N., Uriarte, J. J., Oliveira, L. V. F., Navajas, D., Farré, R., (2015). Pressure- and flow-controlled media perfusion differently modify vascular mechanics in lung decellularization Journal of the Mechanical Behavior of Biomedical Materials 49, 69-79

da Palma, R. K., Farré, R., Montserrat, J. M., Gorbenko Del Blanco, D., Egea, G., de Oliveira, L. V. F., Navajas, D., Almendros, I., (2015). Increased upper airway collapsibility in a mouse model of Marfan syndrome Respiratory Physiology & Neurobiology 207, 58-60

Isetta, V., León, C., Torres, M., Embid, C., Roca, J., Navajas, D., Farré, R., Montserrat, J. M., (2014). Telemedicine-based approach for obstructive sleep apnea management: Building evidence Interactive Journal of Medical Research 3, 1, e6

Elosegui, A., Bazellières, E., Allen, M. D., Andreu, I., Oria, R., Sunyer, R., Gomm, J. J., Marshall, J. F., Jones, J. L., Trepat, X., Roca-Cusachs, P., (2014). Rigidity sensing and adaptation through regulation of integrin types Nature Materials 13, 6, 631-637

Andreu, I., Luque, T., Sancho, A., Pelacho, B., Iglesias-García, O., Melo, E., Farré, R., Prósper, F., Elizalde, M. R., Navajas, D., (2014). Heterogeneous micromechanical properties of the extracellular matrix in healthy and infarcted hearts Acta Biomaterialia 10, 7, 3235-3242

Dalmases, M., Torres, M., Márquez-Kisinousky, L., Almendros, I., Planas, A. M., Embid, C., Martínez-Garcia, M. A., Navajas, D., Farré, R., Montserrat, J. M., (2014). Brain tissue hypoxia and oxidative stress induced by obstructive apneas is different in young and aged rats Sleep 37, 7, 1249-1256

Melo, E., Garreta, E., Luque, T., Cortiella, J., Nichols, J., Navajas, D., Farré, R., (2014). Effects of the decellularization method on the local stiffness of acellular lungs Tissue Engineering Part C: Methods 20, 5, 412-422

Melo, E., Cárdenes, N., Garreta, E., Luque, T., Rojas, M., Navajas, D., Farré, R., (2014). Inhomogeneity of local stiffness in the extracellular matrix scaffold of fibrotic mouse lungs Journal of the Mechanical Behavior of Biomedical Materials 37, 186-195

Uriarte, J. J., Nonaka, P. N., Campillo, N., Palma, R. K., Melo, E., de Oliveira, L. V. F., Navajas, D., Farré, R., (2014). Mechanical properties of acellular mouse lungs after sterilization by gamma irradiation Journal of the Mechanical Behavior of Biomedical Materials 40, 168-177

Nonaka, P. N., Campillo, N., Uriarte, J. J., Garreta, E., Melo, E., de Oliveira, L. V. F., Navajas, D., Farré, R., (2014). Effects of freezing/thawing on the mechanical properties of decellularized lungs Journal of Biomedical Materials Research - Part A 102, 2, 413-419

Nonaka, P. N., Uriarte, J. J., Campillo, N., Melo, E., Navajas, D., Farré, R., Oliveira, L. V. F., (2014). Mechanical properties of mouse lungs along organ decellularization by sodium dodecyl sulfate Respiratory Physiology & Neurobiology 200, 1-5

Navajas, D., Dellacà , R. L., Farré, R., (2014). Forced oscillation technique Springer-Verlag Mailand New Insights from New Technologies: Second Edition, 137-148

Roca-Cusachs, P., Del Rio, A., Puklin-Faucher, E., Gauthier, N. C., Biais, N., Sheetz, M. P., (2013). Integrin-dependent force transmission to the extracellular matrix by Proceedings of the National Academy of Sciences of the United States of America 110, 15, E1361-E1370

Roca-Cusachs, P., Sunyer, R., Trepat, X., (2013). Mechanical guidance of cell migration: lessons from chemotaxis Current Opinion in Cell Biology 25, 5, 543-549

Luque, T., Melo, E., Garreta, E., Cortiella, J., Nichols, J., Farré, R., Navajas, D., (2013). Local micromechanical properties of decellularized lung scaffolds measured with atomic force microscopy Acta Biomaterialia 9, 6, 6852-6859

Farré, R., Navajas, D., Montserrat, J. M., (2013). Is there an optimal nasal pressure for treating obstructive sleep apnea-and if so, what is it? Sleep 36, 4, 463-464

Isetta, V., Lopez-Agustina, C., Lopez-Bernal, E., Amat, M., Vila, M., Valls, C., Navajas, D., Farre, R., (2013). Cost-effectiveness of a new internet-based monitoring tool for neonatal post-discharge home care Journal of Medical Internet Research 15, 2, e38

Marí-Buyé, N., Luque, T., Navajas, D., Semino, C. E., (2013). Development of a three-dimensional bone-like construct in a soft self-assembling peptide matrix Tissue Engineering Part A 19, 7-8, 870-881

Puig, F., Fuster, G., Adda, M., Blanch, L., Farre, R., Navajas, D., Artigas, A., (2013). Barrier-protective effects of activated protein C in human alveolar epithelial cells PLoS ONE 8, 2, e56965

Peñuelas, O., Melo, E., Sánchez, C., Sánchez, I., Quinn, K., Ferruelo, A., Pérez-Vizcaíno, F., Esteban, A., Navajas, D., Nin, N., Lorente, J. A., Farré, R., (2013). Antioxidant effect of human adult adipose-derived stromal stem cells in alveolar epithelial cells undergoing stretch Respiratory Physiology & Neurobiology 188, 1, 1-8

Torres, M., Montserrat, J. M., Pavía, J., Dalmases, M., Ros, D., Fernandez, Y., Barbé, F., Navajas, D., Farré, R., (2013). Chronic intermittent hypoxia preserves bone density in a mouse model of sleep apnea Respiratory Physiology & Neurobiology 189, 3, 646-648

Almendros, I., Montserrat, J. M., Torres, M., Dalmases, M., Cabañas, M. L., Campos-Rodríguez, F., Navajas, D., Farré, R., (2013). Intermittent hypoxia increases melanoma metastasis to the lung in a mouse model of sleep apnea Respiratory Physiology & Neurobiology 186, 3, 303-307

Jané, R., Lazaro, J., Ruiz, P., Gil, E., Navajas, D., Farre, R., Laguna, P., (2013). Obstructive Sleep Apnea in a rat model: Effects of anesthesia on autonomic evaluation from heart rate variability measures CinC 2013 IEEE , 1011-1014

Govoni, Leonardo, Dellaca, Raffaele L., Penuelas, Oscar, Bellani, Giacomo, Artigas, Antonio, Ferrer, Miquel, Navajas, Daniel, Pedotti, Antonio, Farre, Ramon, (2012). Actual performance of mechanical ventilators in ICU: a multicentric quality control study Medical Devices: Evidence and Research 5, 111-119

Almendros, Isaac, Carreras, Alba, Montserrat, Josep M., Gozal, David, Navajas, Daniel, Farre, Ramon, (2012). Potential role of adult stem cells in obstructive sleep apnea Frontiers in Neurology 3, 1-6

Ghassemi, S., Meacci, G., Liu, S., Gondarenko, A. A., Mathur, A., Roca-Cusachs, P., Sheetz, M. P., Hone, J., (2012). Cells test substrate rigidity by local contractions on submicrometer pillars Proceedings of the National Academy of Sciences of the United States of America 109, 14, 5328-5333

Almendros, I., Montserrat, J. M., Ramírez, J., Torres, M., Duran-Cantolla, J., Navajas, D., Farré, R., (2012). Intermittent hypoxia enhances cancer progression in a mouse model of sleep apnoea European Respiratory Journal 39, 1, 215-217

Chimenti, L., Luque, T., Bonsignore, M. R., Ramirez, J., Navajas, D., Farre, R., (2012). Pre-treatment with mesenchymal stem cells reduces ventilator-induced lung injury European Respiratory Journal 40, 4, 939-948

Roca-Cusachs, P., Iskratsch, T., Sheetz, M. P., (2012). Finding the weakest link: exploring integrin-mediated mechanical molecular pathways Journal of Cell Science 125, 13, 3025-3038

Acerbi, I., Luque, T., Giménez, A., Puig, M., Reguart, N., Farré, R., Navajas, D., Alcaraz, J., (2012). Integrin-specific mechanoresponses to compression and extension probed by cylindrical flat-ended afm tips in lung cells PLoS ONE 7, 2, e32261

Almendros, I., Montserrat, J. M., Torres, M., Bonsignore, M. R., Chimenti, L., Navajas, D., Farre, R., (2012). Obesity and intermittent hypoxia increase tumor growth in a mouse model of sleep apnea Sleep Medicine 13, 10, 1254-1260

Guamán, Ana V., Carreras, Alba, Calvo, Daniel, Agudo, Idoya, Navajas, Daniel, Pardo, Antonio, Marco, Santiago, Farré, Ramon, (2012). Rapid detection of sepsis in rats through volatile organic compounds in breath Journal of Chromatography B 881-882, 76-82

Tsapikouni, T., Garreta, E., Melo, E., Navajas, D., Farré, R., (2012). A bioreactor for subjecting cultured cells to fast-rate intermittent hypoxia Respiratory Physiology & Neurobiology 182, 1, 47-52

Waters, C.M., Roan, E., Navajas, D., (2012). Mechanobiology in lung epithelial cells: Measurements, perturbations, and responses John Wiley & Sons, Inc. 2, 1, 1-29

Gauthier, Nils C., Fardin, Marc Antoine, Roca-Cusachs, Pere, Sheetz, Michael P., (2011). Temporary increase in plasma membrane tension coordinates the activation of exocytosis and contraction during cell spreading Proceedings of the National Academy of Sciences of the United States of America 108, 35, 14467-14472

Almendros, Isaac, Farre, Ramon, Planas, Anna M., Torres, Marta, Bonsignore, Maria R., Navajas, Daniel, Montserrat, Josep M., (2011). Tissue oxygenation in brain, muscle, and fat in a rat model of sleep apnea: Differential effect of obstructive apneas and intermittent hypoxia Sleep 34, 8, 1127-1133

Almendros, I., Farré, R., Torres, M., Bonsignore, M. R., Dalmases, M., Ramírez, J., Navajas, D., Montserrat, J. M., (2011). Early and mid-term effects of obstructive apneas in myocardial injury and inflammation Sleep Medicine 12, 10, 1037-1040

Dellaca, Raffaele, Montserrat, Josep M., Govoni, Leonardo, Pedotti, Antonio, Navajas, Daniel, Farre, Ramon, (2011). Telemetric CPAP titration at home in patients with sleep apnea-hypopnea syndrome Sleep Medicine 12, 2, 153-157

Cagido, Viviane Ramos, Zin, Walter Araujo, Ramirez, Jose, Navajas, Daniel, Farre, Ramon, (2011). Alternating ventilation in a rat model of increased abdominal pressure Respiratory Physiology & Neurobiology 175, 3, 310-315

Carreras, Alba, Wang, Yang, Gozal, David, Montserrat, Josep M., Navajas, Daniel, Farre, Ramon, (2011). Non-invasive system for applying airway obstructions to model obstructive sleep apnea in mice Respiratory Physiology & Neurobiology 175, 1, 164-168

A. Mathur, P. Roca-Cusachs, O. M. Rossier, S. J. Wind, M. P. Sheetz, J. Hone, (2011). New approach for measuring protrusive forces in cells Journal of Vacuum Science & Technology B: Microelectronics and Nanometer Structures 29, 6, 06FA02

Trepat, X., Fabry, B., Fredberg, J. J., (2010). Pulling it together in three dimensions Nature Methods 7, 12, 963-965

Moore, S. W., Roca-Cusachs, P., Sheetz, M. P., (2010). Stretchy proteins on stretchy substrates: The important elements of integrin-mediated rigidity sensing Developmental Cell 19, 2, 194-206

Angelini, T. E., Hannezo, E., Trepat, X., Fredberg, J. J., Weitz, D. A., (2010). Cell migration driven by cooperative substrate deformation patterns Physical Review Letters 104, 16, 168104

Park, C. Y., Tambe, D., Alencar, A. M., Trepat, X., Zhou, E. H., Millet, E., Butler, J. P., Fredberg, J. J., (2010). Mapping the cytoskeletal prestress The American Journal of Physiology - Cell Physiology 298, 5, C1245-C1252

Iranzo, A., Isetta, V., Molinuevo, J. L., Serradell, M., Navajas, D., Farre, R., Santamaria, J., (2010). Electroencephalographic slowing heralds mild cognitive impairment in idiopathic REM sleep behavior disorder Sleep Medicine 11, 6, 534-539

Almendros, I., Montserrat, J. M., Torres, M., Gonzalez, C., Navajas, D., Farre, R., (2010). Changes in oxygen partial pressure of brain tissue in an animal model of obstructive apnea Respiratory Research 11, 3, 1-6

Carreras, A., Rojas, M., Tsapikouni, T., Montserrat, J. M., Navajas, D., Farre, R., (2010). Obstructive apneas induce early activation of mesenchymal stem cells and enhancement of endothelial wound healing Respiratory Research 11, 91, 1-7

Carreras, Alba, Almendros, Isaac, Montserrat, Josep M., Navajas, Daniel, Farre, Ramon, (2010). Mesenchymal stem cells reduce inflammation in a rat model of obstructive sleep apnea Respiratory Physiology & Neurobiology 172, 3, 210-212

Trepat, X., Wasserman, M. R., Angelini, T. E., Millet, E., Weitz, D. A., Butler, J. P., Fredberg, J. J., (2009). Physical forces during collective cell migration Nature Physics 5, 6, 426-430

Roca-Cusachs, P., Gauthier, N. C., del Rio, A., Sheetz, M. P., (2009). Clustering of alpha(5)beta(1) integrins determines adhesion strength whereas alpha(v)beta(3) and talin enable mechanotransduction Proceedings of the National Academy of Sciences of the United States of America 106, 38, 16245-16250

Zhou, E. H., Trepat, X., Park, C. Y., Lenormand, G., Oliver, M. N., Mijailovich, S. M., Hardin, C., Weitz, D. A., Butler, J. P., Fredberg, J. J., (2009). Universal behavior of the osmotically compressed cell and its analogy to the colloidal glass transition Proceedings of the National Academy of Sciences of the United States of America 106, 26, 10632-10637

Farre, R., Navajas, D., (2009). Quality control: A necessary, but sometimes overlooked, tool for improving respiratory medicine European Respiratory Journal 33, 4, 722-723

Carreras, A., Almendros, I., Acerbi, I., Montserrat, J. M., Navajas, D., Farre, R., (2009). Obstructive apneas induce early release of mesenchymal stem cells into circulating blood Sleep 32, 1, 117-119

Krishnan, R., Park, C. Y., Lin, Y. C., Mead, J., Jaspers, R. T., Trepat, X., Lenormand, G., Tambe, D., Smolensky, A. V., Knoll, A. H., Butler, J. P., Fredberg, J. J., (2009). Reinforcement versus fluidization in cytoskeletal mechanoresponsiveness PLoS ONE 4, 5, e5486

Sellares, J., Acerbi, I., Loureiro, H., Dellaca, R. L., Ferrer, M., Torres, A., Navajas, D., Farre, R., (2009). Respiratory impedance during weaning from mechanical ventilation in a mixed population of critically ill patients British Journal of Anaesthesia 103, 6, 828-832

Sunyer, R., Trepat, X., Fredberg, J. J., Farre, R., Navajas, D., (2009). The temperature dependence of cell mechanics measured by atomic force microscopy Physical Biology 6, 2, 25009

Sunyer, R., Ritort, F., Farre, R., Navajas, D., (2009). Thermal activation and ATP dependence of the cytoskeleton remodeling dynamics Physical Review E 79, 5, 51920

Puig, F., Gavara, N., Sunyer, R., Carreras, A., Farre, R., Navajas, D., (2009). Stiffening and contraction induced by dexamethasone in alveolar epithelial cells Experimental Mechanics 49, 1, 47-55

Dellaca, R. L., Gobbi, A., Govoni, L., Navajas, D., Pedotti, A., Farre, R., (2009). A novel simple Internet-based system for real time monitoring and optimizing home mechanical ventilation International Conference on Ehealth, Telemedicine, and Social Medicine: Etelemed 2009, Proceedings IEEE Compuer Soc , 209-215

Trepat, X., Farré, R., (2008). Alveolar permeability and stretch: Too far, too fast European Respiratory Journal 32, 4, 826-828

Almendros, I., Carreras, A., Ramirez, J., Montserrat, J. M., Navajas, D., Farre, R., (2008). Upper airway collapse and reopening induce inflammation in a sleep apnoea model European Respiratory Journal 32, 2, 399-404

Gavara, N., Roca-Cusachs, P., Sunyer, R., Farre, R., Navajas, D., (2008). Mapping cell-matrix stresses during stretch reveals inelastic reorganization of the cytoskeleton Biophysical Journal 95, 1, 464-471

Roca-Cusachs, P., Alcaraz, J., Sunyer, R., Samitier, J., Farre, R., Navajas, D., (2008). Micropatterning of single endothelial cell shape reveals a tight coupling between nuclear volume in G1 and proliferation Biophysical Journal 94, 12, 4984-4995

Crespo, C., Gallego, J., Cot, A., Falcón, C., Bullich, S., Pareto, D., Aguiar, P., Sempau, J., Lomeña, F., Calviño, F., Pavía, J., Ros, D., (2008). Quantification of dopaminergic neurotransmission SPECT studies with 123I-labelled radioligands. A comparison between different imaging systems and data acquisition protocols using Monte Carlo simulation European Journal of Nuclear Medicine and Molecular Imaging 35, 7, 1334-1342

Almendros, I., Acerbi, I., Vilaseca, I., Montserrat, J. M., Navajas, D., Farre, R., (2008). Continuous positive airway pressure (CPAP) induces early nasal inflammation Sleep 31, 1, 127-131

Farre, R., Montserrat, J. M., Navajas, D., (2008). Morbidity due to obstructive sleep apnea: insights from animal models Current Opinion in Pulmonary Medicine 14, 6, 530-536

Farre, R., Montserrat, J. M., Navajas, D., (2008). Assessment of upper airway mechanics during sleep Respiratory Physiology & Neurobiology 163, 1-3, 74-81

Almendros, I., Gutierrez, P. T., Closa, D., Navajas, D., Farre, R., (2008). One-lung overventilation does not induce inflammation in the normally ventilated contralateral lung Respiratory Physiology & Neurobiology 162, 1, 100-102

Farre, R., Nacher, M., Serrano-Mollar, A., Galdiz, J. B., Alvarez, F. J., Navajas, D., Montserrat, J. M., (2007). Rat model of chronic recurrent airway obstructions to study the sleep apnea syndrome Sleep 30, 7, 930-933

Rico, Félix, Roca-Cusachs, Pere, Sunyer, Raimon, Farré, Ramon, Navajas, Daniel, (2007). Cell dynamic adhesion and elastic properties probed with cylindrical atomic force microscopy cantilever tips Journal of Molecular Recognition John Wiley & Sons, Ltd. 20, 6, 459-466

Equipment

  • Fluorescence resonance energy transfer (FRET) microscopy
  • Confocal Microcopy
  • Traction Microscopy
  • Live cell fluorescence microscopy
  • Cell stretching
  • Cell culture
  • Magnetic Tweezers
  • Atomic Force Microscopy
  • Surface Micro/Nano-patterning

Collaborations

  • Prof. Ramon Farré
    Unit of Biophysics and Bioengineering, Dept. Physiological Sciences, School of Medicine, University of Barcelona/IDIBAPS, Barcelona, Spain
  • Prof. J. M. Montserrat
    Service of Pneumology, Hospital Clinic/IDIBAPS, Barcelona, Spain
  • Prof. Antoni Bayés-Genis
    Institut del Cor dels Germans Trias I Pujol, Badalona
  • Prof. Daniel Weiss
    Department of Medicine, University of Vermont
  • Prof. A. Artigas
    Intensive Care Service, Hospital Parc Taulí, Sabadell
  • Mauricio Rojas
    Scientific Director of the Simmons Center for Interstitial Lung Diseases, University of Pittsburgh
  • David Gozal
    Chair of the Department of Pediatrics, University of Chicago Medical Center

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