by Keyword: il-6

van Aalen, EA, Rosier, BJHM, Jansen, T, Wouters, SFA, Vermathen, RT, van der Veer, HJ, Lozano, JY, Mughal, S, Fernández-Costa, J, Ramón-Azcón, J, den Toonder, JMJ, Merkx, M, (2023). Integrated Bioluminescent Immunoassays for High-Throughput Sampling and Continuous Monitoring of Cytokines Analytical Chemistry 95, 8922-8931

Immunoassays show great potential for the detection of low levels of cytokines, due to their high sensitivity and excellent specificity. There is a particular demand for biosensors that enable both high-throughput screening and continuous monitoring of clinically relevant cytokines such as interleukin-6 (IL-6) and tumor necrosis factor-α (TNFα). To this end, we here introduce a novel bioluminescent immunoassay based on the ratiometric plug-and-play immunodiagnostics (RAPPID) platform, with an improved intrinsic signal-to-background and an >80-fold increase in the luminescent signal. The new dRAPPID assay, comprising a dimeric protein G adapter connected via a semiflexible linker, was applied to detect the secretion of IL-6 by breast carcinoma cells upon TNFα stimulation and the production of low concentrations of IL-6 (∼18 pM) in an endotoxin-stimulated human 3D muscle tissue model. Moreover, we integrated the dRAPPID assay in a newly developed microfluidic device for the simultaneous and continuous monitoring of changes in IL-6 and TNFα in the low-nanomolar range. The luminescence-based read-out and the homogeneous nature of the dRAPPID platform allowed for detection with a simple measurement setup, consisting of a digital camera and a light-sealed box. This permits the usage of the continuous dRAPPID monitoring chip at the point of need, without the requirement for complex or expensive detection techniques.

JTD Keywords: cells, code, elisa, il-6, inflammation, kits, pathogenesis, procalcitonin, release, Interleukin-6

Parra-Monreal, V, Ortega-Machuca, MA, Ramin-Azcin, J, Svendsen, W, Romano-Rodriguez, A, Moreno-Sereno, M, (2021). Detection of cytokines in skeletal muscle tissue using optical SPR sensing platform Proceedings Of The 2021 13th Spanish Conference On Electron Devices, Cde 2021 , 102-105

In this work we have explored the use of a Surface Plasmon resonance (SPR) phenomenon for the detection of interleukin-6 (IL-6), a pro-inflammatory cytokine. It plays an important role in the muscle tissues, having direct relation with muscle contraction and, thus, it is considered a biomarker for some types of muscular dystrophies. Here we show that SPR can be used as a real-time monitoring of the shift of the reflectance dip of a gold diffraction grating in front to the antibody adhesion to gold.

JTD Keywords: antibodies, gratings, interleukin-6 (il-6), proteins, Antibodies, Gratings, Interleukin-6 (il-6), Proteins, Surface plasmon resonance

Manca, M. L., Lattuada, D., Valenti, D., Marelli, O., Corradini, C., Fernàndez-Busquets, X., Zaru, M., Maccioni, A. M., Fadda, A. M., Manconi, M., (2019). Potential therapeutic effect of curcumin loaded hyalurosomes against inflammatory and oxidative processes involved in the pathogenesis of rheumatoid arthritis: The use of fibroblast-like synovial cells cultured in synovial fluid European Journal of Pharmaceutics and Biopharmaceutics 136, 84-92

In the present work curcumin loaded hyalurosomes were proposed as innovative systems for the treatment of rheumatoid arthritis. Vesicles were prepared using a one-step and environmentally friendly method. Aiming at finding the most suitable formulation in terms of size, surface charge and stability on storage, an extensive pre-formulation study was performed using different type and amount of phospholipids. Curcumin loaded vesicles prepared with 180 mg/ml of Phospholipon 90G (P90G) and immobilized with sodium hyaluronate (2 mg/ml) were selected because of their small size (189 nm), homogeneous dispersion (PI 0.24), negative charge (−35 mV), suitable ability to incorporate high amount of curcumin (E% 88%) and great stability on storage. The in vitro study using fibroblast-like synovial cells cultured in synovial fluid, demonstrated the ability of these vesicles to downregulate the production of anti-apoptotic proteins IAP1 and IAP2 and stimulate the production of IL-10, while the production of IL-6 and IL-15 and reactive oxygen species was reduced, confirming their suitability in counteracting pathogenesis of rheumatoid arthritis.

JTD Keywords: Curcumin, IL-6 and IL-15, In vitro inflammation, Oxidative stress, Phospholipid vesicles, Synoviocytes