by Keyword: particle-size

Espanol, Montserrat, Davis, Emilia, Meslet, Eliott, Mestres, Gemma, Montufar, Edgar B., Ginebra, Maria-Pau, (2023). Effect of moisture on the reactivity of alpha-tricalcium phosphate Ceramics International 49, 18228-18237

Lolo FN, Walani N, Seemann E, Zalvidea D, Pavón DM, Cojoc G, Zamai M, Viaris de Lesegno C, Martínez de Benito F, Sánchez-Álvarez M, Uriarte JJ, Echarri A, Jiménez-Carretero D, Escolano JC, Sánchez SA, Caiolfa VR, Navajas D, Trepat X, Guck J, Lamaze C, Roca-Cusachs P, Kessels MM, Qualmann B, Arroyo M, Del Pozo MA, (2023). Caveolin-1 dolines form a distinct and rapid caveolae-independent mechanoadaptation system Nature Cell Biology 25, 120-133

In response to different types and intensities of mechanical force, cells modulate their physical properties and adapt their plasma membrane (PM). Caveolae are PM nano-invaginations that contribute to mechanoadaptation, buffering tension changes. However, whether core caveolar proteins contribute to PM tension accommodation independently from the caveolar assembly is unknown. Here we provide experimental and computational evidence supporting that caveolin-1 confers deformability and mechanoprotection independently from caveolae, through modulation of PM curvature. Freeze-fracture electron microscopy reveals that caveolin-1 stabilizes non-caveolar invaginations-dolines-capable of responding to low-medium mechanical forces, impacting downstream mechanotransduction and conferring mechanoprotection to cells devoid of caveolae. Upon cavin-1/PTRF binding, doline size is restricted and membrane buffering is limited to relatively high forces, capable of flattening caveolae. Thus, caveolae and dolines constitute two distinct albeit complementary components of a buffering system that allows cells to adapt efficiently to a broad range of mechanical stimuli.© 2022. The Author(s).

JTD Keywords: cavin, cell-migration, cholesterol, extracellular-matrix, nanoscale organization, particle-size, polarization, size distribution, tension, Plasma-membrane