During the last decade, intestinal organoids have emerged as a crucial tool to study intestinal biology in vitro. However, their sphere-like geometry limits the access to the organoid’s lumen hampering their use in many functional experiments where independent access to the different sides of the epithelium is required.
Researchers at IBEC led by Dr. Elena Martínez, in collaboration with IRB, described in a paper published in Scientific Reports a simple method to generate intestinal epithelial monolayers on thin Matrigel® films.
These monolayers recapitulate the in vivo-like cell type composition and organization in a tissue culture format that allows independent access to the apical and basolateral compartments. As a unique characteristic, these epithelial monolayers form functional epithelial barriers with an accessible surface and physiologically relevant electrical properties.
This technology offers an up-to-date physiologically relevant intestinal culture method compatible with functional assays such as drug absorption, intracellular trafficking and microbe-epithelium interaction assays.