by Keyword: Cell line, tumor
Fernandes, S, de la Cruz, JO, Morazzo, S, Niro, F, Duríková, H, Cassani, M, Caravella, A, Fiore, P, Azzato, G, De Marco, G, Lauria, A, Izzi, V, Bosáková, V, Fric, J, Filipensky, P, Forte, G, (2024). TGF-β induces matrisome pathological alterations and EMT in patient-derived prostate cancer tumoroids Matrix Biology 125, 12-30
Extracellular matrix (ECM) tumorigenic alterations resulting in high matrix deposition and stiffening are hallmarks of adenocarcinomas and are collectively defined as desmoplasia. Here, we thoroughly analysed primary prostate cancer tissues obtained from numerous patients undergoing radical prostatectomy to highlight reproducible structural changes in the ECM leading to the loss of the glandular architecture. Starting from patient cells, we established prostate cancer tumoroids (PCTs) and demonstrated they require TGF-beta signalling pathway activity to preserve phenotypical and structural similarities with the tissue of origin. By modulating TGF-beta signalling pathway in PCTs, we unveiled its role in ECM accumulation and remodelling in prostate cancer. We also found that TGF-beta-induced ECM remodelling is responsible for the initiation of prostate cell epithelial-tomesenchymal transition (EMT) and the acquisition of a migratory, invasive phenotype. Our findings highlight the cooperative role of TGF-beta signalling and ECM desmoplasia in prompting prostate cell EMT and promoting tumour progression and dissemination.
JTD Keywords: Cancer, Cancer,tumour,extracellular matrix (ecm),tumoroids,prostate,epithelial-to-mesenchymal transition (emt),transforming growth factor (tgf, Cell line, tumor, Epithelial-mesenchymal transition, Epithelial-to-mesenchymal transition (emt), Extracellular matrix, Extracellular matrix (ecm), Humans, Male, Mesenchymal transition emt,tenascin-c,cell-migration,quality-control,expressio, Prostate, Prostatic neoplasms, Transforming growth factor (tgf)-β, Transforming growth factor beta, Tumoroids, Tumour
Garcia, J, Fernández-Pradas, JM, Lladó, A, Serra, P, Zalvidea, D, Kogan, MJ, Giralt, E, Sánchez-Navarro, M, (2021). The Combined Use of Gold Nanoparticles and Infrared Radiation Enables Cytosolic Protein Delivery Chemistry-A European Journal 27, 4670-4675 
© 2020 Wiley-VCH GmbH Cytosolic protein delivery remains elusive. The inability of most proteins to cross the cellular membrane is a huge hurdle. Here we explore the unique photothermal properties of gold nanorods (AuNRs) to trigger cytosolic delivery of proteins. Both partners, protein and AuNRs, are modified with a protease-resistant cell-penetrating peptide with nuclear targeting properties to induce internalization. Once internalized, spatiotemporal control of protein release is achieved by near-infrared laser irradiation in the safe second biological window. Importantly, catalytic amounts of AuNRs are sufficient to trigger cytosolic protein delivery. To the best of our knowledge, this is the first time that AuNRs with their maximum of absorption in the second biological window are used to deliver proteins into the intracellular space. This strategy represents a powerful tool for the cytosolic delivery of virtually any class of protein.
JTD Keywords: cell-penetrating peptide, cytosolic delivery, gold nanorod, near-infrared irradiation, Cell line, tumor, Cell-penetrating peptide, Cytosolic delivery, Gold, Gold nanorod, Metal nanoparticles, Nanotubes, Near-infrared irradiation, Phototherapy
