by Keyword: Antifouling

Riedelová, Zuzana, de los Santos Pereira, Andres, Svoboda, Jan, Pop‐Georgievski, Ognen, Májek, Pavel, Pečánková, Klára, Dyčka, Filip, Rodriguez‐Emmenegger, Cesar, Riedel, Tomáš, (2022). The Relation Between Protein Adsorption and Hemocompatibility of Antifouling Polymer Brushes Macromolecular Bioscience 22, 2200247

Whenever an artificial surface comes into contact with blood, proteins are rapidly adsorbed onto its surface. This phenomenon, termed fouling, is then followed by a series of undesired reactions involving activation of complement or the coagulation cascade and adhesion of leukocytes and platelets leading to thrombus formation. Thus, considerable efforts are directed towards the preparation of fouling-resistant surfaces with the best possible hemocompatibility. Herein, a comprehensive hemocompatibility study after heparinized blood contact with seven polymer brushes prepared by surface-initiated atom transfer radical polymerization is reported. The resistance to fouling is quantified and thrombus formation and deposition of blood cellular components on the coatings are analyzed. Moreover, identification of the remaining adsorbed proteins is performed via mass spectroscopy to elucidate their influence on the surface hemocompatibility. Compared with an unmodified glass surface, the grafting of polymer brushes minimizes the adhesion of platelets and leukocytes and prevents the thrombus formation. The fouling from undiluted blood plasma is reduced by up to 99%. Most of the identified proteins are connected with the initial events of foreign body reaction towards biomaterial (coagulation cascade proteins, complement component, and inflammatory proteins). In addition, several proteins that are not previously linked with blood-biomaterial interaction are presented and discussed.

JTD Keywords: biosensor, blood-plasma, coagulation, coatings, compatibility, glycoprotein, hemocompatibility, identification, methacrylate), ms identification, polymer brushes, protein adsorption, surface-chemistry, Antifouling surfaces, High-density-lipoprotein

Quandt, Jonas, GaraySarmiento, Manuela, Witzdam, Lena, Englert, Jenny, Rutsch, Yannik, Stöcker, Cornelia, Obstals, Fabian, Grottke, Oliver, RodriguezEmmenegger, Cesar, (2022). Interactive Hemocompatible Nanocoating to Prevent SurfaceInduced Coagulation in Medical Devices Advanced Materials Interfaces 9, 2201055

Credi, C., De Marco, C., Molena, E., Pla Roca, M., Samitier, J., Marques, J., Fernàndez-Busquets, X., Levi, M., Turri, S., (2016). Heparin micropatterning onto fouling-release perfluoropolyether-based polymers via photobiotin activation Colloids and Surfaces B: Biointerfaces 146, 250-259

A simple method for constructing versatile ordered biotin/avidin arrays on UV-curable perfluoropolyethers (PFPEs) is presented. The goal is the realization of a versatile platform where any biotinylated biological ligands can be further linked to the underlying biotin/avidin array. To this end, microcontact arrayer and microcontact printing technologies were developed for photobiotin direct printing on PFPEs. As attested by fluorescence images, we demonstrate that this photoactive form of biotin is capable of grafting onto PFPEs surfaces during irradiation. Bioaffinity conjugation of the biotin/avidin system was subsequently exploited for further self-assembly avidin family proteins onto photobiotin arrays. The excellent fouling release PFPEs surface properties enable performing avidin assembly step simply by arrays incubation without PFPEs surface passivation or chemical modification to avoid unspecific biomolecule adsorption. Finally, as a proof of principle biotinylated heparin was successfully grafted onto photobiotin/avidin arrays.

JTD Keywords: Antifouling, Heparin, Malaria, Microcontact arrayer, Microcontact printing, Micropatterning, Perfluoropolyether, Photobiotin, Polymers, Soft lithography