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Publications

by Keyword: Stents

Chausse, V, Iglesias, C, Bou-Petit, E, Ginebra, MP, Pegueroles, M, (2023). Chemical vs thermal accelerated hydrolytic degradation of 3D-printed PLLA/PLCL bioresorbable stents: Characterization and influence of sterilization Polymer Testing 117, 107817

Bioresorbable stents (BRS) are designed to provide initial sufficient mechanical support to prevent vessel recoil while being degraded until their complete resorption. Therefore, degradation rate of BRS plays a crucial role in successful stent performance. This work presents a complete study on the degradation of poly-llactic acid (PLLA) and poly(lactic-co-epsilon-caprolactone) (PLCL) stents fabricated by solvent-cast direct-writing (SC-DW) through two different accelerated assays: alkaline medium at 37 degrees C for 10 days and PBS at 50 degrees C for 4 months. On retrieval, degraded stents were characterized in terms of mass loss, molecular weight (Mw), thermal and mechanical properties. The results showed that under alkaline conditions, stents underwent surface erosion, whereas stents immersed in PBS at 50 degrees C experienced bulk degradation. M-n decrease was accurately described by the autocatalyzed kinetic model, with PLCL showing a degradation rate 1.5 times higher than PLLA. Additionally, stents were subjected to gamma-irradiation and ethylene oxide (EtO) sterilization. Whereas EtOsterilized stents remained structurally unaltered, gamma-irradiated stents presented severe deterioration as a result of extensive chain scission.

JTD Keywords: Acid, Behavior, Bioresorbable stents, Copolymer, Hydrolytic degradation, In-vitro degradation, Mechanical-properties, Molecular-weight, Poly(l-lactide), Poly-l-lactic acid, Poly-l-lactide, Scaffolds, Solvent-cast direct-writing, Sterilization


Schieber, Romain, Mas-Moruno, Carlos, Lasserre, Federico, Roa, Joan Josep, Ginebra, Maria-Pau, Mücklich, Frank, Pegueroles, Marta, (2022). Effectiveness of Direct Laser Interference Patterning and Peptide Immobilization on Endothelial Cell Migration for Cardio-Vascular Applications: An In Vitro Study Nanomaterials 12, 1217

Endothelial coverage of an exposed cardiovascular stent surface leads to the occurrence of restenosis and late-stent thrombosis several months after implantation. To overcome this difficulty, modification of stent surfaces with topographical or biochemical features may be performed to increase endothelial cells’ (ECs) adhesion and/or migration. This work combines both strategies on cobalt-chromium (CoCr) alloy and studies the potential synergistic effect of linear patterned surfaces that are obtained by direct laser interference patterning (DLIP), coupled with the use of Arg-Gly-Asp (RGD) and Tyr-Ile-Gly-Ser-Arg (YIGSR) peptides. An extensive characterization of the modified surfaces was performed by using AFM, XPS, surface charge, electrochemical analysis and fluorescent methods. The biological response was studied in terms of EC adhesion, migration and proliferation assays. CoCr surfaces were successfully patterned with a periodicity of 10 µm and two different depths, D (≈79 and 762 nm). RGD and YIGSR were immobilized on the surfaces by CPTES silanization. Early EC adhesion was increased on the peptide-functionalized surfaces, especially for YIGSR compared to RGD. High-depth patterns generated 80% of ECs’ alignment within the topographical lines and enhanced EC migration. It is noteworthy that the combined use of the two strategies synergistically accelerated the ECs’ migration and proliferation, proving the potential of this strategy to enhance stent endothelialization.

JTD Keywords: adhesion, bare-metal, biofunctionalization, biomaterials, cell adhesive peptides, cobalt-chromium alloy, endothelial cell migration, functionalization, matrix, proliferation, selectivity, shear-stress, surfaces, Direct laser interference patterning (dlip), Drug-eluting stents


Chausse, Victor, Schieber, Romain, Raymond, Yago, Ségry, Brian, Sabaté, Ramon, Kolandaivelu, Kumaran, Ginebra, Maria-Pau, Pegueroles, Marta, (2021). Solvent-cast direct-writing as a fabrication strategy for radiopaque stents Additive Manufacturing 48,