DONATE

Publications

by Keyword: Micromotors

Chen, SQ, Prado-Morales, C, Sánchez-deAlcázar, D, Sánchez, S, (2024). Enzymatic micro/nanomotors in biomedicine: from single motors to swarms Journal Of Materials Chemistry b 12,

Micro/nanomotors (MNMs) have evolved from single self-propelled entities to versatile systems capable of performing one or multiple biomedical tasks. When single MNMs self-assemble into coordinated swarms, either under external control or triggered by chemical reactions, they offer advantages that individual MNMs cannot achieve. These benefits include intelligent multitasking and adaptability to changes in the surrounding environment. Here, we provide our perspective on the evolution of MNMs, beginning with the development of enzymatic MNMs since the first theoretical model was proposed in 2005. These enzymatic MNMs hold immense promise in biomedicine due to their advantages in biocompatibility and fuel availability. Subsequently, we introduce the design and application of single motors in biomedicine, followed by the control of MNM swarms and their biomedical applications. In the end, we propose viable solutions for advancing the development of MNM swarms and anticipate valuable insights into the creation of more intelligent and controllable MNM swarms for biomedical applications.; Micro/nanomotor swarms propelled by diverse mechanisms.

JTD Keywords: Active particles, Actuation, Behaviors, Biocompatibility, Biomedical applications, Coordination reactions, Design and application, Diffusion, External control, Medical applications, Micromotors, Motion, Nanomotors, Powered nanomotors, Propulsion, Self-assemble, Surrounding environment, Theoretical modeling, Versatile system, Viable solutions


Wang, L, Huang, Y, Xu, H, Chen, S, Chen, H, Lin, Y, Wang, X, Liu, X, Sanchez, S, Huang, X, (2022). Contaminants-fueled laccase-powered Fe3O4@SiO2 nanomotors for synergistical degradation of multiple pollutants Materials Today Chemistry 26, 101059

Although an increasing number of micro/nanomotors have been designed for environmental remediation in the past decade, the construction of contaminants-fueled nanomotors for synergistically degrading multiple pollutants simultaneously remains a challenge. Herein, laccase-powered Fe3O4@silica nanomotors are fabricated, assisted with lipase enzyme for the enhanced degradation of multiple contaminants using the contaminants themselves as fuels. Notably, we demonstrate that representative industrial phenols and polycyclic aromatic pollutants possess the ability of triggering the enhanced Brownian motion of laccase nanomotors (De of 1.16 mu m(2)/s in 220 mu M biphenol A (BPA), 1.40 mu m(2)/s in 375 mu M Congo red (CR)). Additionally, the k(cat) value of lipase-assisted laccase-powered nanomotors increased over 1.4 times, enhancing their Brownian motion, while leading to the efficient degradation of multiple contaminants such as BPA, CR, and triacetin droplets within 40 min, simultaneously. Ultimately, the lipase-assisted laccase nanomotors exhibit great advantages over free laccase, free lipase, lipase nanomotors, or laccase nanomotors in K-m, k(cat), catalytic stability, recycling property, and the degradation efficiency of contaminants. Therefore, our work further broadens the library of enzyme-powered nanomotors and provides deep insights in synergistical enzymatic catalysis, thus paving avenues for environmental remediation based on enzyme-powered micro/nanomotors. (C) 2022 Elsevier Ltd. All rights reserved.

JTD Keywords: core, dye, environmental remediation, enzyme catalysis, hybrid, light, microspheres, motors, pollutants removal, propulsion, removal, self-propulsion, shell, Core, Dye, Environmental remediation, Enzyme catalysis, Hybrid, Light, Micro/nanomotors, Micromotors, Microspheres, Motors, Pollutants removal, Propulsion, Removal, Self-propulsion, Shell


Arque, X, Patino, T, Sanchez, S, (2022). Enzyme-powered micro- and nano-motors: key parameters for an application-oriented design Chemical Science 13, 9128-9146

Nature has inspired the creation of artificial micro- and nanomotors that self-propel converting chemical energy into mechanical action. These tiny machines have appeared as promising biomedical tools for treatment and diagnosis and have also been used for environmental, antimicrobial or sensing applications. Among the possible catalytic engines, enzymes have emerged as an alternative to inorganic catalysts due to their biocompatibility and the variety and bioavailability of fuels. Although the field of enzyme-powered micro- and nano-motors has a trajectory of more than a decade, a comprehensive framework on how to rationally design, control and optimize their motion is still missing. With this purpose, herein we performed a thorough bibliographic study on the key parameters governing the propulsion of these enzyme-powered devices, namely the chassis shape, the material composition, the motor size, the enzyme type, the method used to incorporate enzymes, the distribution of the product released, the motion mechanism, the motion media and the technique used for motion detection. In conclusion, from the library of options that each parameter offers there needs to be a rational selection and intelligent design of enzymatic motors based on the specific application envisioned.

JTD Keywords: Catalase, Hydrogen-peroxide, Micro/nanomotors, Micromotors, Movement, Nanomotors, Propulsion, Surfactants, Therapy, Tumor microenvironment


Vilela, D, Guix, M, Parmar, J, Blanco-Blanes, A, Sánchez, S, (2022). Micromotor‐in‐Sponge Platform for Multicycle Large‐Volume Degradation of Organic Pollutants Small 18, 2107619

The presence of organic pollutants in the environment is a global threat to human health and ecosystems due to their bioaccumulation and long-term persistence. Hereby a micromotor-in-sponge concept is presented that aims not only at pollutant removal, but towards an efficient in situ degradation by exploiting the synergy between the sponge hydrophobic nature and the rapid pollutant degradation promoted by the cobalt-ferrite (CFO) micromotors embedded at the sponge's core. Such a platform allows the use of extremely low fuel concentration (0.13% H2 O2 ), as well as its reusability and easy recovery. Moreover, the authors demonstrate an efficient multicycle pollutant degradation and treatment of large volumes (1 L in 15 min) by using multiple sponges. Such a fast degradation process is due to the CFO bubble-propulsion motion mechanism, which induces both an enhanced fluid mixing within the sponge and an outward flow that allows a rapid fluid exchange. Also, the magnetic control of the system is demonstrated, guiding the sponge position during the degradation process. The micromotor-in-sponge configuration can be extrapolated to other catalytic micromotors, establishing an alternative platform for an easier implementation and recovery of micromotors in real environmental applications.© 2022 Wiley-VCH GmbH.

JTD Keywords: effective removal, fabrication, microbots, microjets, organic pollutants, propelled micromotors, self-propelled micromotors, sponges, water treatment, Oil-water separation, Organic pollutants, Water treatment


Valles, M, Pujals, S, Albertazzi, L, Sánchez, S, (2022). Enzyme Purification Improves the Enzyme Loading, Self-Propulsion, and Endurance Performance of Micromotors Acs Nano 16, 5615-5626

Enzyme-powered micro- and nanomotors make use of biocatalysis to self-propel in aqueous media and hold immense promise for active and targeted drug delivery. Most (if not all) of these micro- and nanomotors described to date are fabricated using a commercially available enzyme, despite claims that some commercial preparations may not have a sufficiently high degree of purity for downstream applications. In this study, the purity of a commercial urease, an enzyme frequently used to power the motion of micro- and nanomotors, was evaluated and found to be impure. After separating the hexameric urease from the protein impurities by size-exclusion chromatography, the hexameric urease was subsequently characterized and used to functionalize hollow silica microcapsules. Micromotors loaded with purified urease were found to be 2.5 times more motile than the same micromotors loaded with unpurified urease, reaching average speeds of 5.5 ?m/s. After comparing a number of parameters, such as enzyme distribution, protein loading, and motor reusability, between micromotors functionalized with purified vs unpurified urease, it was concluded that protein purification was essential for optimal performance of the enzyme-powered micromotor.

JTD Keywords: canavalin, catalysis, delivery, dls, enhanced diffusion, enzyme, lipase immobilization, micromotors, self-propulsion, super-resolution microscopy, urease, Mesoporous silica nanoparticles, Micromotors, Super-resolution microscopy


Vilela, D, Blanco-Cabra, N, Eguskiza, A, Hortelao, AC, Torrents, E, Sanchez, S, (2021). Drug-Free Enzyme-Based Bactericidal Nanomotors against Pathogenic Bacteria Acs Applied Materials & Interfaces 13, 14964-14973

The low efficacy of current conventional treatments for bacterial infections increases mortality rates worldwide. To alleviate this global health problem, we propose drug-free enzyme-based nanomotors for the treatment of bacterial urinary-tract infections. We develop nanomotors consisting of mesoporous silica nanoparticles (MSNPs) that were functionalized with either urease (U-MSNPs), lysozyme (L-MSNPs), or urease and lysozyme (M-MSNPs), and use them against nonpathogenic planktonic Escherichia coli. U-MSNPs exhibited the highest bactericidal activity due to biocatalysis of urea into NaHCO3 and NH3, which also propels U-MSNPs. In addition, U-MSNPs in concentrations above 200 μg/mL were capable of successfully reducing 60% of the biofilm biomass of a uropathogenic E. coli strain. This study thus provides a proof-of-concept, demonstrating that enzyme-based nanomotors are capable of fighting infectious diseases. This approach could potentially be extended to other kinds of diseases by selecting appropriate biomolecules.

JTD Keywords: biofilms, carbonate, e. coli, enzymatic nanomotors, infections, lysozyme, micromotors, nanomachines, proteins, self-propulsion, Biofilms, E. coli, Eliminate escherichia-coli, Enzymatic nanomotors, Infections, Nanomachines, Self-propulsion


Hortelao, AC, Simó, C, Guix, M, Guallar-Garrido, S, Julián, E, Vilela, D, Rejc, L, Ramos-Cabrer, P, Cossío, U, Gómez-Vallejo, V, Patiño, T, Llop, J, Sánchez, S, (2021). Swarming behavior and in vivo monitoring of enzymatic nanomotors within the bladder Science Robotics 6, eabd2823

Enzyme-powered nanomotors are an exciting technology for biomedical applications due to their ability to navigate within biological environments using endogenous fuels. However, limited studies into their collective behavior and demonstrations of tracking enzyme nanomotors in vivo have hindered progress toward their clinical translation. Here, we report the swarming behavior of urease-powered nanomotors and its tracking using positron emission tomography (PET), both in vitro and in vivo. For that, mesoporous silica nanoparticles containing urease enzymes and gold nanoparticles were used as nanomotors. To image them, nanomotors were radiolabeled with either I on gold nanoparticles or F-labeled prosthetic group to urease. In vitro experiments showed enhanced fluid mixing and collective migration of nanomotors, demonstrating higher capability to swim across complex paths inside microfabricated phantoms, compared with inactive nanomotors. In vivo intravenous administration in mice confirmed their biocompatibility at the administered dose and the suitability of PET to quantitatively track nanomotors in vivo. Furthermore, nanomotors were administered directly into the bladder of mice by intravesical injection. When injected with the fuel, urea, a homogeneous distribution was observed even after the entrance of fresh urine. By contrast, control experiments using nonmotile nanomotors (i.e., without fuel or without urease) resulted in sustained phase separation, indicating that the nanomotors’ self-propulsion promotes convection and mixing in living reservoirs. Active collective dynamics, together with the medical imaging tracking, constitute a key milestone and a step forward in the field of biomedical nanorobotics, paving the way toward their use in theranostic applications. 124 18

JTD Keywords: cell, reversal, silica nanoparticles, size, step, transport, Propelled micromotors


Wang, Lei, Hortelão, Ana C., Huang, Xin, Sánchez, Samuel, (2019). Lipase-powered mesoporous silica nanomotors for triglyceride degradation Angewandte Chemie International Edition 58, (24), 7992-7996

We report lipase-based nanomotors that are capable of enhanced Brownian motion over long periods of time in triglyceride solution and of degrading triglyceride droplets that mimic “blood lipids”. We achieved about 40 min of enhanced diffusion of lipase-modified mesoporous silica nanoparticles (MSNPs) through a biocatalytic reaction between lipase and its corresponding water-soluble oil substrate (triacetin) as fuel, which resulted in an enhanced diffusion coefficient (ca. 50 % increase) at low triacetin concentration (<10 mm). Lipase not only serves as the power engine but also as a highly efficient cleaner for the triglyceride droplets (e.g., tributyrin) in PBS solution, which could yield potential biomedical applications, for example, for dealing with diseases related to the accumulation of triglycerides, or for environmental remediation, for example, for the degradation of oil spills.

JTD Keywords: Enzyme nanomotors, Lipase, Micromotors, Oil removal, Self-propulsion


Patiño, Tania, Porchetta, Alessandro, Jannasch, Anita, Lladó, Anna, Stumpp, Tom, Schäffer, Erik, Ricci, Francesco, Sánchez, Samuel, (2019). Self-sensing enzyme-powered micromotors equipped with pH-responsive DNA nanoswitches Nano Letters 19, (6), 3440-3447

Biocatalytic micro- and nanomotors have emerged as a new class of active matter self-propelled through enzymatic reactions. The incorporation of functional nanotools could enable the rational design of multifunctional micromotors for simultaneous real-time monitoring of their environment and activity. Herein, we report the combination of DNA nanotechnology and urease-powered micromotors as multifunctional tools able to swim, simultaneously sense the pH of their surrounding environment, and monitor their intrinsic activity. With this purpose, a FRET-labeled triplex DNA nanoswitch for pH sensing was immobilized onto the surface of mesoporous silica-based micromotors. During self-propulsion, urea decomposition and the subsequent release of ammonia led to a fast pH increase, which was detected by real-time monitoring of the FRET efficiency through confocal laser scanning microscopy at different time points (i.e., 30 s, 2 and 10 min). Furthermore, the analysis of speed, enzymatic activity, and propulsive force displayed a similar exponential decay, matching the trend observed for the FRET efficiency. These results illustrate the potential of using specific DNA nanoswitches not only for sensing the micromotors’ surrounding microenvironment but also as an indicator of the micromotor activity status, which may aid to the understanding of their performance in different media and in different applications.

JTD Keywords: Micromotors, DNA-nanoswitch, pH detection, Self-propulsion, Nanosensors, Nanomotors


Katuri, Jaideep, Caballero, David, Voituriez, R., Samitier, Josep, Sanchez, Samuel, (2018). Directed flow of micromotors through alignment interactions with micropatterned ratchets ACS Nano 12, (7), 7282-7291

To achieve control over naturally diffusive, out-of-equilibrium systems composed of self-propelled particles, such as cells or self-phoretic colloids, is a long-standing challenge in active matter physics. The inherently random motion of these active particles can be rectified in the presence of local and periodic asymmetric cues given that a non-trivial interaction exists between the self-propelled particle and the cues. Here, we exploit the phoretic and hydrodynamic interactions of synthetic micromotors with local topographical features to break the time-reversal symmetry of particle trajectories and to direct a macroscopic flow of micromotors. We show that the orientational alignment induced on the micromotors by the topographical features, together with their geometrical asymmetry, are crucial in generating directional particle flow. We also show that our system can be used to concentrate micromotors in confined spaces and identify the interactions responsible for this effect. Finally, we develop a minimal model which identifies the main parameters of the system responsible for the observed rectification. Overall, our system allows for robust control over both temporal and spatial distribution of synthetic micromotors.

JTD Keywords: Active colloids, Directional control, Janus particles, Micromotors, Self-propulsion


Villa, Katherine, Parmar, Jemish, Vilela, Diana, Sánchez, Samuel, (2018). Metal-oxide-based microjets for the simultaneous removal of organic pollutants and heavy metals ACS Applied Materials and Interfaces 10, (24), 20478-20486

Water contamination from industrial and anthropogenic activities is nowadays a major issue in many countries worldwide. To address this problem, efficient water treatment technologies are required. Recent efforts have focused on the development of self-propelled micromotors that provide enhanced micromixing and mass transfer by the transportation of reactive species, resulting in higher decontamination rates. However, a real application of these micromotors is still limited due to the high cost associated to their fabrication process. Here, we present Fe2O3-decorated SiO2/MnO2 microjets for the simultaneous removal of industrial organic pollutants and heavy metals present in wastewater. These microjets were synthesized by low-cost and scalable methods. They exhibit an average speed of 485 ± 32 μm s–1 (∼28 body length per s) at 7% H2O2, which is the highest reported for MnO2-based tubular micromotors. Furthermore, the photocatalytic and adsorbent properties of the microjets enable the efficient degradation of organic pollutants, such as tetracycline and rhodamine B under visible light irradiation, as well as the removal of heavy metal ions, such as Cd2+ and Pb2+.

JTD Keywords: Micromotors, Photocatalytic, Water purification, Fenton, Magnetic control, Iron oxide, Manganese oxide


Parmar, J., Villa, K., Vilela, D., Sánchez, S., (2017). Platinum-free cobalt ferrite based micromotors for antibiotic removal Applied Materials Today 9, 605-611

Self-propelled micromotors have previously shown to enhance pollutant removal compared to non-motile nano-micro particles. However, these systems are expensive, difficult to scale-up and require surfactant for efficient work. Efficient and inexpensive micromotors are desirable for their practical applications in water treatment technologies. We describe cobalt-ferrite based micromotors (CFO micromotors) fabricated by a facile and scalable synthesis, that produce hydroxyl radicals via Fenton-like reaction and take advantage of oxygen gas generated during this reaction for self-propulsion. Once the reaction is complete, the CFO micromotors can be easily separated and collected due to their magnetic nature. The CFO micromotors are demonstrated for highly efficient advanced oxidative removal of tetracycline antibiotic from the water. Furthermore, the effects of different concentrations of micromotors and hydrogen peroxide on the antibiotic degradation were studied, as well as the generation of the highly reactive hydroxyl radicals responsible for the oxidation reaction.

JTD Keywords: Degradation, Fenton reaction, Microbots, Nanomotors, Self-propelled Micromotors, Water treatment


Stanton, Morgan M., Sánchez, Samuel, (2017). Pushing bacterial biohybrids to in vivo applications Trends in Biotechnology , 35, (10), 910-913

Bacterial biohybrids use the energy of bacteria to manipulate synthetic materials with the goal of solving biomedical problems at the micro- and nanoscale. We explore current in vitro studies of bacterial biohybrids, the first attempts at in vivo biohybrid research, and problems to be addressed for the future.

JTD Keywords: Bacteria, Biohybrid, Microswimmers, Micromotors, Drug delivery


Stanton, M. M., Park, B. W., Miguel-López, A., Ma, X., Sitti, M., Sánchez, S., (2017). Biohybrid microtube swimmers driven by single captured bacteria Small 13, (19), 1603679

Bacteria biohybrids employ the motility and power of swimming bacteria to carry and maneuver microscale particles. They have the potential to perform microdrug and cargo delivery in vivo, but have been limited by poor design, reduced swimming capabilities, and impeded functionality. To address these challenge, motile Escherichia coli are captured inside electropolymerized microtubes, exhibiting the first report of a bacteria microswimmer that does not utilize a spherical particle chassis. Single bacterium becomes partially trapped within the tube and becomes a bioengine to push the microtube though biological media. Microtubes are modified with "smart" material properties for motion control, including a bacteria-attractant polydopamine inner layer, addition of magnetic components for external guidance, and a biochemical kill trigger to cease bacterium swimming on demand. Swimming dynamics of the bacteria biohybrid are quantified by comparing "length of protrusion" of bacteria from the microtubes with respect to changes in angular autocorrelation and swimmer mean squared displacement. The multifunctional microtubular swimmers present a new generation of biocompatible micromotors toward future microbiorobots and minimally invasive medical applications.

JTD Keywords: Biohybrids, E. coli, Micromotors, Microswimmers, Polydopamine


Vilela, D., Stanton, M. M., Parmar, J., Sánchez, S., (2017). Microbots decorated with silver nanoparticles kill bacteria in aqueous media ACS Applied Materials & Interfaces 9, (27), 22093-22100

Water contamination is one of the most persistent problems of public health. Resistance of some pathogens to conventional disinfectants can require the combination of multiple disinfectants or increased disinfectant doses, which may produce harmful byproducts. Here, we describe an efficient method for disinfecting Escherichia coli and removing the bacteria from contaminated water using water self-propelled Janus microbots decorated with silver nanoparticles (AgNPs). The structure of a spherical Janus microbot consists of a magnesium (Mg) microparticle as a template that also functions as propulsion source by producing hydrogen bubbles when in contact with water, an inner iron (Fe) magnetic layer for their remote guidance and collection, and an outer AgNP-coated gold (Au) layer for bacterial adhesion and improving bactericidal properties. The active motion of microbots increases the chances of the contact of AgNPs on the microbot surface with bacteria, which provokes the selective Ag+ release in their cytoplasm, and the microbot self-propulsion increases the diffusion of the released Ag+ ions. In addition, the AgNP-coated Au cap of the microbots has a dual capability of capturing bacteria and then killing them. Thus, we have demonstrated that AgNP-coated Janus microbots are capable of efficiently killing more than 80% of E. coli compared with colloidal AgNPs that killed only less than 35% of E. coli in contaminated water solutions in 15 min. After capture and extermination of bacteria, magnetic properties of the cap allow collection of microbots from water along with the captured dead bacteria, leaving water with no biological contaminants. The presented biocompatible Janus microbots offer an encouraging method for rapid disinfection of water.

JTD Keywords: Bactericidal, Magnetic control, Micromotors, Microswimmers, Self-propulsion, Silver nanoparticles


Parmar, J., Vilela, D., Pellicer, E., Esqué-de los Ojos, D., Sort, J., Sánchez, S., (2016). Reusable and long-lasting active microcleaners for heterogeneous water remediation Advanced Functional Materials 26, (23), 4152-4161

Self-powered micromachines are promising tools for future environmental remediation technology. Waste-water treatment and water reuse is an essential part of environmental sustainability. Herein, we present reusable Fe/Pt multi-functional active microcleaners that are capable of degrading organic pollutants (malachite green and 4-nitrophenol) by generated hydroxyl radicals via a Fenton-like reaction. Various different properties of microcleaners, such as the effect of their size, short-term storage, long-term storage, reusability, continuous swimming capability, surface composition, and mechanical properties, are studied. It is found that these microcleaners can continuously swim for more than 24 hours and can be stored more than 5 weeks during multiple cleaning cycles. The produced microcleaners can also be reused, which reduces the cost of the process. During the reuse cycles the outer iron surface of the Fe/Pt microcleaners generates the in-situ, heterogeneous Fenton catalyst and releases a low concentration of iron into the treated water, while the mechanical properties also appear to be improved due to both its surface composition and structural changes. The microcleaners are characterized by scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), nanoindentation, and finite-element modeling (FEM).

JTD Keywords: Catalysts, Heterogeneous catalysis, Microcleaners, Micromotors, Nanorobots, Wastewater treatment


Sánchez, S., Soler, L., Katuri, J., (2015). Chemically powered micro- and nanomotors Angewandte Chemie - International Edition 54, (4), 1414-1444

Chemically powered micro- and nanomotors are small devices that are self-propelled by catalytic reactions in fluids. Taking inspiration from biomotors, scientists are aiming to find the best architecture for self-propulsion, understand the mechanisms of motion, and develop accurate control over the motion. Remotely guided nanomotors can transport cargo to desired targets, drill into biomaterials, sense their environment, mix or pump fluids, and clean polluted water. This Review summarizes the major advances in the growing field of catalytic nanomotors, which started ten years ago.

JTD Keywords: Catalysis, Micromotors, Nanomotors, Robots, Self-propulsion


Ma, X., Katuri, J., Zeng, Y., Zhao, Y., Sánchez, S., (2015). Surface conductive graphene-wrapped micromotors exhibiting enhanced motion Small 11, (38), 5023–5027

Surface-conductive Janus spherical motors are fabricated by wrapping silica particles with reduced graphene oxide capped with a thin Pt layer. These motors exhibit a 100% enhanced velocity as compared to standard SiO2–Pt motors. Furthermore, the versatility of graphene may open up possibilities for a diverse range of applications from active drug delivery systems to water remediation.

JTD Keywords: Enhanced speed, Graphene wrapping, Janus micromotors, Janus particles, Micromotors, Surface conduction


Khalil, I. S. M., Magdanz, V., Sánchez, S., Schmidt, O. G., Misra, S., (2015). Precise localization and control of catalytic janus micromotors using weak magnetic fields International Journal of Advanced Robotic Systems , 12, (2), 1-7

We experimentally demonstrate the precise localization of spherical Pt-Silica Janus micromotors (diameter 5 μm) under the influence of controlled magnetic fields. First, we control the motion of the Janus micromotors in two-dimensional (2D) space. The control system achieves precise localization within an average region-of-convergence of 7 μm. Second, we show that these micromotors provide sufficient propulsion force, allowing them to overcome drag and gravitational forces and move both downwards and upwards. This propulsion is studied by moving the micromotors in three-dimensional (3D) space. The micromotors move downwards and upwards at average speeds of 19.1 μm/s and 9.8 μm/s, respectively. Moreover, our closed-loop control system achieves localization in 3D space within an average region-of-convergence of 6.3 μm in diameter. The precise motion control and localization of the Janus micromotors in 2D and 3D spaces provides broad possibilities for nanotechnology applications.

JTD Keywords: 3D space, Localization, Magnetic control, Micromotors, Self-propulsion